Development and Evaluation of Stable Sugarcane Mosaic Virus Mild Mutants for Cross-Protection Against Infection by Severe Strain
Sugarcane mosaic virus (SCMV; genus Potyvirus) induces maize dwarf mosaic disease that has caused serious yield losses of maize in China. Cross-protection is one of the efficient strategies to fight against severe virus strains. Although many mild strains have been identified, the spontaneous mutati...
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| Veröffentlicht in: | Frontiers in plant science 2021-12, Vol.12, p.788963-788963, Article 788963 |
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| container_title | Frontiers in plant science |
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| creator | Xu, Xiao-Jie Zhu, Qing Jiang, Shao-Yan Yan, Zhi-Yong Geng, Chao Tian, Yan-Ping Li, Xiang-Dong |
| description | Sugarcane mosaic virus (SCMV; genus Potyvirus) induces maize dwarf mosaic disease that has caused serious yield losses of maize in China. Cross-protection is one of the efficient strategies to fight against severe virus strains. Although many mild strains have been identified, the spontaneous mutation is one of the challenging problems affecting their application in cross-protection. In this study, we found that the substitution of cysteine (C) at positions 57 or 60 in the zinc finger-like motif of HC-Pro with alanine (A; C57A or C60A) significantly reduced its RNA silencing suppression activity and SCMV virulence. To reduce the risk of mild strains mutating to virulent ones by reverse or complementary mutations, we obtained attenuated SCMV mutants with double-mutations in the zinc finger-like and FRNK motifs of HC-Pro and evaluated their potential application in cross-protection. The results showed that the maize plants infected with FKNK/C60A double-mutant showed symptomless until 95 days post-inoculation and FKNK/C60A cross-protected plants displayed high resistance to severe SCMV strain. This study provides theoretical and material bases for the control of SCMV through cross-protection. |
| doi_str_mv | 10.3389/fpls.2021.788963 |
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Cross-protection is one of the efficient strategies to fight against severe virus strains. Although many mild strains have been identified, the spontaneous mutation is one of the challenging problems affecting their application in cross-protection. In this study, we found that the substitution of cysteine (C) at positions 57 or 60 in the zinc finger-like motif of HC-Pro with alanine (A; C57A or C60A) significantly reduced its RNA silencing suppression activity and SCMV virulence. To reduce the risk of mild strains mutating to virulent ones by reverse or complementary mutations, we obtained attenuated SCMV mutants with double-mutations in the zinc finger-like and FRNK motifs of HC-Pro and evaluated their potential application in cross-protection. The results showed that the maize plants infected with FKNK/C60A double-mutant showed symptomless until 95 days post-inoculation and FKNK/C60A cross-protected plants displayed high resistance to severe SCMV strain. This study provides theoretical and material bases for the control of SCMV through cross-protection.</description><identifier>ISSN: 1664-462X</identifier><identifier>EISSN: 1664-462X</identifier><identifier>DOI: 10.3389/fpls.2021.788963</identifier><identifier>PMID: 34975975</identifier><language>eng</language><publisher>LAUSANNE: Frontiers Media Sa</publisher><subject>cross-protection ; helper component-proteinase ; Life Sciences & Biomedicine ; Plant Science ; Plant Sciences ; RNA silencing suppression ; Science & Technology ; spontaneous mutation ; sugarcane mosaic virus ; virulence</subject><ispartof>Frontiers in plant science, 2021-12, Vol.12, p.788963-788963, Article 788963</ispartof><rights>Copyright © 2021 Xu, Zhu, Jiang, Yan, Geng, Tian and Li.</rights><rights>Copyright © 2021 Xu, Zhu, Jiang, Yan, Geng, Tian and Li. 2021 Xu, Zhu, Jiang, Yan, Geng, Tian and Li</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>true</woscitedreferencessubscribed><woscitedreferencescount>13</woscitedreferencescount><woscitedreferencesoriginalsourcerecordid>wos000738844300001</woscitedreferencesoriginalsourcerecordid><citedby>FETCH-LOGICAL-c462t-35261631d70e3994a3ccdf5670cadda65b5dcefe9375edb46e26b6c373d5c6b23</citedby><cites>FETCH-LOGICAL-c462t-35261631d70e3994a3ccdf5670cadda65b5dcefe9375edb46e26b6c373d5c6b23</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC8718998/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC8718998/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,315,728,781,785,865,886,2103,2115,27928,27929,53795,53797</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/34975975$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Xu, Xiao-Jie</creatorcontrib><creatorcontrib>Zhu, Qing</creatorcontrib><creatorcontrib>Jiang, Shao-Yan</creatorcontrib><creatorcontrib>Yan, Zhi-Yong</creatorcontrib><creatorcontrib>Geng, Chao</creatorcontrib><creatorcontrib>Tian, Yan-Ping</creatorcontrib><creatorcontrib>Li, Xiang-Dong</creatorcontrib><title>Development and Evaluation of Stable Sugarcane Mosaic Virus Mild Mutants for Cross-Protection Against Infection by Severe Strain</title><title>Frontiers in plant science</title><addtitle>FRONT PLANT SCI</addtitle><addtitle>Front Plant Sci</addtitle><description>Sugarcane mosaic virus (SCMV; genus Potyvirus) induces maize dwarf mosaic disease that has caused serious yield losses of maize in China. Cross-protection is one of the efficient strategies to fight against severe virus strains. Although many mild strains have been identified, the spontaneous mutation is one of the challenging problems affecting their application in cross-protection. In this study, we found that the substitution of cysteine (C) at positions 57 or 60 in the zinc finger-like motif of HC-Pro with alanine (A; C57A or C60A) significantly reduced its RNA silencing suppression activity and SCMV virulence. To reduce the risk of mild strains mutating to virulent ones by reverse or complementary mutations, we obtained attenuated SCMV mutants with double-mutations in the zinc finger-like and FRNK motifs of HC-Pro and evaluated their potential application in cross-protection. The results showed that the maize plants infected with FKNK/C60A double-mutant showed symptomless until 95 days post-inoculation and FKNK/C60A cross-protected plants displayed high resistance to severe SCMV strain. This study provides theoretical and material bases for the control of SCMV through cross-protection.</description><subject>cross-protection</subject><subject>helper component-proteinase</subject><subject>Life Sciences & Biomedicine</subject><subject>Plant Science</subject><subject>Plant Sciences</subject><subject>RNA silencing suppression</subject><subject>Science & Technology</subject><subject>spontaneous mutation</subject><subject>sugarcane mosaic virus</subject><subject>virulence</subject><issn>1664-462X</issn><issn>1664-462X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2021</creationdate><recordtype>article</recordtype><sourceid>HGBXW</sourceid><sourceid>DOA</sourceid><recordid>eNqNksGPEyEUxidG427WvXsyHE1MKwwMw1xMNnXVJttoUjXeCPOAymYKFZg1e_NPl2lrs3uTkEB4H7_He3xV9ZLgOaWie2t3Q5rXuCbzVoiO0yfVOeGczRivfzx9sD-rLlO6xWU0GHdd-7w6o6xrmzLPqz_vzZ0Zwm5rfEbKa3R9p4ZRZRc8Chats-oHg9bjRkVQ3qBVSMoB-u7imNDKDRqtxqx8TsiGiBYxpDT7EkM2sEdcbZTzKaOlt8eT_h6tS8pYoDmW4IvqmVVDMpfH9aL69uH66-LT7Obzx-Xi6mYGpYY8o03NCadEt9jQrmOKAmjb8BaD0lrxpm80GGs62jZG94ybmvccaEt1A7yv6UW1PHB1ULdyF91WxXsZlJP7gxA3UsXsYDASWrAY2o4IUMwyo8BQ4LQmTGDQCgrr3YG1G_utKXl9KWV4BH0c8e6n3IQ7KVoiuk4UwOsjIIZfo0lZbl0CMwylxWFMcqq1FoRQVqT4IIWpt9HYUxqC5eQDOflATj6QBx-UK68ePu904d-vF8Gbg-C36YNN4IwHc5IVo7RUCMbo5BlS1OL_1QuX9-ZZhNFn-hcgrNOm</recordid><startdate>20211217</startdate><enddate>20211217</enddate><creator>Xu, Xiao-Jie</creator><creator>Zhu, Qing</creator><creator>Jiang, Shao-Yan</creator><creator>Yan, Zhi-Yong</creator><creator>Geng, Chao</creator><creator>Tian, Yan-Ping</creator><creator>Li, Xiang-Dong</creator><general>Frontiers Media Sa</general><general>Frontiers Media S.A</general><scope>BLEPL</scope><scope>DTL</scope><scope>HGBXW</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>5PM</scope><scope>DOA</scope></search><sort><creationdate>20211217</creationdate><title>Development and Evaluation of Stable Sugarcane Mosaic Virus Mild Mutants for Cross-Protection Against Infection by Severe Strain</title><author>Xu, Xiao-Jie ; Zhu, Qing ; Jiang, Shao-Yan ; Yan, Zhi-Yong ; Geng, Chao ; Tian, Yan-Ping ; Li, Xiang-Dong</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c462t-35261631d70e3994a3ccdf5670cadda65b5dcefe9375edb46e26b6c373d5c6b23</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2021</creationdate><topic>cross-protection</topic><topic>helper component-proteinase</topic><topic>Life Sciences & Biomedicine</topic><topic>Plant Science</topic><topic>Plant Sciences</topic><topic>RNA silencing suppression</topic><topic>Science & Technology</topic><topic>spontaneous mutation</topic><topic>sugarcane mosaic virus</topic><topic>virulence</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Xu, Xiao-Jie</creatorcontrib><creatorcontrib>Zhu, Qing</creatorcontrib><creatorcontrib>Jiang, Shao-Yan</creatorcontrib><creatorcontrib>Yan, Zhi-Yong</creatorcontrib><creatorcontrib>Geng, Chao</creatorcontrib><creatorcontrib>Tian, Yan-Ping</creatorcontrib><creatorcontrib>Li, Xiang-Dong</creatorcontrib><collection>Web of Science Core Collection</collection><collection>Science Citation Index Expanded</collection><collection>Web of Science - Science Citation Index Expanded - 2021</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><collection>DOAJ Directory of Open Access Journals</collection><jtitle>Frontiers in plant science</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Xu, Xiao-Jie</au><au>Zhu, Qing</au><au>Jiang, Shao-Yan</au><au>Yan, Zhi-Yong</au><au>Geng, Chao</au><au>Tian, Yan-Ping</au><au>Li, Xiang-Dong</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Development and Evaluation of Stable Sugarcane Mosaic Virus Mild Mutants for Cross-Protection Against Infection by Severe Strain</atitle><jtitle>Frontiers in plant science</jtitle><stitle>FRONT PLANT SCI</stitle><addtitle>Front Plant Sci</addtitle><date>2021-12-17</date><risdate>2021</risdate><volume>12</volume><spage>788963</spage><epage>788963</epage><pages>788963-788963</pages><artnum>788963</artnum><issn>1664-462X</issn><eissn>1664-462X</eissn><abstract>Sugarcane mosaic virus (SCMV; genus Potyvirus) induces maize dwarf mosaic disease that has caused serious yield losses of maize in China. Cross-protection is one of the efficient strategies to fight against severe virus strains. Although many mild strains have been identified, the spontaneous mutation is one of the challenging problems affecting their application in cross-protection. In this study, we found that the substitution of cysteine (C) at positions 57 or 60 in the zinc finger-like motif of HC-Pro with alanine (A; C57A or C60A) significantly reduced its RNA silencing suppression activity and SCMV virulence. To reduce the risk of mild strains mutating to virulent ones by reverse or complementary mutations, we obtained attenuated SCMV mutants with double-mutations in the zinc finger-like and FRNK motifs of HC-Pro and evaluated their potential application in cross-protection. The results showed that the maize plants infected with FKNK/C60A double-mutant showed symptomless until 95 days post-inoculation and FKNK/C60A cross-protected plants displayed high resistance to severe SCMV strain. 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| subjects | cross-protection helper component-proteinase Life Sciences & Biomedicine Plant Science Plant Sciences RNA silencing suppression Science & Technology spontaneous mutation sugarcane mosaic virus virulence |
| title | Development and Evaluation of Stable Sugarcane Mosaic Virus Mild Mutants for Cross-Protection Against Infection by Severe Strain |
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