Oral microbial community analysis of the patients in the progression of liver cancer

Liver disease has been reported to associate with oral microbiota. This study aimed to identify the salivary microbial structure in liver disease patients and determine whether the disease progression influence the bacterial composition. 16S rDNA high-throughput sequencing and bioinformatic analysis were used to examine oral bacterial diversity in the different status of hepatitis patients including 6 patients with Hepatitis B (Y), 6 patients with Hepatitis B Cirrhosis (YY) and 6 patients with liver cancer (C), and 6 healthy controls (T). Phylogenetic analysis revealed that the genera of Streptococcus, Prevotella, Actinomyces, Veillonella and Neisseria are predominant genus in the saliva of Y, YY, C patients and T group. Lautropia, Abiotrophia and Veillonella were enriched in Y patients, while Treponema, Selenomonas and Oribacterium were also existed in YY patients. Haemophilus, Porphyromonas and Filifactor had high abundance in C patients. The genera of Moryella, Leptotrichia, Lactobacillus, Dialister, Serratia, Enterococcus and Actinobacillus were decreased in all patient samples compared with healthy control samples which may be used for treatment of liver disease. Diversity analyses showed decreased diversity of salivary bacterial communities was discovered in the progress of the liver disease. These findings identified the oral microbiota dysbiosis in liver disease, which may providing available information and possible diagnostic biomarkers for liver patients. •The level of salivary bacterial diversity in the development of liver diseases was decreased comparing with the controls.•Those genera of Streptococcus, Porphyromonas and Actinomyces might involving in the liver cancer progress.•The genera of Lautropia, Abiotrophia and Veillonella might be used for the diagnosis of Hepatitis B patients.•Those genera of Treponema, Selenomonas and Oribacterium can be used for the diagnosis of Hepatitis B Cirrhosis patients.•Moryella, Leptotrichia, Lactobacillus, Dialister, Serratia, Enterococcus, and Actinobacillus may treat liver disease•Haemophilus, Porphyromonas and Filifactor can be used as biomarker for the diagnosis of Liver cancer