IMMUNOCYTOCHEMICAL LOCALIZATION OF PHOTOSYSTEM-I AND THE FUCOXANTHIN-CHLOROPHYLL-A/C LIGHT-HARVESTING COMPLEX IN THE DIATOM PHAEODACTYLUM-TRICORNUTUM
Antiserum against two polypeptides of the major fucoxanthin-chlorophyll a/c light-harvesting complex of the diatom Phaeodactylum tricornutum and heterologous antiserum against purified photosystem I particles of maize were used to localize these two complexes on the thylakoid membranes of P. tricorn...
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Veröffentlicht in: | Protoplasma 1992-01, Vol.166 (3-4), p.208-217 |
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description | Antiserum against two polypeptides of the major fucoxanthin-chlorophyll a/c light-harvesting complex of the diatom Phaeodactylum tricornutum and heterologous antiserum against purified photosystem I particles of maize were used to localize these two complexes on the thylakoid membranes of P. tricornutum. As in many chromophyte algae, the thylakoids are loosely appressed and organized into extended bands of three, giving a ratio of 2:1 for appressed versus non-appressed membranes. Immunoelectron microscopy demonstrated that the fucoxanthin-chlorophyll a/c light-harvesting complex, which is believed to be associated with photosystem II, was equally distributed on the appressed and non-appressed thylakoid membranes. Photosystem I was also found on both types of membranes, but was slightly more concentrated on the two outer non-appressed membranes of each band. Similarly, photosystem I activity, as measured by the photooxidation of 3,3'-diaminobenzidine, was higher in the outer thylakoids than in the central thylakoid of each band. We conclude that the thylakoids of diatoms differ from those of green algae and higher plants in their macromolecular organization as well as in their morphological arrangement. |
doi_str_mv | 10.1007/BF01322783 |
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As in many chromophyte algae, the thylakoids are loosely appressed and organized into extended bands of three, giving a ratio of 2:1 for appressed versus non-appressed membranes. Immunoelectron microscopy demonstrated that the fucoxanthin-chlorophyll a/c light-harvesting complex, which is believed to be associated with photosystem II, was equally distributed on the appressed and non-appressed thylakoid membranes. Photosystem I was also found on both types of membranes, but was slightly more concentrated on the two outer non-appressed membranes of each band. Similarly, photosystem I activity, as measured by the photooxidation of 3,3'-diaminobenzidine, was higher in the outer thylakoids than in the central thylakoid of each band. 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As in many chromophyte algae, the thylakoids are loosely appressed and organized into extended bands of three, giving a ratio of 2:1 for appressed versus non-appressed membranes. Immunoelectron microscopy demonstrated that the fucoxanthin-chlorophyll a/c light-harvesting complex, which is believed to be associated with photosystem II, was equally distributed on the appressed and non-appressed thylakoid membranes. Photosystem I was also found on both types of membranes, but was slightly more concentrated on the two outer non-appressed membranes of each band. Similarly, photosystem I activity, as measured by the photooxidation of 3,3'-diaminobenzidine, was higher in the outer thylakoids than in the central thylakoid of each band. We conclude that the thylakoids of diatoms differ from those of green algae and higher plants in their macromolecular organization as well as in their morphological arrangement.</description><subject>Biological and medical sciences</subject><subject>Cell Biology</subject><subject>Cell structures and functions</subject><subject>Chloroplast, photosynthetic membrane and photosynthesis</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Life Sciences & Biomedicine</subject><subject>Metabolism</subject><subject>Molecular and cellular biology</subject><subject>Phaeodactylum tricornutum</subject><subject>Photosynthesis, respiration. 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Psychology</topic><topic>Life Sciences & Biomedicine</topic><topic>Metabolism</topic><topic>Molecular and cellular biology</topic><topic>Phaeodactylum tricornutum</topic><topic>Photosynthesis, respiration. Anabolism, catabolism</topic><topic>Plant physiology and development</topic><topic>Plant Sciences</topic><topic>Science & Technology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>PYSZNIAK, AM</creatorcontrib><creatorcontrib>GIBBS, SP</creatorcontrib><collection>Web of Science Core Collection</collection><collection>Science Citation Index Expanded</collection><collection>Web of Science - Science Citation Index Expanded - 1992</collection><collection>Pascal-Francis</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><jtitle>Protoplasma</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>PYSZNIAK, AM</au><au>GIBBS, SP</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>IMMUNOCYTOCHEMICAL LOCALIZATION OF PHOTOSYSTEM-I AND THE FUCOXANTHIN-CHLOROPHYLL-A/C LIGHT-HARVESTING COMPLEX IN THE DIATOM PHAEODACTYLUM-TRICORNUTUM</atitle><jtitle>Protoplasma</jtitle><stitle>PROTOPLASMA</stitle><date>1992-01-01</date><risdate>1992</risdate><volume>166</volume><issue>3-4</issue><spage>208</spage><epage>217</epage><pages>208-217</pages><issn>0033-183X</issn><eissn>1615-6102</eissn><coden>PROTA5</coden><abstract>Antiserum against two polypeptides of the major fucoxanthin-chlorophyll a/c light-harvesting complex of the diatom Phaeodactylum tricornutum and heterologous antiserum against purified photosystem I particles of maize were used to localize these two complexes on the thylakoid membranes of P. tricornutum. As in many chromophyte algae, the thylakoids are loosely appressed and organized into extended bands of three, giving a ratio of 2:1 for appressed versus non-appressed membranes. Immunoelectron microscopy demonstrated that the fucoxanthin-chlorophyll a/c light-harvesting complex, which is believed to be associated with photosystem II, was equally distributed on the appressed and non-appressed thylakoid membranes. Photosystem I was also found on both types of membranes, but was slightly more concentrated on the two outer non-appressed membranes of each band. Similarly, photosystem I activity, as measured by the photooxidation of 3,3'-diaminobenzidine, was higher in the outer thylakoids than in the central thylakoid of each band. We conclude that the thylakoids of diatoms differ from those of green algae and higher plants in their macromolecular organization as well as in their morphological arrangement.</abstract><cop>VIENNA</cop><pub>Springer Nature</pub><doi>10.1007/BF01322783</doi><tpages>10</tpages></addata></record> |
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subjects | Biological and medical sciences Cell Biology Cell structures and functions Chloroplast, photosynthetic membrane and photosynthesis Fundamental and applied biological sciences. Psychology Life Sciences & Biomedicine Metabolism Molecular and cellular biology Phaeodactylum tricornutum Photosynthesis, respiration. Anabolism, catabolism Plant physiology and development Plant Sciences Science & Technology |
title | IMMUNOCYTOCHEMICAL LOCALIZATION OF PHOTOSYSTEM-I AND THE FUCOXANTHIN-CHLOROPHYLL-A/C LIGHT-HARVESTING COMPLEX IN THE DIATOM PHAEODACTYLUM-TRICORNUTUM |
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