Stimulation of Na Absorption by the Antiasthmatic Kampo Drug Saiboku-To in Cultured Airway Epithelium

Stimulation of Na Absorption by the Antiasthmatic Kampo Drug Saiboku-To in Cultured Airway Epithelium

To study the effect of the Kampo drug Saiboku-to (TJ-96) on ion transport function of airway epithelial cells, we studied bioelectric properties of cultured tracheal epithelium from dogs under short-circuit conditions in vitro. Addition of TJ-96 (1 mg/ml) to the mucosal solution of the Ussing chambe...

Ausführliche Beschreibung

Gespeichert in:
Bibliographische Detailangaben
Veröffentlicht in:Japanese Journal of Pharmacology 1992, Vol.58(1), pp.47-53
Hauptverfasser: Tamaoki, Jun, Takeyama, Kiyoshi, Chiyotani, Atsushi, Sakai, Noritaka, Takizawa, Takao, Konno, Kimio
Format: Artikel
Sprache:eng
Schlagworte:
Animals
Biological and medical sciences
Cells, Cultured
Cyclic AMP - analysis
Dogs
Drugs, Chinese Herbal - pharmacology
Epithelium - metabolism
Female
Histamine and antagonists. Allergy
Histamine H1 Antagonists - pharmacology
Life Sciences & Biomedicine
Male
Medical sciences
Medicine, Kampo
Pharmacology & Pharmacy
Pharmacology. Drug treatments
Potentiometry
Science & Technology
Sodium-Potassium-Exchanging ATPase - drug effects
Trachea - metabolism
Online-Zugang:Volltext
Tags: Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
Beschreibung
Zusammenfassung:To study the effect of the Kampo drug Saiboku-to (TJ-96) on ion transport function of airway epithelial cells, we studied bioelectric properties of cultured tracheal epithelium from dogs under short-circuit conditions in vitro. Addition of TJ-96 (1 mg/ml) to the mucosal solution of the Ussing chamber increased the epithelial short-circuit current (SCC) from 6.5 ± 0.7 to 11.4 ± 1.6μA/cm2 (P < 0.001). This effect was dose-dependent, with the maximal increase from the baseline value and the concentration required to produce a half-maximal effect (EC50) being 70.5 ± 12.6% (P
ISSN:0021-5198
1347-3506
DOI:10.1254/jjp.58.47