Small proline-rich protein 2A is a gut bactericidal protein deployed during helminth infection

A diverse group of antimicrobial proteins (AMPs) helps protect the mammalian intestine from varied microbial challenges. We show that small proline-rich protein 2A (SPRR2A) is an intestinal antibacterial protein that is phylogenetically unrelated to previously discovered mammalian AMPs. In this stud...

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Veröffentlicht in:Science (American Association for the Advancement of Science) 2021-11, Vol.374 (6568), p.eabe6723-eabe6723, Article 6723
Hauptverfasser: Hu, Zehan, Zhang, Chenlu, Sifuentes-Dominguez, Luis, Zarek, Christina M., Propheter, Daniel C., Kuang, Zheng, Wang, Yuhao, Pendse, Mihir, Ruhn, Kelly A., Hassell, Brian, Behrendt, Cassie L., Zhang, Bo, Raj, Prithvi, Harris-Tryon, Tamia A., Reese, Tiffany A., Hooper, Lora
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container_issue 6568
container_start_page eabe6723
container_title Science (American Association for the Advancement of Science)
container_volume 374
creator Hu, Zehan
Zhang, Chenlu
Sifuentes-Dominguez, Luis
Zarek, Christina M.
Propheter, Daniel C.
Kuang, Zheng
Wang, Yuhao
Pendse, Mihir
Ruhn, Kelly A.
Hassell, Brian
Behrendt, Cassie L.
Zhang, Bo
Raj, Prithvi
Harris-Tryon, Tamia A.
Reese, Tiffany A.
Hooper, Lora
description A diverse group of antimicrobial proteins (AMPs) helps protect the mammalian intestine from varied microbial challenges. We show that small proline-rich protein 2A (SPRR2A) is an intestinal antibacterial protein that is phylogenetically unrelated to previously discovered mammalian AMPs. In this study, SPRR2A was expressed in Paneth cells and goblet cells and selectively killed Gram-positive bacteria by disrupting their membranes. SPRR2A shaped intestinal microbiota composition, restricted bacterial association with the intestinal surface, and protected against Listeria monocytogenes infection. SPRR2A differed from other intestinal AMPs in that it was induced by type 2 cytokines produced during helminth infection. Moreover, SPRR2A protected against helminth-induced bacterial invasion of intestinal tissue. Thus, SPRR2A is a distinctive AMP triggered by type 2 immunity that protects the intestinal barrier during helminth infection.
doi_str_mv 10.1126/science.abe6723
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We show that small proline-rich protein 2A (SPRR2A) is an intestinal antibacterial protein that is phylogenetically unrelated to previously discovered mammalian AMPs. In this study, SPRR2A was expressed in Paneth cells and goblet cells and selectively killed Gram-positive bacteria by disrupting their membranes. SPRR2A shaped intestinal microbiota composition, restricted bacterial association with the intestinal surface, and protected against Listeria monocytogenes infection. SPRR2A differed from other intestinal AMPs in that it was induced by type 2 cytokines produced during helminth infection. Moreover, SPRR2A protected against helminth-induced bacterial invasion of intestinal tissue. Thus, SPRR2A is a distinctive AMP triggered by type 2 immunity that protects the intestinal barrier during helminth infection.</description><identifier>ISSN: 0036-8075</identifier><identifier>EISSN: 1095-9203</identifier><identifier>DOI: 10.1126/science.abe6723</identifier><identifier>PMID: 34735226</identifier><language>eng</language><publisher>WASHINGTON: Amer Assoc Advancement Science</publisher><subject>Animals ; Antiinfectives and antibacterials ; Bacteria ; Bacterial diseases ; Bacterial infections ; Bacterial Load ; Bactericidal activity ; Biological activity ; Biopsy ; Cell Membrane - metabolism ; Cell Membrane Permeability ; Cell membranes ; Cell walls ; Colonization ; Composition ; Control Groups ; Cornified Envelope Proline-Rich Proteins - genetics ; Cornified Envelope Proline-Rich Proteins - metabolism ; Critical components ; Cytokines ; Cytokines - metabolism ; Damage ; Deactivation ; Digestive system ; Disease Susceptibility ; Disruption ; Epithelium ; Gastrointestinal Microbiome ; Gastrointestinal tract ; Goblet cells ; Goblet Cells - metabolism ; Gram-negative bacteria ; Gram-positive bacteria ; Gram-Positive Bacteria - physiology ; Humans ; Immune system ; Immunity ; Immunity, Innate ; Immunology ; Infections ; Inflammatory bowel diseases ; Interleukin 13 ; Interleukin 4 ; Intestinal microflora ; Intestinal Mucosa - metabolism ; Intestinal Mucosa - microbiology ; Intestine ; Intestines - microbiology ; Land Settlement ; Lipids ; Listeria ; Listeria monocytogenes ; Listeria monocytogenes - physiology ; Listeriosis - microbiology ; Lysozyme ; Mammals ; Membrane structures ; Membranes ; Mice ; Microbial activity ; Microbial Viability ; Microbiota ; Microorganisms ; Mucosa ; Multidisciplinary Sciences ; Nematospiroides dubius ; Paneth cells ; Paneth Cells - metabolism ; Parasites ; Phylogeny ; Pore Forming Cytotoxic Proteins - genetics ; Pore Forming Cytotoxic Proteins - metabolism ; Proline ; Proteins ; Science &amp; Technology ; Science &amp; Technology - Other Topics ; Small intestine ; Strongylida Infections - immunology ; Strongylida Infections - metabolism ; Strongylida Infections - microbiology ; Worms</subject><ispartof>Science (American Association for the Advancement of Science), 2021-11, Vol.374 (6568), p.eabe6723-eabe6723, Article 6723</ispartof><rights>Copyright © 2021 The Authors, some rights reserved; exclusive licensee American Association for the Advancement of Science. 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We show that small proline-rich protein 2A (SPRR2A) is an intestinal antibacterial protein that is phylogenetically unrelated to previously discovered mammalian AMPs. In this study, SPRR2A was expressed in Paneth cells and goblet cells and selectively killed Gram-positive bacteria by disrupting their membranes. SPRR2A shaped intestinal microbiota composition, restricted bacterial association with the intestinal surface, and protected against Listeria monocytogenes infection. SPRR2A differed from other intestinal AMPs in that it was induced by type 2 cytokines produced during helminth infection. Moreover, SPRR2A protected against helminth-induced bacterial invasion of intestinal tissue. Thus, SPRR2A is a distinctive AMP triggered by type 2 immunity that protects the intestinal barrier during helminth infection.</description><subject>Animals</subject><subject>Antiinfectives and antibacterials</subject><subject>Bacteria</subject><subject>Bacterial diseases</subject><subject>Bacterial infections</subject><subject>Bacterial Load</subject><subject>Bactericidal activity</subject><subject>Biological activity</subject><subject>Biopsy</subject><subject>Cell Membrane - metabolism</subject><subject>Cell Membrane Permeability</subject><subject>Cell membranes</subject><subject>Cell walls</subject><subject>Colonization</subject><subject>Composition</subject><subject>Control Groups</subject><subject>Cornified Envelope Proline-Rich Proteins - genetics</subject><subject>Cornified Envelope Proline-Rich Proteins - metabolism</subject><subject>Critical components</subject><subject>Cytokines</subject><subject>Cytokines - metabolism</subject><subject>Damage</subject><subject>Deactivation</subject><subject>Digestive system</subject><subject>Disease Susceptibility</subject><subject>Disruption</subject><subject>Epithelium</subject><subject>Gastrointestinal Microbiome</subject><subject>Gastrointestinal tract</subject><subject>Goblet cells</subject><subject>Goblet Cells - metabolism</subject><subject>Gram-negative bacteria</subject><subject>Gram-positive bacteria</subject><subject>Gram-Positive Bacteria - physiology</subject><subject>Humans</subject><subject>Immune system</subject><subject>Immunity</subject><subject>Immunity, Innate</subject><subject>Immunology</subject><subject>Infections</subject><subject>Inflammatory bowel diseases</subject><subject>Interleukin 13</subject><subject>Interleukin 4</subject><subject>Intestinal microflora</subject><subject>Intestinal Mucosa - metabolism</subject><subject>Intestinal Mucosa - microbiology</subject><subject>Intestine</subject><subject>Intestines - microbiology</subject><subject>Land Settlement</subject><subject>Lipids</subject><subject>Listeria</subject><subject>Listeria monocytogenes</subject><subject>Listeria monocytogenes - physiology</subject><subject>Listeriosis - microbiology</subject><subject>Lysozyme</subject><subject>Mammals</subject><subject>Membrane structures</subject><subject>Membranes</subject><subject>Mice</subject><subject>Microbial activity</subject><subject>Microbial Viability</subject><subject>Microbiota</subject><subject>Microorganisms</subject><subject>Mucosa</subject><subject>Multidisciplinary Sciences</subject><subject>Nematospiroides dubius</subject><subject>Paneth cells</subject><subject>Paneth Cells - metabolism</subject><subject>Parasites</subject><subject>Phylogeny</subject><subject>Pore Forming Cytotoxic Proteins - genetics</subject><subject>Pore Forming Cytotoxic Proteins - metabolism</subject><subject>Proline</subject><subject>Proteins</subject><subject>Science &amp; 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Zhang, Chenlu ; Sifuentes-Dominguez, Luis ; Zarek, Christina M. ; Propheter, Daniel C. ; Kuang, Zheng ; Wang, Yuhao ; Pendse, Mihir ; Ruhn, Kelly A. ; Hassell, Brian ; Behrendt, Cassie L. ; Zhang, Bo ; Raj, Prithvi ; Harris-Tryon, Tamia A. ; Reese, Tiffany A. ; Hooper, Lora</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c421t-e6ce7d12066954f6e661687cc1eecaf1e878468411c576c308fc7eba6960debe3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2021</creationdate><topic>Animals</topic><topic>Antiinfectives and antibacterials</topic><topic>Bacteria</topic><topic>Bacterial diseases</topic><topic>Bacterial infections</topic><topic>Bacterial Load</topic><topic>Bactericidal activity</topic><topic>Biological activity</topic><topic>Biopsy</topic><topic>Cell Membrane - metabolism</topic><topic>Cell Membrane Permeability</topic><topic>Cell membranes</topic><topic>Cell walls</topic><topic>Colonization</topic><topic>Composition</topic><topic>Control Groups</topic><topic>Cornified Envelope Proline-Rich Proteins - genetics</topic><topic>Cornified Envelope Proline-Rich Proteins - metabolism</topic><topic>Critical components</topic><topic>Cytokines</topic><topic>Cytokines - metabolism</topic><topic>Damage</topic><topic>Deactivation</topic><topic>Digestive system</topic><topic>Disease Susceptibility</topic><topic>Disruption</topic><topic>Epithelium</topic><topic>Gastrointestinal Microbiome</topic><topic>Gastrointestinal tract</topic><topic>Goblet cells</topic><topic>Goblet Cells - metabolism</topic><topic>Gram-negative bacteria</topic><topic>Gram-positive bacteria</topic><topic>Gram-Positive Bacteria - physiology</topic><topic>Humans</topic><topic>Immune system</topic><topic>Immunity</topic><topic>Immunity, Innate</topic><topic>Immunology</topic><topic>Infections</topic><topic>Inflammatory bowel diseases</topic><topic>Interleukin 13</topic><topic>Interleukin 4</topic><topic>Intestinal microflora</topic><topic>Intestinal Mucosa - metabolism</topic><topic>Intestinal Mucosa - microbiology</topic><topic>Intestine</topic><topic>Intestines - microbiology</topic><topic>Land Settlement</topic><topic>Lipids</topic><topic>Listeria</topic><topic>Listeria monocytogenes</topic><topic>Listeria monocytogenes - physiology</topic><topic>Listeriosis - microbiology</topic><topic>Lysozyme</topic><topic>Mammals</topic><topic>Membrane structures</topic><topic>Membranes</topic><topic>Mice</topic><topic>Microbial activity</topic><topic>Microbial Viability</topic><topic>Microbiota</topic><topic>Microorganisms</topic><topic>Mucosa</topic><topic>Multidisciplinary Sciences</topic><topic>Nematospiroides dubius</topic><topic>Paneth cells</topic><topic>Paneth Cells - metabolism</topic><topic>Parasites</topic><topic>Phylogeny</topic><topic>Pore Forming Cytotoxic Proteins - genetics</topic><topic>Pore Forming Cytotoxic Proteins - metabolism</topic><topic>Proline</topic><topic>Proteins</topic><topic>Science &amp; 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We show that small proline-rich protein 2A (SPRR2A) is an intestinal antibacterial protein that is phylogenetically unrelated to previously discovered mammalian AMPs. In this study, SPRR2A was expressed in Paneth cells and goblet cells and selectively killed Gram-positive bacteria by disrupting their membranes. SPRR2A shaped intestinal microbiota composition, restricted bacterial association with the intestinal surface, and protected against Listeria monocytogenes infection. SPRR2A differed from other intestinal AMPs in that it was induced by type 2 cytokines produced during helminth infection. Moreover, SPRR2A protected against helminth-induced bacterial invasion of intestinal tissue. Thus, SPRR2A is a distinctive AMP triggered by type 2 immunity that protects the intestinal barrier during helminth infection.</abstract><cop>WASHINGTON</cop><pub>Amer Assoc Advancement Science</pub><pmid>34735226</pmid><doi>10.1126/science.abe6723</doi><tpages>46</tpages><orcidid>https://orcid.org/0000-0002-4539-3720</orcidid><orcidid>https://orcid.org/0000-0003-2325-6546</orcidid><orcidid>https://orcid.org/0000-0002-4170-7083</orcidid><orcidid>https://orcid.org/0000-0002-6211-5132</orcidid><orcidid>https://orcid.org/0000-0002-9525-071X</orcidid><orcidid>https://orcid.org/0000-0002-8665-0885</orcidid><orcidid>https://orcid.org/0000-0002-4648-5301</orcidid><orcidid>https://orcid.org/0000-0002-7810-6791</orcidid><orcidid>https://orcid.org/0000-0003-0800-2353</orcidid><orcidid>https://orcid.org/0000-0001-9462-9237</orcidid><orcidid>https://orcid.org/0000-0001-5855-8371</orcidid><orcidid>https://orcid.org/0000-0002-9440-8546</orcidid><orcidid>https://orcid.org/0000-0002-8231-0875</orcidid><orcidid>https://orcid.org/0000-0002-2759-4641</orcidid><orcidid>https://orcid.org/0000-0003-4068-4358</orcidid><orcidid>https://orcid.org/0000-0002-0031-2173</orcidid><oa>free_for_read</oa></addata></record>
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identifier ISSN: 0036-8075
ispartof Science (American Association for the Advancement of Science), 2021-11, Vol.374 (6568), p.eabe6723-eabe6723, Article 6723
issn 0036-8075
1095-9203
language eng
recordid cdi_webofscience_primary_000714952000028
source MEDLINE; Science Magazine; Web of Science - Science Citation Index Expanded - 2021<img src="https://exlibris-pub.s3.amazonaws.com/fromwos-v2.jpg" />
subjects Animals
Antiinfectives and antibacterials
Bacteria
Bacterial diseases
Bacterial infections
Bacterial Load
Bactericidal activity
Biological activity
Biopsy
Cell Membrane - metabolism
Cell Membrane Permeability
Cell membranes
Cell walls
Colonization
Composition
Control Groups
Cornified Envelope Proline-Rich Proteins - genetics
Cornified Envelope Proline-Rich Proteins - metabolism
Critical components
Cytokines
Cytokines - metabolism
Damage
Deactivation
Digestive system
Disease Susceptibility
Disruption
Epithelium
Gastrointestinal Microbiome
Gastrointestinal tract
Goblet cells
Goblet Cells - metabolism
Gram-negative bacteria
Gram-positive bacteria
Gram-Positive Bacteria - physiology
Humans
Immune system
Immunity
Immunity, Innate
Immunology
Infections
Inflammatory bowel diseases
Interleukin 13
Interleukin 4
Intestinal microflora
Intestinal Mucosa - metabolism
Intestinal Mucosa - microbiology
Intestine
Intestines - microbiology
Land Settlement
Lipids
Listeria
Listeria monocytogenes
Listeria monocytogenes - physiology
Listeriosis - microbiology
Lysozyme
Mammals
Membrane structures
Membranes
Mice
Microbial activity
Microbial Viability
Microbiota
Microorganisms
Mucosa
Multidisciplinary Sciences
Nematospiroides dubius
Paneth cells
Paneth Cells - metabolism
Parasites
Phylogeny
Pore Forming Cytotoxic Proteins - genetics
Pore Forming Cytotoxic Proteins - metabolism
Proline
Proteins
Science & Technology
Science & Technology - Other Topics
Small intestine
Strongylida Infections - immunology
Strongylida Infections - metabolism
Strongylida Infections - microbiology
Worms
title Small proline-rich protein 2A is a gut bactericidal protein deployed during helminth infection
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