The synthesis of a UCST-type zwitterionic polymer for the efficient recycling of cellulase at room temperature
In order to reduce the enzyme cost of lignocellulosic enzymatic hydrolysis, upper critical solution temperature (UCST) additives are used to recover and reuse cellulase by regulating the temperature. A sulfobetaine polymer, PSPE, showed a sensitive UCST response in buffer, and its UCST-performance w...
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| Veröffentlicht in: | Green chemistry : an international journal and green chemistry resource : GC 2021-04, Vol.23 (7), p.2738-2746 |
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| container_title | Green chemistry : an international journal and green chemistry resource : GC |
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| creator | Li, Feiyun Qin, Feiyang Pang, Yuxia Lou, Hongming Cai, Cheng Liu, Weifeng Qian, Yong Qiu, Xueqing |
| description | In order to reduce the enzyme cost of lignocellulosic enzymatic hydrolysis, upper critical solution temperature (UCST) additives are used to recover and reuse cellulase by regulating the temperature. A sulfobetaine polymer, PSPE, showed a sensitive UCST response in buffer, and its UCST-performance was optimised by adjusting the molecular weight of the polymer. PSPE-3 (
M
w
= 355.1 kDa) was added to the cellulase buffer at 50 °C, and PSPE-3 and cellulase were co-precipitated by cooling to the room temperature of 25 °C. PSPE-3 and cellulase co-precipitated mainly through hydrophobic interactions
via
cooling. Compared with octadecyl sulfobetaine (SB3-18), PSPE-3 had a more sensitive UCST response and stronger cellulase recovery ability. Adding only 40% PSPE-3 (accounting for the mass fraction of cellulase) can save more than 50% cellulase in the enzymatic hydrolysis system of eucalyptus chips pre-treated with dilute acid (Eu-DA). However, for the Eu-DA system, adding 100% SB3-18 can only save about 30% of cellulase. In this work, efficient recycling of cellulase was achieved at room temperature by adding UCST-type PSPE. The process is green without consuming acid and alkali, and this work provides a new idea for reducing the production cost of cellulosic ethanol.
Cellulase was recycled by adding UCST-type PSPE in lignocellulosic biorefineries, and the process is sustainable without consuming acid and alkali. |
| doi_str_mv | 10.1039/d0gc04333h |
| format | Article |
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M
w
= 355.1 kDa) was added to the cellulase buffer at 50 °C, and PSPE-3 and cellulase were co-precipitated by cooling to the room temperature of 25 °C. PSPE-3 and cellulase co-precipitated mainly through hydrophobic interactions
via
cooling. Compared with octadecyl sulfobetaine (SB3-18), PSPE-3 had a more sensitive UCST response and stronger cellulase recovery ability. Adding only 40% PSPE-3 (accounting for the mass fraction of cellulase) can save more than 50% cellulase in the enzymatic hydrolysis system of eucalyptus chips pre-treated with dilute acid (Eu-DA). However, for the Eu-DA system, adding 100% SB3-18 can only save about 30% of cellulase. In this work, efficient recycling of cellulase was achieved at room temperature by adding UCST-type PSPE. The process is green without consuming acid and alkali, and this work provides a new idea for reducing the production cost of cellulosic ethanol.
Cellulase was recycled by adding UCST-type PSPE in lignocellulosic biorefineries, and the process is sustainable without consuming acid and alkali.</description><identifier>ISSN: 1463-9262</identifier><identifier>EISSN: 1463-9270</identifier><identifier>DOI: 10.1039/d0gc04333h</identifier><language>eng</language><publisher>CAMBRIDGE: Royal Soc Chemistry</publisher><subject>Additives ; Buffers ; Cellulase ; Chemical synthesis ; Chemistry ; Chemistry, Multidisciplinary ; Cooling ; Ethanol ; Eucalyptus ; Green & Sustainable Science & Technology ; Green chemistry ; Hydrolysis ; Hydrophobicity ; Lignocellulose ; Molecular weight ; Physical Sciences ; Polymers ; Production costs ; Recycling ; Room temperature ; Science & Technology ; Science & Technology - Other Topics ; Zwitterions</subject><ispartof>Green chemistry : an international journal and green chemistry resource : GC, 2021-04, Vol.23 (7), p.2738-2746</ispartof><rights>Copyright Royal Society of Chemistry 2021</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>true</woscitedreferencessubscribed><woscitedreferencescount>13</woscitedreferencescount><woscitedreferencesoriginalsourcerecordid>wos000632134800001</woscitedreferencesoriginalsourcerecordid><citedby>FETCH-LOGICAL-c318t-201c9b2b84298a6a2ba6355b5843bfe307f31010632d4ea2e3167d0c24672c863</citedby><cites>FETCH-LOGICAL-c318t-201c9b2b84298a6a2ba6355b5843bfe307f31010632d4ea2e3167d0c24672c863</cites><orcidid>0000-0001-8765-7061 ; 0000-0003-3941-7287</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>315,781,785,27929,27930,39263</link.rule.ids></links><search><creatorcontrib>Li, Feiyun</creatorcontrib><creatorcontrib>Qin, Feiyang</creatorcontrib><creatorcontrib>Pang, Yuxia</creatorcontrib><creatorcontrib>Lou, Hongming</creatorcontrib><creatorcontrib>Cai, Cheng</creatorcontrib><creatorcontrib>Liu, Weifeng</creatorcontrib><creatorcontrib>Qian, Yong</creatorcontrib><creatorcontrib>Qiu, Xueqing</creatorcontrib><title>The synthesis of a UCST-type zwitterionic polymer for the efficient recycling of cellulase at room temperature</title><title>Green chemistry : an international journal and green chemistry resource : GC</title><addtitle>GREEN CHEM</addtitle><description>In order to reduce the enzyme cost of lignocellulosic enzymatic hydrolysis, upper critical solution temperature (UCST) additives are used to recover and reuse cellulase by regulating the temperature. A sulfobetaine polymer, PSPE, showed a sensitive UCST response in buffer, and its UCST-performance was optimised by adjusting the molecular weight of the polymer. PSPE-3 (
M
w
= 355.1 kDa) was added to the cellulase buffer at 50 °C, and PSPE-3 and cellulase were co-precipitated by cooling to the room temperature of 25 °C. PSPE-3 and cellulase co-precipitated mainly through hydrophobic interactions
via
cooling. Compared with octadecyl sulfobetaine (SB3-18), PSPE-3 had a more sensitive UCST response and stronger cellulase recovery ability. Adding only 40% PSPE-3 (accounting for the mass fraction of cellulase) can save more than 50% cellulase in the enzymatic hydrolysis system of eucalyptus chips pre-treated with dilute acid (Eu-DA). However, for the Eu-DA system, adding 100% SB3-18 can only save about 30% of cellulase. In this work, efficient recycling of cellulase was achieved at room temperature by adding UCST-type PSPE. The process is green without consuming acid and alkali, and this work provides a new idea for reducing the production cost of cellulosic ethanol.
Cellulase was recycled by adding UCST-type PSPE in lignocellulosic biorefineries, and the process is sustainable without consuming acid and alkali.</description><subject>Additives</subject><subject>Buffers</subject><subject>Cellulase</subject><subject>Chemical synthesis</subject><subject>Chemistry</subject><subject>Chemistry, Multidisciplinary</subject><subject>Cooling</subject><subject>Ethanol</subject><subject>Eucalyptus</subject><subject>Green & Sustainable Science & Technology</subject><subject>Green chemistry</subject><subject>Hydrolysis</subject><subject>Hydrophobicity</subject><subject>Lignocellulose</subject><subject>Molecular weight</subject><subject>Physical Sciences</subject><subject>Polymers</subject><subject>Production costs</subject><subject>Recycling</subject><subject>Room temperature</subject><subject>Science & Technology</subject><subject>Science & Technology - Other Topics</subject><subject>Zwitterions</subject><issn>1463-9262</issn><issn>1463-9270</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2021</creationdate><recordtype>article</recordtype><sourceid>HGBXW</sourceid><recordid>eNqNkctr3DAQxk1JoHn00ntBkFuD0xnJK9vH4jwhkEM3ZyNrR1kFr-VIMsH566vNhs01pxmY3zePb7LsJ8IFgqj_rOBJQyGEWH_LjrCQIq95CQf7XPLv2XEIzwCIpSyOsmG5JhbmIa4p2MCcYYo9Nv-WeZxHYm-vNkby1g1Ws9H184Y8M86zhDMyxmpLQ2Se9Kx7Ozxt9Zr6fupVIKZSxbkNi7QZyas4eTrNDo3qA_34iCfZ4_XVsrnN7x9u7pq_97kWWMWcA-q6411V8LpSUvFOSbFYdIuqEJ0hAaURCAhS8FVBipNAWa5A80KWXFdSnGRnu76jdy8Thdg-u8kPaWTLF4g1Co6YqN87SnsXgifTjt5ulJ9bhHbrZ3sJN827n7cJrnbwK3XOhO3lmvYCgO0yKIoqZYCNjSom2xo3DTFJz78uTfSvHe2D3kOfjxX_AQtRknk</recordid><startdate>20210413</startdate><enddate>20210413</enddate><creator>Li, Feiyun</creator><creator>Qin, Feiyang</creator><creator>Pang, Yuxia</creator><creator>Lou, Hongming</creator><creator>Cai, Cheng</creator><creator>Liu, Weifeng</creator><creator>Qian, Yong</creator><creator>Qiu, Xueqing</creator><general>Royal Soc Chemistry</general><general>Royal Society of Chemistry</general><scope>BLEPL</scope><scope>DTL</scope><scope>HGBXW</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7SR</scope><scope>7ST</scope><scope>7U6</scope><scope>8BQ</scope><scope>8FD</scope><scope>C1K</scope><scope>JG9</scope><orcidid>https://orcid.org/0000-0001-8765-7061</orcidid><orcidid>https://orcid.org/0000-0003-3941-7287</orcidid></search><sort><creationdate>20210413</creationdate><title>The synthesis of a UCST-type zwitterionic polymer for the efficient recycling of cellulase at room temperature</title><author>Li, Feiyun ; Qin, Feiyang ; Pang, Yuxia ; Lou, Hongming ; Cai, Cheng ; Liu, Weifeng ; Qian, Yong ; Qiu, Xueqing</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c318t-201c9b2b84298a6a2ba6355b5843bfe307f31010632d4ea2e3167d0c24672c863</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2021</creationdate><topic>Additives</topic><topic>Buffers</topic><topic>Cellulase</topic><topic>Chemical synthesis</topic><topic>Chemistry</topic><topic>Chemistry, Multidisciplinary</topic><topic>Cooling</topic><topic>Ethanol</topic><topic>Eucalyptus</topic><topic>Green & Sustainable Science & Technology</topic><topic>Green chemistry</topic><topic>Hydrolysis</topic><topic>Hydrophobicity</topic><topic>Lignocellulose</topic><topic>Molecular weight</topic><topic>Physical Sciences</topic><topic>Polymers</topic><topic>Production costs</topic><topic>Recycling</topic><topic>Room temperature</topic><topic>Science & Technology</topic><topic>Science & Technology - Other Topics</topic><topic>Zwitterions</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Li, Feiyun</creatorcontrib><creatorcontrib>Qin, Feiyang</creatorcontrib><creatorcontrib>Pang, Yuxia</creatorcontrib><creatorcontrib>Lou, Hongming</creatorcontrib><creatorcontrib>Cai, Cheng</creatorcontrib><creatorcontrib>Liu, Weifeng</creatorcontrib><creatorcontrib>Qian, Yong</creatorcontrib><creatorcontrib>Qiu, Xueqing</creatorcontrib><collection>Web of Science Core Collection</collection><collection>Science Citation Index Expanded</collection><collection>Web of Science - Science Citation Index Expanded - 2021</collection><collection>CrossRef</collection><collection>Engineered Materials Abstracts</collection><collection>Environment Abstracts</collection><collection>Sustainability Science Abstracts</collection><collection>METADEX</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Materials Research Database</collection><jtitle>Green chemistry : an international journal and green chemistry resource : GC</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Li, Feiyun</au><au>Qin, Feiyang</au><au>Pang, Yuxia</au><au>Lou, Hongming</au><au>Cai, Cheng</au><au>Liu, Weifeng</au><au>Qian, Yong</au><au>Qiu, Xueqing</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>The synthesis of a UCST-type zwitterionic polymer for the efficient recycling of cellulase at room temperature</atitle><jtitle>Green chemistry : an international journal and green chemistry resource : GC</jtitle><stitle>GREEN CHEM</stitle><date>2021-04-13</date><risdate>2021</risdate><volume>23</volume><issue>7</issue><spage>2738</spage><epage>2746</epage><pages>2738-2746</pages><issn>1463-9262</issn><eissn>1463-9270</eissn><abstract>In order to reduce the enzyme cost of lignocellulosic enzymatic hydrolysis, upper critical solution temperature (UCST) additives are used to recover and reuse cellulase by regulating the temperature. A sulfobetaine polymer, PSPE, showed a sensitive UCST response in buffer, and its UCST-performance was optimised by adjusting the molecular weight of the polymer. PSPE-3 (
M
w
= 355.1 kDa) was added to the cellulase buffer at 50 °C, and PSPE-3 and cellulase were co-precipitated by cooling to the room temperature of 25 °C. PSPE-3 and cellulase co-precipitated mainly through hydrophobic interactions
via
cooling. Compared with octadecyl sulfobetaine (SB3-18), PSPE-3 had a more sensitive UCST response and stronger cellulase recovery ability. Adding only 40% PSPE-3 (accounting for the mass fraction of cellulase) can save more than 50% cellulase in the enzymatic hydrolysis system of eucalyptus chips pre-treated with dilute acid (Eu-DA). However, for the Eu-DA system, adding 100% SB3-18 can only save about 30% of cellulase. In this work, efficient recycling of cellulase was achieved at room temperature by adding UCST-type PSPE. The process is green without consuming acid and alkali, and this work provides a new idea for reducing the production cost of cellulosic ethanol.
Cellulase was recycled by adding UCST-type PSPE in lignocellulosic biorefineries, and the process is sustainable without consuming acid and alkali.</abstract><cop>CAMBRIDGE</cop><pub>Royal Soc Chemistry</pub><doi>10.1039/d0gc04333h</doi><tpages>9</tpages><orcidid>https://orcid.org/0000-0001-8765-7061</orcidid><orcidid>https://orcid.org/0000-0003-3941-7287</orcidid></addata></record> |
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| ispartof | Green chemistry : an international journal and green chemistry resource : GC, 2021-04, Vol.23 (7), p.2738-2746 |
| issn | 1463-9262 1463-9270 |
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| source | Royal Society Of Chemistry Journals; Web of Science - Science Citation Index Expanded - 2021<img src="https://exlibris-pub.s3.amazonaws.com/fromwos-v2.jpg" />; Alma/SFX Local Collection |
| subjects | Additives Buffers Cellulase Chemical synthesis Chemistry Chemistry, Multidisciplinary Cooling Ethanol Eucalyptus Green & Sustainable Science & Technology Green chemistry Hydrolysis Hydrophobicity Lignocellulose Molecular weight Physical Sciences Polymers Production costs Recycling Room temperature Science & Technology Science & Technology - Other Topics Zwitterions |
| title | The synthesis of a UCST-type zwitterionic polymer for the efficient recycling of cellulase at room temperature |
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