Decreasing formalin concentration improves quality of DNA extracted from formalin-fixed paraffin-embedded tissue specimens without compromising tissue morphology or immunohistochemical staining

Decreasing formalin concentration improves quality of DNA extracted from formalin-fixed paraffin-embedded tissue specimens without compromising tissue morphology or immunohistochemical staining

Genomic technologies are increasingly used clinically for both diagnosis and guiding cancer therapy. However, formalin fixation can compromise DNA quality. This study aimed to optimise tissue fixation using normal colon, liver and uterus (n=8 each) by varying neutral buffered formalin (NBF) concentr...

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Veröffentlicht in:Journal of clinical pathology 2020-08, Vol.73 (8), p.514-518
Hauptverfasser: Cummings, Michele, King, Henry, Hurst, James, Tanner, Georgette, Khazin, Leah, Thompson, Phillip, Gray, Allan, Gahir, Narinder, Cartlidge, Caroline, Farooq, Zara, Raveendran, Keyura, Allen, Katie, Rotimi, Olorunda, Orsi, Nicolas M
Format: Artikel
Sprache:eng
Schlagworte:
Deoxyribonucleic acid
DNA
Gene expression
immunohistochemistry
Laboratories
Liver
molecular pathology
Morphology
morphometry
PCR
Quality
Short report
Uterus
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Zusammenfassung:Genomic technologies are increasingly used clinically for both diagnosis and guiding cancer therapy. However, formalin fixation can compromise DNA quality. This study aimed to optimise tissue fixation using normal colon, liver and uterus (n=8 each) by varying neutral buffered formalin (NBF) concentration (1%–5% w/v) and fixation time (24–48 hours). Fixation using 4% NBF improved DNA quality (assessed by qPCR) compared with routine (4% unbuffered formal saline-fixed) specimens (p
ISSN:0021-9746
1472-4146
DOI:10.1136/jclinpath-2019-206368