Glycoengineering Chinese hamster ovary cells: a short history
Biotherapeutic glycoproteins have revolutionised the field of pharmaceuticals, with new discoveries and continuous improvements underpinning the rapid growth of this industry. N-glycosylation is a critical quality attribute of biotherapeutic glycoproteins that influences the efficacy, half-life and...
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| Veröffentlicht in: | Biochemical Society transactions 2021-04, Vol.49 (2), p.915-931 |
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| container_title | Biochemical Society transactions |
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| creator | Donini, Roberto Haslam, Stuart M Kontoravdi, Cleo |
| description | Biotherapeutic glycoproteins have revolutionised the field of pharmaceuticals, with new discoveries and continuous improvements underpinning the rapid growth of this industry. N-glycosylation is a critical quality attribute of biotherapeutic glycoproteins that influences the efficacy, half-life and immunogenicity of these drugs. This review will focus on the advances and future directions of remodelling N-glycosylation in Chinese hamster ovary (CHO) cells, which are the workhorse of recombinant biotherapeutic production, with particular emphasis on antibody products, using strategies such as cell line and protein backbone engineering. |
| doi_str_mv | 10.1042/BST20200840 |
| format | Article |
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| identifier | ISSN: 0300-5127 |
| ispartof | Biochemical Society transactions, 2021-04, Vol.49 (2), p.915-931 |
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| language | eng |
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| source | MEDLINE; Portland Press Electronic Journals |
| subjects | Animals Biotechnology CHO Cells Cricetinae Cricetulus Glycobiology Glycomics - methods Glycoproteins - genetics Glycoproteins - metabolism Glycosylation Metabolic Engineering - methods Polysaccharides - chemistry Polysaccharides - metabolism Post-Translational Modifications Protein Engineering - methods Proteomics - methods Recombinant Proteins - metabolism Review |
| title | Glycoengineering Chinese hamster ovary cells: a short history |
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