The feature of cervical microbiota associated with the progression of cervical cancer among reproductive females
The aim of this study was to characterize cervical microbiome feature of reproductive-age women in the progression of squamous intraepithelial lesions (SIL) to cervical cancer. We characterized the 16S rDNA cervical mucus microbiome in 94 participants (age from 18 to 52), including 13 cervical cance...
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| Veröffentlicht in: | Gynecologic oncology 2021-11, Vol.163 (2), p.348-357 |
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| creator | Wu, Sikao Ding, Xuewen Kong, Ying Acharya, Sanam Wu, Huaqian Huang, Chunning Liang, Yuanyuan Nong, Xianxian Chen, Hong |
| description | The aim of this study was to characterize cervical microbiome feature of reproductive-age women in the progression of squamous intraepithelial lesions (SIL) to cervical cancer.
We characterized the 16S rDNA cervical mucus microbiome in 94 participants (age from 18 to 52), including 13 cervical cancer (CA), 31 high-grade SIL (HSIL), 10 low-grade SIL (LSIL), 12 HPV-infected (NH) patients and 28 healthy controls (NN). Alpha (within sample) diversity was examined by Shannon and Simpson index, while Beta (between sample) diversity by principle coordinate analysis (PCoA) of weighted Unifrac distances. Relative abundance of microbial taxa was compared using Linear Discriminant Analysis Effect Size (LEfSe). Co-occurrence analysis was performed to identify correlation among marker genera, and Phylogenetic investigation of communities by reconstruction of unobserved states (PICRUSt) to explore functional features and pathways of cervical microbiota.
Alpha diversity(p |
| doi_str_mv | 10.1016/j.ygyno.2021.08.016 |
| format | Article |
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We characterized the 16S rDNA cervical mucus microbiome in 94 participants (age from 18 to 52), including 13 cervical cancer (CA), 31 high-grade SIL (HSIL), 10 low-grade SIL (LSIL), 12 HPV-infected (NH) patients and 28 healthy controls (NN). Alpha (within sample) diversity was examined by Shannon and Simpson index, while Beta (between sample) diversity by principle coordinate analysis (PCoA) of weighted Unifrac distances. Relative abundance of microbial taxa was compared using Linear Discriminant Analysis Effect Size (LEfSe). Co-occurrence analysis was performed to identify correlation among marker genera, and Phylogenetic investigation of communities by reconstruction of unobserved states (PICRUSt) to explore functional features and pathways of cervical microbiota.
Alpha diversity(p < 0.05) was higher in severer cervical pathology with lower relative abundance of Lactobacillus as well as higher of anaerobes. Beta diversity (p < 0.01) was significantly different. Marker genera were identified including Porphyromonas, Prevotella and Campylobacter of CA and Sneathia of HSIL. The correlation of differential functional pathways with Prevotella was opposite to that with Lactobacillus.
Our study suggests differences in cervical microbiota diversity and relative abundance of reproductive-age females in different stages of cervical carcinogenesis. Marker genera might participate in the lesion progression and will be helpful for diagnosis, prevention and treatment. These findings may lead the way to further study of the cervical microbiome in development of cervical cancer.
•Cervical species diversity was higher in severer cervical intraepithelial neoplasia of reproductive-age women.•women with severer cervical pathology had more complex composition of cervical microbiota and more anaerobic bacteria.•Porphyromonas and Campylobacter were newfound marker genera of cervical cancer.•The correlation of differential functional pathways with Prevotella was opposite to that with Lactobacillus.•Marker genera in the lesion progression will be helpful for diagnosis, prevention and treatment.</description><identifier>ISSN: 0090-8258</identifier><identifier>EISSN: 1095-6859</identifier><identifier>DOI: 10.1016/j.ygyno.2021.08.016</identifier><identifier>PMID: 34503848</identifier><language>eng</language><publisher>SAN DIEGO: Elsevier Inc</publisher><subject><![CDATA[16S rRNA ; Adult ; Campylobacter - genetics ; Campylobacter - isolation & purification ; Case-Control Studies ; Cervical cancer ; Cervical microbiota ; Cervix Uteri - microbiology ; Cervix Uteri - pathology ; Disease Progression ; DNA, Bacterial - isolation & purification ; Female ; Gynecologic cancer ; Healthy Volunteers ; Human papillomaviruses ; Humans ; Lactobacillus - genetics ; Lactobacillus - isolation & purification ; Life Sciences & Biomedicine ; Microbiome ; Microbiota - genetics ; Middle Aged ; Obstetrics & Gynecology ; Oncology ; Phylogeny ; Porphyromonas - genetics ; Porphyromonas - isolation & purification ; Prevotella - genetics ; Prevotella - isolation & purification ; RNA, Ribosomal, 16S - genetics ; Science & Technology ; Squamous Intraepithelial Lesions of the Cervix - diagnosis ; Squamous Intraepithelial Lesions of the Cervix - microbiology ; Squamous Intraepithelial Lesions of the Cervix - pathology ; Uterine Cervical Neoplasms - diagnosis ; Uterine Cervical Neoplasms - microbiology ; Uterine Cervical Neoplasms - pathology ; Young Adult]]></subject><ispartof>Gynecologic oncology, 2021-11, Vol.163 (2), p.348-357</ispartof><rights>2018</rights><rights>Copyright © 2018. Published by Elsevier Inc.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>true</woscitedreferencessubscribed><woscitedreferencescount>34</woscitedreferencescount><woscitedreferencesoriginalsourcerecordid>wos000714728500020</woscitedreferencesoriginalsourcerecordid><citedby>FETCH-LOGICAL-c454t-974c887843de93c604369d6fdb1f251041dad5fc84d3b9bd723caadf0c51680c3</citedby><cites>FETCH-LOGICAL-c454t-974c887843de93c604369d6fdb1f251041dad5fc84d3b9bd723caadf0c51680c3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.ygyno.2021.08.016$$EHTML$$P50$$Gelsevier$$Hfree_for_read</linktohtml><link.rule.ids>315,781,785,3551,27929,27930,39263,46000</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/34503848$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Wu, Sikao</creatorcontrib><creatorcontrib>Ding, Xuewen</creatorcontrib><creatorcontrib>Kong, Ying</creatorcontrib><creatorcontrib>Acharya, Sanam</creatorcontrib><creatorcontrib>Wu, Huaqian</creatorcontrib><creatorcontrib>Huang, Chunning</creatorcontrib><creatorcontrib>Liang, Yuanyuan</creatorcontrib><creatorcontrib>Nong, Xianxian</creatorcontrib><creatorcontrib>Chen, Hong</creatorcontrib><title>The feature of cervical microbiota associated with the progression of cervical cancer among reproductive females</title><title>Gynecologic oncology</title><addtitle>GYNECOL ONCOL</addtitle><addtitle>Gynecol Oncol</addtitle><description>The aim of this study was to characterize cervical microbiome feature of reproductive-age women in the progression of squamous intraepithelial lesions (SIL) to cervical cancer.
We characterized the 16S rDNA cervical mucus microbiome in 94 participants (age from 18 to 52), including 13 cervical cancer (CA), 31 high-grade SIL (HSIL), 10 low-grade SIL (LSIL), 12 HPV-infected (NH) patients and 28 healthy controls (NN). Alpha (within sample) diversity was examined by Shannon and Simpson index, while Beta (between sample) diversity by principle coordinate analysis (PCoA) of weighted Unifrac distances. Relative abundance of microbial taxa was compared using Linear Discriminant Analysis Effect Size (LEfSe). Co-occurrence analysis was performed to identify correlation among marker genera, and Phylogenetic investigation of communities by reconstruction of unobserved states (PICRUSt) to explore functional features and pathways of cervical microbiota.
Alpha diversity(p < 0.05) was higher in severer cervical pathology with lower relative abundance of Lactobacillus as well as higher of anaerobes. Beta diversity (p < 0.01) was significantly different. Marker genera were identified including Porphyromonas, Prevotella and Campylobacter of CA and Sneathia of HSIL. The correlation of differential functional pathways with Prevotella was opposite to that with Lactobacillus.
Our study suggests differences in cervical microbiota diversity and relative abundance of reproductive-age females in different stages of cervical carcinogenesis. Marker genera might participate in the lesion progression and will be helpful for diagnosis, prevention and treatment. These findings may lead the way to further study of the cervical microbiome in development of cervical cancer.
•Cervical species diversity was higher in severer cervical intraepithelial neoplasia of reproductive-age women.•women with severer cervical pathology had more complex composition of cervical microbiota and more anaerobic bacteria.•Porphyromonas and Campylobacter were newfound marker genera of cervical cancer.•The correlation of differential functional pathways with Prevotella was opposite to that with Lactobacillus.•Marker genera in the lesion progression will be helpful for diagnosis, prevention and treatment.</description><subject>16S rRNA</subject><subject>Adult</subject><subject>Campylobacter - genetics</subject><subject>Campylobacter - isolation & purification</subject><subject>Case-Control Studies</subject><subject>Cervical cancer</subject><subject>Cervical microbiota</subject><subject>Cervix Uteri - microbiology</subject><subject>Cervix Uteri - pathology</subject><subject>Disease Progression</subject><subject>DNA, Bacterial - isolation & purification</subject><subject>Female</subject><subject>Gynecologic cancer</subject><subject>Healthy Volunteers</subject><subject>Human papillomaviruses</subject><subject>Humans</subject><subject>Lactobacillus - genetics</subject><subject>Lactobacillus - isolation & purification</subject><subject>Life Sciences & Biomedicine</subject><subject>Microbiome</subject><subject>Microbiota - genetics</subject><subject>Middle Aged</subject><subject>Obstetrics & Gynecology</subject><subject>Oncology</subject><subject>Phylogeny</subject><subject>Porphyromonas - genetics</subject><subject>Porphyromonas - isolation & purification</subject><subject>Prevotella - genetics</subject><subject>Prevotella - isolation & purification</subject><subject>RNA, Ribosomal, 16S - genetics</subject><subject>Science & Technology</subject><subject>Squamous Intraepithelial Lesions of the Cervix - diagnosis</subject><subject>Squamous Intraepithelial Lesions of the Cervix - microbiology</subject><subject>Squamous Intraepithelial Lesions of the Cervix - pathology</subject><subject>Uterine Cervical Neoplasms - diagnosis</subject><subject>Uterine Cervical Neoplasms - microbiology</subject><subject>Uterine Cervical Neoplasms - pathology</subject><subject>Young Adult</subject><issn>0090-8258</issn><issn>1095-6859</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2021</creationdate><recordtype>article</recordtype><sourceid>HGBXW</sourceid><sourceid>EIF</sourceid><recordid>eNqNkcFq3DAQhkVpaTZpn6BQfCwUuyNLsuVDD2Vpm0Cgl_QsZGm80WJbW0nesG8fbXcb6CXkpGH4Po3mFyEfKFQUaPNlWx02h9lXNdS0Alnl3iuyotCJspGie01WAB2UshbyglzGuAUABrR-Sy4YF8Aklyuyu7vHYkCdloCFHwqDYe-MHovJmeB755MudIzeOJ3QFg8u3RcpK7vgNwFjdH7-TzN6znWhJz9vioAZs4tJbn8cMukR4zvyZtBjxPfn84r8_vH9bn1d3v76ebP-dlsaLngqu5YbKVvJmcWOmQY4azrbDLanQy0ocGq1FYOR3LK-621bM6O1HcAI2kgw7Ip8Ot2bn_BnwZjU5KLBcdQz-iWqWrS0ow20XUbZCc0bxxhwULvgJh0OioI6Rq226m_U6hi1AqlyL1sfzwOWfkL75PzLNgPyBDxg74doHOZonrD8GS3lbS1FrmpYu6RTDnPtlzll9fPL1Ux_PdGY89w7DOpsWBfQJGW9e3aTR-i2s9E</recordid><startdate>202111</startdate><enddate>202111</enddate><creator>Wu, Sikao</creator><creator>Ding, Xuewen</creator><creator>Kong, Ying</creator><creator>Acharya, Sanam</creator><creator>Wu, Huaqian</creator><creator>Huang, Chunning</creator><creator>Liang, Yuanyuan</creator><creator>Nong, Xianxian</creator><creator>Chen, Hong</creator><general>Elsevier Inc</general><general>Elsevier</general><scope>6I.</scope><scope>AAFTH</scope><scope>BLEPL</scope><scope>DTL</scope><scope>HGBXW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>202111</creationdate><title>The feature of cervical microbiota associated with the progression of cervical cancer among reproductive females</title><author>Wu, Sikao ; Ding, Xuewen ; Kong, Ying ; Acharya, Sanam ; Wu, Huaqian ; Huang, Chunning ; Liang, Yuanyuan ; Nong, Xianxian ; Chen, Hong</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c454t-974c887843de93c604369d6fdb1f251041dad5fc84d3b9bd723caadf0c51680c3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2021</creationdate><topic>16S rRNA</topic><topic>Adult</topic><topic>Campylobacter - genetics</topic><topic>Campylobacter - isolation & purification</topic><topic>Case-Control Studies</topic><topic>Cervical cancer</topic><topic>Cervical microbiota</topic><topic>Cervix Uteri - microbiology</topic><topic>Cervix Uteri - pathology</topic><topic>Disease Progression</topic><topic>DNA, Bacterial - isolation & purification</topic><topic>Female</topic><topic>Gynecologic cancer</topic><topic>Healthy Volunteers</topic><topic>Human papillomaviruses</topic><topic>Humans</topic><topic>Lactobacillus - genetics</topic><topic>Lactobacillus - isolation & purification</topic><topic>Life Sciences & Biomedicine</topic><topic>Microbiome</topic><topic>Microbiota - genetics</topic><topic>Middle Aged</topic><topic>Obstetrics & Gynecology</topic><topic>Oncology</topic><topic>Phylogeny</topic><topic>Porphyromonas - genetics</topic><topic>Porphyromonas - isolation & purification</topic><topic>Prevotella - genetics</topic><topic>Prevotella - isolation & purification</topic><topic>RNA, Ribosomal, 16S - genetics</topic><topic>Science & Technology</topic><topic>Squamous Intraepithelial Lesions of the Cervix - diagnosis</topic><topic>Squamous Intraepithelial Lesions of the Cervix - microbiology</topic><topic>Squamous Intraepithelial Lesions of the Cervix - pathology</topic><topic>Uterine Cervical Neoplasms - diagnosis</topic><topic>Uterine Cervical Neoplasms - microbiology</topic><topic>Uterine Cervical Neoplasms - pathology</topic><topic>Young Adult</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Wu, Sikao</creatorcontrib><creatorcontrib>Ding, Xuewen</creatorcontrib><creatorcontrib>Kong, Ying</creatorcontrib><creatorcontrib>Acharya, Sanam</creatorcontrib><creatorcontrib>Wu, Huaqian</creatorcontrib><creatorcontrib>Huang, Chunning</creatorcontrib><creatorcontrib>Liang, Yuanyuan</creatorcontrib><creatorcontrib>Nong, Xianxian</creatorcontrib><creatorcontrib>Chen, Hong</creatorcontrib><collection>ScienceDirect Open Access Titles</collection><collection>Elsevier:ScienceDirect:Open Access</collection><collection>Web of Science Core Collection</collection><collection>Science Citation Index Expanded</collection><collection>Web of Science - Science Citation Index Expanded - 2021</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Gynecologic oncology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Wu, Sikao</au><au>Ding, Xuewen</au><au>Kong, Ying</au><au>Acharya, Sanam</au><au>Wu, Huaqian</au><au>Huang, Chunning</au><au>Liang, Yuanyuan</au><au>Nong, Xianxian</au><au>Chen, Hong</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>The feature of cervical microbiota associated with the progression of cervical cancer among reproductive females</atitle><jtitle>Gynecologic oncology</jtitle><stitle>GYNECOL ONCOL</stitle><addtitle>Gynecol Oncol</addtitle><date>2021-11</date><risdate>2021</risdate><volume>163</volume><issue>2</issue><spage>348</spage><epage>357</epage><pages>348-357</pages><issn>0090-8258</issn><eissn>1095-6859</eissn><abstract>The aim of this study was to characterize cervical microbiome feature of reproductive-age women in the progression of squamous intraepithelial lesions (SIL) to cervical cancer.
We characterized the 16S rDNA cervical mucus microbiome in 94 participants (age from 18 to 52), including 13 cervical cancer (CA), 31 high-grade SIL (HSIL), 10 low-grade SIL (LSIL), 12 HPV-infected (NH) patients and 28 healthy controls (NN). Alpha (within sample) diversity was examined by Shannon and Simpson index, while Beta (between sample) diversity by principle coordinate analysis (PCoA) of weighted Unifrac distances. Relative abundance of microbial taxa was compared using Linear Discriminant Analysis Effect Size (LEfSe). Co-occurrence analysis was performed to identify correlation among marker genera, and Phylogenetic investigation of communities by reconstruction of unobserved states (PICRUSt) to explore functional features and pathways of cervical microbiota.
Alpha diversity(p < 0.05) was higher in severer cervical pathology with lower relative abundance of Lactobacillus as well as higher of anaerobes. Beta diversity (p < 0.01) was significantly different. Marker genera were identified including Porphyromonas, Prevotella and Campylobacter of CA and Sneathia of HSIL. The correlation of differential functional pathways with Prevotella was opposite to that with Lactobacillus.
Our study suggests differences in cervical microbiota diversity and relative abundance of reproductive-age females in different stages of cervical carcinogenesis. Marker genera might participate in the lesion progression and will be helpful for diagnosis, prevention and treatment. These findings may lead the way to further study of the cervical microbiome in development of cervical cancer.
•Cervical species diversity was higher in severer cervical intraepithelial neoplasia of reproductive-age women.•women with severer cervical pathology had more complex composition of cervical microbiota and more anaerobic bacteria.•Porphyromonas and Campylobacter were newfound marker genera of cervical cancer.•The correlation of differential functional pathways with Prevotella was opposite to that with Lactobacillus.•Marker genera in the lesion progression will be helpful for diagnosis, prevention and treatment.</abstract><cop>SAN DIEGO</cop><pub>Elsevier Inc</pub><pmid>34503848</pmid><doi>10.1016/j.ygyno.2021.08.016</doi><tpages>10</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | 16S rRNA Adult Campylobacter - genetics Campylobacter - isolation & purification Case-Control Studies Cervical cancer Cervical microbiota Cervix Uteri - microbiology Cervix Uteri - pathology Disease Progression DNA, Bacterial - isolation & purification Female Gynecologic cancer Healthy Volunteers Human papillomaviruses Humans Lactobacillus - genetics Lactobacillus - isolation & purification Life Sciences & Biomedicine Microbiome Microbiota - genetics Middle Aged Obstetrics & Gynecology Oncology Phylogeny Porphyromonas - genetics Porphyromonas - isolation & purification Prevotella - genetics Prevotella - isolation & purification RNA, Ribosomal, 16S - genetics Science & Technology Squamous Intraepithelial Lesions of the Cervix - diagnosis Squamous Intraepithelial Lesions of the Cervix - microbiology Squamous Intraepithelial Lesions of the Cervix - pathology Uterine Cervical Neoplasms - diagnosis Uterine Cervical Neoplasms - microbiology Uterine Cervical Neoplasms - pathology Young Adult |
| title | The feature of cervical microbiota associated with the progression of cervical cancer among reproductive females |
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