Lateral Hypothalamic Area Glutamatergic Neurons and Their Projections to the Lateral Habenula Modulate the Anesthetic Potency of Isoflurane in Mice
The lateral hypothalamic area (LHA) plays a pivotal role in regulating consciousness transition, in which orexinergic neurons, GABAergic neurons, and melanin-concentrating hormone neurons are involved. Glutamatergic neurons have a large population in the LHA, but their anesthesia-related effect has...
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| Veröffentlicht in: | Neuroscience bulletin 2021-07, Vol.37 (7), p.934-946 |
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| creator | Zhao, Shiyi Li, Rui Li, Huiming Wang, Sa Zhang, Xinxin Wang, Dan Guo, Juan Li, Huihui Li, Ao Tong, Tingting Zhong, Haixing Yang, Qianzi Dong, Hailong |
| description | The lateral hypothalamic area (LHA) plays a pivotal role in regulating consciousness transition, in which orexinergic neurons, GABAergic neurons, and melanin-concentrating hormone neurons are involved. Glutamatergic neurons have a large population in the LHA, but their anesthesia-related effect has not been explored. Here, we found that genetic ablation of LHA glutamatergic neurons shortened the induction time and prolonged the recovery time of isoflurane anesthesia in mice. In contrast, chemogenetic activation of LHA glutamatergic neurons increased the time to anesthesia and decreased the time to recovery. Optogenetic activation of LHA glutamatergic neurons during the maintenance of anesthesia reduced the burst suppression pattern of the electroencephalogram (EEG) and shifted EEG features to an arousal pattern. Photostimulation of LHA glutamatergic projections to the lateral habenula (LHb) also facilitated the emergence from anesthesia and the transition of anesthesia depth to a lighter level. Collectively, LHA glutamatergic neurons and their projections to the LHb regulate anesthetic potency and EEG features. |
| doi_str_mv | 10.1007/s12264-021-00674-z |
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Glutamatergic neurons have a large population in the LHA, but their anesthesia-related effect has not been explored. Here, we found that genetic ablation of LHA glutamatergic neurons shortened the induction time and prolonged the recovery time of isoflurane anesthesia in mice. In contrast, chemogenetic activation of LHA glutamatergic neurons increased the time to anesthesia and decreased the time to recovery. Optogenetic activation of LHA glutamatergic neurons during the maintenance of anesthesia reduced the burst suppression pattern of the electroencephalogram (EEG) and shifted EEG features to an arousal pattern. Photostimulation of LHA glutamatergic projections to the lateral habenula (LHb) also facilitated the emergence from anesthesia and the transition of anesthesia depth to a lighter level. Collectively, LHA glutamatergic neurons and their projections to the LHb regulate anesthetic potency and EEG features.</description><identifier>ISSN: 1673-7067</identifier><identifier>EISSN: 1995-8218</identifier><identifier>DOI: 10.1007/s12264-021-00674-z</identifier><identifier>PMID: 33847915</identifier><language>eng</language><publisher>Singapore: Springer Singapore</publisher><subject>Anatomy ; Anesthesia ; Anesthesiology ; Biomedical and Life Sciences ; Biomedicine ; Brain ; Electroencephalography ; GABA ; Human Physiology ; Isoflurane ; Life Sciences & Biomedicine ; Luteinizing hormone ; Neurology ; Neurons ; Neurosciences ; Neurosciences & Neurology ; Original ; Original Article ; Pain Medicine ; Science & Technology</subject><ispartof>Neuroscience bulletin, 2021-07, Vol.37 (7), p.934-946</ispartof><rights>The Author(s) 2021</rights><rights>COPYRIGHT 2021 Springer</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>true</woscitedreferencessubscribed><woscitedreferencescount>21</woscitedreferencescount><woscitedreferencesoriginalsourcerecordid>wos000639713500002</woscitedreferencesoriginalsourcerecordid><citedby>FETCH-LOGICAL-c513t-19a609051f1de5616e6f1cc697021481bbd85859d55ef78bf85d50e75fafef03</citedby><cites>FETCH-LOGICAL-c513t-19a609051f1de5616e6f1cc697021481bbd85859d55ef78bf85d50e75fafef03</cites><orcidid>0000-0001-8984-9067</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC8275739/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC8275739/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,315,728,781,785,886,27929,27930,39263,41493,42562,51324,53796,53798</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/33847915$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Zhao, Shiyi</creatorcontrib><creatorcontrib>Li, Rui</creatorcontrib><creatorcontrib>Li, Huiming</creatorcontrib><creatorcontrib>Wang, Sa</creatorcontrib><creatorcontrib>Zhang, Xinxin</creatorcontrib><creatorcontrib>Wang, Dan</creatorcontrib><creatorcontrib>Guo, Juan</creatorcontrib><creatorcontrib>Li, Huihui</creatorcontrib><creatorcontrib>Li, Ao</creatorcontrib><creatorcontrib>Tong, Tingting</creatorcontrib><creatorcontrib>Zhong, Haixing</creatorcontrib><creatorcontrib>Yang, Qianzi</creatorcontrib><creatorcontrib>Dong, Hailong</creatorcontrib><title>Lateral Hypothalamic Area Glutamatergic Neurons and Their Projections to the Lateral Habenula Modulate the Anesthetic Potency of Isoflurane in Mice</title><title>Neuroscience bulletin</title><addtitle>Neurosci. Bull</addtitle><addtitle>NEUROSCI BULL</addtitle><addtitle>Neurosci Bull</addtitle><description>The lateral hypothalamic area (LHA) plays a pivotal role in regulating consciousness transition, in which orexinergic neurons, GABAergic neurons, and melanin-concentrating hormone neurons are involved. Glutamatergic neurons have a large population in the LHA, but their anesthesia-related effect has not been explored. Here, we found that genetic ablation of LHA glutamatergic neurons shortened the induction time and prolonged the recovery time of isoflurane anesthesia in mice. In contrast, chemogenetic activation of LHA glutamatergic neurons increased the time to anesthesia and decreased the time to recovery. Optogenetic activation of LHA glutamatergic neurons during the maintenance of anesthesia reduced the burst suppression pattern of the electroencephalogram (EEG) and shifted EEG features to an arousal pattern. Photostimulation of LHA glutamatergic projections to the lateral habenula (LHb) also facilitated the emergence from anesthesia and the transition of anesthesia depth to a lighter level. Collectively, LHA glutamatergic neurons and their projections to the LHb regulate anesthetic potency and EEG features.</description><subject>Anatomy</subject><subject>Anesthesia</subject><subject>Anesthesiology</subject><subject>Biomedical and Life Sciences</subject><subject>Biomedicine</subject><subject>Brain</subject><subject>Electroencephalography</subject><subject>GABA</subject><subject>Human Physiology</subject><subject>Isoflurane</subject><subject>Life Sciences & Biomedicine</subject><subject>Luteinizing hormone</subject><subject>Neurology</subject><subject>Neurons</subject><subject>Neurosciences</subject><subject>Neurosciences & Neurology</subject><subject>Original</subject><subject>Original Article</subject><subject>Pain Medicine</subject><subject>Science & Technology</subject><issn>1673-7067</issn><issn>1995-8218</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2021</creationdate><recordtype>article</recordtype><sourceid>C6C</sourceid><sourceid>HGBXW</sourceid><recordid>eNqNUsFu1DAQjRCIlsIPcECWuCChFDuO4-SCtFpBW2kLPezdcpLxrldZe7EdUPsb_DCTpqyohBDyYayZ955nPC_LXjN6ziiVHyIriqrMacFySitZ5ndPslPWNCKvC1Y_xXsleS6xdJK9iHGHICp5-Tw74bwuZcPEafZzpRMEPZDL24NPWz3ove3IIoAmF8OY9H4qbzD1BcbgXSTa9WS9BRvITfA76JKdssmTtAVyFNMtuHHQ5Nr3GBLcVxcOIsaEajc-getuiTfkKnozjEE7INaRa9vBy-yZ0UOEVw_xLFt__rReXuarrxdXy8Uq7wTjKWeNrmhDBTOsB1GxCirDuq5qJP5IWbO27WtRi6YXAoysW1OLXlCQwmgDhvKz7OMsexjbPfQduIS9q0Owex1ulddWPa44u1Ub_13VhRSSNyjw7kEg-G8jzqb2NnYwDDiLH6MqBCt4yRs2vfV2hm70AMo641Gxm-BqIZlsClGXBaLO_4LC0wNuxTswFvOPCMVM6IKPMYA5ds-omjyiZo8o_BF17xF1h6Q3f859pPw2BQLez4Af0HoTO4urgiOMog5vJOMCb3Tqof5_9NImPTlm6UeXkMpnakS420BQOz8Ghzv_V_-_ANdA6p8</recordid><startdate>20210701</startdate><enddate>20210701</enddate><creator>Zhao, Shiyi</creator><creator>Li, Rui</creator><creator>Li, Huiming</creator><creator>Wang, Sa</creator><creator>Zhang, Xinxin</creator><creator>Wang, Dan</creator><creator>Guo, Juan</creator><creator>Li, Huihui</creator><creator>Li, Ao</creator><creator>Tong, Tingting</creator><creator>Zhong, Haixing</creator><creator>Yang, Qianzi</creator><creator>Dong, Hailong</creator><general>Springer Singapore</general><general>Springer Nature</general><general>Springer</general><scope>C6C</scope><scope>BLEPL</scope><scope>DTL</scope><scope>HGBXW</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>5PM</scope><orcidid>https://orcid.org/0000-0001-8984-9067</orcidid></search><sort><creationdate>20210701</creationdate><title>Lateral Hypothalamic Area Glutamatergic Neurons and Their Projections to the Lateral Habenula Modulate the Anesthetic Potency of Isoflurane in Mice</title><author>Zhao, Shiyi ; Li, Rui ; Li, Huiming ; Wang, Sa ; Zhang, Xinxin ; Wang, Dan ; Guo, Juan ; Li, Huihui ; Li, Ao ; Tong, Tingting ; Zhong, Haixing ; Yang, Qianzi ; Dong, Hailong</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c513t-19a609051f1de5616e6f1cc697021481bbd85859d55ef78bf85d50e75fafef03</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2021</creationdate><topic>Anatomy</topic><topic>Anesthesia</topic><topic>Anesthesiology</topic><topic>Biomedical and Life Sciences</topic><topic>Biomedicine</topic><topic>Brain</topic><topic>Electroencephalography</topic><topic>GABA</topic><topic>Human Physiology</topic><topic>Isoflurane</topic><topic>Life Sciences & Biomedicine</topic><topic>Luteinizing hormone</topic><topic>Neurology</topic><topic>Neurons</topic><topic>Neurosciences</topic><topic>Neurosciences & Neurology</topic><topic>Original</topic><topic>Original Article</topic><topic>Pain Medicine</topic><topic>Science & Technology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Zhao, Shiyi</creatorcontrib><creatorcontrib>Li, Rui</creatorcontrib><creatorcontrib>Li, Huiming</creatorcontrib><creatorcontrib>Wang, Sa</creatorcontrib><creatorcontrib>Zhang, Xinxin</creatorcontrib><creatorcontrib>Wang, Dan</creatorcontrib><creatorcontrib>Guo, Juan</creatorcontrib><creatorcontrib>Li, Huihui</creatorcontrib><creatorcontrib>Li, Ao</creatorcontrib><creatorcontrib>Tong, Tingting</creatorcontrib><creatorcontrib>Zhong, Haixing</creatorcontrib><creatorcontrib>Yang, Qianzi</creatorcontrib><creatorcontrib>Dong, Hailong</creatorcontrib><collection>Springer Nature OA Free Journals</collection><collection>Web of Science Core Collection</collection><collection>Science Citation Index Expanded</collection><collection>Web of Science - Science Citation Index Expanded - 2021</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Neuroscience bulletin</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Zhao, Shiyi</au><au>Li, Rui</au><au>Li, Huiming</au><au>Wang, Sa</au><au>Zhang, Xinxin</au><au>Wang, Dan</au><au>Guo, Juan</au><au>Li, Huihui</au><au>Li, Ao</au><au>Tong, Tingting</au><au>Zhong, Haixing</au><au>Yang, Qianzi</au><au>Dong, Hailong</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Lateral Hypothalamic Area Glutamatergic Neurons and Their Projections to the Lateral Habenula Modulate the Anesthetic Potency of Isoflurane in Mice</atitle><jtitle>Neuroscience bulletin</jtitle><stitle>Neurosci. Bull</stitle><stitle>NEUROSCI BULL</stitle><addtitle>Neurosci Bull</addtitle><date>2021-07-01</date><risdate>2021</risdate><volume>37</volume><issue>7</issue><spage>934</spage><epage>946</epage><pages>934-946</pages><issn>1673-7067</issn><eissn>1995-8218</eissn><abstract>The lateral hypothalamic area (LHA) plays a pivotal role in regulating consciousness transition, in which orexinergic neurons, GABAergic neurons, and melanin-concentrating hormone neurons are involved. Glutamatergic neurons have a large population in the LHA, but their anesthesia-related effect has not been explored. Here, we found that genetic ablation of LHA glutamatergic neurons shortened the induction time and prolonged the recovery time of isoflurane anesthesia in mice. In contrast, chemogenetic activation of LHA glutamatergic neurons increased the time to anesthesia and decreased the time to recovery. Optogenetic activation of LHA glutamatergic neurons during the maintenance of anesthesia reduced the burst suppression pattern of the electroencephalogram (EEG) and shifted EEG features to an arousal pattern. Photostimulation of LHA glutamatergic projections to the lateral habenula (LHb) also facilitated the emergence from anesthesia and the transition of anesthesia depth to a lighter level. Collectively, LHA glutamatergic neurons and their projections to the LHb regulate anesthetic potency and EEG features.</abstract><cop>Singapore</cop><pub>Springer Singapore</pub><pmid>33847915</pmid><doi>10.1007/s12264-021-00674-z</doi><tpages>13</tpages><orcidid>https://orcid.org/0000-0001-8984-9067</orcidid><oa>free_for_read</oa></addata></record> |
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| subjects | Anatomy Anesthesia Anesthesiology Biomedical and Life Sciences Biomedicine Brain Electroencephalography GABA Human Physiology Isoflurane Life Sciences & Biomedicine Luteinizing hormone Neurology Neurons Neurosciences Neurosciences & Neurology Original Original Article Pain Medicine Science & Technology |
| title | Lateral Hypothalamic Area Glutamatergic Neurons and Their Projections to the Lateral Habenula Modulate the Anesthetic Potency of Isoflurane in Mice |
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