Single‐cell RNA sequencing reveals the landscapes of human cord blood hematopoietic stem cell differentiation during ex vivo culture
Here, we established an expansion strategy using a combination of cytokines (SCF, TPO, FLT3-L and IL-6) and USK (UM171, SR1 and K1) that contributed to a 543-fold increase relative to the initial cell population in CD34+CD38–CD45RA–CD90+ cells (Figure 1A–C, Tables S1 and S2). [...]colony-forming uni...
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| creator | Wen, Ruiting Xu, Chen Dong, Chen Sheng, Hongxia Zhao, Long Yang, Yang Zhang, Ang Wang, Shenyu Wang, Li Ju, Yan Liu, Yang Duan, Lian Hu, Liangding Chen, Hu Yang, Zhigang Zhang, Bin |
| description | Here, we established an expansion strategy using a combination of cytokines (SCF, TPO, FLT3-L and IL-6) and USK (UM171, SR1 and K1) that contributed to a 543-fold increase relative to the initial cell population in CD34+CD38–CD45RA–CD90+ cells (Figure 1A–C, Tables S1 and S2). [...]colony-forming units assay confirmed that the cells cultured with USK, UM171 or K1 retain the ability to differentiate into multilineage progenitors (Figure 1D, Table S3). SEE PDF] Recent studies hold that the cell-surface markers used for isolation of HSCs are inaccurate for ex vivo culture of HSCs.8 Similar to our study, Chen et al.9 found that ex vivo expanded cells with a phenotype of CD34+CD38–CD45RA–CD90+CD49f+ were not functional HSCs. [...]it may be inappropriate to previously define CD34+CD38–CD45RA–CD90+ as a marker for long-term hematopoietic stem cells (LT-HSCs) during ex vivo culture. [...]we analyzed HSC-related transcription factors and their target genes. |
| doi_str_mv | 10.1002/ctm2.616 |
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[...]colony-forming units assay confirmed that the cells cultured with USK, UM171 or K1 retain the ability to differentiate into multilineage progenitors (Figure 1D, Table S3). SEE PDF] Recent studies hold that the cell-surface markers used for isolation of HSCs are inaccurate for ex vivo culture of HSCs.8 Similar to our study, Chen et al.9 found that ex vivo expanded cells with a phenotype of CD34+CD38–CD45RA–CD90+CD49f+ were not functional HSCs. [...]it may be inappropriate to previously define CD34+CD38–CD45RA–CD90+ as a marker for long-term hematopoietic stem cells (LT-HSCs) during ex vivo culture. [...]we analyzed HSC-related transcription factors and their target genes.</description><identifier>ISSN: 2001-1326</identifier><identifier>EISSN: 2001-1326</identifier><identifier>DOI: 10.1002/ctm2.616</identifier><identifier>PMID: 34841734</identifier><language>eng</language><publisher>United States: John Wiley & Sons, Inc</publisher><subject>Cell cycle ; Cell differentiation ; Cytokines ; Expansion ; Fetal Blood - cytology ; Fetal Blood - metabolism ; Gene expression ; Genotype & phenotype ; Hematopoietic Stem Cells - cytology ; Hematopoietic Stem Cells - physiology ; Humans ; Letter to Editor ; RNA ; RNA - analysis ; RNA - blood ; RNA sequencing ; Science ; Single-Cell Analysis - methods ; Single-Cell Analysis - statistics & numerical data ; Stem cell research ; Stem cells ; Transcription factors ; Whole Genome Sequencing - methods ; Whole Genome Sequencing - statistics & numerical data</subject><ispartof>Clinical and Translational Medicine, 2021-11, Vol.11 (11), p.e616-n/a</ispartof><rights>2021 The Authors. published by John Wiley & Sons Australia, Ltd on behalf of Shanghai Institute of Clinical Bioinformatics</rights><rights>COPYRIGHT 2021 John Wiley & Sons, Inc.</rights><rights>2021. This work is published under http://creativecommons.org/licenses/by/4.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4736-781a6049d2f01d5529139a27dd67863b1dfc3ef23ad83f88ad30a8fb7b3c9d063</citedby><cites>FETCH-LOGICAL-c4736-781a6049d2f01d5529139a27dd67863b1dfc3ef23ad83f88ad30a8fb7b3c9d063</cites><orcidid>0000-0001-6210-580X</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC8574970/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC8574970/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,724,777,781,861,882,1412,2096,11543,27905,27906,45555,45556,46033,46457,53772,53774</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/34841734$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Wen, Ruiting</creatorcontrib><creatorcontrib>Xu, Chen</creatorcontrib><creatorcontrib>Dong, Chen</creatorcontrib><creatorcontrib>Sheng, Hongxia</creatorcontrib><creatorcontrib>Zhao, Long</creatorcontrib><creatorcontrib>Yang, Yang</creatorcontrib><creatorcontrib>Zhang, Ang</creatorcontrib><creatorcontrib>Wang, Shenyu</creatorcontrib><creatorcontrib>Wang, Li</creatorcontrib><creatorcontrib>Ju, Yan</creatorcontrib><creatorcontrib>Liu, Yang</creatorcontrib><creatorcontrib>Duan, Lian</creatorcontrib><creatorcontrib>Hu, Liangding</creatorcontrib><creatorcontrib>Chen, Hu</creatorcontrib><creatorcontrib>Yang, Zhigang</creatorcontrib><creatorcontrib>Zhang, Bin</creatorcontrib><title>Single‐cell RNA sequencing reveals the landscapes of human cord blood hematopoietic stem cell differentiation during ex vivo culture</title><title>Clinical and Translational Medicine</title><addtitle>Clin Transl Med</addtitle><description>Here, we established an expansion strategy using a combination of cytokines (SCF, TPO, FLT3-L and IL-6) and USK (UM171, SR1 and K1) that contributed to a 543-fold increase relative to the initial cell population in CD34+CD38–CD45RA–CD90+ cells (Figure 1A–C, Tables S1 and S2). [...]colony-forming units assay confirmed that the cells cultured with USK, UM171 or K1 retain the ability to differentiate into multilineage progenitors (Figure 1D, Table S3). SEE PDF] Recent studies hold that the cell-surface markers used for isolation of HSCs are inaccurate for ex vivo culture of HSCs.8 Similar to our study, Chen et al.9 found that ex vivo expanded cells with a phenotype of CD34+CD38–CD45RA–CD90+CD49f+ were not functional HSCs. [...]it may be inappropriate to previously define CD34+CD38–CD45RA–CD90+ as a marker for long-term hematopoietic stem cells (LT-HSCs) during ex vivo culture. [...]we analyzed HSC-related transcription factors and their target genes.</description><subject>Cell cycle</subject><subject>Cell differentiation</subject><subject>Cytokines</subject><subject>Expansion</subject><subject>Fetal Blood - cytology</subject><subject>Fetal Blood - metabolism</subject><subject>Gene expression</subject><subject>Genotype & phenotype</subject><subject>Hematopoietic Stem Cells - cytology</subject><subject>Hematopoietic Stem Cells - physiology</subject><subject>Humans</subject><subject>Letter to Editor</subject><subject>RNA</subject><subject>RNA - analysis</subject><subject>RNA - blood</subject><subject>RNA sequencing</subject><subject>Science</subject><subject>Single-Cell Analysis - methods</subject><subject>Single-Cell Analysis - statistics & numerical data</subject><subject>Stem cell research</subject><subject>Stem cells</subject><subject>Transcription factors</subject><subject>Whole Genome Sequencing - methods</subject><subject>Whole Genome Sequencing - statistics & numerical data</subject><issn>2001-1326</issn><issn>2001-1326</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2021</creationdate><recordtype>article</recordtype><sourceid>24P</sourceid><sourceid>WIN</sourceid><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>DOA</sourceid><recordid>eNp1ks9uEzEQxlcIRKtSiSdAlrhwSfC_eO0LUlQVqFRAgnK2vPY4cbS7DvZuoDdOnHlGngRvUkqLhH2wNf78m_lGU1VPCZ4TjOlLO3R0Loh4UB1TjMmMMCoe3rkfVac5b3BZkitV08fVEeOSk5rx4-rHp9CvWvj1_aeFtkUf3y9Rhi8j9LbEUYIdmDajYQ2oNb3L1mwho-jReuxMj2xMDjVtjA6toTND3MYAQ7AoD9ChPdEF7yFBPwQzhNgjN6aJDN_QLuwismM7jAmeVI98SQSnN-dJ9fn1-dXZ29nlhzcXZ8vLmeU1E7NaEiMwV456TNxiQRVhytDaOVFLwRrivGXgKTNOMi-lcQwb6Zu6YVY5LNhJdXHgumg2eptCZ9K1jibofSCmlTapGGhBN40ovPKHNIqXfLIQmVCOMdFQz2lhvTqwtmPTgbPFYzLtPej9lz6s9SrutFzUXNW4AF7cAFIsLc-D7kKemmZ6iGPWtFjlguO99Pk_0k0cU19apWktsCRcqkk1P6hWphgIvY8lry3bQRds7MGHEl8KxRZE1Vz-rcCmmHMCf1s9wXoaLj0Nly7DVaTP7rq9Ff4ZpSKYHQRfS5Lr_4L02dU7OgF_A8rp2m4</recordid><startdate>202111</startdate><enddate>202111</enddate><creator>Wen, Ruiting</creator><creator>Xu, Chen</creator><creator>Dong, Chen</creator><creator>Sheng, Hongxia</creator><creator>Zhao, Long</creator><creator>Yang, Yang</creator><creator>Zhang, Ang</creator><creator>Wang, Shenyu</creator><creator>Wang, Li</creator><creator>Ju, Yan</creator><creator>Liu, Yang</creator><creator>Duan, Lian</creator><creator>Hu, Liangding</creator><creator>Chen, Hu</creator><creator>Yang, Zhigang</creator><creator>Zhang, Bin</creator><general>John Wiley & Sons, Inc</general><general>John Wiley and Sons Inc</general><general>Wiley</general><scope>24P</scope><scope>WIN</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>IAO</scope><scope>3V.</scope><scope>7X7</scope><scope>7XB</scope><scope>8AO</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AN0</scope><scope>AZQEC</scope><scope>BENPR</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>K9.</scope><scope>M0S</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>7X8</scope><scope>5PM</scope><scope>DOA</scope><orcidid>https://orcid.org/0000-0001-6210-580X</orcidid></search><sort><creationdate>202111</creationdate><title>Single‐cell RNA sequencing reveals the landscapes of human cord blood hematopoietic stem cell differentiation during ex vivo culture</title><author>Wen, Ruiting ; Xu, Chen ; Dong, Chen ; Sheng, Hongxia ; Zhao, Long ; Yang, Yang ; Zhang, Ang ; Wang, Shenyu ; Wang, Li ; Ju, Yan ; Liu, Yang ; Duan, Lian ; Hu, Liangding ; Chen, Hu ; Yang, Zhigang ; Zhang, Bin</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4736-781a6049d2f01d5529139a27dd67863b1dfc3ef23ad83f88ad30a8fb7b3c9d063</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2021</creationdate><topic>Cell cycle</topic><topic>Cell differentiation</topic><topic>Cytokines</topic><topic>Expansion</topic><topic>Fetal Blood - 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[...]colony-forming units assay confirmed that the cells cultured with USK, UM171 or K1 retain the ability to differentiate into multilineage progenitors (Figure 1D, Table S3). SEE PDF] Recent studies hold that the cell-surface markers used for isolation of HSCs are inaccurate for ex vivo culture of HSCs.8 Similar to our study, Chen et al.9 found that ex vivo expanded cells with a phenotype of CD34+CD38–CD45RA–CD90+CD49f+ were not functional HSCs. [...]it may be inappropriate to previously define CD34+CD38–CD45RA–CD90+ as a marker for long-term hematopoietic stem cells (LT-HSCs) during ex vivo culture. [...]we analyzed HSC-related transcription factors and their target genes.</abstract><cop>United States</cop><pub>John Wiley & Sons, Inc</pub><pmid>34841734</pmid><doi>10.1002/ctm2.616</doi><tpages>6</tpages><orcidid>https://orcid.org/0000-0001-6210-580X</orcidid><oa>free_for_read</oa></addata></record> |
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| subjects | Cell cycle Cell differentiation Cytokines Expansion Fetal Blood - cytology Fetal Blood - metabolism Gene expression Genotype & phenotype Hematopoietic Stem Cells - cytology Hematopoietic Stem Cells - physiology Humans Letter to Editor RNA RNA - analysis RNA - blood RNA sequencing Science Single-Cell Analysis - methods Single-Cell Analysis - statistics & numerical data Stem cell research Stem cells Transcription factors Whole Genome Sequencing - methods Whole Genome Sequencing - statistics & numerical data |
| title | Single‐cell RNA sequencing reveals the landscapes of human cord blood hematopoietic stem cell differentiation during ex vivo culture |
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