Latest progress in the study of nanoparticle-based delivery of the CRISPR/Cas9 system

•CRISPR/Cas9 system has been used for gene editing in basic research and clinical applications.•A number of CRISPR/Cas9 vehicles including lipofectamine, viruses, and nanoparticles exist.•Different CRISPR/Cas9 delivery systems face numerous challenges during their applications.•We focus on the nanop...

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Veröffentlicht in:Methods (San Diego, Calif.) Calif.), 2021-10, Vol.194, p.48-55
Hauptverfasser: Yu, Mingyu, Liu, Xiaowen, Cheng, Hongbo, Kuang, Longhao, Zhang, Shaochong, Yan, Xiaohe
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Sprache:eng
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Zusammenfassung:•CRISPR/Cas9 system has been used for gene editing in basic research and clinical applications.•A number of CRISPR/Cas9 vehicles including lipofectamine, viruses, and nanoparticles exist.•Different CRISPR/Cas9 delivery systems face numerous challenges during their applications.•We focus on the nanoparticle delivery of CRISPR/Cas9 due to its safety, simplicity and flexibility. The CRISPR/Cas9 system has been harnessed to cleave a targeted DNA fragment via its Cas nuclease activity under the direction of guide RNA for rendering gene insertions, deletions, and point mutations in basic research and clinical applications. There are a number of vehicles, including lipofectamine, viruses, and nanoparticles, that can deliver the CRISPR/Cas9 system, but all these methods face numerous challenges during their application in life science contexts. Here, we focus on the delivery of CRISPR/Cas9 via nanoparticles because this method has shown great advantages in terms of safety, simplicity and flexibility.
ISSN:1046-2023
1095-9130
DOI:10.1016/j.ymeth.2021.06.004