Investigation of culturable human gut mycobiota from the segamat community in Johor, Malaysia
Although several studies have already been carried out in investigating the general profile of the gut mycobiome across several countries, there has yet to be an officially established baseline of a healthy human gut mycobiome, to the best of our knowledge. Microbial composition within the gastroint...
Gespeichert in:
| Veröffentlicht in: | World journal of microbiology & biotechnology 2021-07, Vol.37 (7), p.113-113, Article 113 |
|---|---|
| Hauptverfasser: | , , , , , , , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 113 |
|---|---|
| container_issue | 7 |
| container_start_page | 113 |
| container_title | World journal of microbiology & biotechnology |
| container_volume | 37 |
| creator | Huët, Marie Andrea Laetitia Wong, Li Wen Goh, Calvin Bok Sun Hussain, Md Hamed Muzahid, Nazmul Hasan Dwiyanto, Jacky Lee, Shaun Wen Huey Ayub, Qasim Reidpath, Daniel Lee, Sui Mae Rahman, Sadequr Tan, Joash Ban Lee |
| description | Although several studies have already been carried out in investigating the general profile of the gut mycobiome across several countries, there has yet to be an officially established baseline of a healthy human gut mycobiome, to the best of our knowledge. Microbial composition within the gastrointestinal tract differ across individuals worldwide, and most human gut fungi studies concentrate specifically on individuals from developed countries or diseased cohorts. The present study is the first culture-dependent community study assessing the prevalence and diversity of gut fungi among different ethnic groups from South East Asia. Samples were obtained from a multi-ethnic semi-rural community from Segamat in southern Malaysia. Faecal samples were screened for culturable fungi and questionnaire data analysis was performed. Culturable fungi were present in 45% of the participants’ stool samples. Ethnicity had an impact on fungal prevalence and density in stool samples. The prevalence of resistance to fluconazole, itraconazole, voriconazole and 5-fluorocytosine, from the Segamat community, were 14%, 14%, 11% and 7% respectively. It was found that Jakun individuals had lower levels of antifungal resistance irrespective of the drug tested, and male participants had more fluconazole resistant yeast in their stool samples. Two novel point mutations were identified in the
ERG11
gene from one azole resistant
Candida glabrata
, suggesting a possible cause of the occurrence of antifungal resistant isolates in the participant’s faecal sample.
Graphic abstract |
| doi_str_mv | 10.1007/s11274-021-03083-6 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_webof</sourceid><recordid>TN_cdi_webofscience_primary_000659185800001</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>2539211780</sourcerecordid><originalsourceid>FETCH-LOGICAL-c389t-48d71a58a919776134790d3fbdae2f0bd505d3dabd84725b615139372eebf093</originalsourceid><addsrcrecordid>eNqNkU1v1DAQhi1ERZeFP8DJEhckSBnbcWwf0YqPolZcekWWkzi7rhK7-KNo_z3eBoHEAfXkOTyPZ-YdhF4RuCAA4n0ihIq2AUoaYCBZ0z1BG8IFa0AJ-hRtQHHVMKXYOXqe0i1A1RR7hs5ZS2rN-AZ9v_T3NmW3N9kFj8OEhzLnEk0_W3woi_F4XzJejkPoXcgGTzEsOB8sTnZvFpPxEJaleJeP2Hn8NRxCfIevzWyOyZkX6Gwyc7Ivf79bdPPp483uS3P17fPl7sNVMzCpctPKURDDpVFECdER1goFI5v60Vg6QT9y4CMbTT_KVlDed4QTppig1vZT3WiL3qzf3sXwo9R19OLSYOfZeBtK0pQzRQkREir6-h_0NpTo63AnSkoJtPbfIrpSQwwpRTvpu-gWE4-agD5lr9fsdc1eP2Svuyq9XaWftg9TGpz1g_0jAkDHFZFc1gpOLeTj6Z3LD_fZheJzVdmqpor7vY1_d_jPeL8AzXSldQ</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2538880261</pqid></control><display><type>article</type><title>Investigation of culturable human gut mycobiota from the segamat community in Johor, Malaysia</title><source>Web of Science - Science Citation Index Expanded - 2021<img src="https://exlibris-pub.s3.amazonaws.com/fromwos-v2.jpg" /></source><source>SpringerLink Journals - AutoHoldings</source><creator>Huët, Marie Andrea Laetitia ; Wong, Li Wen ; Goh, Calvin Bok Sun ; Hussain, Md Hamed ; Muzahid, Nazmul Hasan ; Dwiyanto, Jacky ; Lee, Shaun Wen Huey ; Ayub, Qasim ; Reidpath, Daniel ; Lee, Sui Mae ; Rahman, Sadequr ; Tan, Joash Ban Lee</creator><creatorcontrib>Huët, Marie Andrea Laetitia ; Wong, Li Wen ; Goh, Calvin Bok Sun ; Hussain, Md Hamed ; Muzahid, Nazmul Hasan ; Dwiyanto, Jacky ; Lee, Shaun Wen Huey ; Ayub, Qasim ; Reidpath, Daniel ; Lee, Sui Mae ; Rahman, Sadequr ; Tan, Joash Ban Lee</creatorcontrib><description>Although several studies have already been carried out in investigating the general profile of the gut mycobiome across several countries, there has yet to be an officially established baseline of a healthy human gut mycobiome, to the best of our knowledge. Microbial composition within the gastrointestinal tract differ across individuals worldwide, and most human gut fungi studies concentrate specifically on individuals from developed countries or diseased cohorts. The present study is the first culture-dependent community study assessing the prevalence and diversity of gut fungi among different ethnic groups from South East Asia. Samples were obtained from a multi-ethnic semi-rural community from Segamat in southern Malaysia. Faecal samples were screened for culturable fungi and questionnaire data analysis was performed. Culturable fungi were present in 45% of the participants’ stool samples. Ethnicity had an impact on fungal prevalence and density in stool samples. The prevalence of resistance to fluconazole, itraconazole, voriconazole and 5-fluorocytosine, from the Segamat community, were 14%, 14%, 11% and 7% respectively. It was found that Jakun individuals had lower levels of antifungal resistance irrespective of the drug tested, and male participants had more fluconazole resistant yeast in their stool samples. Two novel point mutations were identified in the
ERG11
gene from one azole resistant
Candida glabrata
, suggesting a possible cause of the occurrence of antifungal resistant isolates in the participant’s faecal sample.
Graphic abstract</description><identifier>ISSN: 0959-3993</identifier><identifier>EISSN: 1573-0972</identifier><identifier>DOI: 10.1007/s11274-021-03083-6</identifier><identifier>PMID: 34101035</identifier><language>eng</language><publisher>Dordrecht: Springer Netherlands</publisher><subject>Applied Microbiology ; Biochemistry ; Biomedical and Life Sciences ; Biotechnology ; Biotechnology & Applied Microbiology ; Data analysis ; Developed countries ; Digestive system ; Environmental Engineering/Biotechnology ; ERG11 gene ; Ethnic factors ; Feces ; Fluconazole ; Fungi ; Fungicides ; Gastrointestinal system ; Gastrointestinal tract ; Itraconazole ; Life Sciences ; Life Sciences & Biomedicine ; Microbiology ; Microorganisms ; Minority & ethnic groups ; Mutation ; Original Paper ; Rural areas ; Rural communities ; Science & Technology ; Voriconazole ; Yeasts</subject><ispartof>World journal of microbiology & biotechnology, 2021-07, Vol.37 (7), p.113-113, Article 113</ispartof><rights>The Author(s), under exclusive licence to Springer Nature B.V. 2021</rights><rights>The Author(s), under exclusive licence to Springer Nature B.V. 2021.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>true</woscitedreferencessubscribed><woscitedreferencescount>7</woscitedreferencescount><woscitedreferencesoriginalsourcerecordid>wos000659185800001</woscitedreferencesoriginalsourcerecordid><citedby>FETCH-LOGICAL-c389t-48d71a58a919776134790d3fbdae2f0bd505d3dabd84725b615139372eebf093</citedby><cites>FETCH-LOGICAL-c389t-48d71a58a919776134790d3fbdae2f0bd505d3dabd84725b615139372eebf093</cites><orcidid>0000-0001-6623-2427 ; 0000-0001-8066-0487 ; 0000-0001-7361-6576 ; 0000-0003-2343-4373 ; 0000-0002-1224-5553</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s11274-021-03083-6$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s11274-021-03083-6$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>315,782,786,27931,27932,39265,41495,42564,51326</link.rule.ids></links><search><creatorcontrib>Huët, Marie Andrea Laetitia</creatorcontrib><creatorcontrib>Wong, Li Wen</creatorcontrib><creatorcontrib>Goh, Calvin Bok Sun</creatorcontrib><creatorcontrib>Hussain, Md Hamed</creatorcontrib><creatorcontrib>Muzahid, Nazmul Hasan</creatorcontrib><creatorcontrib>Dwiyanto, Jacky</creatorcontrib><creatorcontrib>Lee, Shaun Wen Huey</creatorcontrib><creatorcontrib>Ayub, Qasim</creatorcontrib><creatorcontrib>Reidpath, Daniel</creatorcontrib><creatorcontrib>Lee, Sui Mae</creatorcontrib><creatorcontrib>Rahman, Sadequr</creatorcontrib><creatorcontrib>Tan, Joash Ban Lee</creatorcontrib><title>Investigation of culturable human gut mycobiota from the segamat community in Johor, Malaysia</title><title>World journal of microbiology & biotechnology</title><addtitle>World J Microbiol Biotechnol</addtitle><addtitle>WORLD J MICROB BIOT</addtitle><description>Although several studies have already been carried out in investigating the general profile of the gut mycobiome across several countries, there has yet to be an officially established baseline of a healthy human gut mycobiome, to the best of our knowledge. Microbial composition within the gastrointestinal tract differ across individuals worldwide, and most human gut fungi studies concentrate specifically on individuals from developed countries or diseased cohorts. The present study is the first culture-dependent community study assessing the prevalence and diversity of gut fungi among different ethnic groups from South East Asia. Samples were obtained from a multi-ethnic semi-rural community from Segamat in southern Malaysia. Faecal samples were screened for culturable fungi and questionnaire data analysis was performed. Culturable fungi were present in 45% of the participants’ stool samples. Ethnicity had an impact on fungal prevalence and density in stool samples. The prevalence of resistance to fluconazole, itraconazole, voriconazole and 5-fluorocytosine, from the Segamat community, were 14%, 14%, 11% and 7% respectively. It was found that Jakun individuals had lower levels of antifungal resistance irrespective of the drug tested, and male participants had more fluconazole resistant yeast in their stool samples. Two novel point mutations were identified in the
ERG11
gene from one azole resistant
Candida glabrata
, suggesting a possible cause of the occurrence of antifungal resistant isolates in the participant’s faecal sample.
Graphic abstract</description><subject>Applied Microbiology</subject><subject>Biochemistry</subject><subject>Biomedical and Life Sciences</subject><subject>Biotechnology</subject><subject>Biotechnology & Applied Microbiology</subject><subject>Data analysis</subject><subject>Developed countries</subject><subject>Digestive system</subject><subject>Environmental Engineering/Biotechnology</subject><subject>ERG11 gene</subject><subject>Ethnic factors</subject><subject>Feces</subject><subject>Fluconazole</subject><subject>Fungi</subject><subject>Fungicides</subject><subject>Gastrointestinal system</subject><subject>Gastrointestinal tract</subject><subject>Itraconazole</subject><subject>Life Sciences</subject><subject>Life Sciences & Biomedicine</subject><subject>Microbiology</subject><subject>Microorganisms</subject><subject>Minority & ethnic groups</subject><subject>Mutation</subject><subject>Original Paper</subject><subject>Rural areas</subject><subject>Rural communities</subject><subject>Science & Technology</subject><subject>Voriconazole</subject><subject>Yeasts</subject><issn>0959-3993</issn><issn>1573-0972</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2021</creationdate><recordtype>article</recordtype><sourceid>HGBXW</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNqNkU1v1DAQhi1ERZeFP8DJEhckSBnbcWwf0YqPolZcekWWkzi7rhK7-KNo_z3eBoHEAfXkOTyPZ-YdhF4RuCAA4n0ihIq2AUoaYCBZ0z1BG8IFa0AJ-hRtQHHVMKXYOXqe0i1A1RR7hs5ZS2rN-AZ9v_T3NmW3N9kFj8OEhzLnEk0_W3woi_F4XzJejkPoXcgGTzEsOB8sTnZvFpPxEJaleJeP2Hn8NRxCfIevzWyOyZkX6Gwyc7Ivf79bdPPp483uS3P17fPl7sNVMzCpctPKURDDpVFECdER1goFI5v60Vg6QT9y4CMbTT_KVlDed4QTppig1vZT3WiL3qzf3sXwo9R19OLSYOfZeBtK0pQzRQkREir6-h_0NpTo63AnSkoJtPbfIrpSQwwpRTvpu-gWE4-agD5lr9fsdc1eP2Svuyq9XaWftg9TGpz1g_0jAkDHFZFc1gpOLeTj6Z3LD_fZheJzVdmqpor7vY1_d_jPeL8AzXSldQ</recordid><startdate>20210701</startdate><enddate>20210701</enddate><creator>Huët, Marie Andrea Laetitia</creator><creator>Wong, Li Wen</creator><creator>Goh, Calvin Bok Sun</creator><creator>Hussain, Md Hamed</creator><creator>Muzahid, Nazmul Hasan</creator><creator>Dwiyanto, Jacky</creator><creator>Lee, Shaun Wen Huey</creator><creator>Ayub, Qasim</creator><creator>Reidpath, Daniel</creator><creator>Lee, Sui Mae</creator><creator>Rahman, Sadequr</creator><creator>Tan, Joash Ban Lee</creator><general>Springer Netherlands</general><general>Springer Nature</general><general>Springer Nature B.V</general><scope>BLEPL</scope><scope>DTL</scope><scope>HGBXW</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7QL</scope><scope>7T7</scope><scope>7TB</scope><scope>7TK</scope><scope>7U5</scope><scope>7U9</scope><scope>7WY</scope><scope>7WZ</scope><scope>7X7</scope><scope>7XB</scope><scope>87Z</scope><scope>88A</scope><scope>88E</scope><scope>88I</scope><scope>8AO</scope><scope>8FD</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>8FL</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BEZIV</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FRNLG</scope><scope>FYUFA</scope><scope>F~G</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K60</scope><scope>K6~</scope><scope>K9.</scope><scope>L.-</scope><scope>L7M</scope><scope>LK8</scope><scope>M0C</scope><scope>M0S</scope><scope>M1P</scope><scope>M2P</scope><scope>M7N</scope><scope>M7P</scope><scope>P64</scope><scope>PQBIZ</scope><scope>PQBZA</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>Q9U</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0001-6623-2427</orcidid><orcidid>https://orcid.org/0000-0001-8066-0487</orcidid><orcidid>https://orcid.org/0000-0001-7361-6576</orcidid><orcidid>https://orcid.org/0000-0003-2343-4373</orcidid><orcidid>https://orcid.org/0000-0002-1224-5553</orcidid></search><sort><creationdate>20210701</creationdate><title>Investigation of culturable human gut mycobiota from the segamat community in Johor, Malaysia</title><author>Huët, Marie Andrea Laetitia ; Wong, Li Wen ; Goh, Calvin Bok Sun ; Hussain, Md Hamed ; Muzahid, Nazmul Hasan ; Dwiyanto, Jacky ; Lee, Shaun Wen Huey ; Ayub, Qasim ; Reidpath, Daniel ; Lee, Sui Mae ; Rahman, Sadequr ; Tan, Joash Ban Lee</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c389t-48d71a58a919776134790d3fbdae2f0bd505d3dabd84725b615139372eebf093</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2021</creationdate><topic>Applied Microbiology</topic><topic>Biochemistry</topic><topic>Biomedical and Life Sciences</topic><topic>Biotechnology</topic><topic>Biotechnology & Applied Microbiology</topic><topic>Data analysis</topic><topic>Developed countries</topic><topic>Digestive system</topic><topic>Environmental Engineering/Biotechnology</topic><topic>ERG11 gene</topic><topic>Ethnic factors</topic><topic>Feces</topic><topic>Fluconazole</topic><topic>Fungi</topic><topic>Fungicides</topic><topic>Gastrointestinal system</topic><topic>Gastrointestinal tract</topic><topic>Itraconazole</topic><topic>Life Sciences</topic><topic>Life Sciences & Biomedicine</topic><topic>Microbiology</topic><topic>Microorganisms</topic><topic>Minority & ethnic groups</topic><topic>Mutation</topic><topic>Original Paper</topic><topic>Rural areas</topic><topic>Rural communities</topic><topic>Science & Technology</topic><topic>Voriconazole</topic><topic>Yeasts</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Huët, Marie Andrea Laetitia</creatorcontrib><creatorcontrib>Wong, Li Wen</creatorcontrib><creatorcontrib>Goh, Calvin Bok Sun</creatorcontrib><creatorcontrib>Hussain, Md Hamed</creatorcontrib><creatorcontrib>Muzahid, Nazmul Hasan</creatorcontrib><creatorcontrib>Dwiyanto, Jacky</creatorcontrib><creatorcontrib>Lee, Shaun Wen Huey</creatorcontrib><creatorcontrib>Ayub, Qasim</creatorcontrib><creatorcontrib>Reidpath, Daniel</creatorcontrib><creatorcontrib>Lee, Sui Mae</creatorcontrib><creatorcontrib>Rahman, Sadequr</creatorcontrib><creatorcontrib>Tan, Joash Ban Lee</creatorcontrib><collection>Web of Science Core Collection</collection><collection>Science Citation Index Expanded</collection><collection>Web of Science - Science Citation Index Expanded - 2021</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Mechanical & Transportation Engineering Abstracts</collection><collection>Neurosciences Abstracts</collection><collection>Solid State and Superconductivity Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Access via ABI/INFORM (ProQuest)</collection><collection>ABI/INFORM Global (PDF only)</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>ABI/INFORM Global (Alumni Edition)</collection><collection>Biology Database (Alumni Edition)</collection><collection>Medical Database (Alumni Edition)</collection><collection>Science Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ABI/INFORM Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Business Premium Collection</collection><collection>Natural Science Collection</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Engineering Research Database</collection><collection>Business Premium Collection (Alumni)</collection><collection>Health Research Premium Collection</collection><collection>ABI/INFORM Global (Corporate)</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Business Collection (Alumni Edition)</collection><collection>ProQuest Business Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>ABI/INFORM Professional Advanced</collection><collection>Advanced Technologies Database with Aerospace</collection><collection>ProQuest Biological Science Collection</collection><collection>ABI/INFORM Global</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Science Database</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biological Science Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>ProQuest One Business</collection><collection>ProQuest One Business (Alumni)</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>ProQuest Central Basic</collection><collection>MEDLINE - Academic</collection><jtitle>World journal of microbiology & biotechnology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Huët, Marie Andrea Laetitia</au><au>Wong, Li Wen</au><au>Goh, Calvin Bok Sun</au><au>Hussain, Md Hamed</au><au>Muzahid, Nazmul Hasan</au><au>Dwiyanto, Jacky</au><au>Lee, Shaun Wen Huey</au><au>Ayub, Qasim</au><au>Reidpath, Daniel</au><au>Lee, Sui Mae</au><au>Rahman, Sadequr</au><au>Tan, Joash Ban Lee</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Investigation of culturable human gut mycobiota from the segamat community in Johor, Malaysia</atitle><jtitle>World journal of microbiology & biotechnology</jtitle><stitle>World J Microbiol Biotechnol</stitle><stitle>WORLD J MICROB BIOT</stitle><date>2021-07-01</date><risdate>2021</risdate><volume>37</volume><issue>7</issue><spage>113</spage><epage>113</epage><pages>113-113</pages><artnum>113</artnum><issn>0959-3993</issn><eissn>1573-0972</eissn><abstract>Although several studies have already been carried out in investigating the general profile of the gut mycobiome across several countries, there has yet to be an officially established baseline of a healthy human gut mycobiome, to the best of our knowledge. Microbial composition within the gastrointestinal tract differ across individuals worldwide, and most human gut fungi studies concentrate specifically on individuals from developed countries or diseased cohorts. The present study is the first culture-dependent community study assessing the prevalence and diversity of gut fungi among different ethnic groups from South East Asia. Samples were obtained from a multi-ethnic semi-rural community from Segamat in southern Malaysia. Faecal samples were screened for culturable fungi and questionnaire data analysis was performed. Culturable fungi were present in 45% of the participants’ stool samples. Ethnicity had an impact on fungal prevalence and density in stool samples. The prevalence of resistance to fluconazole, itraconazole, voriconazole and 5-fluorocytosine, from the Segamat community, were 14%, 14%, 11% and 7% respectively. It was found that Jakun individuals had lower levels of antifungal resistance irrespective of the drug tested, and male participants had more fluconazole resistant yeast in their stool samples. Two novel point mutations were identified in the
ERG11
gene from one azole resistant
Candida glabrata
, suggesting a possible cause of the occurrence of antifungal resistant isolates in the participant’s faecal sample.
Graphic abstract</abstract><cop>Dordrecht</cop><pub>Springer Netherlands</pub><pmid>34101035</pmid><doi>10.1007/s11274-021-03083-6</doi><tpages>15</tpages><orcidid>https://orcid.org/0000-0001-6623-2427</orcidid><orcidid>https://orcid.org/0000-0001-8066-0487</orcidid><orcidid>https://orcid.org/0000-0001-7361-6576</orcidid><orcidid>https://orcid.org/0000-0003-2343-4373</orcidid><orcidid>https://orcid.org/0000-0002-1224-5553</orcidid></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0959-3993 |
| ispartof | World journal of microbiology & biotechnology, 2021-07, Vol.37 (7), p.113-113, Article 113 |
| issn | 0959-3993 1573-0972 |
| language | eng |
| recordid | cdi_webofscience_primary_000659185800001 |
| source | Web of Science - Science Citation Index Expanded - 2021<img src="https://exlibris-pub.s3.amazonaws.com/fromwos-v2.jpg" />; SpringerLink Journals - AutoHoldings |
| subjects | Applied Microbiology Biochemistry Biomedical and Life Sciences Biotechnology Biotechnology & Applied Microbiology Data analysis Developed countries Digestive system Environmental Engineering/Biotechnology ERG11 gene Ethnic factors Feces Fluconazole Fungi Fungicides Gastrointestinal system Gastrointestinal tract Itraconazole Life Sciences Life Sciences & Biomedicine Microbiology Microorganisms Minority & ethnic groups Mutation Original Paper Rural areas Rural communities Science & Technology Voriconazole Yeasts |
| title | Investigation of culturable human gut mycobiota from the segamat community in Johor, Malaysia |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-09T06%3A54%3A21IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_webof&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Investigation%20of%20culturable%20human%20gut%20mycobiota%20from%20the%20segamat%20community%20in%20Johor,%20Malaysia&rft.jtitle=World%20journal%20of%20microbiology%20&%20biotechnology&rft.au=Hu%C3%ABt,%20Marie%20Andrea%20Laetitia&rft.date=2021-07-01&rft.volume=37&rft.issue=7&rft.spage=113&rft.epage=113&rft.pages=113-113&rft.artnum=113&rft.issn=0959-3993&rft.eissn=1573-0972&rft_id=info:doi/10.1007/s11274-021-03083-6&rft_dat=%3Cproquest_webof%3E2539211780%3C/proquest_webof%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=2538880261&rft_id=info:pmid/34101035&rfr_iscdi=true |