Abnormal expression of autophagy‐related proteins in immune thrombocytopenia
Autophagy is a highly conserved protein degradation pathway that is essential for affecting some autoimmune diseases. Immune thrombocytopenia (ITP) is a common autoimmune disorder, and the complex dysregulation of cellular immunity has been observed; however, the relationship between autophagy‐relat...
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| Veröffentlicht in: | Scandinavian journal of immunology 2021-02, Vol.93 (2), p.e12992-n/a, Article 12992 |
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| creator | Liu, Shu‐yan Zhang, Xiao‐mei Sun, Rui‐Jie Zhu, Jing‐jing Yuan, Dai Shan, Ning‐ning |
| description | Autophagy is a highly conserved protein degradation pathway that is essential for affecting some autoimmune diseases. Immune thrombocytopenia (ITP) is a common autoimmune disorder, and the complex dysregulation of cellular immunity has been observed; however, the relationship between autophagy‐related proteins and immune responses in ITP remains unclear. Using real‐time quantitative polymerase chain reaction (RT‐PCR), the mRNA expression levels of Beclin‐1, SQSTM1/p62 and LC3 were measured in the peripheral blood mononuclear cells (PBMCs) of 20 newly diagnosed patients with active ITP, 16 ITP patients in remission and 21 healthy volunteers. The stained Beclin‐1 and SQSTM1/p62 proteins were also observed in the bone marrow of active ITP patients and normal controls by immunofluorescence. SQSTM1/p62 mRNA expression in PBMCs in newly diagnosed patients was significantly decreased. At the same time, Beclin‐1 mRNA was increased significantly. During the remission stages, the levels of these autophagy‐related proteins were comparable with those observed in healthy controls. Taken together, these results suggest that the aberrant expression of autophagy‐related proteins might be involved in the pathogenesis of ITP. Further study of the autophagy pathway may provide a new strategy and direction for the treatment of ITP. |
| doi_str_mv | 10.1111/sji.12992 |
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Immune thrombocytopenia (ITP) is a common autoimmune disorder, and the complex dysregulation of cellular immunity has been observed; however, the relationship between autophagy‐related proteins and immune responses in ITP remains unclear. Using real‐time quantitative polymerase chain reaction (RT‐PCR), the mRNA expression levels of Beclin‐1, SQSTM1/p62 and LC3 were measured in the peripheral blood mononuclear cells (PBMCs) of 20 newly diagnosed patients with active ITP, 16 ITP patients in remission and 21 healthy volunteers. The stained Beclin‐1 and SQSTM1/p62 proteins were also observed in the bone marrow of active ITP patients and normal controls by immunofluorescence. SQSTM1/p62 mRNA expression in PBMCs in newly diagnosed patients was significantly decreased. At the same time, Beclin‐1 mRNA was increased significantly. During the remission stages, the levels of these autophagy‐related proteins were comparable with those observed in healthy controls. Taken together, these results suggest that the aberrant expression of autophagy‐related proteins might be involved in the pathogenesis of ITP. Further study of the autophagy pathway may provide a new strategy and direction for the treatment of ITP.</description><identifier>ISSN: 0300-9475</identifier><identifier>EISSN: 1365-3083</identifier><identifier>DOI: 10.1111/sji.12992</identifier><identifier>PMID: 33140452</identifier><language>eng</language><publisher>HOBOKEN: Wiley</publisher><subject>Adolescent ; Adult ; Aged ; Autoimmune diseases ; Autoimmunity - genetics ; Autophagy ; Autophagy - genetics ; Autophagy-Related Proteins - genetics ; Beclin-1 - genetics ; Beclin‐1 ; Bone marrow ; Cell-mediated immunity ; Female ; Gene expression ; Humans ; Idiopathic thrombocytopenic purpura ; Immune response ; immune thrombocytopenia ; Immunofluorescence ; Immunology ; Leukocytes (mononuclear) ; Leukocytes, Mononuclear - physiology ; Life Sciences & Biomedicine ; Male ; Middle Aged ; Peripheral blood mononuclear cells ; Phagocytosis ; Polymerase chain reaction ; Proteins ; Purpura, Thrombocytopenic, Idiopathic - genetics ; Remission ; RNA, Messenger - genetics ; RNA-Binding Proteins - genetics ; Science & Technology ; Sequestosome-1 Protein - genetics ; Thrombocytopenia ; Thrombocytopenia - genetics ; Young Adult</subject><ispartof>Scandinavian journal of immunology, 2021-02, Vol.93 (2), p.e12992-n/a, Article 12992</ispartof><rights>2020 The Scandinavian Foundation for Immunology</rights><rights>2020 The Scandinavian Foundation for Immunology.</rights><rights>Copyright © 2021 The Scandinavian Foundation for Immunology</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>true</woscitedreferencessubscribed><woscitedreferencescount>2</woscitedreferencescount><woscitedreferencesoriginalsourcerecordid>wos000590636500001</woscitedreferencesoriginalsourcerecordid><citedby>FETCH-LOGICAL-c3882-9800b1092ca27d0ba6730c60140204b39053656f57e011ba53a694ac6a1bc7363</citedby><cites>FETCH-LOGICAL-c3882-9800b1092ca27d0ba6730c60140204b39053656f57e011ba53a694ac6a1bc7363</cites><orcidid>0000-0001-6521-231X</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1111%2Fsji.12992$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1111%2Fsji.12992$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>315,781,785,1418,1434,27929,27930,39263,45579,45580,46414,46838</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/33140452$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Liu, Shu‐yan</creatorcontrib><creatorcontrib>Zhang, Xiao‐mei</creatorcontrib><creatorcontrib>Sun, Rui‐Jie</creatorcontrib><creatorcontrib>Zhu, Jing‐jing</creatorcontrib><creatorcontrib>Yuan, Dai</creatorcontrib><creatorcontrib>Shan, Ning‐ning</creatorcontrib><title>Abnormal expression of autophagy‐related proteins in immune thrombocytopenia</title><title>Scandinavian journal of immunology</title><addtitle>SCAND J IMMUNOL</addtitle><addtitle>Scand J Immunol</addtitle><description>Autophagy is a highly conserved protein degradation pathway that is essential for affecting some autoimmune diseases. Immune thrombocytopenia (ITP) is a common autoimmune disorder, and the complex dysregulation of cellular immunity has been observed; however, the relationship between autophagy‐related proteins and immune responses in ITP remains unclear. Using real‐time quantitative polymerase chain reaction (RT‐PCR), the mRNA expression levels of Beclin‐1, SQSTM1/p62 and LC3 were measured in the peripheral blood mononuclear cells (PBMCs) of 20 newly diagnosed patients with active ITP, 16 ITP patients in remission and 21 healthy volunteers. The stained Beclin‐1 and SQSTM1/p62 proteins were also observed in the bone marrow of active ITP patients and normal controls by immunofluorescence. SQSTM1/p62 mRNA expression in PBMCs in newly diagnosed patients was significantly decreased. At the same time, Beclin‐1 mRNA was increased significantly. During the remission stages, the levels of these autophagy‐related proteins were comparable with those observed in healthy controls. Taken together, these results suggest that the aberrant expression of autophagy‐related proteins might be involved in the pathogenesis of ITP. Further study of the autophagy pathway may provide a new strategy and direction for the treatment of ITP.</description><subject>Adolescent</subject><subject>Adult</subject><subject>Aged</subject><subject>Autoimmune diseases</subject><subject>Autoimmunity - genetics</subject><subject>Autophagy</subject><subject>Autophagy - genetics</subject><subject>Autophagy-Related Proteins - genetics</subject><subject>Beclin-1 - genetics</subject><subject>Beclin‐1</subject><subject>Bone marrow</subject><subject>Cell-mediated immunity</subject><subject>Female</subject><subject>Gene expression</subject><subject>Humans</subject><subject>Idiopathic thrombocytopenic purpura</subject><subject>Immune response</subject><subject>immune thrombocytopenia</subject><subject>Immunofluorescence</subject><subject>Immunology</subject><subject>Leukocytes (mononuclear)</subject><subject>Leukocytes, Mononuclear - physiology</subject><subject>Life Sciences & Biomedicine</subject><subject>Male</subject><subject>Middle Aged</subject><subject>Peripheral blood mononuclear cells</subject><subject>Phagocytosis</subject><subject>Polymerase chain reaction</subject><subject>Proteins</subject><subject>Purpura, Thrombocytopenic, Idiopathic - genetics</subject><subject>Remission</subject><subject>RNA, Messenger - genetics</subject><subject>RNA-Binding Proteins - genetics</subject><subject>Science & Technology</subject><subject>Sequestosome-1 Protein - genetics</subject><subject>Thrombocytopenia</subject><subject>Thrombocytopenia - genetics</subject><subject>Young Adult</subject><issn>0300-9475</issn><issn>1365-3083</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2021</creationdate><recordtype>article</recordtype><sourceid>HGBXW</sourceid><sourceid>EIF</sourceid><recordid>eNqN0M9u1DAQBnALgehSOPACKBIXEEo7tmM7OVYr_hRVcADOke2dUK8Se7ETlb3xCDwjT8LALj0gIeGLffiN9c3H2GMOZ5zOedmGMy66TtxhKy61qiW08i5bgQSou8aoE_aglC0Al8LI--xESt5Ao8SKvbtwMeXJjhV-3WUsJaRYpaGyy5x21_bz_se37xlHO-Om2uU0Y4ilCrEK07RErObrnCaX_J40xmAfsnuDHQs-Ot6n7NOrlx_Xb-qr968v1xdXtZdtK-quBXAcOuGtMBtwVhsJXgOlEtA42YGiNfSgDALnzippdddYry133kgtT9mzw7-U6cuCZe6nUDyOo42YltKLRhlhTGsM0ad_0W1acqR0pFrgSlMrpJ4flM-plIxDv8thsnnfc-h_ldxTyf3vksk-Of64uAk3t_JPqwTaA7hBl4biA0aPtwwAVAeaFqQX8HWY7Uytr9MSZxp98f-jpM-POoy4_3fk_sPby0P2nwq4pmE</recordid><startdate>202102</startdate><enddate>202102</enddate><creator>Liu, Shu‐yan</creator><creator>Zhang, Xiao‐mei</creator><creator>Sun, Rui‐Jie</creator><creator>Zhu, Jing‐jing</creator><creator>Yuan, Dai</creator><creator>Shan, Ning‐ning</creator><general>Wiley</general><general>Wiley Subscription Services, Inc</general><scope>BLEPL</scope><scope>DTL</scope><scope>HGBXW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QP</scope><scope>7QR</scope><scope>7T5</scope><scope>7TK</scope><scope>7U9</scope><scope>8FD</scope><scope>FR3</scope><scope>H94</scope><scope>K9.</scope><scope>M7N</scope><scope>NAPCQ</scope><scope>P64</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0001-6521-231X</orcidid></search><sort><creationdate>202102</creationdate><title>Abnormal expression of autophagy‐related proteins in immune thrombocytopenia</title><author>Liu, Shu‐yan ; Zhang, Xiao‐mei ; Sun, Rui‐Jie ; Zhu, Jing‐jing ; Yuan, Dai ; Shan, Ning‐ning</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c3882-9800b1092ca27d0ba6730c60140204b39053656f57e011ba53a694ac6a1bc7363</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2021</creationdate><topic>Adolescent</topic><topic>Adult</topic><topic>Aged</topic><topic>Autoimmune diseases</topic><topic>Autoimmunity - genetics</topic><topic>Autophagy</topic><topic>Autophagy - genetics</topic><topic>Autophagy-Related Proteins - genetics</topic><topic>Beclin-1 - genetics</topic><topic>Beclin‐1</topic><topic>Bone marrow</topic><topic>Cell-mediated immunity</topic><topic>Female</topic><topic>Gene expression</topic><topic>Humans</topic><topic>Idiopathic thrombocytopenic purpura</topic><topic>Immune response</topic><topic>immune thrombocytopenia</topic><topic>Immunofluorescence</topic><topic>Immunology</topic><topic>Leukocytes (mononuclear)</topic><topic>Leukocytes, Mononuclear - physiology</topic><topic>Life Sciences & Biomedicine</topic><topic>Male</topic><topic>Middle Aged</topic><topic>Peripheral blood mononuclear cells</topic><topic>Phagocytosis</topic><topic>Polymerase chain reaction</topic><topic>Proteins</topic><topic>Purpura, Thrombocytopenic, Idiopathic - genetics</topic><topic>Remission</topic><topic>RNA, Messenger - genetics</topic><topic>RNA-Binding Proteins - genetics</topic><topic>Science & Technology</topic><topic>Sequestosome-1 Protein - genetics</topic><topic>Thrombocytopenia</topic><topic>Thrombocytopenia - genetics</topic><topic>Young Adult</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Liu, Shu‐yan</creatorcontrib><creatorcontrib>Zhang, Xiao‐mei</creatorcontrib><creatorcontrib>Sun, Rui‐Jie</creatorcontrib><creatorcontrib>Zhu, Jing‐jing</creatorcontrib><creatorcontrib>Yuan, Dai</creatorcontrib><creatorcontrib>Shan, Ning‐ning</creatorcontrib><collection>Web of Science Core Collection</collection><collection>Science Citation Index Expanded</collection><collection>Web of Science - Science Citation Index Expanded - 2021</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Calcium & Calcified Tissue Abstracts</collection><collection>Chemoreception Abstracts</collection><collection>Immunology Abstracts</collection><collection>Neurosciences Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Nursing & Allied Health Premium</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Scandinavian journal of immunology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Liu, Shu‐yan</au><au>Zhang, Xiao‐mei</au><au>Sun, Rui‐Jie</au><au>Zhu, Jing‐jing</au><au>Yuan, Dai</au><au>Shan, Ning‐ning</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Abnormal expression of autophagy‐related proteins in immune thrombocytopenia</atitle><jtitle>Scandinavian journal of immunology</jtitle><stitle>SCAND J IMMUNOL</stitle><addtitle>Scand J Immunol</addtitle><date>2021-02</date><risdate>2021</risdate><volume>93</volume><issue>2</issue><spage>e12992</spage><epage>n/a</epage><pages>e12992-n/a</pages><artnum>12992</artnum><issn>0300-9475</issn><eissn>1365-3083</eissn><abstract>Autophagy is a highly conserved protein degradation pathway that is essential for affecting some autoimmune diseases. Immune thrombocytopenia (ITP) is a common autoimmune disorder, and the complex dysregulation of cellular immunity has been observed; however, the relationship between autophagy‐related proteins and immune responses in ITP remains unclear. Using real‐time quantitative polymerase chain reaction (RT‐PCR), the mRNA expression levels of Beclin‐1, SQSTM1/p62 and LC3 were measured in the peripheral blood mononuclear cells (PBMCs) of 20 newly diagnosed patients with active ITP, 16 ITP patients in remission and 21 healthy volunteers. The stained Beclin‐1 and SQSTM1/p62 proteins were also observed in the bone marrow of active ITP patients and normal controls by immunofluorescence. SQSTM1/p62 mRNA expression in PBMCs in newly diagnosed patients was significantly decreased. At the same time, Beclin‐1 mRNA was increased significantly. During the remission stages, the levels of these autophagy‐related proteins were comparable with those observed in healthy controls. Taken together, these results suggest that the aberrant expression of autophagy‐related proteins might be involved in the pathogenesis of ITP. Further study of the autophagy pathway may provide a new strategy and direction for the treatment of ITP.</abstract><cop>HOBOKEN</cop><pub>Wiley</pub><pmid>33140452</pmid><doi>10.1111/sji.12992</doi><tpages>8</tpages><orcidid>https://orcid.org/0000-0001-6521-231X</orcidid><oa>free_for_read</oa></addata></record> |
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| subjects | Adolescent Adult Aged Autoimmune diseases Autoimmunity - genetics Autophagy Autophagy - genetics Autophagy-Related Proteins - genetics Beclin-1 - genetics Beclin‐1 Bone marrow Cell-mediated immunity Female Gene expression Humans Idiopathic thrombocytopenic purpura Immune response immune thrombocytopenia Immunofluorescence Immunology Leukocytes (mononuclear) Leukocytes, Mononuclear - physiology Life Sciences & Biomedicine Male Middle Aged Peripheral blood mononuclear cells Phagocytosis Polymerase chain reaction Proteins Purpura, Thrombocytopenic, Idiopathic - genetics Remission RNA, Messenger - genetics RNA-Binding Proteins - genetics Science & Technology Sequestosome-1 Protein - genetics Thrombocytopenia Thrombocytopenia - genetics Young Adult |
| title | Abnormal expression of autophagy‐related proteins in immune thrombocytopenia |
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