Proposal of an iELISA for Mycoplasma bovis diagnosis in dairy cattle and associated risk factors
Mycoplasma bovis is a highly contagious agent associated with several pathologies in cattle. The detection of reactive antibodies to M. bovis by Indirect Enzyme-Linked Immunosorbent Assay (iELISA) identifies if there was an exposure to the microorganism. The current study aimed to optimize an iELISA...
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| Veröffentlicht in: | Arquivo brasileiro de medicina veterinária e zootecnia 2021-03, Vol.73 (2), p.293-301 |
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| creator | Pires, D. R. Morais, A. C. N. Cunha, N. C. Machado, L. S. Barbosa, L. F. C. Mendonca, J. F. M. Balaro, M. F. A. Santos, J. P. C. Souza, G. N. Barreto, M. L. Nascimento, E. R. |
| description | Mycoplasma bovis is a highly contagious agent associated with several pathologies in cattle. The detection of reactive antibodies to M. bovis by Indirect Enzyme-Linked Immunosorbent Assay (iELISA) identifies if there was an exposure to the microorganism. The current study aimed to optimize an iELISA from M. bovis total cell antigen, applying it to bovine serum samples, and to evaluate risk factors. Serum samples were obtained from 400 cows from 17 herds from Southeast Brazil. In the optimization of iELISA, the following was established: 2 mu g/mL of antigen, sera dilution 1:300, and conjugate dilution 1:15000. The frequency was 62.3% (249/400) of reactive animals and 100% (17/17) of reactive herds. Risk factors were: herds with more than 100 animals (OR= 3.1; CI= 95%); Holstein breed (OR= 72.5; CI= 95%); cows (OR= 29.7; CI= 95%); intensive breeding system (OR= 3.3; CI= 95%); associated small ruminant production (OR= 4.4; CI= 95%); milk production above 500L (OR= 2.9; CI= 95%); no quarantine (OR= 1.5; CI= 95%); mechanical milking (OR= 5.5; CI= 95%) and cases of mastitis (OR= 5.5; CI= 95%). The proposed iELISA was able to detect antibodies reactive to M. bovis in bovine serum. Knowledge of these risk factors can assist in the implementation of prophylactic measures. |
| doi_str_mv | 10.1590/1678-4162-12140 |
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R. ; Morais, A. C. N. ; Cunha, N. C. ; Machado, L. S. ; Barbosa, L. F. C. ; Mendonca, J. F. M. ; Balaro, M. F. A. ; Santos, J. P. C. ; Souza, G. N. ; Barreto, M. L. ; Nascimento, E. R.</creator><creatorcontrib>Pires, D. R. ; Morais, A. C. N. ; Cunha, N. C. ; Machado, L. S. ; Barbosa, L. F. C. ; Mendonca, J. F. M. ; Balaro, M. F. A. ; Santos, J. P. C. ; Souza, G. N. ; Barreto, M. L. ; Nascimento, E. R.</creatorcontrib><description>Mycoplasma bovis is a highly contagious agent associated with several pathologies in cattle. The detection of reactive antibodies to M. bovis by Indirect Enzyme-Linked Immunosorbent Assay (iELISA) identifies if there was an exposure to the microorganism. The current study aimed to optimize an iELISA from M. bovis total cell antigen, applying it to bovine serum samples, and to evaluate risk factors. Serum samples were obtained from 400 cows from 17 herds from Southeast Brazil. In the optimization of iELISA, the following was established: 2 mu g/mL of antigen, sera dilution 1:300, and conjugate dilution 1:15000. The frequency was 62.3% (249/400) of reactive animals and 100% (17/17) of reactive herds. Risk factors were: herds with more than 100 animals (OR= 3.1; CI= 95%); Holstein breed (OR= 72.5; CI= 95%); cows (OR= 29.7; CI= 95%); intensive breeding system (OR= 3.3; CI= 95%); associated small ruminant production (OR= 4.4; CI= 95%); milk production above 500L (OR= 2.9; CI= 95%); no quarantine (OR= 1.5; CI= 95%); mechanical milking (OR= 5.5; CI= 95%) and cases of mastitis (OR= 5.5; CI= 95%). The proposed iELISA was able to detect antibodies reactive to M. bovis in bovine serum. Knowledge of these risk factors can assist in the implementation of prophylactic measures.</description><identifier>ISSN: 0102-0935</identifier><identifier>ISSN: 1678-4162</identifier><identifier>EISSN: 1678-4162</identifier><identifier>DOI: 10.1590/1678-4162-12140</identifier><language>eng</language><publisher>MINAS GERAIS: Arquivo Brasileiro Medicina Veterinaria Zootecnia</publisher><subject>cows ; Holstein breed ; Life Sciences & Biomedicine ; mastitis ; mycoplasmosis ; Science & Technology ; serology ; VETERINARY SCIENCES</subject><ispartof>Arquivo brasileiro de medicina veterinária e zootecnia, 2021-03, Vol.73 (2), p.293-301</ispartof><rights>This work is licensed under a Creative Commons Attribution 4.0 International License.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>true</woscitedreferencessubscribed><woscitedreferencescount>4</woscitedreferencescount><woscitedreferencesoriginalsourcerecordid>wos000658235200004</woscitedreferencesoriginalsourcerecordid><citedby>FETCH-LOGICAL-c387t-e48cee46fbb72964cc3668dfb807ccf10b8574842d19592580d8feeb117297a3</citedby><cites>FETCH-LOGICAL-c387t-e48cee46fbb72964cc3668dfb807ccf10b8574842d19592580d8feeb117297a3</cites><orcidid>0000-0002-6748-7218 ; 0000-0002-2122-8819 ; 0000-0002-1725-1995 ; 0000-0003-0582-5098 ; 0000-0003-4117-195X ; 0000-0002-8198-7964 ; 0000-0002-1413-4582 ; 0000-0003-2316-8933 ; 0000-0001-6971-7200 ; 0000-0001-9780-9911 ; 0000-0003-0049-6133</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>230,315,782,786,866,887,2104,2116,27931,27932,39265</link.rule.ids></links><search><creatorcontrib>Pires, D. R.</creatorcontrib><creatorcontrib>Morais, A. C. N.</creatorcontrib><creatorcontrib>Cunha, N. C.</creatorcontrib><creatorcontrib>Machado, L. S.</creatorcontrib><creatorcontrib>Barbosa, L. F. C.</creatorcontrib><creatorcontrib>Mendonca, J. F. M.</creatorcontrib><creatorcontrib>Balaro, M. F. A.</creatorcontrib><creatorcontrib>Santos, J. P. C.</creatorcontrib><creatorcontrib>Souza, G. N.</creatorcontrib><creatorcontrib>Barreto, M. L.</creatorcontrib><creatorcontrib>Nascimento, E. R.</creatorcontrib><title>Proposal of an iELISA for Mycoplasma bovis diagnosis in dairy cattle and associated risk factors</title><title>Arquivo brasileiro de medicina veterinária e zootecnia</title><addtitle>ARQ BRAS MED VET ZOO</addtitle><addtitle>Arq. Bras. Med. Vet. Zootec</addtitle><description>Mycoplasma bovis is a highly contagious agent associated with several pathologies in cattle. The detection of reactive antibodies to M. bovis by Indirect Enzyme-Linked Immunosorbent Assay (iELISA) identifies if there was an exposure to the microorganism. The current study aimed to optimize an iELISA from M. bovis total cell antigen, applying it to bovine serum samples, and to evaluate risk factors. Serum samples were obtained from 400 cows from 17 herds from Southeast Brazil. In the optimization of iELISA, the following was established: 2 mu g/mL of antigen, sera dilution 1:300, and conjugate dilution 1:15000. The frequency was 62.3% (249/400) of reactive animals and 100% (17/17) of reactive herds. Risk factors were: herds with more than 100 animals (OR= 3.1; CI= 95%); Holstein breed (OR= 72.5; CI= 95%); cows (OR= 29.7; CI= 95%); intensive breeding system (OR= 3.3; CI= 95%); associated small ruminant production (OR= 4.4; CI= 95%); milk production above 500L (OR= 2.9; CI= 95%); no quarantine (OR= 1.5; CI= 95%); mechanical milking (OR= 5.5; CI= 95%) and cases of mastitis (OR= 5.5; CI= 95%). The proposed iELISA was able to detect antibodies reactive to M. bovis in bovine serum. Knowledge of these risk factors can assist in the implementation of prophylactic measures.</description><subject>cows</subject><subject>Holstein breed</subject><subject>Life Sciences & Biomedicine</subject><subject>mastitis</subject><subject>mycoplasmosis</subject><subject>Science & Technology</subject><subject>serology</subject><subject>VETERINARY SCIENCES</subject><issn>0102-0935</issn><issn>1678-4162</issn><issn>1678-4162</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2021</creationdate><recordtype>article</recordtype><sourceid>HGBXW</sourceid><sourceid>DOA</sourceid><recordid>eNqNkUGPFCEQhYnRxHH17JW76V2ggYbjZrLqJGM02b0jVMOGsbdrAr2a-ffSO2bOEhIqFb5XlfcI-cjZNVeW3XA9mE5yLTouuGSvyObSeU02jDPRMdurt-RdrQfGhLSWb8jPHwWPWP1EMVE_03y3393f0oSFfjsBHidfnzwN-DtXOmb_OGNtVZ7p6HM5UfDLMsUGjtTXipD9Ekdacv1Fk4cFS31P3iQ_1fjh33tFHj7fPWy_dvvvX3bb230HvRmWLkoDMUqdQhiE1RKg19qMKRg2ACTOglGDNFKM3CorlGGjSTEGztv3wfdXZHeWHdEf3LHkJ19ODn12Lw0sj86XJcMUndWqGQNJKQjSmKbWB4gaRNOOAXTTuj5rVchxQnfA5zK33d396qJbXRRMcNZMbNf2Dbg5A1Cw1hLTZQHO3BqOW6NwaxTuJZxGmDPxJwZM65wZ4oVqyloZsY5pR27z4peM8xaf56Whn_4f7f8CN5efcQ</recordid><startdate>20210301</startdate><enddate>20210301</enddate><creator>Pires, D. 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The current study aimed to optimize an iELISA from M. bovis total cell antigen, applying it to bovine serum samples, and to evaluate risk factors. Serum samples were obtained from 400 cows from 17 herds from Southeast Brazil. In the optimization of iELISA, the following was established: 2 mu g/mL of antigen, sera dilution 1:300, and conjugate dilution 1:15000. The frequency was 62.3% (249/400) of reactive animals and 100% (17/17) of reactive herds. Risk factors were: herds with more than 100 animals (OR= 3.1; CI= 95%); Holstein breed (OR= 72.5; CI= 95%); cows (OR= 29.7; CI= 95%); intensive breeding system (OR= 3.3; CI= 95%); associated small ruminant production (OR= 4.4; CI= 95%); milk production above 500L (OR= 2.9; CI= 95%); no quarantine (OR= 1.5; CI= 95%); mechanical milking (OR= 5.5; CI= 95%) and cases of mastitis (OR= 5.5; CI= 95%). The proposed iELISA was able to detect antibodies reactive to M. bovis in bovine serum. Knowledge of these risk factors can assist in the implementation of prophylactic measures.</abstract><cop>MINAS GERAIS</cop><pub>Arquivo Brasileiro Medicina Veterinaria Zootecnia</pub><doi>10.1590/1678-4162-12140</doi><tpages>9</tpages><orcidid>https://orcid.org/0000-0002-6748-7218</orcidid><orcidid>https://orcid.org/0000-0002-2122-8819</orcidid><orcidid>https://orcid.org/0000-0002-1725-1995</orcidid><orcidid>https://orcid.org/0000-0003-0582-5098</orcidid><orcidid>https://orcid.org/0000-0003-4117-195X</orcidid><orcidid>https://orcid.org/0000-0002-8198-7964</orcidid><orcidid>https://orcid.org/0000-0002-1413-4582</orcidid><orcidid>https://orcid.org/0000-0003-2316-8933</orcidid><orcidid>https://orcid.org/0000-0001-6971-7200</orcidid><orcidid>https://orcid.org/0000-0001-9780-9911</orcidid><orcidid>https://orcid.org/0000-0003-0049-6133</orcidid><oa>free_for_read</oa></addata></record> |
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| subjects | cows Holstein breed Life Sciences & Biomedicine mastitis mycoplasmosis Science & Technology serology VETERINARY SCIENCES |
| title | Proposal of an iELISA for Mycoplasma bovis diagnosis in dairy cattle and associated risk factors |
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