Incorporation of proline analogs into recombinant proteins expressed in Escherichia coli

Proline residues are unique in the extent to which they constrain the conformational space available to the protein backbone. Because the conformational preferences of proline cannot be recapitulated by any of the other proteinogenic amino acids, standard mutagenesis approaches that seek to introduc...

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Veröffentlicht in:	Methods in enzymology 2021-01, Vol.656, p.545-571
Hauptverfasser:	Breunig, Stephanie L., Tirrell, David A.
Format:	Artikel
Sprache:	eng
Schlagworte:	Amino Acids Biochemical Research Methods Biochemistry & Molecular Biology Biotechnology & Applied Microbiology Chemistry Chemistry, Organic Escherichia coli - genetics Life Sciences & Biomedicine Molecular Conformation Physical Sciences Proline - genetics Protein Conformation Recombinant Proteins - genetics Science & Technology
Online-Zugang:	Volltext
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