Protective effect of glutathione S-transferase enzyme activity against aflatoxin B 1 in poultry species: relationship between glutathione S-transferase enzyme kinetic parameters, and resistance to aflatoxin B 1
Comparative studies designed to investigate the role of glutathione S-transferase (GST) activity on the enzyme catalyzed trapping of aflatoxin B -8,9-epoxide (AFBO) with glutathione, and the relationship with aflatoxin B (AFB ) resistance have not been conducted in poultry. Hepatic cytosolic fractio...
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| Veröffentlicht in: | Poultry science 2021-08, Vol.100 (8), p.101235 |
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| creator | Murcia, Hansen W Diaz, Gonzalo J |
| description | Comparative studies designed to investigate the role of glutathione S-transferase (GST) activity on the enzyme catalyzed trapping of aflatoxin B
-8,9-epoxide (AFBO) with glutathione, and the relationship with aflatoxin B
(AFB
) resistance have not been conducted in poultry. Hepatic cytosolic fractions of chickens, quail, turkeys and ducks were used to measure in vitro the enzymatic parameters maximal velocity (V
), Michaelis-Menten constant (K
) and intrinsic clearance (CL
) for GST activity. AFB
used ranged from 2.0 to 157.5 µM and the AFB
-GSH produced was identified and quantitated by HPLC. Significant differences were found in GST V
values, being the highest in chickens, followed by quail, ducks and turkeys. The K
values were also significantly different, with chickens < ducks < turkeys < quail. Chickens had the higher CL
value in contrast to ducks. Differences by sex showed that duck females had a higher CL
value than the turkey and quail, whereas duck males had a CL
close to that of turkey. The ratio "AFBO production /AFB
-GSH production" follows the order duck>turkey>quail>chicken, in agreement with the known poultry sensitivity. The extremely high "AFB
epoxidation activity/ GST activity" ratio observed in ducks might be the explanation for the development of hepatocellular carcinoma in this species. |
| doi_str_mv | 10.1016/j.psj.2021.101235 |
| format | Article |
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-8,9-epoxide (AFBO) with glutathione, and the relationship with aflatoxin B
(AFB
) resistance have not been conducted in poultry. Hepatic cytosolic fractions of chickens, quail, turkeys and ducks were used to measure in vitro the enzymatic parameters maximal velocity (V
), Michaelis-Menten constant (K
) and intrinsic clearance (CL
) for GST activity. AFB
used ranged from 2.0 to 157.5 µM and the AFB
-GSH produced was identified and quantitated by HPLC. Significant differences were found in GST V
values, being the highest in chickens, followed by quail, ducks and turkeys. The K
values were also significantly different, with chickens < ducks < turkeys < quail. Chickens had the higher CL
value in contrast to ducks. Differences by sex showed that duck females had a higher CL
value than the turkey and quail, whereas duck males had a CL
close to that of turkey. The ratio "AFBO production /AFB
-GSH production" follows the order duck>turkey>quail>chicken, in agreement with the known poultry sensitivity. The extremely high "AFB
epoxidation activity/ GST activity" ratio observed in ducks might be the explanation for the development of hepatocellular carcinoma in this species.</description><identifier>EISSN: 1525-3171</identifier><identifier>DOI: 10.1016/j.psj.2021.101235</identifier><identifier>PMID: 34214746</identifier><language>eng</language><publisher>England</publisher><subject>Aflatoxin B1 ; Animals ; Chickens ; Ducks ; Female ; Glutathione Transferase ; Liver ; Male ; Poultry ; Quail ; Turkeys</subject><ispartof>Poultry science, 2021-08, Vol.100 (8), p.101235</ispartof><rights>Copyright © 2021 The Authors. Published by Elsevier Inc. All rights reserved.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,864,27924,27925</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/34214746$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Murcia, Hansen W</creatorcontrib><creatorcontrib>Diaz, Gonzalo J</creatorcontrib><title>Protective effect of glutathione S-transferase enzyme activity against aflatoxin B 1 in poultry species: relationship between glutathione S-transferase enzyme kinetic parameters, and resistance to aflatoxin B 1</title><title>Poultry science</title><addtitle>Poult Sci</addtitle><description>Comparative studies designed to investigate the role of glutathione S-transferase (GST) activity on the enzyme catalyzed trapping of aflatoxin B
-8,9-epoxide (AFBO) with glutathione, and the relationship with aflatoxin B
(AFB
) resistance have not been conducted in poultry. Hepatic cytosolic fractions of chickens, quail, turkeys and ducks were used to measure in vitro the enzymatic parameters maximal velocity (V
), Michaelis-Menten constant (K
) and intrinsic clearance (CL
) for GST activity. AFB
used ranged from 2.0 to 157.5 µM and the AFB
-GSH produced was identified and quantitated by HPLC. Significant differences were found in GST V
values, being the highest in chickens, followed by quail, ducks and turkeys. The K
values were also significantly different, with chickens < ducks < turkeys < quail. Chickens had the higher CL
value in contrast to ducks. Differences by sex showed that duck females had a higher CL
value than the turkey and quail, whereas duck males had a CL
close to that of turkey. The ratio "AFBO production /AFB
-GSH production" follows the order duck>turkey>quail>chicken, in agreement with the known poultry sensitivity. The extremely high "AFB
epoxidation activity/ GST activity" ratio observed in ducks might be the explanation for the development of hepatocellular carcinoma in this species.</description><subject>Aflatoxin B1</subject><subject>Animals</subject><subject>Chickens</subject><subject>Ducks</subject><subject>Female</subject><subject>Glutathione Transferase</subject><subject>Liver</subject><subject>Male</subject><subject>Poultry</subject><subject>Quail</subject><subject>Turkeys</subject><issn>1525-3171</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2021</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkE1OwzAQhS0kREvhAGyQD0CKx85f2QHiT6oEErCuJsmkdUkcy3aBcExORCpgwYrVm9F78z1pGDsCMQUB6el6av16KoWE7S5VssPGkMgkUpDBiO17vxaDmabZHhupWEKcxemYfT64LlAZ9Ctxquth4l3Nl80mYFjpzhB_jIJD42ty6IeM-ehb4ri90KHnuERtfOBYNxi6d234BQc-iO02TXA995ZKTf6MOxoSA9GvtOUFhTci83_RizYUdMktOmwpkPMnHE010Lz2AU1JPHR_2w_Ybo2Np8MfnbDn66uny9tofn9zd3k-jyyIPEQqKZOsgEpADoAzkRcpikRKzFQlywIzBDkjKgHiRMmqqpGgyjMxOCmJVKkJO_7m2k3RUrWwTrfo-sXvc9UX5OmBHA</recordid><startdate>202108</startdate><enddate>202108</enddate><creator>Murcia, Hansen W</creator><creator>Diaz, Gonzalo J</creator><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope></search><sort><creationdate>202108</creationdate><title>Protective effect of glutathione S-transferase enzyme activity against aflatoxin B 1 in poultry species: relationship between glutathione S-transferase enzyme kinetic parameters, and resistance to aflatoxin B 1</title><author>Murcia, Hansen W ; Diaz, Gonzalo J</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-p108t-35c57b1d01811a908b6a0522a73d2cba7a129eec114532ddfae1d870ba76e0633</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2021</creationdate><topic>Aflatoxin B1</topic><topic>Animals</topic><topic>Chickens</topic><topic>Ducks</topic><topic>Female</topic><topic>Glutathione Transferase</topic><topic>Liver</topic><topic>Male</topic><topic>Poultry</topic><topic>Quail</topic><topic>Turkeys</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Murcia, Hansen W</creatorcontrib><creatorcontrib>Diaz, Gonzalo J</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><jtitle>Poultry science</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Murcia, Hansen W</au><au>Diaz, Gonzalo J</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Protective effect of glutathione S-transferase enzyme activity against aflatoxin B 1 in poultry species: relationship between glutathione S-transferase enzyme kinetic parameters, and resistance to aflatoxin B 1</atitle><jtitle>Poultry science</jtitle><addtitle>Poult Sci</addtitle><date>2021-08</date><risdate>2021</risdate><volume>100</volume><issue>8</issue><spage>101235</spage><pages>101235-</pages><eissn>1525-3171</eissn><abstract>Comparative studies designed to investigate the role of glutathione S-transferase (GST) activity on the enzyme catalyzed trapping of aflatoxin B
-8,9-epoxide (AFBO) with glutathione, and the relationship with aflatoxin B
(AFB
) resistance have not been conducted in poultry. Hepatic cytosolic fractions of chickens, quail, turkeys and ducks were used to measure in vitro the enzymatic parameters maximal velocity (V
), Michaelis-Menten constant (K
) and intrinsic clearance (CL
) for GST activity. AFB
used ranged from 2.0 to 157.5 µM and the AFB
-GSH produced was identified and quantitated by HPLC. Significant differences were found in GST V
values, being the highest in chickens, followed by quail, ducks and turkeys. The K
values were also significantly different, with chickens < ducks < turkeys < quail. Chickens had the higher CL
value in contrast to ducks. Differences by sex showed that duck females had a higher CL
value than the turkey and quail, whereas duck males had a CL
close to that of turkey. The ratio "AFBO production /AFB
-GSH production" follows the order duck>turkey>quail>chicken, in agreement with the known poultry sensitivity. The extremely high "AFB
epoxidation activity/ GST activity" ratio observed in ducks might be the explanation for the development of hepatocellular carcinoma in this species.</abstract><cop>England</cop><pmid>34214746</pmid><doi>10.1016/j.psj.2021.101235</doi><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | EISSN: 1525-3171 |
| ispartof | Poultry science, 2021-08, Vol.100 (8), p.101235 |
| issn | 1525-3171 |
| language | eng |
| recordid | cdi_pubmed_primary_34214746 |
| source | MEDLINE; PubMed Central(OpenAccess); Directory of Open Access Journals; Alma/SFX Local Collection; EZB Electronic Journals Library |
| subjects | Aflatoxin B1 Animals Chickens Ducks Female Glutathione Transferase Liver Male Poultry Quail Turkeys |
| title | Protective effect of glutathione S-transferase enzyme activity against aflatoxin B 1 in poultry species: relationship between glutathione S-transferase enzyme kinetic parameters, and resistance to aflatoxin B 1 |
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