Advancement in leishmaniasis diagnosis and therapeutics: An update

Leishmaniasis is regarded as a neglected tropical disease by World Health Organization (WHO) and is ranked next to malaria as the deadliest protozoan disease. The primary causative agents of the disease comprise of diverse leishmanial species sharing clinical features ranging from skin abrasions to lethal infection in the visceral organs. As several Leishmania species are involved in infection, the role of accurate diagnosis becomes pivotal in adding new dimensions to anti-leishmanial therapy. Diagnostic methods must be fast, reliable, easy to perform, highly sensitive, and specific to differentiate among similar parasitic diseases. Herein, we present the conventional and recent approaches impended for the disease diagnosis and their sensitivity, specificity, and clinical application in parasite detection. Furthermore, we have also elaborated various new methods to cure leishmaniasis, which include host-directed therapies, drug repurposing, nanotechnology, and combinational therapy. This review addresses novel techniques and innovations in leishmaniasis, which can aid in unraveling new strategies to fight against the deadly infection. •Leishmaniasis is a neglected tropical disease and is regarded as a global health issue by WHO•Fast and accurate diagnosis of this deadly disease is essential to reduce morbidity and mortality•Recent diagnostic approaches such as proteomics, flow cytometry, nano-diagnosis, and advanced molecular techniques have proved to be revolutionary•Novel treatment strategies such as host-directed therapy, drug repurposing, and nanotechnology are the future of leishmaniasis treatment