Molecular cloning of higher-plant 3-oxoacyl-(acyl carrier protein) reductase : sequence identities with the nodG-gene product of the nitrogen-fixing soil bacterium Rhizobium meliloti
cDNA clones encoding the fatty-acid- biosynthetic enzyme NADPH-linked 3-oxoacyl-(acyl carrier protein) (ACP) reductase were isolated from a Brassica napus (rape) developing seed library and from an Arabidopsis thaliana (thale cress) leaf library. The N-terminal end of the coding region shows feature...
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| Veröffentlicht in: | Biochemical journal 1992-04, Vol.283 (2), p.321-326 |
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| container_title | Biochemical journal |
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| creator | SLABAS, R CHASE, D NISHIDA, I MURATA, N SIDEBOTTOM, C SAFFORD, R SHELDON, P KEKWICK, R. G. O HARDIE, D. G MACKINTOSH, R. W |
| description | cDNA clones encoding the fatty-acid- biosynthetic enzyme NADPH-linked 3-oxoacyl-(acyl carrier protein) (ACP) reductase were isolated from a Brassica napus (rape) developing seed library and from an Arabidopsis thaliana (thale cress) leaf library. The N-terminal end of the coding region shows features typical of a stromal-targeting plastid-transit peptide. The deduced amino acid sequences have 41% and 55% identity respectively with the nodG-gene product of Rhizobium meliloti, one of the host-specific genes that restrict infectivity of this bacterium to a small range of host plants. The probability that the nodG-gene product is a oxoreductase strengthens the hypothesis that some of the host-specific nod-gene products are enzymes which synthesize polyketides that uniquely modify the Rhizobium nodulation signal molecule. |
| doi_str_mv | 10.1042/bj2830321 |
| format | Article |
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G. O ; HARDIE, D. G ; MACKINTOSH, R. W</creator><creatorcontrib>SLABAS, R ; CHASE, D ; NISHIDA, I ; MURATA, N ; SIDEBOTTOM, C ; SAFFORD, R ; SHELDON, P ; KEKWICK, R. G. O ; HARDIE, D. G ; MACKINTOSH, R. W</creatorcontrib><description>cDNA clones encoding the fatty-acid- biosynthetic enzyme NADPH-linked 3-oxoacyl-(acyl carrier protein) (ACP) reductase were isolated from a Brassica napus (rape) developing seed library and from an Arabidopsis thaliana (thale cress) leaf library. The N-terminal end of the coding region shows features typical of a stromal-targeting plastid-transit peptide. The deduced amino acid sequences have 41% and 55% identity respectively with the nodG-gene product of Rhizobium meliloti, one of the host-specific genes that restrict infectivity of this bacterium to a small range of host plants. The probability that the nodG-gene product is a oxoreductase strengthens the hypothesis that some of the host-specific nod-gene products are enzymes which synthesize polyketides that uniquely modify the Rhizobium nodulation signal molecule.</description><identifier>ISSN: 0264-6021</identifier><identifier>EISSN: 1470-8728</identifier><identifier>DOI: 10.1042/bj2830321</identifier><identifier>PMID: 1575676</identifier><language>eng</language><publisher>Colchester: Portland Press</publisher><subject>3-Oxoacyl-(Acyl-Carrier-Protein) Reductase ; Alcohol Oxidoreductases - genetics ; Alcohol Oxidoreductases - isolation & purification ; Amino Acid Sequence ; Analytical, structural and metabolic biochemistry ; Arabidopsis thaliana ; Base Sequence ; Biological and medical sciences ; Brassica - enzymology ; Brassica - genetics ; Brassica napus ; cDNA ; Cloning, Molecular - methods ; Enzymes and enzyme inhibitors ; Fundamental and applied biological sciences. Psychology ; Gene Library ; genes ; Genes, Bacterial ; Molecular Sequence Data ; nucleotide sequence ; Oxidoreductases ; Peptide Mapping ; Plants - enzymology ; Plants - genetics ; predictions ; Sequence Homology, Nucleic Acid ; Sinorhizobium meliloti - genetics</subject><ispartof>Biochemical journal, 1992-04, Vol.283 (2), p.321-326</ispartof><rights>1992 INIST-CNRS</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c430t-ac3433ba22c173c7e5693e80c1576d416049bdc2cec38ccaf126fe0963a3434d3</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC1131036/pdf/$$EPDF$$P50$$Gpubmedcentral$$H</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC1131036/$$EHTML$$P50$$Gpubmedcentral$$H</linktohtml><link.rule.ids>230,314,727,780,784,885,27924,27925,53791,53793</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=5297648$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/1575676$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>SLABAS, R</creatorcontrib><creatorcontrib>CHASE, D</creatorcontrib><creatorcontrib>NISHIDA, I</creatorcontrib><creatorcontrib>MURATA, N</creatorcontrib><creatorcontrib>SIDEBOTTOM, C</creatorcontrib><creatorcontrib>SAFFORD, R</creatorcontrib><creatorcontrib>SHELDON, P</creatorcontrib><creatorcontrib>KEKWICK, R. G. O</creatorcontrib><creatorcontrib>HARDIE, D. G</creatorcontrib><creatorcontrib>MACKINTOSH, R. W</creatorcontrib><title>Molecular cloning of higher-plant 3-oxoacyl-(acyl carrier protein) reductase : sequence identities with the nodG-gene product of the nitrogen-fixing soil bacterium Rhizobium meliloti</title><title>Biochemical journal</title><addtitle>Biochem J</addtitle><description>cDNA clones encoding the fatty-acid- biosynthetic enzyme NADPH-linked 3-oxoacyl-(acyl carrier protein) (ACP) reductase were isolated from a Brassica napus (rape) developing seed library and from an Arabidopsis thaliana (thale cress) leaf library. The N-terminal end of the coding region shows features typical of a stromal-targeting plastid-transit peptide. The deduced amino acid sequences have 41% and 55% identity respectively with the nodG-gene product of Rhizobium meliloti, one of the host-specific genes that restrict infectivity of this bacterium to a small range of host plants. The probability that the nodG-gene product is a oxoreductase strengthens the hypothesis that some of the host-specific nod-gene products are enzymes which synthesize polyketides that uniquely modify the Rhizobium nodulation signal molecule.</description><subject>3-Oxoacyl-(Acyl-Carrier-Protein) Reductase</subject><subject>Alcohol Oxidoreductases - genetics</subject><subject>Alcohol Oxidoreductases - isolation & purification</subject><subject>Amino Acid Sequence</subject><subject>Analytical, structural and metabolic biochemistry</subject><subject>Arabidopsis thaliana</subject><subject>Base Sequence</subject><subject>Biological and medical sciences</subject><subject>Brassica - enzymology</subject><subject>Brassica - genetics</subject><subject>Brassica napus</subject><subject>cDNA</subject><subject>Cloning, Molecular - methods</subject><subject>Enzymes and enzyme inhibitors</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Gene Library</subject><subject>genes</subject><subject>Genes, Bacterial</subject><subject>Molecular Sequence Data</subject><subject>nucleotide sequence</subject><subject>Oxidoreductases</subject><subject>Peptide Mapping</subject><subject>Plants - enzymology</subject><subject>Plants - genetics</subject><subject>predictions</subject><subject>Sequence Homology, Nucleic Acid</subject><subject>Sinorhizobium meliloti - genetics</subject><issn>0264-6021</issn><issn>1470-8728</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1992</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFUs1u1DAQjhCoLIUDD4DkA0L0YPBfnKQHJFRBQSpCQnCOnMlk48qJF9uBlgfj-XDY1QInLral72e-GU9RPObsBWdKvOyuRS2ZFPxOseGqYrSuRH232DChFdVM8PvFgxivGeOKKXZSnPCyKnWlN8XPD94hLM4EAs7Pdt4SP5DRbkcMdOfMnIik_sYbuHX0-XoSMCFYDGQXfEI7n5GA_QLJRCTnJOLXBWdAYnuck00WI_lu00jSiGT2_SXd4oyrdtWstX4DNgWfATrYmzVC9NaRzkDCYJeJfBrtD9-trwmddT7Zh8W9wbiIjw73afHl7ZvPF-_o1cfL9xevrygoyRI1IJWUnRECeCWhwlI3EmsGuX_dK66ZaroeBCDIGsAMXOgBWaOlyULVy9Pi1d53t3QT9pB7Csa1u2AnE25bb2z7LzLbsd36by3nkjOps8Gzg0HweTIxtZONgC5PFv0S20o0ohRK_ZfINW8awWQmnu2JEHyMAYdjGs7adRva4zZk7pO_4_9h7r8_408PuIlg3BDMDDYeaaVoKq1q-QsMyMEe</recordid><startdate>19920415</startdate><enddate>19920415</enddate><creator>SLABAS, R</creator><creator>CHASE, D</creator><creator>NISHIDA, I</creator><creator>MURATA, N</creator><creator>SIDEBOTTOM, C</creator><creator>SAFFORD, R</creator><creator>SHELDON, P</creator><creator>KEKWICK, R. G. O</creator><creator>HARDIE, D. G</creator><creator>MACKINTOSH, R. W</creator><general>Portland Press</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>7TM</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>M81</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>19920415</creationdate><title>Molecular cloning of higher-plant 3-oxoacyl-(acyl carrier protein) reductase : sequence identities with the nodG-gene product of the nitrogen-fixing soil bacterium Rhizobium meliloti</title><author>SLABAS, R ; CHASE, D ; NISHIDA, I ; MURATA, N ; SIDEBOTTOM, C ; SAFFORD, R ; SHELDON, P ; KEKWICK, R. G. O ; HARDIE, D. G ; MACKINTOSH, R. 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Psychology</topic><topic>Gene Library</topic><topic>genes</topic><topic>Genes, Bacterial</topic><topic>Molecular Sequence Data</topic><topic>nucleotide sequence</topic><topic>Oxidoreductases</topic><topic>Peptide Mapping</topic><topic>Plants - enzymology</topic><topic>Plants - genetics</topic><topic>predictions</topic><topic>Sequence Homology, Nucleic Acid</topic><topic>Sinorhizobium meliloti - genetics</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>SLABAS, R</creatorcontrib><creatorcontrib>CHASE, D</creatorcontrib><creatorcontrib>NISHIDA, I</creatorcontrib><creatorcontrib>MURATA, N</creatorcontrib><creatorcontrib>SIDEBOTTOM, C</creatorcontrib><creatorcontrib>SAFFORD, R</creatorcontrib><creatorcontrib>SHELDON, P</creatorcontrib><creatorcontrib>KEKWICK, R. G. O</creatorcontrib><creatorcontrib>HARDIE, D. G</creatorcontrib><creatorcontrib>MACKINTOSH, R. 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G. O</au><au>HARDIE, D. G</au><au>MACKINTOSH, R. W</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Molecular cloning of higher-plant 3-oxoacyl-(acyl carrier protein) reductase : sequence identities with the nodG-gene product of the nitrogen-fixing soil bacterium Rhizobium meliloti</atitle><jtitle>Biochemical journal</jtitle><addtitle>Biochem J</addtitle><date>1992-04-15</date><risdate>1992</risdate><volume>283</volume><issue>2</issue><spage>321</spage><epage>326</epage><pages>321-326</pages><issn>0264-6021</issn><eissn>1470-8728</eissn><abstract>cDNA clones encoding the fatty-acid- biosynthetic enzyme NADPH-linked 3-oxoacyl-(acyl carrier protein) (ACP) reductase were isolated from a Brassica napus (rape) developing seed library and from an Arabidopsis thaliana (thale cress) leaf library. The N-terminal end of the coding region shows features typical of a stromal-targeting plastid-transit peptide. The deduced amino acid sequences have 41% and 55% identity respectively with the nodG-gene product of Rhizobium meliloti, one of the host-specific genes that restrict infectivity of this bacterium to a small range of host plants. The probability that the nodG-gene product is a oxoreductase strengthens the hypothesis that some of the host-specific nod-gene products are enzymes which synthesize polyketides that uniquely modify the Rhizobium nodulation signal molecule.</abstract><cop>Colchester</cop><pub>Portland Press</pub><pmid>1575676</pmid><doi>10.1042/bj2830321</doi><tpages>6</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0264-6021 |
| ispartof | Biochemical journal, 1992-04, Vol.283 (2), p.321-326 |
| issn | 0264-6021 1470-8728 |
| language | eng |
| recordid | cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_1131036 |
| source | MEDLINE; EZB-FREE-00999 freely available EZB journals; PubMed Central; Alma/SFX Local Collection |
| subjects | 3-Oxoacyl-(Acyl-Carrier-Protein) Reductase Alcohol Oxidoreductases - genetics Alcohol Oxidoreductases - isolation & purification Amino Acid Sequence Analytical, structural and metabolic biochemistry Arabidopsis thaliana Base Sequence Biological and medical sciences Brassica - enzymology Brassica - genetics Brassica napus cDNA Cloning, Molecular - methods Enzymes and enzyme inhibitors Fundamental and applied biological sciences. Psychology Gene Library genes Genes, Bacterial Molecular Sequence Data nucleotide sequence Oxidoreductases Peptide Mapping Plants - enzymology Plants - genetics predictions Sequence Homology, Nucleic Acid Sinorhizobium meliloti - genetics |
| title | Molecular cloning of higher-plant 3-oxoacyl-(acyl carrier protein) reductase : sequence identities with the nodG-gene product of the nitrogen-fixing soil bacterium Rhizobium meliloti |
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