A Novel Fat Making Strategy With Adipose-Derived Progenitor Cell-Enriched Fat Improves Fat Graft Survival
Abstract Background A low survival rate is one of the main challenges in fat grafting. Objectives This study aimed to evaluate whether microfat obtained by a novel strategy promoted the survival and retention of fat grafts. Methods A 5-mm-diameter blunt tip cannula with large side holes (~30 mm2/hol...
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| Veröffentlicht in: | Aesthetic surgery journal 2021-08, Vol.41 (9), p.1228-1236 |
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| container_title | Aesthetic surgery journal |
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| creator | Fan, Pengju Fang, Man Li, Jingjing Solari, Mario G Wu, Dingyu Tan, Wuyuan Wang, Yongjie Yang, Xinghua Lei, Shaorong |
| description | Abstract
Background
A low survival rate is one of the main challenges in fat grafting.
Objectives
This study aimed to evaluate whether microfat obtained by a novel strategy promoted the survival and retention of fat grafts.
Methods
A 5-mm-diameter blunt tip cannula with large side holes (~30 mm2/hole) was used to obtain macrofat. A novel strategy based on a newly invented extracorporeal cutting device was then used to cut the macrofat into microfat, which was named adipose-derived progenitor cell enrichment fat (AER fat); Coleman fat was used as the control. Aliquots (0.5 mL) of both types of fat were transplanted into 10 nude mice and analyzed 10 weeks later. Western blotting, flow cytometry, and immunofluorescence were performed to assess the AER fat characteristics and underlying mechanisms.
Results
The retention rate of fat grafts in AER fat–treated animals was significantly higher than that in the Coleman group (mean [standard deviation] 54.6% [13%] vs 34.8% [9%]; P |
| doi_str_mv | 10.1093/asj/sjab216 |
| format | Article |
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Background
A low survival rate is one of the main challenges in fat grafting.
Objectives
This study aimed to evaluate whether microfat obtained by a novel strategy promoted the survival and retention of fat grafts.
Methods
A 5-mm-diameter blunt tip cannula with large side holes (~30 mm2/hole) was used to obtain macrofat. A novel strategy based on a newly invented extracorporeal cutting device was then used to cut the macrofat into microfat, which was named adipose-derived progenitor cell enrichment fat (AER fat); Coleman fat was used as the control. Aliquots (0.5 mL) of both types of fat were transplanted into 10 nude mice and analyzed 10 weeks later. Western blotting, flow cytometry, and immunofluorescence were performed to assess the AER fat characteristics and underlying mechanisms.
Results
The retention rate of fat grafts in AER fat–treated animals was significantly higher than that in the Coleman group (mean [standard deviation] 54.6% [13%] vs 34.8% [9%]; P < 0.05) after 10 weeks. AER fat contained more dipeptidyl peptidase-4–expressing progenitor cells (3.3 [0.61] × 103 vs 2.0 [0.46] × 103 cells/mL; P < 0.05), adipose-derived plastic-adherent cells (6.0 [1.10] × 104 vs 2.6 [0.17] × 104 cells/mL; P < 0.001), and viable adipocytes than Coleman fat. Moreover, histologic analysis showed that AER fat grafts had better histologic structure and higher capillary density.
Conclusions
AER fat transplantation is a potential strategy to improve the survival and long-term retention of fat grafts. AER fat contained more dipeptidyl peptidase-4–expressing progenitor cells.</description><identifier>ISSN: 1090-820X</identifier><identifier>EISSN: 1527-330X</identifier><identifier>DOI: 10.1093/asj/sjab216</identifier><identifier>PMID: 34387330</identifier><language>eng</language><publisher>US: Oxford University Press</publisher><subject>Adipocytes ; Adipose Tissue ; Animals ; Graft Survival ; Mice ; Mice, Nude ; Stem Cells</subject><ispartof>Aesthetic surgery journal, 2021-08, Vol.41 (9), p.1228-1236</ispartof><rights>2021 The Aesthetic Society. Reprints and permission: journals.permissions@oup.com 2021</rights><rights>2021 The Aesthetic Society. Reprints and permission: journals.permissions@oup.com.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c320t-3f30412ec2c0529bd2d74d22c73cc36c7309274b7cefc739303c4305d6eedf9e3</citedby><cites>FETCH-LOGICAL-c320t-3f30412ec2c0529bd2d74d22c73cc36c7309274b7cefc739303c4305d6eedf9e3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,1584,27924,27925</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/34387330$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Fan, Pengju</creatorcontrib><creatorcontrib>Fang, Man</creatorcontrib><creatorcontrib>Li, Jingjing</creatorcontrib><creatorcontrib>Solari, Mario G</creatorcontrib><creatorcontrib>Wu, Dingyu</creatorcontrib><creatorcontrib>Tan, Wuyuan</creatorcontrib><creatorcontrib>Wang, Yongjie</creatorcontrib><creatorcontrib>Yang, Xinghua</creatorcontrib><creatorcontrib>Lei, Shaorong</creatorcontrib><title>A Novel Fat Making Strategy With Adipose-Derived Progenitor Cell-Enriched Fat Improves Fat Graft Survival</title><title>Aesthetic surgery journal</title><addtitle>Aesthet Surg J</addtitle><description>Abstract
Background
A low survival rate is one of the main challenges in fat grafting.
Objectives
This study aimed to evaluate whether microfat obtained by a novel strategy promoted the survival and retention of fat grafts.
Methods
A 5-mm-diameter blunt tip cannula with large side holes (~30 mm2/hole) was used to obtain macrofat. A novel strategy based on a newly invented extracorporeal cutting device was then used to cut the macrofat into microfat, which was named adipose-derived progenitor cell enrichment fat (AER fat); Coleman fat was used as the control. Aliquots (0.5 mL) of both types of fat were transplanted into 10 nude mice and analyzed 10 weeks later. Western blotting, flow cytometry, and immunofluorescence were performed to assess the AER fat characteristics and underlying mechanisms.
Results
The retention rate of fat grafts in AER fat–treated animals was significantly higher than that in the Coleman group (mean [standard deviation] 54.6% [13%] vs 34.8% [9%]; P < 0.05) after 10 weeks. AER fat contained more dipeptidyl peptidase-4–expressing progenitor cells (3.3 [0.61] × 103 vs 2.0 [0.46] × 103 cells/mL; P < 0.05), adipose-derived plastic-adherent cells (6.0 [1.10] × 104 vs 2.6 [0.17] × 104 cells/mL; P < 0.001), and viable adipocytes than Coleman fat. Moreover, histologic analysis showed that AER fat grafts had better histologic structure and higher capillary density.
Conclusions
AER fat transplantation is a potential strategy to improve the survival and long-term retention of fat grafts. AER fat contained more dipeptidyl peptidase-4–expressing progenitor cells.</description><subject>Adipocytes</subject><subject>Adipose Tissue</subject><subject>Animals</subject><subject>Graft Survival</subject><subject>Mice</subject><subject>Mice, Nude</subject><subject>Stem Cells</subject><issn>1090-820X</issn><issn>1527-330X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2021</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kEtLw0AURgdRrFZX7mVWIkjsPPJcltrWQn1AFbsLk8lNOzUvZ5JA_71TW126-u7lHg6XD6ErSu4pifhAmM3AbETCqH-EzqjHAodzsjy2M4mIEzKy7KFzYzaEWNx3T1GPuzwMLHSG1BA_Vx3keCIa_CQ-VbnCi0aLBlZb_KGaNR6mqq4MOA-gVQcpftXVCkrVVBqPIM-dcamVXNvDzjAram115meZapE1eNHqTnUiv0AnmcgNXB6yj94n47fRozN_mc5Gw7kjOSONwzNOXMpAMkk8FiUpSwM3ZUwGXEru2yARC9wkkJDZJeKES5cTL_UB0iwC3ke3e6_95KsF08SFMtJ-KkqoWhMzz6du6IYhtejdHpW6MkZDFtdaFUJvY0riXbex7TY-dGvp64O4TQpI_9jfMi1wsweqtv7X9A1bJ4LW</recordid><startdate>20210813</startdate><enddate>20210813</enddate><creator>Fan, Pengju</creator><creator>Fang, Man</creator><creator>Li, Jingjing</creator><creator>Solari, Mario G</creator><creator>Wu, Dingyu</creator><creator>Tan, Wuyuan</creator><creator>Wang, Yongjie</creator><creator>Yang, Xinghua</creator><creator>Lei, Shaorong</creator><general>Oxford University Press</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20210813</creationdate><title>A Novel Fat Making Strategy With Adipose-Derived Progenitor Cell-Enriched Fat Improves Fat Graft Survival</title><author>Fan, Pengju ; Fang, Man ; Li, Jingjing ; Solari, Mario G ; Wu, Dingyu ; Tan, Wuyuan ; Wang, Yongjie ; Yang, Xinghua ; Lei, Shaorong</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c320t-3f30412ec2c0529bd2d74d22c73cc36c7309274b7cefc739303c4305d6eedf9e3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2021</creationdate><topic>Adipocytes</topic><topic>Adipose Tissue</topic><topic>Animals</topic><topic>Graft Survival</topic><topic>Mice</topic><topic>Mice, Nude</topic><topic>Stem Cells</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Fan, Pengju</creatorcontrib><creatorcontrib>Fang, Man</creatorcontrib><creatorcontrib>Li, Jingjing</creatorcontrib><creatorcontrib>Solari, Mario G</creatorcontrib><creatorcontrib>Wu, Dingyu</creatorcontrib><creatorcontrib>Tan, Wuyuan</creatorcontrib><creatorcontrib>Wang, Yongjie</creatorcontrib><creatorcontrib>Yang, Xinghua</creatorcontrib><creatorcontrib>Lei, Shaorong</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Aesthetic surgery journal</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Fan, Pengju</au><au>Fang, Man</au><au>Li, Jingjing</au><au>Solari, Mario G</au><au>Wu, Dingyu</au><au>Tan, Wuyuan</au><au>Wang, Yongjie</au><au>Yang, Xinghua</au><au>Lei, Shaorong</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>A Novel Fat Making Strategy With Adipose-Derived Progenitor Cell-Enriched Fat Improves Fat Graft Survival</atitle><jtitle>Aesthetic surgery journal</jtitle><addtitle>Aesthet Surg J</addtitle><date>2021-08-13</date><risdate>2021</risdate><volume>41</volume><issue>9</issue><spage>1228</spage><epage>1236</epage><pages>1228-1236</pages><issn>1090-820X</issn><eissn>1527-330X</eissn><abstract>Abstract
Background
A low survival rate is one of the main challenges in fat grafting.
Objectives
This study aimed to evaluate whether microfat obtained by a novel strategy promoted the survival and retention of fat grafts.
Methods
A 5-mm-diameter blunt tip cannula with large side holes (~30 mm2/hole) was used to obtain macrofat. A novel strategy based on a newly invented extracorporeal cutting device was then used to cut the macrofat into microfat, which was named adipose-derived progenitor cell enrichment fat (AER fat); Coleman fat was used as the control. Aliquots (0.5 mL) of both types of fat were transplanted into 10 nude mice and analyzed 10 weeks later. Western blotting, flow cytometry, and immunofluorescence were performed to assess the AER fat characteristics and underlying mechanisms.
Results
The retention rate of fat grafts in AER fat–treated animals was significantly higher than that in the Coleman group (mean [standard deviation] 54.6% [13%] vs 34.8% [9%]; P < 0.05) after 10 weeks. AER fat contained more dipeptidyl peptidase-4–expressing progenitor cells (3.3 [0.61] × 103 vs 2.0 [0.46] × 103 cells/mL; P < 0.05), adipose-derived plastic-adherent cells (6.0 [1.10] × 104 vs 2.6 [0.17] × 104 cells/mL; P < 0.001), and viable adipocytes than Coleman fat. Moreover, histologic analysis showed that AER fat grafts had better histologic structure and higher capillary density.
Conclusions
AER fat transplantation is a potential strategy to improve the survival and long-term retention of fat grafts. AER fat contained more dipeptidyl peptidase-4–expressing progenitor cells.</abstract><cop>US</cop><pub>Oxford University Press</pub><pmid>34387330</pmid><doi>10.1093/asj/sjab216</doi><tpages>9</tpages></addata></record> |
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| source | MEDLINE; Oxford University Press Journals All Titles (1996-Current); Alma/SFX Local Collection |
| subjects | Adipocytes Adipose Tissue Animals Graft Survival Mice Mice, Nude Stem Cells |
| title | A Novel Fat Making Strategy With Adipose-Derived Progenitor Cell-Enriched Fat Improves Fat Graft Survival |
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