Effects of Cellulolytic Bacteria on Nitrogen-Fixing Bacteria, 16S rRNA, nifH Gene Abundance, and Chemical Properties of Water Hyacinth Compost
This research investigated alterations to and the interdependency of nitrogen-fixing bacteria, 16S ribosomal ribonucleic acid gene (16S rRNA) and nitrogenase reductase gene ( nifH ) gene abundance, and chemical properties of water hyacinth compost when using cellulolytic bacteria isolated from soil...
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| Veröffentlicht in: | Journal of soil science and plant nutrition 2021-03, Vol.21 (1), p.768-779 |
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| creator | Chungopast, Sirinapa Yodying, Preecha Nomura, Mika |
| description | This research investigated alterations to and the interdependency of nitrogen-fixing bacteria, 16S ribosomal ribonucleic acid gene (16S rRNA) and nitrogenase reductase gene (
nifH
) gene abundance, and chemical properties of water hyacinth compost when using cellulolytic bacteria isolated from soil and leaf litter (CSL) inoculum. The un- and inoculated treatments in the compost were designed with three replications. Microbiological analysis involved examination of the total number of bacteria and gene abundance in the compost based on quantitative real-time polymerase chain reaction (qPCR). Some chemical properties of the compost were also analyzed. The results indicated that applying cellulolytic bacteria into compost could increase the amounts of bacteria, especially nitrogen-fixing bacteria. The pH of the compost increased slightly for the first 4 weeks. The amount of nitrogen and organic matter (OM) in the compost increased continuously during the composting period. The concentration of ammonium changed markedly in the range 1.5–2 times at the 4th and 10th weeks of the composting process, which was consistent with an increase of nitrogen-fixing bacteria. The concentration of nitrate doubled at the 12th week. The abundance of 16S rRNA and
nifH
genes was significantly correlated with the number of bacteria, total nitrogen, ammonium, nitrate, and OM. The inoculated cellulolytic bacteria not only accelerated the nitrogen mineralization process but also promoted bacterial numbers in the compost. These bacteria also affected the transformation of nutrients and correlated positively with gene abundance. |
| doi_str_mv | 10.1007/s42729-020-00399-4 |
| format | Article |
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nifH
) gene abundance, and chemical properties of water hyacinth compost when using cellulolytic bacteria isolated from soil and leaf litter (CSL) inoculum. The un- and inoculated treatments in the compost were designed with three replications. Microbiological analysis involved examination of the total number of bacteria and gene abundance in the compost based on quantitative real-time polymerase chain reaction (qPCR). Some chemical properties of the compost were also analyzed. The results indicated that applying cellulolytic bacteria into compost could increase the amounts of bacteria, especially nitrogen-fixing bacteria. The pH of the compost increased slightly for the first 4 weeks. The amount of nitrogen and organic matter (OM) in the compost increased continuously during the composting period. The concentration of ammonium changed markedly in the range 1.5–2 times at the 4th and 10th weeks of the composting process, which was consistent with an increase of nitrogen-fixing bacteria. The concentration of nitrate doubled at the 12th week. The abundance of 16S rRNA and
nifH
genes was significantly correlated with the number of bacteria, total nitrogen, ammonium, nitrate, and OM. The inoculated cellulolytic bacteria not only accelerated the nitrogen mineralization process but also promoted bacterial numbers in the compost. These bacteria also affected the transformation of nutrients and correlated positively with gene abundance.</description><identifier>ISSN: 0718-9508</identifier><identifier>EISSN: 0718-9516</identifier><identifier>DOI: 10.1007/s42729-020-00399-4</identifier><language>eng</language><publisher>Cham: Springer International Publishing</publisher><subject>Abundance ; Agriculture ; Ammonium ; Aquatic plants ; Bacteria ; Biomedical and Life Sciences ; Cellulase ; Cellulolytic bacteria ; Cellulose ; Chemical properties ; Composting ; Composts ; Decomposition ; Deoxyribonucleic acid ; DNA ; Ecology ; Environment ; Floating plants ; Gene amplification ; Glucose ; Inoculum ; Leaf litter ; Life Sciences ; Microbiological analysis ; Microorganisms ; Mineralization ; Molecular biology ; Mosses ; NifH gene ; Nitrogen ; Nitrogen fixation ; Nitrogen-fixing bacteria ; Nitrogenase ; Nitrogenase reductase ; Nitrogenation ; Nutrients ; Organic matter ; Original Paper ; Plant Sciences ; Polymerase chain reaction ; Potassium ; Reductases ; Ribonucleic acid ; RNA ; rRNA 16S ; Soil bacteria ; Soil microorganisms ; Soil Science & Conservation ; Water hyacinths</subject><ispartof>Journal of soil science and plant nutrition, 2021-03, Vol.21 (1), p.768-779</ispartof><rights>Sociedad Chilena de la Ciencia del Suelo 2021</rights><rights>Sociedad Chilena de la Ciencia del Suelo 2021.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c385t-701ab713a1b42c1ac88b2841f421599802553ecc3b0525c0dc29cc12b79f8c263</citedby><cites>FETCH-LOGICAL-c385t-701ab713a1b42c1ac88b2841f421599802553ecc3b0525c0dc29cc12b79f8c263</cites><orcidid>0000-0002-8856-3275</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s42729-020-00399-4$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://www.proquest.com/docview/2932581229?pq-origsite=primo$$EHTML$$P50$$Gproquest$$H</linktohtml><link.rule.ids>314,780,784,21388,21389,21390,21391,23256,27924,27925,33530,33703,33744,34005,34314,41488,42557,43659,43787,43805,43953,44067,51319,64385,64389,72469</link.rule.ids></links><search><creatorcontrib>Chungopast, Sirinapa</creatorcontrib><creatorcontrib>Yodying, Preecha</creatorcontrib><creatorcontrib>Nomura, Mika</creatorcontrib><title>Effects of Cellulolytic Bacteria on Nitrogen-Fixing Bacteria, 16S rRNA, nifH Gene Abundance, and Chemical Properties of Water Hyacinth Compost</title><title>Journal of soil science and plant nutrition</title><addtitle>J Soil Sci Plant Nutr</addtitle><description>This research investigated alterations to and the interdependency of nitrogen-fixing bacteria, 16S ribosomal ribonucleic acid gene (16S rRNA) and nitrogenase reductase gene (
nifH
) gene abundance, and chemical properties of water hyacinth compost when using cellulolytic bacteria isolated from soil and leaf litter (CSL) inoculum. The un- and inoculated treatments in the compost were designed with three replications. Microbiological analysis involved examination of the total number of bacteria and gene abundance in the compost based on quantitative real-time polymerase chain reaction (qPCR). Some chemical properties of the compost were also analyzed. The results indicated that applying cellulolytic bacteria into compost could increase the amounts of bacteria, especially nitrogen-fixing bacteria. The pH of the compost increased slightly for the first 4 weeks. The amount of nitrogen and organic matter (OM) in the compost increased continuously during the composting period. The concentration of ammonium changed markedly in the range 1.5–2 times at the 4th and 10th weeks of the composting process, which was consistent with an increase of nitrogen-fixing bacteria. The concentration of nitrate doubled at the 12th week. The abundance of 16S rRNA and
nifH
genes was significantly correlated with the number of bacteria, total nitrogen, ammonium, nitrate, and OM. The inoculated cellulolytic bacteria not only accelerated the nitrogen mineralization process but also promoted bacterial numbers in the compost. These bacteria also affected the transformation of nutrients and correlated positively with gene abundance.</description><subject>Abundance</subject><subject>Agriculture</subject><subject>Ammonium</subject><subject>Aquatic plants</subject><subject>Bacteria</subject><subject>Biomedical and Life Sciences</subject><subject>Cellulase</subject><subject>Cellulolytic bacteria</subject><subject>Cellulose</subject><subject>Chemical properties</subject><subject>Composting</subject><subject>Composts</subject><subject>Decomposition</subject><subject>Deoxyribonucleic acid</subject><subject>DNA</subject><subject>Ecology</subject><subject>Environment</subject><subject>Floating plants</subject><subject>Gene amplification</subject><subject>Glucose</subject><subject>Inoculum</subject><subject>Leaf litter</subject><subject>Life Sciences</subject><subject>Microbiological analysis</subject><subject>Microorganisms</subject><subject>Mineralization</subject><subject>Molecular biology</subject><subject>Mosses</subject><subject>NifH gene</subject><subject>Nitrogen</subject><subject>Nitrogen fixation</subject><subject>Nitrogen-fixing bacteria</subject><subject>Nitrogenase</subject><subject>Nitrogenase reductase</subject><subject>Nitrogenation</subject><subject>Nutrients</subject><subject>Organic matter</subject><subject>Original Paper</subject><subject>Plant Sciences</subject><subject>Polymerase chain reaction</subject><subject>Potassium</subject><subject>Reductases</subject><subject>Ribonucleic acid</subject><subject>RNA</subject><subject>rRNA 16S</subject><subject>Soil bacteria</subject><subject>Soil microorganisms</subject><subject>Soil Science & Conservation</subject><subject>Water hyacinths</subject><issn>0718-9508</issn><issn>0718-9516</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2021</creationdate><recordtype>article</recordtype><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNp9kEFPGzEQhS1EJVDIH-BkiWsWxt511j6mKyBIEa3aIo6Wd-INjjZ2ajsS-RP85i4Jglvn8kaa995IHyGXDK4ZQH2TKl5zVQCHAqBUqqhOyDnUTBZKsOnp5w7yjIxTWsMwEkBAfU7ebrvOYk40dLSxfb_rQ7_PDul3g9lGZ2jw9NHlGFbWF3fu1fnV521C2fQ3jb8eZxPqXTen99ZbOmt3fmk82gk1fkmbF7txaHr6M4atjdnZw7NnM1TQ-d6g8_mFNmGzDSlfkG-d6ZMdf-iIPN3d_mnmxeLH_UMzWxRYSpGLGphpa1Ya1lYcmUEpWy4r1lWcCaUkcCFKi1i2ILhAWCJXiIy3teok8mk5IlfH3m0Mf3c2Zb0Ou-iHl5qrkgvJ-KAjwo8ujCGlaDu9jW5j4l4z0O_o9RG9HtDrA3pdDaHyGEqD2a9s_Kr-T-of_JeFbQ</recordid><startdate>20210301</startdate><enddate>20210301</enddate><creator>Chungopast, Sirinapa</creator><creator>Yodying, Preecha</creator><creator>Nomura, Mika</creator><general>Springer International Publishing</general><general>Springer Nature B.V</general><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7XB</scope><scope>88I</scope><scope>8FE</scope><scope>8FH</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>ATCPS</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>LK8</scope><scope>M2P</scope><scope>M7P</scope><scope>PATMY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PYCSY</scope><scope>Q9U</scope><orcidid>https://orcid.org/0000-0002-8856-3275</orcidid></search><sort><creationdate>20210301</creationdate><title>Effects of Cellulolytic Bacteria on Nitrogen-Fixing Bacteria, 16S rRNA, nifH Gene Abundance, and Chemical Properties of Water Hyacinth Compost</title><author>Chungopast, Sirinapa ; Yodying, Preecha ; Nomura, Mika</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c385t-701ab713a1b42c1ac88b2841f421599802553ecc3b0525c0dc29cc12b79f8c263</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2021</creationdate><topic>Abundance</topic><topic>Agriculture</topic><topic>Ammonium</topic><topic>Aquatic plants</topic><topic>Bacteria</topic><topic>Biomedical and Life Sciences</topic><topic>Cellulase</topic><topic>Cellulolytic bacteria</topic><topic>Cellulose</topic><topic>Chemical properties</topic><topic>Composting</topic><topic>Composts</topic><topic>Decomposition</topic><topic>Deoxyribonucleic acid</topic><topic>DNA</topic><topic>Ecology</topic><topic>Environment</topic><topic>Floating plants</topic><topic>Gene amplification</topic><topic>Glucose</topic><topic>Inoculum</topic><topic>Leaf litter</topic><topic>Life Sciences</topic><topic>Microbiological analysis</topic><topic>Microorganisms</topic><topic>Mineralization</topic><topic>Molecular biology</topic><topic>Mosses</topic><topic>NifH gene</topic><topic>Nitrogen</topic><topic>Nitrogen fixation</topic><topic>Nitrogen-fixing bacteria</topic><topic>Nitrogenase</topic><topic>Nitrogenase reductase</topic><topic>Nitrogenation</topic><topic>Nutrients</topic><topic>Organic matter</topic><topic>Original Paper</topic><topic>Plant Sciences</topic><topic>Polymerase chain reaction</topic><topic>Potassium</topic><topic>Reductases</topic><topic>Ribonucleic acid</topic><topic>RNA</topic><topic>rRNA 16S</topic><topic>Soil bacteria</topic><topic>Soil microorganisms</topic><topic>Soil Science & Conservation</topic><topic>Water hyacinths</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Chungopast, Sirinapa</creatorcontrib><creatorcontrib>Yodying, Preecha</creatorcontrib><creatorcontrib>Nomura, Mika</creatorcontrib><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Science Database (Alumni Edition)</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>Agricultural & Environmental Science Collection</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>ProQuest Central Student</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Biological Science Collection</collection><collection>Science Database</collection><collection>Biological Science Database</collection><collection>Environmental Science Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>Environmental Science Collection</collection><collection>ProQuest Central Basic</collection><jtitle>Journal of soil science and plant nutrition</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Chungopast, Sirinapa</au><au>Yodying, Preecha</au><au>Nomura, Mika</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Effects of Cellulolytic Bacteria on Nitrogen-Fixing Bacteria, 16S rRNA, nifH Gene Abundance, and Chemical Properties of Water Hyacinth Compost</atitle><jtitle>Journal of soil science and plant nutrition</jtitle><stitle>J Soil Sci Plant Nutr</stitle><date>2021-03-01</date><risdate>2021</risdate><volume>21</volume><issue>1</issue><spage>768</spage><epage>779</epage><pages>768-779</pages><issn>0718-9508</issn><eissn>0718-9516</eissn><abstract>This research investigated alterations to and the interdependency of nitrogen-fixing bacteria, 16S ribosomal ribonucleic acid gene (16S rRNA) and nitrogenase reductase gene (
nifH
) gene abundance, and chemical properties of water hyacinth compost when using cellulolytic bacteria isolated from soil and leaf litter (CSL) inoculum. The un- and inoculated treatments in the compost were designed with three replications. Microbiological analysis involved examination of the total number of bacteria and gene abundance in the compost based on quantitative real-time polymerase chain reaction (qPCR). Some chemical properties of the compost were also analyzed. The results indicated that applying cellulolytic bacteria into compost could increase the amounts of bacteria, especially nitrogen-fixing bacteria. The pH of the compost increased slightly for the first 4 weeks. The amount of nitrogen and organic matter (OM) in the compost increased continuously during the composting period. The concentration of ammonium changed markedly in the range 1.5–2 times at the 4th and 10th weeks of the composting process, which was consistent with an increase of nitrogen-fixing bacteria. The concentration of nitrate doubled at the 12th week. The abundance of 16S rRNA and
nifH
genes was significantly correlated with the number of bacteria, total nitrogen, ammonium, nitrate, and OM. The inoculated cellulolytic bacteria not only accelerated the nitrogen mineralization process but also promoted bacterial numbers in the compost. These bacteria also affected the transformation of nutrients and correlated positively with gene abundance.</abstract><cop>Cham</cop><pub>Springer International Publishing</pub><doi>10.1007/s42729-020-00399-4</doi><tpages>12</tpages><orcidid>https://orcid.org/0000-0002-8856-3275</orcidid></addata></record> |
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| subjects | Abundance Agriculture Ammonium Aquatic plants Bacteria Biomedical and Life Sciences Cellulase Cellulolytic bacteria Cellulose Chemical properties Composting Composts Decomposition Deoxyribonucleic acid DNA Ecology Environment Floating plants Gene amplification Glucose Inoculum Leaf litter Life Sciences Microbiological analysis Microorganisms Mineralization Molecular biology Mosses NifH gene Nitrogen Nitrogen fixation Nitrogen-fixing bacteria Nitrogenase Nitrogenase reductase Nitrogenation Nutrients Organic matter Original Paper Plant Sciences Polymerase chain reaction Potassium Reductases Ribonucleic acid RNA rRNA 16S Soil bacteria Soil microorganisms Soil Science & Conservation Water hyacinths |
| title | Effects of Cellulolytic Bacteria on Nitrogen-Fixing Bacteria, 16S rRNA, nifH Gene Abundance, and Chemical Properties of Water Hyacinth Compost |
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