A simple carrot agar medium for isolation of black root rot pathogen of cotton seedlings
Black root rot (BRR) is a major seedling disease in cotton in Australia. BRR is caused by a soilborne fungus Thielaviopsis basicola , recently re-described as Berkelyomyces spp., that was reported for the first time in 1990 in northern New South Wales (NSW), Australia. The disease is now prevalent a...
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| Veröffentlicht in: | Australasian plant pathology 2021, Vol.50 (3), p.319-322 |
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| description | Black root rot (BRR) is a major seedling disease in cotton in Australia. BRR is caused by a soilborne fungus
Thielaviopsis basicola
, recently re-described as
Berkelyomyces
spp., that was reported for the first time in 1990 in northern New South Wales (NSW), Australia. The disease is now prevalent across NSW. Since the first detection, much research has been focused exclusively on management; however, little has been investigated to understand the BRR pathogen population. Isolation and collection of pure fungal cultures are essential for investigation of the pathogen diversity and pathogenicity. However, isolation of
T. basicola
is recalcitrant. In this study,
T. basicola
were successfully recovered from BRR diseased cotton seedlings in the past three seasons by using a simple 5% carrot agar amended with 100 ppm streptomycin.
T. basicola
was recovered within three days with the percentage of recovery ranging from 55–76% during the first isolation attempt. This carrot medium provided a simple and vigorous means for isolation of
T. basicola
. |
| doi_str_mv | 10.1007/s13313-021-00775-3 |
| format | Article |
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Thielaviopsis basicola
, recently re-described as
Berkelyomyces
spp., that was reported for the first time in 1990 in northern New South Wales (NSW), Australia. The disease is now prevalent across NSW. Since the first detection, much research has been focused exclusively on management; however, little has been investigated to understand the BRR pathogen population. Isolation and collection of pure fungal cultures are essential for investigation of the pathogen diversity and pathogenicity. However, isolation of
T. basicola
is recalcitrant. In this study,
T. basicola
were successfully recovered from BRR diseased cotton seedlings in the past three seasons by using a simple 5% carrot agar amended with 100 ppm streptomycin.
T. basicola
was recovered within three days with the percentage of recovery ranging from 55–76% during the first isolation attempt. This carrot medium provided a simple and vigorous means for isolation of
T. basicola
.</description><identifier>ISSN: 0815-3191</identifier><identifier>EISSN: 1448-6032</identifier><identifier>DOI: 10.1007/s13313-021-00775-3</identifier><language>eng</language><publisher>Dordrecht: Springer Netherlands</publisher><subject>Agriculture ; Biomedical and Life Sciences ; Black root ; Cotton ; Ecology ; Entomology ; Life Sciences ; Life Sciences & Biomedicine ; Pathogenicity ; Pathogens ; Plant Pathology ; Plant Sciences ; Research Note ; Root rot ; Science & Technology ; Seedlings ; Streptomycin</subject><ispartof>Australasian plant pathology, 2021, Vol.50 (3), p.319-322</ispartof><rights>Crown 2021</rights><rights>Crown 2021.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>true</woscitedreferencessubscribed><woscitedreferencescount>2</woscitedreferencescount><woscitedreferencesoriginalsourcerecordid>wos000618131400001</woscitedreferencesoriginalsourcerecordid><citedby>FETCH-LOGICAL-c319t-12b4a105392d4fb41047e04ca167855863f288c5581091e25eb453c09dab3ee13</citedby><cites>FETCH-LOGICAL-c319t-12b4a105392d4fb41047e04ca167855863f288c5581091e25eb453c09dab3ee13</cites><orcidid>0000-0003-3331-1342</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s13313-021-00775-3$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s13313-021-00775-3$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>315,781,785,27929,27930,39263,41493,42562,51324</link.rule.ids></links><search><creatorcontrib>Le, Duy Phu</creatorcontrib><title>A simple carrot agar medium for isolation of black root rot pathogen of cotton seedlings</title><title>Australasian plant pathology</title><addtitle>Australasian Plant Pathol</addtitle><addtitle>AUSTRALAS PLANT PATH</addtitle><description>Black root rot (BRR) is a major seedling disease in cotton in Australia. BRR is caused by a soilborne fungus
Thielaviopsis basicola
, recently re-described as
Berkelyomyces
spp., that was reported for the first time in 1990 in northern New South Wales (NSW), Australia. The disease is now prevalent across NSW. Since the first detection, much research has been focused exclusively on management; however, little has been investigated to understand the BRR pathogen population. Isolation and collection of pure fungal cultures are essential for investigation of the pathogen diversity and pathogenicity. However, isolation of
T. basicola
is recalcitrant. In this study,
T. basicola
were successfully recovered from BRR diseased cotton seedlings in the past three seasons by using a simple 5% carrot agar amended with 100 ppm streptomycin.
T. basicola
was recovered within three days with the percentage of recovery ranging from 55–76% during the first isolation attempt. This carrot medium provided a simple and vigorous means for isolation of
T. basicola
.</description><subject>Agriculture</subject><subject>Biomedical and Life Sciences</subject><subject>Black root</subject><subject>Cotton</subject><subject>Ecology</subject><subject>Entomology</subject><subject>Life Sciences</subject><subject>Life Sciences & Biomedicine</subject><subject>Pathogenicity</subject><subject>Pathogens</subject><subject>Plant Pathology</subject><subject>Plant Sciences</subject><subject>Research Note</subject><subject>Root rot</subject><subject>Science & Technology</subject><subject>Seedlings</subject><subject>Streptomycin</subject><issn>0815-3191</issn><issn>1448-6032</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2021</creationdate><recordtype>article</recordtype><sourceid>HGBXW</sourceid><recordid>eNqNkE9LAzEQxYMoWKtfwFPAo6zObLLb7LEU_0HBi4K3kE1n69btpiYp4rc37YrexFMmmd-bvHmMnSNcIcDkOqAQKDLIMUvXSZGJAzZCKVVWgsgP2QgUpkes8JidhLACQFkKGLGXKQ_tetMRt8Z7F7lZGs_XtGi3a944z9vgOhNb13PX8Loz9o17l7gduzHx1S1p37IuxgQFokXX9stwyo4a0wU6-z7H7Pn25ml2n80f7x5m03lmk5uYYV5Lg1CIKl_IppYIckIgrcFyoopClaLJlbKpQqiQ8oJqWQgL1cLUggjFmF0MczfevW8pRL1yW9-nL3WuKiVFBaVMVD5Q1rsQPDV649u18Z8aQe8S1EOCOiWo9wlqkUSXg-iDatcE21Jv6UcIACUqFChTBTsj6v_0rI37UGdu28ckFYM0JLxfkv_d4Q97XxWrkxE</recordid><startdate>2021</startdate><enddate>2021</enddate><creator>Le, Duy Phu</creator><general>Springer Netherlands</general><general>Springer Nature</general><general>Springer Nature B.V</general><scope>BLEPL</scope><scope>DTL</scope><scope>HGBXW</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>7T7</scope><scope>7U9</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>H94</scope><scope>M7N</scope><scope>P64</scope><orcidid>https://orcid.org/0000-0003-3331-1342</orcidid></search><sort><creationdate>2021</creationdate><title>A simple carrot agar medium for isolation of black root rot pathogen of cotton seedlings</title><author>Le, Duy Phu</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c319t-12b4a105392d4fb41047e04ca167855863f288c5581091e25eb453c09dab3ee13</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2021</creationdate><topic>Agriculture</topic><topic>Biomedical and Life Sciences</topic><topic>Black root</topic><topic>Cotton</topic><topic>Ecology</topic><topic>Entomology</topic><topic>Life Sciences</topic><topic>Life Sciences & Biomedicine</topic><topic>Pathogenicity</topic><topic>Pathogens</topic><topic>Plant Pathology</topic><topic>Plant Sciences</topic><topic>Research Note</topic><topic>Root rot</topic><topic>Science & Technology</topic><topic>Seedlings</topic><topic>Streptomycin</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Le, Duy Phu</creatorcontrib><collection>Web of Science Core Collection</collection><collection>Science Citation Index Expanded</collection><collection>Web of Science - Science Citation Index Expanded - 2021</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Virology and AIDS Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><jtitle>Australasian plant pathology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Le, Duy Phu</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>A simple carrot agar medium for isolation of black root rot pathogen of cotton seedlings</atitle><jtitle>Australasian plant pathology</jtitle><stitle>Australasian Plant Pathol</stitle><stitle>AUSTRALAS PLANT PATH</stitle><date>2021</date><risdate>2021</risdate><volume>50</volume><issue>3</issue><spage>319</spage><epage>322</epage><pages>319-322</pages><issn>0815-3191</issn><eissn>1448-6032</eissn><abstract>Black root rot (BRR) is a major seedling disease in cotton in Australia. BRR is caused by a soilborne fungus
Thielaviopsis basicola
, recently re-described as
Berkelyomyces
spp., that was reported for the first time in 1990 in northern New South Wales (NSW), Australia. The disease is now prevalent across NSW. Since the first detection, much research has been focused exclusively on management; however, little has been investigated to understand the BRR pathogen population. Isolation and collection of pure fungal cultures are essential for investigation of the pathogen diversity and pathogenicity. However, isolation of
T. basicola
is recalcitrant. In this study,
T. basicola
were successfully recovered from BRR diseased cotton seedlings in the past three seasons by using a simple 5% carrot agar amended with 100 ppm streptomycin.
T. basicola
was recovered within three days with the percentage of recovery ranging from 55–76% during the first isolation attempt. This carrot medium provided a simple and vigorous means for isolation of
T. basicola
.</abstract><cop>Dordrecht</cop><pub>Springer Netherlands</pub><doi>10.1007/s13313-021-00775-3</doi><tpages>4</tpages><orcidid>https://orcid.org/0000-0003-3331-1342</orcidid></addata></record> |
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| ispartof | Australasian plant pathology, 2021, Vol.50 (3), p.319-322 |
| issn | 0815-3191 1448-6032 |
| language | eng |
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| source | SpringerNature Journals; Web of Science - Science Citation Index Expanded - 2021<img src="https://exlibris-pub.s3.amazonaws.com/fromwos-v2.jpg" /> |
| subjects | Agriculture Biomedical and Life Sciences Black root Cotton Ecology Entomology Life Sciences Life Sciences & Biomedicine Pathogenicity Pathogens Plant Pathology Plant Sciences Research Note Root rot Science & Technology Seedlings Streptomycin |
| title | A simple carrot agar medium for isolation of black root rot pathogen of cotton seedlings |
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