Unified platform for genetic and serological detection of COVID-19 with single-molecule technology

The COVID-19 pandemic raises the need for diverse diagnostic approaches to rapidly detect different stages of viral infection. The flexible and quantitative nature of single-molecule imaging technology renders it optimal for development of new diagnostic tools. Here we present a proof-of-concept for...

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Veröffentlicht in:	PloS one 2021-07, Vol.16 (7), p.e0255096-e0255096, Article 0255096
Hauptverfasser:	Furth, Noa, Shilo, Shay, Cohen, Niv, Erez, Nir, Fedyuk, Vadim, Schrager, Alexander M., Weinberger, Adina, Dror, Amiel A., Zigron, Asaf, Shehadeh, Mona, Sela, Eyal, Srouji, Samer, Amit, Sharon, Levy, Itzchak, Segal, Eran, Dahan, Rony, Jones, Dan, Douek, Daniel C., Shema, Efrat
Format:	Artikel
Sprache:	eng
Schlagworte:	Analysis Antibodies Biology and Life Sciences Coronaviruses COVID-19 COVID-19 vaccines Datasets Genomes Human subjects Immune response Immunoglobulin G Immunoglobulin M Immunology Laboratories Medicine Medicine and Health Sciences Multidisciplinary Sciences Otolaryngology Pandemics Research and Analysis Methods RNA Science & Technology Science & Technology - Other Topics Serology Severe acute respiratory syndrome coronavirus 2 Technology Viral diseases Viral infections
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Beschreibung
Zusammenfassung:	The COVID-19 pandemic raises the need for diverse diagnostic approaches to rapidly detect different stages of viral infection. The flexible and quantitative nature of single-molecule imaging technology renders it optimal for development of new diagnostic tools. Here we present a proof-of-concept for a single-molecule based, enzyme-free assay for detection of SARS-CoV-2. The unified platform we developed allows direct detection of the viral genetic material from patients' samples, as well as their immune response consisting of IgG and IgM antibodies. Thus, it establishes a platform for diagnostics of COVID-19, which could also be adjusted to diagnose additional pathogens.
ISSN:	1932-6203 1932-6203
DOI:	10.1371/journal.pone.0255096