Detection of an alcohol-associated cancer marker by single-molecule quantum sequencing

Alcoholic beverages are a well-known risk factor for cancer. N 2 -Ethyl-2′-deoxyguanosine ( N 2 -Et-dG) is a promising biomarker for alcohol-associated cancers. However, the lack of a convenient detection method for N 2 -Et-dG hinders the development of practical DNA damage markers. Herein, we develop a detection method for N 2 -Et-dG using a single-molecule quantum sequencing (SMQS) method and machine learning analysis. Our method succeeded in discriminating between N 2 -Et-dG and dG with an accuracy of 99%, using 20 signals. Our developed method quantified the mixing ratio of N 2 -Et-dG from a mixed solution of N 2 -Et-dG and dG. It is shown that our method has the potential to facilitate the development of DNA damage markers, and thus the early detection and prevention of cancers. N 2 -Ethyl-2′-deoxyguanosine, a promising biomarker of ethanol-derived cancers, was detected using a single-molecule quantum sequencer and discriminated from deoxyguanosine.