Slow anion channel GhSLAC1 is essential for stomatal closure in response to drought stress in cotton
•Drought is one of the abiotic stresses which affects the growth and development of cotton.•GhSLAC1, as an S-type anion channel, is an essential element for stomatal closure in response to drought in cotton.•GhSLAC1 has conservative functional amino acid sites. Drought is one of the abiotic stresses...
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| Veröffentlicht in: | Journal of plant physiology 2021-03, Vol.258-259, p.153360-153360, Article 153360 |
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| container_title | Journal of plant physiology |
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| creator | Ren, Huimin Su, Quansheng Hussain, Jamshaid Tang, Shouwu Song, Wu Sun, Yuqiang Liu, Haifeng Qi, Guoning |
| description | •Drought is one of the abiotic stresses which affects the growth and development of cotton.•GhSLAC1, as an S-type anion channel, is an essential element for stomatal closure in response to drought in cotton.•GhSLAC1 has conservative functional amino acid sites.
Drought is one of the abiotic stresses which affects the growth and development of plants, including cotton. The role of stomatal anion channel SLAC1 has been well established in regulating stomatal closure in response to drought stress in several plant species. However, the gene encoding for the main S-type anion channel SLAC1 in cotton has not been identified hence its role in drought stress response remains uncharacterized. In this study, we identified Gh_A08G1582 as the gene encoding for GhSLAC1 in cotton. The gene exhibited abundant expression in leaves and was localized in cell membrane. Furthermore, the expression of GhSLAC1 in Arabidopsis slac1-3 mutants rescued the defective stomatal movement phenotypes of the mutants, pointing to its role in stomata regulation. GhSLAC1 channel was activated by AtOST1 in Xenopus laevis oocytes and showed greater permeability for nitrate than chloride. Further data demonstrated that transgenic cotton lines with silenced GhSLAC1 exhibited obvious leaf wilting phenotype and strong stomatal closure insensitivity under drought stress. Taken together, these results demonstrate that GhSLAC1 is an essential element for stomatal closure in response to drought in cotton. |
| doi_str_mv | 10.1016/j.jplph.2020.153360 |
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Drought is one of the abiotic stresses which affects the growth and development of plants, including cotton. The role of stomatal anion channel SLAC1 has been well established in regulating stomatal closure in response to drought stress in several plant species. However, the gene encoding for the main S-type anion channel SLAC1 in cotton has not been identified hence its role in drought stress response remains uncharacterized. In this study, we identified Gh_A08G1582 as the gene encoding for GhSLAC1 in cotton. The gene exhibited abundant expression in leaves and was localized in cell membrane. Furthermore, the expression of GhSLAC1 in Arabidopsis slac1-3 mutants rescued the defective stomatal movement phenotypes of the mutants, pointing to its role in stomata regulation. GhSLAC1 channel was activated by AtOST1 in Xenopus laevis oocytes and showed greater permeability for nitrate than chloride. Further data demonstrated that transgenic cotton lines with silenced GhSLAC1 exhibited obvious leaf wilting phenotype and strong stomatal closure insensitivity under drought stress. Taken together, these results demonstrate that GhSLAC1 is an essential element for stomatal closure in response to drought in cotton.</description><identifier>ISSN: 0176-1617</identifier><identifier>EISSN: 1618-1328</identifier><identifier>DOI: 10.1016/j.jplph.2020.153360</identifier><identifier>PMID: 33482420</identifier><language>eng</language><publisher>Germany: Elsevier GmbH</publisher><subject>Anions ; Cell membranes ; Cotton ; Drought ; Drought stress ; G. hirsutum L ; Gametocytes ; Gene expression ; GhSLAC1 ; Ion channels ; Leaves ; Life Sciences & Biomedicine ; Mutants ; Oocytes ; Permeability ; Phenotypes ; Plant Sciences ; Plant species ; S-type anion channel ; Science & Technology ; Stomata ; Stomatal closure ; Wilting</subject><ispartof>Journal of plant physiology, 2021-03, Vol.258-259, p.153360-153360, Article 153360</ispartof><rights>2021 Elsevier GmbH</rights><rights>Copyright © 2021 Elsevier GmbH. All rights reserved.</rights><rights>Copyright Urban & Fischer Verlag Mar/Apr 2021</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>true</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c3385-aa5f742744aa31a502767d3e6ccb9de35ea5b828dd04c469bbe5c2fd1dcdbb453</citedby><cites>FETCH-LOGICAL-c3385-aa5f742744aa31a502767d3e6ccb9de35ea5b828dd04c469bbe5c2fd1dcdbb453</cites><orcidid>0000-0003-2067-1381</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.jplph.2020.153360$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>315,781,785,3551,27929,27930,39263,46000</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/33482420$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Ren, Huimin</creatorcontrib><creatorcontrib>Su, Quansheng</creatorcontrib><creatorcontrib>Hussain, Jamshaid</creatorcontrib><creatorcontrib>Tang, Shouwu</creatorcontrib><creatorcontrib>Song, Wu</creatorcontrib><creatorcontrib>Sun, Yuqiang</creatorcontrib><creatorcontrib>Liu, Haifeng</creatorcontrib><creatorcontrib>Qi, Guoning</creatorcontrib><title>Slow anion channel GhSLAC1 is essential for stomatal closure in response to drought stress in cotton</title><title>Journal of plant physiology</title><addtitle>J PLANT PHYSIOL</addtitle><addtitle>J Plant Physiol</addtitle><description>•Drought is one of the abiotic stresses which affects the growth and development of cotton.•GhSLAC1, as an S-type anion channel, is an essential element for stomatal closure in response to drought in cotton.•GhSLAC1 has conservative functional amino acid sites.
Drought is one of the abiotic stresses which affects the growth and development of plants, including cotton. The role of stomatal anion channel SLAC1 has been well established in regulating stomatal closure in response to drought stress in several plant species. However, the gene encoding for the main S-type anion channel SLAC1 in cotton has not been identified hence its role in drought stress response remains uncharacterized. In this study, we identified Gh_A08G1582 as the gene encoding for GhSLAC1 in cotton. The gene exhibited abundant expression in leaves and was localized in cell membrane. Furthermore, the expression of GhSLAC1 in Arabidopsis slac1-3 mutants rescued the defective stomatal movement phenotypes of the mutants, pointing to its role in stomata regulation. GhSLAC1 channel was activated by AtOST1 in Xenopus laevis oocytes and showed greater permeability for nitrate than chloride. Further data demonstrated that transgenic cotton lines with silenced GhSLAC1 exhibited obvious leaf wilting phenotype and strong stomatal closure insensitivity under drought stress. Taken together, these results demonstrate that GhSLAC1 is an essential element for stomatal closure in response to drought in cotton.</description><subject>Anions</subject><subject>Cell membranes</subject><subject>Cotton</subject><subject>Drought</subject><subject>Drought stress</subject><subject>G. hirsutum L</subject><subject>Gametocytes</subject><subject>Gene expression</subject><subject>GhSLAC1</subject><subject>Ion channels</subject><subject>Leaves</subject><subject>Life Sciences & Biomedicine</subject><subject>Mutants</subject><subject>Oocytes</subject><subject>Permeability</subject><subject>Phenotypes</subject><subject>Plant Sciences</subject><subject>Plant species</subject><subject>S-type anion channel</subject><subject>Science & Technology</subject><subject>Stomata</subject><subject>Stomatal closure</subject><subject>Wilting</subject><issn>0176-1617</issn><issn>1618-1328</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2021</creationdate><recordtype>article</recordtype><sourceid>HGBXW</sourceid><recordid>eNp9kc2KFDEUhYMoTjv6BIIE3AhSbf6reuFiaHQUGlyMrkMquWWnqE7KJOXg25u22lm4cJXck-8eDjkIvaRkSwlV78btOE_zccsIq4rkXJFHaEMV7RrKWfcYbQhtVVOF9go9y3kkdZYdf4quOBcdE4xskLub4j02wceA7dGEABO-Pd4dbvYU-4whZwjFmwkPMeFc4smUOtgp5iUB9gEnyHMMGXCJ2KW4fD-WylU1n19tLCWG5-jJYKYMLy7nNfr28cPX_afm8OX28_7m0FjOO9kYI4dWsFYIYzg1krBWtY6DsrbfOeASjOw71jlHhBVq1_cgLRscddb1vZD8Gr1ZfecUfyyQiz75bGGaTIC4ZM1EVz05o21FX_-DjnFJoabTTArB1E4pXim-UjbFnBMMek7-ZNIvTYk-l6BH_acEfS5BryXUrbfr1j30ccjWQ7DwsEkIqc6MSVpv5Bz61SXJ0p_APXB_O6rA-xWA-nU_PSR9sXQ-gS3aRf_fOL8BNVWm3A</recordid><startdate>202103</startdate><enddate>202103</enddate><creator>Ren, Huimin</creator><creator>Su, Quansheng</creator><creator>Hussain, Jamshaid</creator><creator>Tang, Shouwu</creator><creator>Song, Wu</creator><creator>Sun, Yuqiang</creator><creator>Liu, Haifeng</creator><creator>Qi, Guoning</creator><general>Elsevier GmbH</general><general>Elsevier</general><general>Elsevier Science Ltd</general><scope>NPM</scope><scope>BLEPL</scope><scope>DTL</scope><scope>HGBXW</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QP</scope><scope>7SS</scope><scope>8FD</scope><scope>FR3</scope><scope>K9.</scope><scope>M7N</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0003-2067-1381</orcidid></search><sort><creationdate>202103</creationdate><title>Slow anion channel GhSLAC1 is essential for stomatal closure in response to drought stress in cotton</title><author>Ren, Huimin ; Su, Quansheng ; Hussain, Jamshaid ; Tang, Shouwu ; Song, Wu ; Sun, Yuqiang ; Liu, Haifeng ; Qi, Guoning</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c3385-aa5f742744aa31a502767d3e6ccb9de35ea5b828dd04c469bbe5c2fd1dcdbb453</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2021</creationdate><topic>Anions</topic><topic>Cell membranes</topic><topic>Cotton</topic><topic>Drought</topic><topic>Drought stress</topic><topic>G. hirsutum L</topic><topic>Gametocytes</topic><topic>Gene expression</topic><topic>GhSLAC1</topic><topic>Ion channels</topic><topic>Leaves</topic><topic>Life Sciences & Biomedicine</topic><topic>Mutants</topic><topic>Oocytes</topic><topic>Permeability</topic><topic>Phenotypes</topic><topic>Plant Sciences</topic><topic>Plant species</topic><topic>S-type anion channel</topic><topic>Science & Technology</topic><topic>Stomata</topic><topic>Stomatal closure</topic><topic>Wilting</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Ren, Huimin</creatorcontrib><creatorcontrib>Su, Quansheng</creatorcontrib><creatorcontrib>Hussain, Jamshaid</creatorcontrib><creatorcontrib>Tang, Shouwu</creatorcontrib><creatorcontrib>Song, Wu</creatorcontrib><creatorcontrib>Sun, Yuqiang</creatorcontrib><creatorcontrib>Liu, Haifeng</creatorcontrib><creatorcontrib>Qi, Guoning</creatorcontrib><collection>PubMed</collection><collection>Web of Science Core Collection</collection><collection>Science Citation Index Expanded</collection><collection>Web of Science - Science Citation Index Expanded - 2021</collection><collection>CrossRef</collection><collection>Calcium & Calcified Tissue Abstracts</collection><collection>Entomology Abstracts (Full archive)</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of plant physiology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Ren, Huimin</au><au>Su, Quansheng</au><au>Hussain, Jamshaid</au><au>Tang, Shouwu</au><au>Song, Wu</au><au>Sun, Yuqiang</au><au>Liu, Haifeng</au><au>Qi, Guoning</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Slow anion channel GhSLAC1 is essential for stomatal closure in response to drought stress in cotton</atitle><jtitle>Journal of plant physiology</jtitle><stitle>J PLANT PHYSIOL</stitle><addtitle>J Plant Physiol</addtitle><date>2021-03</date><risdate>2021</risdate><volume>258-259</volume><spage>153360</spage><epage>153360</epage><pages>153360-153360</pages><artnum>153360</artnum><issn>0176-1617</issn><eissn>1618-1328</eissn><abstract>•Drought is one of the abiotic stresses which affects the growth and development of cotton.•GhSLAC1, as an S-type anion channel, is an essential element for stomatal closure in response to drought in cotton.•GhSLAC1 has conservative functional amino acid sites.
Drought is one of the abiotic stresses which affects the growth and development of plants, including cotton. The role of stomatal anion channel SLAC1 has been well established in regulating stomatal closure in response to drought stress in several plant species. However, the gene encoding for the main S-type anion channel SLAC1 in cotton has not been identified hence its role in drought stress response remains uncharacterized. In this study, we identified Gh_A08G1582 as the gene encoding for GhSLAC1 in cotton. The gene exhibited abundant expression in leaves and was localized in cell membrane. Furthermore, the expression of GhSLAC1 in Arabidopsis slac1-3 mutants rescued the defective stomatal movement phenotypes of the mutants, pointing to its role in stomata regulation. GhSLAC1 channel was activated by AtOST1 in Xenopus laevis oocytes and showed greater permeability for nitrate than chloride. Further data demonstrated that transgenic cotton lines with silenced GhSLAC1 exhibited obvious leaf wilting phenotype and strong stomatal closure insensitivity under drought stress. Taken together, these results demonstrate that GhSLAC1 is an essential element for stomatal closure in response to drought in cotton.</abstract><cop>Germany</cop><pub>Elsevier GmbH</pub><pmid>33482420</pmid><doi>10.1016/j.jplph.2020.153360</doi><tpages>9</tpages><orcidid>https://orcid.org/0000-0003-2067-1381</orcidid></addata></record> |
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| subjects | Anions Cell membranes Cotton Drought Drought stress G. hirsutum L Gametocytes Gene expression GhSLAC1 Ion channels Leaves Life Sciences & Biomedicine Mutants Oocytes Permeability Phenotypes Plant Sciences Plant species S-type anion channel Science & Technology Stomata Stomatal closure Wilting |
| title | Slow anion channel GhSLAC1 is essential for stomatal closure in response to drought stress in cotton |
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