Effects of small‐molecule compounds on fibroblast properties in golden snub‐nosed monkey (Rhinopithecus roxellana)
Background Golden snub‐nosed monkey (Rhinopithecus roxellana) is an endangered primate species, whose molecular material for conservation purposes has not yet been maintained. Although small‐molecule compounds (SMCs) have been reported to improve induced pluripotent stem cells (iPSCs), their efficie...
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| Veröffentlicht in: | Journal of medical primatology 2021-12, Vol.50 (6), p.323-331 |
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| container_title | Journal of medical primatology |
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| creator | Wang, Juanjuan Liu, Xin Yang, Jing Guo, Hanxing Li, Jingjing Huo, Lihui Zhao, Haitao Wang, Xiaowei Yan, Xingrong Li, Baoguo sun, Yu |
| description | Background
Golden snub‐nosed monkey (Rhinopithecus roxellana) is an endangered primate species, whose molecular material for conservation purposes has not yet been maintained. Although small‐molecule compounds (SMCs) have been reported to improve induced pluripotent stem cells (iPSCs), their efficiency in the interspecies‐transferred nucleus is still unknown.
Methods
We thus used the fibroblasts from the golden snub‐nosed monkey treated with SMC as donor cells, injected into the enucleated oocytes of goats, to test such efficiency. Gene expression profiles in the cell‐constructed embryos with and without SMCs were compared by qPCR.
Results
The results show that cell morphology undergoes remarkable changes (volume is smaller than normal cells, and many black spots in the cytoplasm were found); pluripotent genes (Oct4, Sox2, and Nanog) significantly increased with SMC treatment.
Conclusions
This study demonstrates that SMCs alter the properties of donor cells and promote the expression of pluripotent genes in hybrid embryos. |
| doi_str_mv | 10.1111/jmp.12549 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_webof</sourceid><recordid>TN_cdi_webofscience_primary_000708276600001</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>2583445360</sourcerecordid><originalsourceid>FETCH-LOGICAL-c3309-ed2e26ac43d1c806369927f47bd102a7404f30c3dbbd29132e89e8c219d5147e3</originalsourceid><addsrcrecordid>eNqNkctu1TAQhi0EoofCgjewxKYVSju-xImX6KjcVARCsI4cZ0x9cOwQJ4Wz4xF4Rp4E01OxQEJiNrOY7x_90kfIYwZnrMz5bpzOGK-lvkM2TAFUoFp5l2wAZFPxWtVH5EHOOwAQUsv75EhIpSRX7YZcXziHdsk0OZpHE8LP7z_GFNCuAalN45TWOJRrpM73c-qDyQud5jThvHjM1Ef6KYUBI81x7Us4powDHVP8jHt68v7KxzT55ao8zHRO3zAEE83pQ3LPmZDx0e0-Jh-fX3zYvqwu3754tX12WVkhQFc4cOTKWCkGZltQQmnNGyebfmDATSNBOgFWDH0_cM0Ex1ZjaznTQ81kg-KYnBz-lspfVsxLN_psb0pgWnPH61ZIWQsFBX3yF7pL6xxLu0JpxbRuhCjU6YGyc8p5RtdNsx_NvO8YdL9ldEVGdyOjsE8P7Ffsk8vWY7T4hy82Gmh5o4owAFbo9v_prV_M4lPcFj1LiZ7fRn3A_b8bda_fvDtU-wW_f60K</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2596199733</pqid></control><display><type>article</type><title>Effects of small‐molecule compounds on fibroblast properties in golden snub‐nosed monkey (Rhinopithecus roxellana)</title><source>Access via Wiley Online Library</source><source>Web of Science - Science Citation Index Expanded - 2021<img src="https://exlibris-pub.s3.amazonaws.com/fromwos-v2.jpg" /></source><creator>Wang, Juanjuan ; Liu, Xin ; Yang, Jing ; Guo, Hanxing ; Li, Jingjing ; Huo, Lihui ; Zhao, Haitao ; Wang, Xiaowei ; Yan, Xingrong ; Li, Baoguo ; sun, Yu</creator><creatorcontrib>Wang, Juanjuan ; Liu, Xin ; Yang, Jing ; Guo, Hanxing ; Li, Jingjing ; Huo, Lihui ; Zhao, Haitao ; Wang, Xiaowei ; Yan, Xingrong ; Li, Baoguo ; sun, Yu</creatorcontrib><description>Background
Golden snub‐nosed monkey (Rhinopithecus roxellana) is an endangered primate species, whose molecular material for conservation purposes has not yet been maintained. Although small‐molecule compounds (SMCs) have been reported to improve induced pluripotent stem cells (iPSCs), their efficiency in the interspecies‐transferred nucleus is still unknown.
Methods
We thus used the fibroblasts from the golden snub‐nosed monkey treated with SMC as donor cells, injected into the enucleated oocytes of goats, to test such efficiency. Gene expression profiles in the cell‐constructed embryos with and without SMCs were compared by qPCR.
Results
The results show that cell morphology undergoes remarkable changes (volume is smaller than normal cells, and many black spots in the cytoplasm were found); pluripotent genes (Oct4, Sox2, and Nanog) significantly increased with SMC treatment.
Conclusions
This study demonstrates that SMCs alter the properties of donor cells and promote the expression of pluripotent genes in hybrid embryos.</description><identifier>ISSN: 0047-2565</identifier><identifier>EISSN: 1600-0684</identifier><identifier>DOI: 10.1111/jmp.12549</identifier><identifier>PMID: 34664268</identifier><language>eng</language><publisher>HOBOKEN: Wiley</publisher><subject>Cytology ; Cytoplasm ; embryo ; Embryos ; Endangered species ; Fibroblasts ; Gene expression ; goat ; iSCNT ; Life Sciences & Biomedicine ; Oct-4 protein ; Oocytes ; Pluripotency ; Rhinopithecus roxellana ; Science & Technology ; Sichuan golden snub‐nosed monkey ; small‐molecule compounds ; Stem cell transplantation ; Stem cells ; Veterinary Sciences ; Zoology</subject><ispartof>Journal of medical primatology, 2021-12, Vol.50 (6), p.323-331</ispartof><rights>2021 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd</rights><rights>Copyright © 2021 John Wiley & Sons A/S</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>true</woscitedreferencessubscribed><woscitedreferencescount>1</woscitedreferencescount><woscitedreferencesoriginalsourcerecordid>wos000708276600001</woscitedreferencesoriginalsourcerecordid><citedby>FETCH-LOGICAL-c3309-ed2e26ac43d1c806369927f47bd102a7404f30c3dbbd29132e89e8c219d5147e3</citedby><cites>FETCH-LOGICAL-c3309-ed2e26ac43d1c806369927f47bd102a7404f30c3dbbd29132e89e8c219d5147e3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1111%2Fjmp.12549$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1111%2Fjmp.12549$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>315,781,785,1418,27929,27930,39263,45579,45580</link.rule.ids></links><search><creatorcontrib>Wang, Juanjuan</creatorcontrib><creatorcontrib>Liu, Xin</creatorcontrib><creatorcontrib>Yang, Jing</creatorcontrib><creatorcontrib>Guo, Hanxing</creatorcontrib><creatorcontrib>Li, Jingjing</creatorcontrib><creatorcontrib>Huo, Lihui</creatorcontrib><creatorcontrib>Zhao, Haitao</creatorcontrib><creatorcontrib>Wang, Xiaowei</creatorcontrib><creatorcontrib>Yan, Xingrong</creatorcontrib><creatorcontrib>Li, Baoguo</creatorcontrib><creatorcontrib>sun, Yu</creatorcontrib><title>Effects of small‐molecule compounds on fibroblast properties in golden snub‐nosed monkey (Rhinopithecus roxellana)</title><title>Journal of medical primatology</title><addtitle>J MED PRIMATOL</addtitle><description>Background
Golden snub‐nosed monkey (Rhinopithecus roxellana) is an endangered primate species, whose molecular material for conservation purposes has not yet been maintained. Although small‐molecule compounds (SMCs) have been reported to improve induced pluripotent stem cells (iPSCs), their efficiency in the interspecies‐transferred nucleus is still unknown.
Methods
We thus used the fibroblasts from the golden snub‐nosed monkey treated with SMC as donor cells, injected into the enucleated oocytes of goats, to test such efficiency. Gene expression profiles in the cell‐constructed embryos with and without SMCs were compared by qPCR.
Results
The results show that cell morphology undergoes remarkable changes (volume is smaller than normal cells, and many black spots in the cytoplasm were found); pluripotent genes (Oct4, Sox2, and Nanog) significantly increased with SMC treatment.
Conclusions
This study demonstrates that SMCs alter the properties of donor cells and promote the expression of pluripotent genes in hybrid embryos.</description><subject>Cytology</subject><subject>Cytoplasm</subject><subject>embryo</subject><subject>Embryos</subject><subject>Endangered species</subject><subject>Fibroblasts</subject><subject>Gene expression</subject><subject>goat</subject><subject>iSCNT</subject><subject>Life Sciences & Biomedicine</subject><subject>Oct-4 protein</subject><subject>Oocytes</subject><subject>Pluripotency</subject><subject>Rhinopithecus roxellana</subject><subject>Science & Technology</subject><subject>Sichuan golden snub‐nosed monkey</subject><subject>small‐molecule compounds</subject><subject>Stem cell transplantation</subject><subject>Stem cells</subject><subject>Veterinary Sciences</subject><subject>Zoology</subject><issn>0047-2565</issn><issn>1600-0684</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2021</creationdate><recordtype>article</recordtype><sourceid>HGBXW</sourceid><recordid>eNqNkctu1TAQhi0EoofCgjewxKYVSju-xImX6KjcVARCsI4cZ0x9cOwQJ4Wz4xF4Rp4E01OxQEJiNrOY7x_90kfIYwZnrMz5bpzOGK-lvkM2TAFUoFp5l2wAZFPxWtVH5EHOOwAQUsv75EhIpSRX7YZcXziHdsk0OZpHE8LP7z_GFNCuAalN45TWOJRrpM73c-qDyQud5jThvHjM1Ef6KYUBI81x7Us4powDHVP8jHt68v7KxzT55ao8zHRO3zAEE83pQ3LPmZDx0e0-Jh-fX3zYvqwu3754tX12WVkhQFc4cOTKWCkGZltQQmnNGyebfmDATSNBOgFWDH0_cM0Ex1ZjaznTQ81kg-KYnBz-lspfVsxLN_psb0pgWnPH61ZIWQsFBX3yF7pL6xxLu0JpxbRuhCjU6YGyc8p5RtdNsx_NvO8YdL9ldEVGdyOjsE8P7Ffsk8vWY7T4hy82Gmh5o4owAFbo9v_prV_M4lPcFj1LiZ7fRn3A_b8bda_fvDtU-wW_f60K</recordid><startdate>202112</startdate><enddate>202112</enddate><creator>Wang, Juanjuan</creator><creator>Liu, Xin</creator><creator>Yang, Jing</creator><creator>Guo, Hanxing</creator><creator>Li, Jingjing</creator><creator>Huo, Lihui</creator><creator>Zhao, Haitao</creator><creator>Wang, Xiaowei</creator><creator>Yan, Xingrong</creator><creator>Li, Baoguo</creator><creator>sun, Yu</creator><general>Wiley</general><general>Wiley Subscription Services, Inc</general><scope>BLEPL</scope><scope>DTL</scope><scope>HGBXW</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QP</scope><scope>7TK</scope><scope>K9.</scope><scope>7X8</scope></search><sort><creationdate>202112</creationdate><title>Effects of small‐molecule compounds on fibroblast properties in golden snub‐nosed monkey (Rhinopithecus roxellana)</title><author>Wang, Juanjuan ; Liu, Xin ; Yang, Jing ; Guo, Hanxing ; Li, Jingjing ; Huo, Lihui ; Zhao, Haitao ; Wang, Xiaowei ; Yan, Xingrong ; Li, Baoguo ; sun, Yu</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c3309-ed2e26ac43d1c806369927f47bd102a7404f30c3dbbd29132e89e8c219d5147e3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2021</creationdate><topic>Cytology</topic><topic>Cytoplasm</topic><topic>embryo</topic><topic>Embryos</topic><topic>Endangered species</topic><topic>Fibroblasts</topic><topic>Gene expression</topic><topic>goat</topic><topic>iSCNT</topic><topic>Life Sciences & Biomedicine</topic><topic>Oct-4 protein</topic><topic>Oocytes</topic><topic>Pluripotency</topic><topic>Rhinopithecus roxellana</topic><topic>Science & Technology</topic><topic>Sichuan golden snub‐nosed monkey</topic><topic>small‐molecule compounds</topic><topic>Stem cell transplantation</topic><topic>Stem cells</topic><topic>Veterinary Sciences</topic><topic>Zoology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Wang, Juanjuan</creatorcontrib><creatorcontrib>Liu, Xin</creatorcontrib><creatorcontrib>Yang, Jing</creatorcontrib><creatorcontrib>Guo, Hanxing</creatorcontrib><creatorcontrib>Li, Jingjing</creatorcontrib><creatorcontrib>Huo, Lihui</creatorcontrib><creatorcontrib>Zhao, Haitao</creatorcontrib><creatorcontrib>Wang, Xiaowei</creatorcontrib><creatorcontrib>Yan, Xingrong</creatorcontrib><creatorcontrib>Li, Baoguo</creatorcontrib><creatorcontrib>sun, Yu</creatorcontrib><collection>Web of Science Core Collection</collection><collection>Science Citation Index Expanded</collection><collection>Web of Science - Science Citation Index Expanded - 2021</collection><collection>CrossRef</collection><collection>Calcium & Calcified Tissue Abstracts</collection><collection>Neurosciences Abstracts</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of medical primatology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Wang, Juanjuan</au><au>Liu, Xin</au><au>Yang, Jing</au><au>Guo, Hanxing</au><au>Li, Jingjing</au><au>Huo, Lihui</au><au>Zhao, Haitao</au><au>Wang, Xiaowei</au><au>Yan, Xingrong</au><au>Li, Baoguo</au><au>sun, Yu</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Effects of small‐molecule compounds on fibroblast properties in golden snub‐nosed monkey (Rhinopithecus roxellana)</atitle><jtitle>Journal of medical primatology</jtitle><stitle>J MED PRIMATOL</stitle><date>2021-12</date><risdate>2021</risdate><volume>50</volume><issue>6</issue><spage>323</spage><epage>331</epage><pages>323-331</pages><issn>0047-2565</issn><eissn>1600-0684</eissn><abstract>Background
Golden snub‐nosed monkey (Rhinopithecus roxellana) is an endangered primate species, whose molecular material for conservation purposes has not yet been maintained. Although small‐molecule compounds (SMCs) have been reported to improve induced pluripotent stem cells (iPSCs), their efficiency in the interspecies‐transferred nucleus is still unknown.
Methods
We thus used the fibroblasts from the golden snub‐nosed monkey treated with SMC as donor cells, injected into the enucleated oocytes of goats, to test such efficiency. Gene expression profiles in the cell‐constructed embryos with and without SMCs were compared by qPCR.
Results
The results show that cell morphology undergoes remarkable changes (volume is smaller than normal cells, and many black spots in the cytoplasm were found); pluripotent genes (Oct4, Sox2, and Nanog) significantly increased with SMC treatment.
Conclusions
This study demonstrates that SMCs alter the properties of donor cells and promote the expression of pluripotent genes in hybrid embryos.</abstract><cop>HOBOKEN</cop><pub>Wiley</pub><pmid>34664268</pmid><doi>10.1111/jmp.12549</doi></addata></record> |
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| subjects | Cytology Cytoplasm embryo Embryos Endangered species Fibroblasts Gene expression goat iSCNT Life Sciences & Biomedicine Oct-4 protein Oocytes Pluripotency Rhinopithecus roxellana Science & Technology Sichuan golden snub‐nosed monkey small‐molecule compounds Stem cell transplantation Stem cells Veterinary Sciences Zoology |
| title | Effects of small‐molecule compounds on fibroblast properties in golden snub‐nosed monkey (Rhinopithecus roxellana) |
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