Probing an Ixodes ricinus salivary gland yeast surface display with tick-exposed human sera to identify novel candidates for an anti-tick vaccine

In Europe, Ixodes ricinus is the most important vector of human infectious diseases, most notably Lyme borreliosis and tick-borne encephalitis virus. Multiple non-natural hosts of I. ricinus have shown to develop immunity after repeated tick bites. Tick immunity has also been shown to impair B. burg...

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Veröffentlicht in:Scientific reports 2021-08, Vol.11 (1), p.15745-15745, Article 15745
Hauptverfasser: Trentelman, Jos J. A., Tomás-Cortázar, Julen, Knorr, Sarah, Barriales, Diego, Hajdusek, Ondrej, Sima, Radek, Ersoz, Jasmin I., Narasimhan, Sukanya, Fikrig, Erol, Nijhof, Ard M., Anguita, Juan, Hovius, Joppe W.
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container_title Scientific reports
container_volume 11
creator Trentelman, Jos J. A.
Tomás-Cortázar, Julen
Knorr, Sarah
Barriales, Diego
Hajdusek, Ondrej
Sima, Radek
Ersoz, Jasmin I.
Narasimhan, Sukanya
Fikrig, Erol
Nijhof, Ard M.
Anguita, Juan
Hovius, Joppe W.
description In Europe, Ixodes ricinus is the most important vector of human infectious diseases, most notably Lyme borreliosis and tick-borne encephalitis virus. Multiple non-natural hosts of I. ricinus have shown to develop immunity after repeated tick bites. Tick immunity has also been shown to impair B. burgdorferi transmission. Most interestingly, multiple tick bites reduced the likelihood of contracting Lyme borreliosis in humans. A vaccine that mimics tick immunity could therefore potentially prevent Lyme borreliosis in humans. A yeast surface display library (YSD) of nymphal I. ricinus salivary gland genes expressed at 24, 48 and 72 h into tick feeding was constructed and probed with antibodies from humans repeatedly bitten by ticks, identifying twelve immunoreactive tick salivary gland proteins (TSGPs). From these, three proteins were selected for vaccination studies. An exploratory vaccination study in cattle showed an anti-tick effect when all three antigens were combined. However, immunization of rabbits did not provide equivalent levels of protection. Our results show that YSD is a powerful tool to identify immunodominant antigens in humans exposed to tick bites, yet vaccination with the three selected TSGPs did not provide protection in the present form. Future efforts will focus on exploring the biological functions of these proteins, consider alternative systems for recombinant protein generation and vaccination platforms and assess the potential of the other identified immunogenic TSGPs.
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A vaccine that mimics tick immunity could therefore potentially prevent Lyme borreliosis in humans. A yeast surface display library (YSD) of nymphal I. ricinus salivary gland genes expressed at 24, 48 and 72 h into tick feeding was constructed and probed with antibodies from humans repeatedly bitten by ticks, identifying twelve immunoreactive tick salivary gland proteins (TSGPs). From these, three proteins were selected for vaccination studies. An exploratory vaccination study in cattle showed an anti-tick effect when all three antigens were combined. However, immunization of rabbits did not provide equivalent levels of protection. Our results show that YSD is a powerful tool to identify immunodominant antigens in humans exposed to tick bites, yet vaccination with the three selected TSGPs did not provide protection in the present form. 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subjects 631/154/555
631/250/590
692/699/255/1715
Antigens
Arachnids
Borreliosis
Encephalitis
Exocrine glands
Humanities and Social Sciences
Immunization
Immunogenicity
Infectious diseases
Insect bites
Ixodes ricinus
multidisciplinary
Multidisciplinary Sciences
Proteins
Salivary gland
Science
Science & Technology
Science & Technology - Other Topics
Science (multidisciplinary)
Tick-borne encephalitis
Vaccination
Vaccines
Yeasts
title Probing an Ixodes ricinus salivary gland yeast surface display with tick-exposed human sera to identify novel candidates for an anti-tick vaccine
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