Suppression of NtZIP4A / B Changes Zn and Cd Root-to-Shoot Translocation in a Zn/Cd Status-Dependent Manner
In tobacco, the efficiency of Zn translocation to shoots depends on Zn/Cd status. Previous studies pointed to the specific contribution of root parts in the regulation of this process, as well as the role of / (from the ZIP family; Zrt Irt-like Proteins). Here, to verify this hypothesis, / RNAi line...
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| Veröffentlicht in: | International journal of molecular sciences 2021-05, Vol.22 (10), p.5355 |
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| creator | Maślińska-Gromadka, Karolina Barabasz, Anna Palusińska, Małgorzata Kozak, Katarzyna Antosiewicz, Danuta Maria |
| description | In tobacco, the efficiency of Zn translocation to shoots depends on Zn/Cd status. Previous studies pointed to the specific contribution of root parts in the regulation of this process, as well as the role of
/
(from the ZIP family; Zrt Irt-like Proteins). Here, to verify this hypothesis,
/
RNAi lines were generated. Then, in plants exposed to combinations of Zn and Cd concentrations in the medium, the consequences of
/
suppression for the translocation of both metals were determined. Furthermore, the apical, middle, and basal root parts were examined for accumulation of both metals, for Zn localization (using Zinpyr-1), and for modifications of the expression pattern of
genes. Our results confirmed the role of
/
in the control of Zn/Cd-status-dependent transfer of both metals to shoots. Furthermore, they indicated that the middle and basal root parts contributed to the regulation of this process by acting as a reservoir for excess Zn and Cd. Expression studies identified several candidate
genes that interact with
/
in the root in regulating Zn and Cd translocation to the shoot, primarily
in the basal root part and
in the middle one. |
| doi_str_mv | 10.3390/ijms22105355 |
| format | Article |
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/
(from the ZIP family; Zrt Irt-like Proteins). Here, to verify this hypothesis,
/
RNAi lines were generated. Then, in plants exposed to combinations of Zn and Cd concentrations in the medium, the consequences of
/
suppression for the translocation of both metals were determined. Furthermore, the apical, middle, and basal root parts were examined for accumulation of both metals, for Zn localization (using Zinpyr-1), and for modifications of the expression pattern of
genes. Our results confirmed the role of
/
in the control of Zn/Cd-status-dependent transfer of both metals to shoots. Furthermore, they indicated that the middle and basal root parts contributed to the regulation of this process by acting as a reservoir for excess Zn and Cd. Expression studies identified several candidate
genes that interact with
/
in the root in regulating Zn and Cd translocation to the shoot, primarily
in the basal root part and
in the middle one.</description><identifier>ISSN: 1422-0067</identifier><identifier>ISSN: 1661-6596</identifier><identifier>EISSN: 1422-0067</identifier><identifier>DOI: 10.3390/ijms22105355</identifier><identifier>PMID: 34069632</identifier><language>eng</language><publisher>Switzerland: MDPI AG</publisher><subject>Adenosine Triphosphatases - metabolism ; Biological Transport - genetics ; Cadmium ; Cadmium - metabolism ; Cation Transport Proteins - genetics ; Cation Transport Proteins - metabolism ; Efficiency ; Gene expression ; Gene Expression Regulation, Plant - genetics ; Genes ; GUS ; Homeostasis ; Localization ; Metals ; Nicotiana - genetics ; Nicotiana - metabolism ; NtZIP4 ; Plant Proteins - genetics ; Plant Roots - genetics ; Plant Roots - metabolism ; Plant Shoots - metabolism ; Plants, Genetically Modified - metabolism ; RNA-mediated interference ; root-to-shoot translocation ; Tobacco ; zinc ; Zinc - metabolism</subject><ispartof>International journal of molecular sciences, 2021-05, Vol.22 (10), p.5355</ispartof><rights>2021 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>2021 by the authors. 2021</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c478t-7685ec0145aac1418fe85587dbc2f6c33f939787950ab3b8e146a8664a2ce74b3</citedby><cites>FETCH-LOGICAL-c478t-7685ec0145aac1418fe85587dbc2f6c33f939787950ab3b8e146a8664a2ce74b3</cites><orcidid>0000-0003-1803-1836 ; 0000-0003-4921-0378 ; 0000-0002-0399-5280</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC8161331/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC8161331/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,727,780,784,885,27924,27925,53791,53793</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/34069632$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Maślińska-Gromadka, Karolina</creatorcontrib><creatorcontrib>Barabasz, Anna</creatorcontrib><creatorcontrib>Palusińska, Małgorzata</creatorcontrib><creatorcontrib>Kozak, Katarzyna</creatorcontrib><creatorcontrib>Antosiewicz, Danuta Maria</creatorcontrib><title>Suppression of NtZIP4A / B Changes Zn and Cd Root-to-Shoot Translocation in a Zn/Cd Status-Dependent Manner</title><title>International journal of molecular sciences</title><addtitle>Int J Mol Sci</addtitle><description>In tobacco, the efficiency of Zn translocation to shoots depends on Zn/Cd status. Previous studies pointed to the specific contribution of root parts in the regulation of this process, as well as the role of
/
(from the ZIP family; Zrt Irt-like Proteins). Here, to verify this hypothesis,
/
RNAi lines were generated. Then, in plants exposed to combinations of Zn and Cd concentrations in the medium, the consequences of
/
suppression for the translocation of both metals were determined. Furthermore, the apical, middle, and basal root parts were examined for accumulation of both metals, for Zn localization (using Zinpyr-1), and for modifications of the expression pattern of
genes. Our results confirmed the role of
/
in the control of Zn/Cd-status-dependent transfer of both metals to shoots. Furthermore, they indicated that the middle and basal root parts contributed to the regulation of this process by acting as a reservoir for excess Zn and Cd. Expression studies identified several candidate
genes that interact with
/
in the root in regulating Zn and Cd translocation to the shoot, primarily
in the basal root part and
in the middle one.</description><subject>Adenosine Triphosphatases - metabolism</subject><subject>Biological Transport - genetics</subject><subject>Cadmium</subject><subject>Cadmium - metabolism</subject><subject>Cation Transport Proteins - genetics</subject><subject>Cation Transport Proteins - metabolism</subject><subject>Efficiency</subject><subject>Gene expression</subject><subject>Gene Expression Regulation, Plant - genetics</subject><subject>Genes</subject><subject>GUS</subject><subject>Homeostasis</subject><subject>Localization</subject><subject>Metals</subject><subject>Nicotiana - genetics</subject><subject>Nicotiana - metabolism</subject><subject>NtZIP4</subject><subject>Plant Proteins - genetics</subject><subject>Plant Roots - genetics</subject><subject>Plant Roots - metabolism</subject><subject>Plant Shoots - metabolism</subject><subject>Plants, Genetically Modified - metabolism</subject><subject>RNA-mediated interference</subject><subject>root-to-shoot translocation</subject><subject>Tobacco</subject><subject>zinc</subject><subject>Zinc - metabolism</subject><issn>1422-0067</issn><issn>1661-6596</issn><issn>1422-0067</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2021</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>8G5</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><sourceid>GUQSH</sourceid><sourceid>M2O</sourceid><sourceid>DOA</sourceid><recordid>eNpdkstvEzEQh1cIRB9w44wsceHQJX7be0Eq4RWpPETKpRdr1utNNmzsYHuR-O9xSKlSTh55Pn-asX5V9YzgV4w1eDZstolSggUT4kF1SjilNcZSPTyqT6qzlDYYU0ZF87g6YRzLRjJ6Wv1YTrtddCkNwaPQo8_5ZvGVX6IZeoPma_Arl9CNR-A7NO_QtxBynUO9XJcCXUfwaQwW8v7xUKiCzgq2zJCnVL91O-c75zP6BN67-KR61MOY3NPb87z6_v7d9fxjffXlw2J-eVVbrnSuldTCWUy4ALCEE907LYRWXWtpLy1jfcMapVUjMLSs1Y5wCVpKDtQ6xVt2Xi0O3i7AxuzisIX42wQYzN-LEFcGYh7s6IzWHRDdUtuzngNm0LcCetBcNVZia4vr9cG1m9qt62zZJsJ4T3q_44e1WYVfRhNJGCNF8PJWEMPPyaVstkOybhzBuzAlQwWTXCqqaUFf_IduwhR9-ao9RYXSnKlCXRwoG0NK0fV3wxBs9okwx4ko-PPjBe7gfxFgfwCBQrBx</recordid><startdate>20210519</startdate><enddate>20210519</enddate><creator>Maślińska-Gromadka, Karolina</creator><creator>Barabasz, Anna</creator><creator>Palusińska, Małgorzata</creator><creator>Kozak, Katarzyna</creator><creator>Antosiewicz, Danuta Maria</creator><general>MDPI AG</general><general>MDPI</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>8G5</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BENPR</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>GUQSH</scope><scope>K9.</scope><scope>M0S</scope><scope>M1P</scope><scope>M2O</scope><scope>MBDVC</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>Q9U</scope><scope>7X8</scope><scope>5PM</scope><scope>DOA</scope><orcidid>https://orcid.org/0000-0003-1803-1836</orcidid><orcidid>https://orcid.org/0000-0003-4921-0378</orcidid><orcidid>https://orcid.org/0000-0002-0399-5280</orcidid></search><sort><creationdate>20210519</creationdate><title>Suppression of NtZIP4A / B Changes Zn and Cd Root-to-Shoot Translocation in a Zn/Cd Status-Dependent Manner</title><author>Maślińska-Gromadka, Karolina ; Barabasz, Anna ; Palusińska, Małgorzata ; Kozak, Katarzyna ; Antosiewicz, Danuta Maria</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c478t-7685ec0145aac1418fe85587dbc2f6c33f939787950ab3b8e146a8664a2ce74b3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2021</creationdate><topic>Adenosine Triphosphatases - metabolism</topic><topic>Biological Transport - genetics</topic><topic>Cadmium</topic><topic>Cadmium - metabolism</topic><topic>Cation Transport Proteins - genetics</topic><topic>Cation Transport Proteins - metabolism</topic><topic>Efficiency</topic><topic>Gene expression</topic><topic>Gene Expression Regulation, Plant - genetics</topic><topic>Genes</topic><topic>GUS</topic><topic>Homeostasis</topic><topic>Localization</topic><topic>Metals</topic><topic>Nicotiana - genetics</topic><topic>Nicotiana - metabolism</topic><topic>NtZIP4</topic><topic>Plant Proteins - genetics</topic><topic>Plant Roots - genetics</topic><topic>Plant Roots - metabolism</topic><topic>Plant Shoots - metabolism</topic><topic>Plants, Genetically Modified - metabolism</topic><topic>RNA-mediated interference</topic><topic>root-to-shoot translocation</topic><topic>Tobacco</topic><topic>zinc</topic><topic>Zinc - metabolism</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Maślińska-Gromadka, Karolina</creatorcontrib><creatorcontrib>Barabasz, Anna</creatorcontrib><creatorcontrib>Palusińska, Małgorzata</creatorcontrib><creatorcontrib>Kozak, Katarzyna</creatorcontrib><creatorcontrib>Antosiewicz, Danuta Maria</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>Research Library (Alumni Edition)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>ProQuest Central</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>Research Library Prep</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Research Library</collection><collection>Research Library (Corporate)</collection><collection>Publicly Available Content Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>ProQuest Central Basic</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><collection>DOAJ Directory of Open Access Journals</collection><jtitle>International journal of molecular sciences</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Maślińska-Gromadka, Karolina</au><au>Barabasz, Anna</au><au>Palusińska, Małgorzata</au><au>Kozak, Katarzyna</au><au>Antosiewicz, Danuta Maria</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Suppression of NtZIP4A / B Changes Zn and Cd Root-to-Shoot Translocation in a Zn/Cd Status-Dependent Manner</atitle><jtitle>International journal of molecular sciences</jtitle><addtitle>Int J Mol Sci</addtitle><date>2021-05-19</date><risdate>2021</risdate><volume>22</volume><issue>10</issue><spage>5355</spage><pages>5355-</pages><issn>1422-0067</issn><issn>1661-6596</issn><eissn>1422-0067</eissn><abstract>In tobacco, the efficiency of Zn translocation to shoots depends on Zn/Cd status. Previous studies pointed to the specific contribution of root parts in the regulation of this process, as well as the role of
/
(from the ZIP family; Zrt Irt-like Proteins). Here, to verify this hypothesis,
/
RNAi lines were generated. Then, in plants exposed to combinations of Zn and Cd concentrations in the medium, the consequences of
/
suppression for the translocation of both metals were determined. Furthermore, the apical, middle, and basal root parts were examined for accumulation of both metals, for Zn localization (using Zinpyr-1), and for modifications of the expression pattern of
genes. Our results confirmed the role of
/
in the control of Zn/Cd-status-dependent transfer of both metals to shoots. Furthermore, they indicated that the middle and basal root parts contributed to the regulation of this process by acting as a reservoir for excess Zn and Cd. Expression studies identified several candidate
genes that interact with
/
in the root in regulating Zn and Cd translocation to the shoot, primarily
in the basal root part and
in the middle one.</abstract><cop>Switzerland</cop><pub>MDPI AG</pub><pmid>34069632</pmid><doi>10.3390/ijms22105355</doi><orcidid>https://orcid.org/0000-0003-1803-1836</orcidid><orcidid>https://orcid.org/0000-0003-4921-0378</orcidid><orcidid>https://orcid.org/0000-0002-0399-5280</orcidid><oa>free_for_read</oa></addata></record> |
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| source | MEDLINE; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; MDPI - Multidisciplinary Digital Publishing Institute; PubMed Central |
| subjects | Adenosine Triphosphatases - metabolism Biological Transport - genetics Cadmium Cadmium - metabolism Cation Transport Proteins - genetics Cation Transport Proteins - metabolism Efficiency Gene expression Gene Expression Regulation, Plant - genetics Genes GUS Homeostasis Localization Metals Nicotiana - genetics Nicotiana - metabolism NtZIP4 Plant Proteins - genetics Plant Roots - genetics Plant Roots - metabolism Plant Shoots - metabolism Plants, Genetically Modified - metabolism RNA-mediated interference root-to-shoot translocation Tobacco zinc Zinc - metabolism |
| title | Suppression of NtZIP4A / B Changes Zn and Cd Root-to-Shoot Translocation in a Zn/Cd Status-Dependent Manner |
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