Mutants of Rhodobacter sphaeroides lacking one or more pigment‐protein complexes and complementation with reaction‐centre, LH1, and LH2 genes
Summary The photosynthetic apparatus of Rhodobacter sphaeroides is comprised of three types of pigment‐protein complex: the photochemical reaction centre and its attendant LH1 and LH2 light‐harvesting complexes. To augment existing deletion/insertion mutants in the genes coding for these complexes w...
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| Veröffentlicht in: | Molecular microbiology 1992-05, Vol.6 (9), p.1173-1184 |
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| container_title | Molecular microbiology |
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| creator | Jones, M. R. Fowler, G. J. S. Gibson, L. C. D. Grief, G. G. Olsen, J. D. Crielaard, W. Hunter, C. N. |
| description | Summary
The photosynthetic apparatus of Rhodobacter sphaeroides is comprised of three types of pigment‐protein complex: the photochemical reaction centre and its attendant LH1 and LH2 light‐harvesting complexes. To augment existing deletion/insertion mutants in the genes coding for these complexes we have constructed two further mutants, one of which is a novel double mutant which is devoid of all three types of complex. We have also constructed vectors for the expression of either LH1, LH2 or reaction‐centre genes. The resulting system allows each pigment‐protein complex to be studied either as part of an intact photosystem or as the sole complex in the cell. In this way we have demonstrated that reaction centres can assemble independently of either light‐harvesting complex in R sphaeroides. In addition, the isolation of derivatives of the deletion/insertion mutants exhibiting spontaneous mutations in carotenoid biosynthesis provides an avenue for examining the role of carotenoids in the assembly of the photo‐synthetic apparatus. We show that the LH1 complex is assembled regardless of the carotenoid background, and that the type of carotenoid present modifies the absorbance of the LH1 bacteriochlorophylls. |
| doi_str_mv | 10.1111/j.1365-2958.1992.tb01556.x |
| format | Article |
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The photosynthetic apparatus of Rhodobacter sphaeroides is comprised of three types of pigment‐protein complex: the photochemical reaction centre and its attendant LH1 and LH2 light‐harvesting complexes. To augment existing deletion/insertion mutants in the genes coding for these complexes we have constructed two further mutants, one of which is a novel double mutant which is devoid of all three types of complex. We have also constructed vectors for the expression of either LH1, LH2 or reaction‐centre genes. The resulting system allows each pigment‐protein complex to be studied either as part of an intact photosystem or as the sole complex in the cell. In this way we have demonstrated that reaction centres can assemble independently of either light‐harvesting complex in R sphaeroides. In addition, the isolation of derivatives of the deletion/insertion mutants exhibiting spontaneous mutations in carotenoid biosynthesis provides an avenue for examining the role of carotenoids in the assembly of the photo‐synthetic apparatus. We show that the LH1 complex is assembled regardless of the carotenoid background, and that the type of carotenoid present modifies the absorbance of the LH1 bacteriochlorophylls.</description><identifier>ISSN: 0950-382X</identifier><identifier>EISSN: 1365-2958</identifier><identifier>DOI: 10.1111/j.1365-2958.1992.tb01556.x</identifier><identifier>PMID: 1588816</identifier><language>eng</language><publisher>Oxford, UK: Blackwell Publishing Ltd</publisher><subject>Bacteriology ; Biochemistry & Molecular Biology ; Biological and medical sciences ; Carotenoids - genetics ; Conjugation, Genetic ; Escherichia coli ; Fundamental and applied biological sciences. Psychology ; Genetic Complementation Test ; Genetic Vectors ; Life Sciences & Biomedicine ; Metabolism. Enzymes ; Microbiology ; Mutagenesis ; Photosynthetic Reaction Center Complex Proteins - genetics ; Plasmids ; Restriction Mapping ; Rhodobacter sphaeroides ; Rhodobacter sphaeroides - genetics ; Science & Technology</subject><ispartof>Molecular microbiology, 1992-05, Vol.6 (9), p.1173-1184</ispartof><rights>1992 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>true</woscitedreferencessubscribed><woscitedreferencescount>156</woscitedreferencescount><woscitedreferencesoriginalsourcerecordid>wosA1992HT32400012</woscitedreferencesoriginalsourcerecordid><citedby>FETCH-LOGICAL-c4303-3fb0c58a2497b873b26a3b7aa8feed29cad9b07936fae33a6fc07bc4e1f067d83</citedby><cites>FETCH-LOGICAL-c4303-3fb0c58a2497b873b26a3b7aa8feed29cad9b07936fae33a6fc07bc4e1f067d83</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1111%2Fj.1365-2958.1992.tb01556.x$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1111%2Fj.1365-2958.1992.tb01556.x$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>315,781,785,1418,27197,27929,27930,45579,45580</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=5317530$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/1588816$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Jones, M. R.</creatorcontrib><creatorcontrib>Fowler, G. J. S.</creatorcontrib><creatorcontrib>Gibson, L. C. D.</creatorcontrib><creatorcontrib>Grief, G. G.</creatorcontrib><creatorcontrib>Olsen, J. D.</creatorcontrib><creatorcontrib>Crielaard, W.</creatorcontrib><creatorcontrib>Hunter, C. N.</creatorcontrib><title>Mutants of Rhodobacter sphaeroides lacking one or more pigment‐protein complexes and complementation with reaction‐centre, LH1, and LH2 genes</title><title>Molecular microbiology</title><addtitle>MOL MICROBIOL</addtitle><addtitle>Mol Microbiol</addtitle><description>Summary
The photosynthetic apparatus of Rhodobacter sphaeroides is comprised of three types of pigment‐protein complex: the photochemical reaction centre and its attendant LH1 and LH2 light‐harvesting complexes. To augment existing deletion/insertion mutants in the genes coding for these complexes we have constructed two further mutants, one of which is a novel double mutant which is devoid of all three types of complex. We have also constructed vectors for the expression of either LH1, LH2 or reaction‐centre genes. The resulting system allows each pigment‐protein complex to be studied either as part of an intact photosystem or as the sole complex in the cell. In this way we have demonstrated that reaction centres can assemble independently of either light‐harvesting complex in R sphaeroides. In addition, the isolation of derivatives of the deletion/insertion mutants exhibiting spontaneous mutations in carotenoid biosynthesis provides an avenue for examining the role of carotenoids in the assembly of the photo‐synthetic apparatus. We show that the LH1 complex is assembled regardless of the carotenoid background, and that the type of carotenoid present modifies the absorbance of the LH1 bacteriochlorophylls.</description><subject>Bacteriology</subject><subject>Biochemistry & Molecular Biology</subject><subject>Biological and medical sciences</subject><subject>Carotenoids - genetics</subject><subject>Conjugation, Genetic</subject><subject>Escherichia coli</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Genetic Complementation Test</subject><subject>Genetic Vectors</subject><subject>Life Sciences & Biomedicine</subject><subject>Metabolism. Enzymes</subject><subject>Microbiology</subject><subject>Mutagenesis</subject><subject>Photosynthetic Reaction Center Complex Proteins - genetics</subject><subject>Plasmids</subject><subject>Restriction Mapping</subject><subject>Rhodobacter sphaeroides</subject><subject>Rhodobacter sphaeroides - genetics</subject><subject>Science & Technology</subject><issn>0950-382X</issn><issn>1365-2958</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1992</creationdate><recordtype>article</recordtype><sourceid>EZCTM</sourceid><sourceid>EIF</sourceid><recordid>eNqVkcGO0zAQhi0EWkrhEZAshLiwCXbcOA4XtKqArtQKCS0SN8txJq1LYnftVNu98QjwijwJDonKFXyxRv_3z2jmR-gFJSmN780-pYznSVbmIqVlmaV9RWie8_T0AM3O0kM0I2VOEiayr4_RkxD2hFBGOLtAFzQXQlA-Qz83x17ZPmDX4M87V7tK6R48DoedAu9MDQG3Sn8zdoudBew87pwHfDDbDmz_6_uPg3c9GIu16w4tnCKvbD1VA6J64yy-M_0Oe4jNYxVdOioeLvF6RS__GNarDG_BQniKHjWqDfBs-ufoy4f3N8tVsv708Xp5tU70ghGWsKYiOhcqW5RFJQpWZVyxqlBKNAB1VmpVlxUpSsYbBYwp3mhSVHoBtCG8qAWbo1dj37jA7RFCLzsTNLStsuCOQVKe8ULwAXw7gtq7EDw08uBNp_y9pEQOeci9HI4uh6PLIQ855SFP0fx8mnKsOqj_WscAov5y0lXQqm28stqEM5YzWuRx3Tl6PWJ3ULkmaANWw5m6Goaubli2IDHiLNLi3-mlGSNauqPto_XdZDUt3P_HpnKzuaa0YOw34wHOJA</recordid><startdate>199205</startdate><enddate>199205</enddate><creator>Jones, M. R.</creator><creator>Fowler, G. J. S.</creator><creator>Gibson, L. C. D.</creator><creator>Grief, G. G.</creator><creator>Olsen, J. D.</creator><creator>Crielaard, W.</creator><creator>Hunter, C. N.</creator><general>Blackwell Publishing Ltd</general><general>Wiley</general><general>Blackwell Science</general><scope>BLEPL</scope><scope>DTL</scope><scope>EZCTM</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>P64</scope><scope>RC3</scope></search><sort><creationdate>199205</creationdate><title>Mutants of Rhodobacter sphaeroides lacking one or more pigment‐protein complexes and complementation with reaction‐centre, LH1, and LH2 genes</title><author>Jones, M. R. ; Fowler, G. J. S. ; Gibson, L. C. D. ; Grief, G. G. ; Olsen, J. D. ; Crielaard, W. ; Hunter, C. N.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4303-3fb0c58a2497b873b26a3b7aa8feed29cad9b07936fae33a6fc07bc4e1f067d83</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1992</creationdate><topic>Bacteriology</topic><topic>Biochemistry & Molecular Biology</topic><topic>Biological and medical sciences</topic><topic>Carotenoids - genetics</topic><topic>Conjugation, Genetic</topic><topic>Escherichia coli</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Genetic Complementation Test</topic><topic>Genetic Vectors</topic><topic>Life Sciences & Biomedicine</topic><topic>Metabolism. Enzymes</topic><topic>Microbiology</topic><topic>Mutagenesis</topic><topic>Photosynthetic Reaction Center Complex Proteins - genetics</topic><topic>Plasmids</topic><topic>Restriction Mapping</topic><topic>Rhodobacter sphaeroides</topic><topic>Rhodobacter sphaeroides - genetics</topic><topic>Science & Technology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Jones, M. R.</creatorcontrib><creatorcontrib>Fowler, G. J. S.</creatorcontrib><creatorcontrib>Gibson, L. C. D.</creatorcontrib><creatorcontrib>Grief, G. G.</creatorcontrib><creatorcontrib>Olsen, J. D.</creatorcontrib><creatorcontrib>Crielaard, W.</creatorcontrib><creatorcontrib>Hunter, C. N.</creatorcontrib><collection>Web of Science Core Collection</collection><collection>Science Citation Index Expanded</collection><collection>Web of Science - Science Citation Index Expanded - 1992</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><jtitle>Molecular microbiology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Jones, M. R.</au><au>Fowler, G. J. S.</au><au>Gibson, L. C. D.</au><au>Grief, G. G.</au><au>Olsen, J. D.</au><au>Crielaard, W.</au><au>Hunter, C. N.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Mutants of Rhodobacter sphaeroides lacking one or more pigment‐protein complexes and complementation with reaction‐centre, LH1, and LH2 genes</atitle><jtitle>Molecular microbiology</jtitle><stitle>MOL MICROBIOL</stitle><addtitle>Mol Microbiol</addtitle><date>1992-05</date><risdate>1992</risdate><volume>6</volume><issue>9</issue><spage>1173</spage><epage>1184</epage><pages>1173-1184</pages><issn>0950-382X</issn><eissn>1365-2958</eissn><abstract>Summary
The photosynthetic apparatus of Rhodobacter sphaeroides is comprised of three types of pigment‐protein complex: the photochemical reaction centre and its attendant LH1 and LH2 light‐harvesting complexes. To augment existing deletion/insertion mutants in the genes coding for these complexes we have constructed two further mutants, one of which is a novel double mutant which is devoid of all three types of complex. We have also constructed vectors for the expression of either LH1, LH2 or reaction‐centre genes. The resulting system allows each pigment‐protein complex to be studied either as part of an intact photosystem or as the sole complex in the cell. In this way we have demonstrated that reaction centres can assemble independently of either light‐harvesting complex in R sphaeroides. In addition, the isolation of derivatives of the deletion/insertion mutants exhibiting spontaneous mutations in carotenoid biosynthesis provides an avenue for examining the role of carotenoids in the assembly of the photo‐synthetic apparatus. We show that the LH1 complex is assembled regardless of the carotenoid background, and that the type of carotenoid present modifies the absorbance of the LH1 bacteriochlorophylls.</abstract><cop>Oxford, UK</cop><pub>Blackwell Publishing Ltd</pub><pmid>1588816</pmid><doi>10.1111/j.1365-2958.1992.tb01556.x</doi><tpages>12</tpages></addata></record> |
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| ispartof | Molecular microbiology, 1992-05, Vol.6 (9), p.1173-1184 |
| issn | 0950-382X 1365-2958 |
| language | eng |
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| source | Web of Science - Science Citation Index Expanded - 1992<img src="https://exlibris-pub.s3.amazonaws.com/fromwos-v2.jpg" />; MEDLINE; Access via Wiley Online Library |
| subjects | Bacteriology Biochemistry & Molecular Biology Biological and medical sciences Carotenoids - genetics Conjugation, Genetic Escherichia coli Fundamental and applied biological sciences. Psychology Genetic Complementation Test Genetic Vectors Life Sciences & Biomedicine Metabolism. Enzymes Microbiology Mutagenesis Photosynthetic Reaction Center Complex Proteins - genetics Plasmids Restriction Mapping Rhodobacter sphaeroides Rhodobacter sphaeroides - genetics Science & Technology |
| title | Mutants of Rhodobacter sphaeroides lacking one or more pigment‐protein complexes and complementation with reaction‐centre, LH1, and LH2 genes |
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