Facial soft tissue depth measurements in cone-beam computed tomography: A study of a Brazilian sample

•Detailed description for soft tissue depth measurements in Cone-beam computed tomography.•Test in a sample of 100 anonymous subjects with new tissue depth charts.•Use of databank files with no exposure of subjects to radiation.•A Soft tissue measures from standing living individuals reducing tissue alterations and displacement [1–49].•Age, sex and facial pattern analysis.•Comparison to previous studies in the literature. Forensic facial reconstruction (approximation) (FFR) is an aid to human identification when no presumed identity is available. It is based on average soft tissue thickness (STT) applied onto a skull. These averages vary at each landmark according to sex, ancestry, and Facial Growth Patterns (FGP). To obtain the reference, there are different protocols and conditions, such as needles in cadavers, ultrasound, magnetic resonance, and computer tomography. Using cone-beam computed tomography (CBCT), landmark placement and direction of measurement are critical. The purpose of this article is to detailly describe a method for STT measurement in CBCT DICOM files and to test it in the analysis of a multi-ancestral Brazilian sample, subdivided into sex, age and FGP. A sample of 100 (50 male and 50 female) CBCT exams were selected and the volume was primarily been repositioned to cephalometric standards. A detailed description of the location and measurement direction of 32 landmarks was provided and granted good reproducibility of the measurements. The averages were assessed by sex, age, and FGP. There were significant differences between males and females. The CBCT measuring protocol provided standardization of measurements and is a method to be used in future researches. Advantages of CBCT, such as the subject’s upright position and adjustable volume positioning, also justify its usage. Limited differences of STT were related to age and FGP. The updated Brazilian soft tissue thickness chart is a reliable source of data for forensic and clinical purposes.