Characterization of Growth and Cell Cycle Events Affected by Light Intensity in the Green Alga Parachlorella kessleri: A New Model for Cell Cycle Research

Multiple fission is a cell cycle variation leading to the production of more than two daughter cells. Here, we used synchronized cultures of the chlorococcal green alga Parachlorella kessleri to study its growth and pattern of cell division under varying light intensities. The time courses of DNA re...

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Veröffentlicht in:Biomolecules (Basel, Switzerland) Switzerland), 2021-06, Vol.11 (6), p.891
Hauptverfasser: Zachleder, Vilém, Ivanov, Ivan N., Kselíková, Veronika, Bialevich, Vitali, Vítová, Milada, Ota, Shuhei, Takeshita, Tsuyoshi, Kawano, Shigeyuki, Bišová, Kateřina
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container_title Biomolecules (Basel, Switzerland)
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creator Zachleder, Vilém
Ivanov, Ivan N.
Kselíková, Veronika
Bialevich, Vitali
Vítová, Milada
Ota, Shuhei
Takeshita, Tsuyoshi
Kawano, Shigeyuki
Bišová, Kateřina
description Multiple fission is a cell cycle variation leading to the production of more than two daughter cells. Here, we used synchronized cultures of the chlorococcal green alga Parachlorella kessleri to study its growth and pattern of cell division under varying light intensities. The time courses of DNA replication, nuclear and cellular division, cell size, total RNA, protein content, dry matter and accumulation of starch were observed at incident light intensities of 110, 250 and 500 µmol photons m−2s−1. Furthermore, we studied the effect of deuterated water on Parachlorella kessleri growth and division, to mimic the effect of stress. We describe a novel multiple fission cell cycle pattern characterized by multiple rounds of DNA replication leading to cell polyploidization. Once completed, multiple nuclear divisions were performed with each of them, immediately followed by protoplast fission, terminated by the formation of daughter cells. The multiple fission cell cycle was represented by several consecutive doublings of growth parameters, each leading to the start of a reproductive sequence. The number of growth doublings increased with increasing light intensity and led to division into more daughter cells. This study establishes the baseline for cell cycle research at the molecular level as well as for potential biotechnological applications, particularly directed synthesis of (deuterated) starch and/or neutral lipids as carbon and energy reserves.
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subjects Algae
Biotechnology
Carbon dioxide
Cell cycle
cell cycle pattern
Cell division
Cell size
Deoxyribonucleic acid
DNA
DNA biosynthesis
Dry matter
Energy
energy reserves
growth processes
Light
Light intensity
Lipids
Metabolism
Parachlorella kessleri
Photons
Polyploidy
Replication
reproduction events
Starch
Sulfur
title Characterization of Growth and Cell Cycle Events Affected by Light Intensity in the Green Alga Parachlorella kessleri: A New Model for Cell Cycle Research
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