Detection of novel sequence types and zoonotic transmission potentiality among strains of Shiga toxigenic Escherichia coli (STEC) from dairy calves, animal handlers and associated environments
Shiga toxigenic Escherichia coli (STEC) is one of the most important food-borne zoonotic bacterial pathogens responsible for causing gastrointestinal infections, haemorrhagic colitis and haemolytic uremic syndrome. The present study was aimed to isolate and characterize STEC from neonatal dairy ca...
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| Veröffentlicht in: | Brazilian journal of microbiology 2021-12, Vol.52 (4), p.2541-2546 |
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| container_title | Brazilian journal of microbiology |
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| creator | Angappan, Madesh Ghatak, Sandeep Milton, Arockiasamy Arun Prince Verma, Asha Kumari Inbaraj, Sophia Abhishek Chaudhuri, Pallab Agarwal, Rajesh Kumar Thomas, Prasad |
| description | Shiga toxigenic
Escherichia coli
(STEC) is one of the most important food-borne zoonotic bacterial pathogens responsible for causing gastrointestinal infections, haemorrhagic colitis and haemolytic uremic syndrome. The present study was aimed to isolate and characterize STEC from neonatal dairy calves, animal handlers and their surrounding environment and to establish the genetic relationship among isolates by multilocus sequence typing (MLST). A total number of 115 samples were collected and processed for the isolation of
E. coli
. The occurrence rate of
E. coli
was 92.2% (106/115), of which, 18 were typed as STEC. Antibacterial susceptibility analysis revealed 11 (61.1%) strains as multiple drug-resistant (MDR). MLST analysis has delineated 16 sequence types (STs) including nine novel STs. Among STs, ST58 dominated with three strains and was recovered from the environment and neonatal calves. Strains from neonatal calves and humans showed genetic relatedness with significant bootstrap support values indicative of zoonotic transmission potentiality. Analysis of 211 global isolates belonging to 61 STs indicated predominant STs (ST 21, ST 33 and ST 3416) that can be either host-specific (ST 33 and ST 3416) or can be shared among human and bovine hosts (ST 21). The MLST analysis indicates genetic relatedness among isolates and the results predispose inter-host transmission and zoonotic spread. |
| doi_str_mv | 10.1007/s42770-021-00561-9 |
| format | Article |
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Escherichia coli
(STEC) is one of the most important food-borne zoonotic bacterial pathogens responsible for causing gastrointestinal infections, haemorrhagic colitis and haemolytic uremic syndrome. The present study was aimed to isolate and characterize STEC from neonatal dairy calves, animal handlers and their surrounding environment and to establish the genetic relationship among isolates by multilocus sequence typing (MLST). A total number of 115 samples were collected and processed for the isolation of
E. coli
. The occurrence rate of
E. coli
was 92.2% (106/115), of which, 18 were typed as STEC. Antibacterial susceptibility analysis revealed 11 (61.1%) strains as multiple drug-resistant (MDR). MLST analysis has delineated 16 sequence types (STs) including nine novel STs. Among STs, ST58 dominated with three strains and was recovered from the environment and neonatal calves. Strains from neonatal calves and humans showed genetic relatedness with significant bootstrap support values indicative of zoonotic transmission potentiality. Analysis of 211 global isolates belonging to 61 STs indicated predominant STs (ST 21, ST 33 and ST 3416) that can be either host-specific (ST 33 and ST 3416) or can be shared among human and bovine hosts (ST 21). The MLST analysis indicates genetic relatedness among isolates and the results predispose inter-host transmission and zoonotic spread.</description><identifier>ISSN: 1517-8382</identifier><identifier>EISSN: 1678-4405</identifier><identifier>DOI: 10.1007/s42770-021-00561-9</identifier><identifier>PMID: 34241826</identifier><language>eng</language><publisher>Cham: Springer International Publishing</publisher><subject>Animals ; Anti-Bacterial Agents ; Antiinfectives and antibacterials ; Bacterial Zoonoses ; Biomedical and Life Sciences ; Calves ; Cattle - microbiology ; Colitis ; Disease transmission ; Drug resistance ; Drug Resistance, Multiple, Bacterial ; E coli ; Escherichia coli ; Escherichia coli Infections - veterinary ; Escherichia coli Proteins - genetics ; Food Microbiology ; Genetic analysis ; Genetic relationship ; Handlers ; Humans ; Life Sciences ; Medical Microbiology ; Microbial Ecology ; Microbial Genetics and Genomics ; Microbiology ; Multidrug resistance ; Multilocus Sequence Typing ; Mycology ; Neonates ; Shiga-Toxigenic Escherichia coli - classification ; Shiga-Toxigenic Escherichia coli - drug effects ; Strains (organisms) ; Veterinary Microbiology - Short Communication ; Zoonoses</subject><ispartof>Brazilian journal of microbiology, 2021-12, Vol.52 (4), p.2541-2546</ispartof><rights>Sociedade Brasileira de Microbiologia 2021</rights><rights>2021. Sociedade Brasileira de Microbiologia.</rights><rights>Sociedade Brasileira de Microbiologia 2021.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c474t-93dae8c99feb1dde4d7f180625cd0cbfceaba9120322dd5e998f7c4d0edbbd853</citedby><cites>FETCH-LOGICAL-c474t-93dae8c99feb1dde4d7f180625cd0cbfceaba9120322dd5e998f7c4d0edbbd853</cites><orcidid>0000-0001-5602-7710</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC8578500/pdf/$$EPDF$$P50$$Gpubmedcentral$$H</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC8578500/$$EHTML$$P50$$Gpubmedcentral$$H</linktohtml><link.rule.ids>230,314,727,780,784,885,27924,27925,41488,42557,51319,53791,53793</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/34241826$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Angappan, Madesh</creatorcontrib><creatorcontrib>Ghatak, Sandeep</creatorcontrib><creatorcontrib>Milton, Arockiasamy Arun Prince</creatorcontrib><creatorcontrib>Verma, Asha Kumari</creatorcontrib><creatorcontrib>Inbaraj, Sophia</creatorcontrib><creatorcontrib>Abhishek</creatorcontrib><creatorcontrib>Chaudhuri, Pallab</creatorcontrib><creatorcontrib>Agarwal, Rajesh Kumar</creatorcontrib><creatorcontrib>Thomas, Prasad</creatorcontrib><title>Detection of novel sequence types and zoonotic transmission potentiality among strains of Shiga toxigenic Escherichia coli (STEC) from dairy calves, animal handlers and associated environments</title><title>Brazilian journal of microbiology</title><addtitle>Braz J Microbiol</addtitle><addtitle>Braz J Microbiol</addtitle><description>Shiga toxigenic
Escherichia coli
(STEC) is one of the most important food-borne zoonotic bacterial pathogens responsible for causing gastrointestinal infections, haemorrhagic colitis and haemolytic uremic syndrome. The present study was aimed to isolate and characterize STEC from neonatal dairy calves, animal handlers and their surrounding environment and to establish the genetic relationship among isolates by multilocus sequence typing (MLST). A total number of 115 samples were collected and processed for the isolation of
E. coli
. The occurrence rate of
E. coli
was 92.2% (106/115), of which, 18 were typed as STEC. Antibacterial susceptibility analysis revealed 11 (61.1%) strains as multiple drug-resistant (MDR). MLST analysis has delineated 16 sequence types (STs) including nine novel STs. Among STs, ST58 dominated with three strains and was recovered from the environment and neonatal calves. Strains from neonatal calves and humans showed genetic relatedness with significant bootstrap support values indicative of zoonotic transmission potentiality. Analysis of 211 global isolates belonging to 61 STs indicated predominant STs (ST 21, ST 33 and ST 3416) that can be either host-specific (ST 33 and ST 3416) or can be shared among human and bovine hosts (ST 21). The MLST analysis indicates genetic relatedness among isolates and the results predispose inter-host transmission and zoonotic spread.</description><subject>Animals</subject><subject>Anti-Bacterial Agents</subject><subject>Antiinfectives and antibacterials</subject><subject>Bacterial Zoonoses</subject><subject>Biomedical and Life Sciences</subject><subject>Calves</subject><subject>Cattle - microbiology</subject><subject>Colitis</subject><subject>Disease transmission</subject><subject>Drug resistance</subject><subject>Drug Resistance, Multiple, Bacterial</subject><subject>E coli</subject><subject>Escherichia coli</subject><subject>Escherichia coli Infections - veterinary</subject><subject>Escherichia coli Proteins - genetics</subject><subject>Food Microbiology</subject><subject>Genetic analysis</subject><subject>Genetic relationship</subject><subject>Handlers</subject><subject>Humans</subject><subject>Life Sciences</subject><subject>Medical Microbiology</subject><subject>Microbial Ecology</subject><subject>Microbial Genetics and Genomics</subject><subject>Microbiology</subject><subject>Multidrug resistance</subject><subject>Multilocus Sequence Typing</subject><subject>Mycology</subject><subject>Neonates</subject><subject>Shiga-Toxigenic Escherichia coli - classification</subject><subject>Shiga-Toxigenic Escherichia coli - drug effects</subject><subject>Strains (organisms)</subject><subject>Veterinary Microbiology - Short Communication</subject><subject>Zoonoses</subject><issn>1517-8382</issn><issn>1678-4405</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2021</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kc9vFCEcxSdGY2v1H_BgSLxo4igwsAOXJma7_kiaeGg9Ewa-M0MzAyuwG9e_rn-arFOrXjxBwnvv88KrqucEvyUYt-8So22La0xJjTFfkVo-qE7JqhU1Y5g_LHdO2lo0gp5UT1K6wZhyzOjj6qRhlBFBV6fV7QVkMNkFj0KPfNjDhBJ824E3gPJhCwlpb9GPEHzIzqActU-zS-no2IYMPjs9uXxAeg5-QKkInE_HsKvRDRrl8N0N4It1k8wI0ZnRaWTC5NCrq-vN-jXqY5iR1S4ekNHTHtKbgnSzntBY0BPEpYJOKRinM1gEfu9i8HOBp6fVo15PCZ7dnWfV1w-b6_Wn-vLLx8_r95e1YS3LtWysBmGk7KEj1gKzbU8EXlFuLDZdb0B3WhKKG0qt5SCl6FvDLAbbdVbw5qw6X3K3u24Gawo76kltY2kaDypop_598W5UQ9grwVvBMS4BL-8CYij_m7K6CbvoS2dFueRclh2PGLqoTAwpRejvCQSr4-pqWV0Vtfq1upLF9OLvbveW3zMXQbMIUnnyA8Q_7P_E_gTpqb_Z</recordid><startdate>20211201</startdate><enddate>20211201</enddate><creator>Angappan, Madesh</creator><creator>Ghatak, Sandeep</creator><creator>Milton, Arockiasamy Arun Prince</creator><creator>Verma, Asha Kumari</creator><creator>Inbaraj, Sophia</creator><creator>Abhishek</creator><creator>Chaudhuri, Pallab</creator><creator>Agarwal, Rajesh Kumar</creator><creator>Thomas, Prasad</creator><general>Springer International Publishing</general><general>Springer Nature B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>7U9</scope><scope>C1K</scope><scope>H94</scope><scope>K9.</scope><scope>M7N</scope><scope>5PM</scope><orcidid>https://orcid.org/0000-0001-5602-7710</orcidid></search><sort><creationdate>20211201</creationdate><title>Detection of novel sequence types and zoonotic transmission potentiality among strains of Shiga toxigenic Escherichia coli (STEC) from dairy calves, animal handlers and associated environments</title><author>Angappan, Madesh ; Ghatak, Sandeep ; Milton, Arockiasamy Arun Prince ; Verma, Asha Kumari ; Inbaraj, Sophia ; Abhishek ; Chaudhuri, Pallab ; Agarwal, Rajesh Kumar ; Thomas, Prasad</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c474t-93dae8c99feb1dde4d7f180625cd0cbfceaba9120322dd5e998f7c4d0edbbd853</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2021</creationdate><topic>Animals</topic><topic>Anti-Bacterial Agents</topic><topic>Antiinfectives and antibacterials</topic><topic>Bacterial Zoonoses</topic><topic>Biomedical and Life Sciences</topic><topic>Calves</topic><topic>Cattle - microbiology</topic><topic>Colitis</topic><topic>Disease transmission</topic><topic>Drug resistance</topic><topic>Drug Resistance, Multiple, Bacterial</topic><topic>E coli</topic><topic>Escherichia coli</topic><topic>Escherichia coli Infections - veterinary</topic><topic>Escherichia coli Proteins - genetics</topic><topic>Food Microbiology</topic><topic>Genetic analysis</topic><topic>Genetic relationship</topic><topic>Handlers</topic><topic>Humans</topic><topic>Life Sciences</topic><topic>Medical Microbiology</topic><topic>Microbial Ecology</topic><topic>Microbial Genetics and Genomics</topic><topic>Microbiology</topic><topic>Multidrug resistance</topic><topic>Multilocus Sequence Typing</topic><topic>Mycology</topic><topic>Neonates</topic><topic>Shiga-Toxigenic Escherichia coli - classification</topic><topic>Shiga-Toxigenic Escherichia coli - drug effects</topic><topic>Strains (organisms)</topic><topic>Veterinary Microbiology - Short Communication</topic><topic>Zoonoses</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Angappan, Madesh</creatorcontrib><creatorcontrib>Ghatak, Sandeep</creatorcontrib><creatorcontrib>Milton, Arockiasamy Arun Prince</creatorcontrib><creatorcontrib>Verma, Asha Kumari</creatorcontrib><creatorcontrib>Inbaraj, Sophia</creatorcontrib><creatorcontrib>Abhishek</creatorcontrib><creatorcontrib>Chaudhuri, Pallab</creatorcontrib><creatorcontrib>Agarwal, Rajesh Kumar</creatorcontrib><creatorcontrib>Thomas, Prasad</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Virology and AIDS Abstracts</collection><collection>Environmental Sciences and Pollution Management</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Brazilian journal of microbiology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Angappan, Madesh</au><au>Ghatak, Sandeep</au><au>Milton, Arockiasamy Arun Prince</au><au>Verma, Asha Kumari</au><au>Inbaraj, Sophia</au><au>Abhishek</au><au>Chaudhuri, Pallab</au><au>Agarwal, Rajesh Kumar</au><au>Thomas, Prasad</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Detection of novel sequence types and zoonotic transmission potentiality among strains of Shiga toxigenic Escherichia coli (STEC) from dairy calves, animal handlers and associated environments</atitle><jtitle>Brazilian journal of microbiology</jtitle><stitle>Braz J Microbiol</stitle><addtitle>Braz J Microbiol</addtitle><date>2021-12-01</date><risdate>2021</risdate><volume>52</volume><issue>4</issue><spage>2541</spage><epage>2546</epage><pages>2541-2546</pages><issn>1517-8382</issn><eissn>1678-4405</eissn><abstract>Shiga toxigenic
Escherichia coli
(STEC) is one of the most important food-borne zoonotic bacterial pathogens responsible for causing gastrointestinal infections, haemorrhagic colitis and haemolytic uremic syndrome. The present study was aimed to isolate and characterize STEC from neonatal dairy calves, animal handlers and their surrounding environment and to establish the genetic relationship among isolates by multilocus sequence typing (MLST). A total number of 115 samples were collected and processed for the isolation of
E. coli
. The occurrence rate of
E. coli
was 92.2% (106/115), of which, 18 were typed as STEC. Antibacterial susceptibility analysis revealed 11 (61.1%) strains as multiple drug-resistant (MDR). MLST analysis has delineated 16 sequence types (STs) including nine novel STs. Among STs, ST58 dominated with three strains and was recovered from the environment and neonatal calves. Strains from neonatal calves and humans showed genetic relatedness with significant bootstrap support values indicative of zoonotic transmission potentiality. Analysis of 211 global isolates belonging to 61 STs indicated predominant STs (ST 21, ST 33 and ST 3416) that can be either host-specific (ST 33 and ST 3416) or can be shared among human and bovine hosts (ST 21). The MLST analysis indicates genetic relatedness among isolates and the results predispose inter-host transmission and zoonotic spread.</abstract><cop>Cham</cop><pub>Springer International Publishing</pub><pmid>34241826</pmid><doi>10.1007/s42770-021-00561-9</doi><tpages>6</tpages><orcidid>https://orcid.org/0000-0001-5602-7710</orcidid><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1517-8382 |
| ispartof | Brazilian journal of microbiology, 2021-12, Vol.52 (4), p.2541-2546 |
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| subjects | Animals Anti-Bacterial Agents Antiinfectives and antibacterials Bacterial Zoonoses Biomedical and Life Sciences Calves Cattle - microbiology Colitis Disease transmission Drug resistance Drug Resistance, Multiple, Bacterial E coli Escherichia coli Escherichia coli Infections - veterinary Escherichia coli Proteins - genetics Food Microbiology Genetic analysis Genetic relationship Handlers Humans Life Sciences Medical Microbiology Microbial Ecology Microbial Genetics and Genomics Microbiology Multidrug resistance Multilocus Sequence Typing Mycology Neonates Shiga-Toxigenic Escherichia coli - classification Shiga-Toxigenic Escherichia coli - drug effects Strains (organisms) Veterinary Microbiology - Short Communication Zoonoses |
| title | Detection of novel sequence types and zoonotic transmission potentiality among strains of Shiga toxigenic Escherichia coli (STEC) from dairy calves, animal handlers and associated environments |
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