Identification, protein antiglycation, antioxidant, antiproliferative, and molecular docking of novel bioactive peptides produced from hydrolysis ofLens culinaris

Bioactive peptides produced from natural sources are considered as strategic target for drug discovery. Hyperglycemia caused protein glycation alters the structure of many tissues that impairs their functions and lead complications diseases in human body. This study investigated the bioactive peptid...

Ausführliche Beschreibung

Gespeichert in:
Bibliographische Detailangaben
Veröffentlicht in:Journal of food biochemistry 2020-12, Vol.44 (12), Article 13494
Hauptverfasser: Kuerban, Abudukadeer, Al-Malki, Abdulrahman L., Kumosani, Taha A., Sheikh, Ryan A., Al-Abbasi, Fahad Ahmed M., Alshubaily, Fawzia A., Omar Abulnaja, Khalid, Salama Moselhy, Said
Format: Artikel
Sprache:eng
Schlagworte:
Online-Zugang:Volltext
Tags: Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
container_end_page
container_issue 12
container_start_page
container_title Journal of food biochemistry
container_volume 44
creator Kuerban, Abudukadeer
Al-Malki, Abdulrahman L.
Kumosani, Taha A.
Sheikh, Ryan A.
Al-Abbasi, Fahad Ahmed M.
Alshubaily, Fawzia A.
Omar Abulnaja, Khalid
Salama Moselhy, Said
description Bioactive peptides produced from natural sources are considered as strategic target for drug discovery. Hyperglycemia caused protein glycation alters the structure of many tissues that impairs their functions and lead complications diseases in human body. This study investigated the bioactive peptides produced from red and brownLens culinaristhat might inhibit protein glycation to prevent diabetic complications. In this study, red and brownLens culinarisprotein hydrolysates were prepared by tryptic digestion, using an enzyme/substrate ratio of 1:20 (g/g), at 37 degrees C, 12 hr then peptide fractions
doi_str_mv 10.1111/jfbc.13494
format Article
fullrecord <record><control><sourceid>webofscience</sourceid><recordid>TN_cdi_webofscience_primary_000574816400001CitationCount</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>000574816400001</sourcerecordid><originalsourceid>FETCH-webofscience_primary_0005748164000013</originalsourceid><addsrcrecordid>eNqVT8tOwzAQtBCIlsKFL_AdWmzspOk5AoHEkXvl2OuyxbEjOynkd_hSHB532MvuzM7Oagi55GzFc93sbaNXXMiNPCJzvpbFUhZcHpM543muqkrOyFlKe8bY7aaUp2QmBONFJco5-Xg04Hu0qFWPwV_TLoYe0FOV2Z0bf-kJhnc0uX-DrHNoIeb9ASbK0DY40INTkZqgX9HvaLDUhwM42mBQelLSDroeDaTpkRk0GGpjaOnLaLLhmDDloyfwiWYn9CpiOicnVrkEFz99Qa7u757rh-UbNMEmjeA1bLuIrYrjNocs1rLipcwT42JBqr-ra-y_8tZh8L3436NPdEd9uQ</addsrcrecordid><sourcetype>Index Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype></control><display><type>article</type><title>Identification, protein antiglycation, antioxidant, antiproliferative, and molecular docking of novel bioactive peptides produced from hydrolysis ofLens culinaris</title><source>EBSCOhost Business Source Complete</source><source>Access via Wiley Online Library</source><source>Web of Science - Science Citation Index Expanded - 2020&lt;img src="https://exlibris-pub.s3.amazonaws.com/fromwos-v2.jpg" /&gt;</source><creator>Kuerban, Abudukadeer ; Al-Malki, Abdulrahman L. ; Kumosani, Taha A. ; Sheikh, Ryan A. ; Al-Abbasi, Fahad Ahmed M. ; Alshubaily, Fawzia A. ; Omar Abulnaja, Khalid ; Salama Moselhy, Said</creator><creatorcontrib>Kuerban, Abudukadeer ; Al-Malki, Abdulrahman L. ; Kumosani, Taha A. ; Sheikh, Ryan A. ; Al-Abbasi, Fahad Ahmed M. ; Alshubaily, Fawzia A. ; Omar Abulnaja, Khalid ; Salama Moselhy, Said</creatorcontrib><description>Bioactive peptides produced from natural sources are considered as strategic target for drug discovery. Hyperglycemia caused protein glycation alters the structure of many tissues that impairs their functions and lead complications diseases in human body. This study investigated the bioactive peptides produced from red and brownLens culinaristhat might inhibit protein glycation to prevent diabetic complications. In this study, red and brownLens culinarisprotein hydrolysates were prepared by tryptic digestion, using an enzyme/substrate ratio of 1:20 (g/g), at 37 degrees C, 12 hr then peptide fractions &lt;3 kDa were filtered by using ultrafiltration membranes. Protective ability against protein glycation, DPPH radical scavenging, and anti-proliferative activities (on HepG2, MCF-7, and PC3 cell lines) of peptide fractions were assayed in vitro. Results showed that glycation was inhibited by peptides from 28.1% to 68.3% in different test model. PC3 cell line was more sensitive to the peptides which showed strong anticancer activity with lower IC50 (0.96 mg/ml). Peptide fractions were sequenced by HPLC-MS-MS. Twenty eight novel peptides sequences was identified. In silico study, two peptides could be developed as a potential bioactive peptides exhibited antiglycation, antioxidant, and antiproliferative activities. Practical applications Peptides are becoming an emerging source of medications with the development of new technologies. We have selectedLens Culinarisas one of the rich sources of proteins to explore novel bioactive peptides encapsulated in its seeds. Peptides fractions demonstrated protective ability against protein glycation, strong antioxidant potential, and promising antiproliferative activity. We have identified 28 novel peptides and molecular docking study revealed that some peptides showed strong binding potential to insulin receptor and ACE. Thus, these peptides might be used to manage diabetes complication as well as COVID-19 disease due to their interaction with ACE. However, those peptides needs to be further studied as a potential new drug.</description><identifier>ISSN: 0145-8884</identifier><identifier>EISSN: 1745-4514</identifier><identifier>DOI: 10.1111/jfbc.13494</identifier><identifier>PMID: 33015836</identifier><language>eng</language><publisher>HOBOKEN: Wiley</publisher><subject>Biochemistry &amp; Molecular Biology ; Food Science &amp; Technology ; Life Sciences &amp; Biomedicine ; Science &amp; Technology</subject><ispartof>Journal of food biochemistry, 2020-12, Vol.44 (12), Article 13494</ispartof><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>true</woscitedreferencessubscribed><woscitedreferencescount>17</woscitedreferencescount><woscitedreferencesoriginalsourcerecordid>wos000574816400001</woscitedreferencesoriginalsourcerecordid><cites>FETCH-webofscience_primary_0005748164000013</cites><orcidid>0000-0001-5609-4913 ; 0000-0001-6875-7411 ; 0000-0002-2384-3700</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>315,782,786,27931,27932,28255</link.rule.ids></links><search><creatorcontrib>Kuerban, Abudukadeer</creatorcontrib><creatorcontrib>Al-Malki, Abdulrahman L.</creatorcontrib><creatorcontrib>Kumosani, Taha A.</creatorcontrib><creatorcontrib>Sheikh, Ryan A.</creatorcontrib><creatorcontrib>Al-Abbasi, Fahad Ahmed M.</creatorcontrib><creatorcontrib>Alshubaily, Fawzia A.</creatorcontrib><creatorcontrib>Omar Abulnaja, Khalid</creatorcontrib><creatorcontrib>Salama Moselhy, Said</creatorcontrib><title>Identification, protein antiglycation, antioxidant, antiproliferative, and molecular docking of novel bioactive peptides produced from hydrolysis ofLens culinaris</title><title>Journal of food biochemistry</title><addtitle>J FOOD BIOCHEM</addtitle><description>Bioactive peptides produced from natural sources are considered as strategic target for drug discovery. Hyperglycemia caused protein glycation alters the structure of many tissues that impairs their functions and lead complications diseases in human body. This study investigated the bioactive peptides produced from red and brownLens culinaristhat might inhibit protein glycation to prevent diabetic complications. In this study, red and brownLens culinarisprotein hydrolysates were prepared by tryptic digestion, using an enzyme/substrate ratio of 1:20 (g/g), at 37 degrees C, 12 hr then peptide fractions &lt;3 kDa were filtered by using ultrafiltration membranes. Protective ability against protein glycation, DPPH radical scavenging, and anti-proliferative activities (on HepG2, MCF-7, and PC3 cell lines) of peptide fractions were assayed in vitro. Results showed that glycation was inhibited by peptides from 28.1% to 68.3% in different test model. PC3 cell line was more sensitive to the peptides which showed strong anticancer activity with lower IC50 (0.96 mg/ml). Peptide fractions were sequenced by HPLC-MS-MS. Twenty eight novel peptides sequences was identified. In silico study, two peptides could be developed as a potential bioactive peptides exhibited antiglycation, antioxidant, and antiproliferative activities. Practical applications Peptides are becoming an emerging source of medications with the development of new technologies. We have selectedLens Culinarisas one of the rich sources of proteins to explore novel bioactive peptides encapsulated in its seeds. Peptides fractions demonstrated protective ability against protein glycation, strong antioxidant potential, and promising antiproliferative activity. We have identified 28 novel peptides and molecular docking study revealed that some peptides showed strong binding potential to insulin receptor and ACE. Thus, these peptides might be used to manage diabetes complication as well as COVID-19 disease due to their interaction with ACE. However, those peptides needs to be further studied as a potential new drug.</description><subject>Biochemistry &amp; Molecular Biology</subject><subject>Food Science &amp; Technology</subject><subject>Life Sciences &amp; Biomedicine</subject><subject>Science &amp; Technology</subject><issn>0145-8884</issn><issn>1745-4514</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2020</creationdate><recordtype>article</recordtype><sourceid>AOWDO</sourceid><recordid>eNqVT8tOwzAQtBCIlsKFL_AdWmzspOk5AoHEkXvl2OuyxbEjOynkd_hSHB532MvuzM7Oagi55GzFc93sbaNXXMiNPCJzvpbFUhZcHpM543muqkrOyFlKe8bY7aaUp2QmBONFJco5-Xg04Hu0qFWPwV_TLoYe0FOV2Z0bf-kJhnc0uX-DrHNoIeb9ASbK0DY40INTkZqgX9HvaLDUhwM42mBQelLSDroeDaTpkRk0GGpjaOnLaLLhmDDloyfwiWYn9CpiOicnVrkEFz99Qa7u757rh-UbNMEmjeA1bLuIrYrjNocs1rLipcwT42JBqr-ra-y_8tZh8L3436NPdEd9uQ</recordid><startdate>20201201</startdate><enddate>20201201</enddate><creator>Kuerban, Abudukadeer</creator><creator>Al-Malki, Abdulrahman L.</creator><creator>Kumosani, Taha A.</creator><creator>Sheikh, Ryan A.</creator><creator>Al-Abbasi, Fahad Ahmed M.</creator><creator>Alshubaily, Fawzia A.</creator><creator>Omar Abulnaja, Khalid</creator><creator>Salama Moselhy, Said</creator><general>Wiley</general><scope>AOWDO</scope><scope>BLEPL</scope><scope>DTL</scope><orcidid>https://orcid.org/0000-0001-5609-4913</orcidid><orcidid>https://orcid.org/0000-0001-6875-7411</orcidid><orcidid>https://orcid.org/0000-0002-2384-3700</orcidid></search><sort><creationdate>20201201</creationdate><title>Identification, protein antiglycation, antioxidant, antiproliferative, and molecular docking of novel bioactive peptides produced from hydrolysis ofLens culinaris</title><author>Kuerban, Abudukadeer ; Al-Malki, Abdulrahman L. ; Kumosani, Taha A. ; Sheikh, Ryan A. ; Al-Abbasi, Fahad Ahmed M. ; Alshubaily, Fawzia A. ; Omar Abulnaja, Khalid ; Salama Moselhy, Said</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-webofscience_primary_0005748164000013</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2020</creationdate><topic>Biochemistry &amp; Molecular Biology</topic><topic>Food Science &amp; Technology</topic><topic>Life Sciences &amp; Biomedicine</topic><topic>Science &amp; Technology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Kuerban, Abudukadeer</creatorcontrib><creatorcontrib>Al-Malki, Abdulrahman L.</creatorcontrib><creatorcontrib>Kumosani, Taha A.</creatorcontrib><creatorcontrib>Sheikh, Ryan A.</creatorcontrib><creatorcontrib>Al-Abbasi, Fahad Ahmed M.</creatorcontrib><creatorcontrib>Alshubaily, Fawzia A.</creatorcontrib><creatorcontrib>Omar Abulnaja, Khalid</creatorcontrib><creatorcontrib>Salama Moselhy, Said</creatorcontrib><collection>Web of Science - Science Citation Index Expanded - 2020</collection><collection>Web of Science Core Collection</collection><collection>Science Citation Index Expanded</collection><jtitle>Journal of food biochemistry</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Kuerban, Abudukadeer</au><au>Al-Malki, Abdulrahman L.</au><au>Kumosani, Taha A.</au><au>Sheikh, Ryan A.</au><au>Al-Abbasi, Fahad Ahmed M.</au><au>Alshubaily, Fawzia A.</au><au>Omar Abulnaja, Khalid</au><au>Salama Moselhy, Said</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Identification, protein antiglycation, antioxidant, antiproliferative, and molecular docking of novel bioactive peptides produced from hydrolysis ofLens culinaris</atitle><jtitle>Journal of food biochemistry</jtitle><stitle>J FOOD BIOCHEM</stitle><date>2020-12-01</date><risdate>2020</risdate><volume>44</volume><issue>12</issue><artnum>13494</artnum><issn>0145-8884</issn><eissn>1745-4514</eissn><abstract>Bioactive peptides produced from natural sources are considered as strategic target for drug discovery. Hyperglycemia caused protein glycation alters the structure of many tissues that impairs their functions and lead complications diseases in human body. This study investigated the bioactive peptides produced from red and brownLens culinaristhat might inhibit protein glycation to prevent diabetic complications. In this study, red and brownLens culinarisprotein hydrolysates were prepared by tryptic digestion, using an enzyme/substrate ratio of 1:20 (g/g), at 37 degrees C, 12 hr then peptide fractions &lt;3 kDa were filtered by using ultrafiltration membranes. Protective ability against protein glycation, DPPH radical scavenging, and anti-proliferative activities (on HepG2, MCF-7, and PC3 cell lines) of peptide fractions were assayed in vitro. Results showed that glycation was inhibited by peptides from 28.1% to 68.3% in different test model. PC3 cell line was more sensitive to the peptides which showed strong anticancer activity with lower IC50 (0.96 mg/ml). Peptide fractions were sequenced by HPLC-MS-MS. Twenty eight novel peptides sequences was identified. In silico study, two peptides could be developed as a potential bioactive peptides exhibited antiglycation, antioxidant, and antiproliferative activities. Practical applications Peptides are becoming an emerging source of medications with the development of new technologies. We have selectedLens Culinarisas one of the rich sources of proteins to explore novel bioactive peptides encapsulated in its seeds. Peptides fractions demonstrated protective ability against protein glycation, strong antioxidant potential, and promising antiproliferative activity. We have identified 28 novel peptides and molecular docking study revealed that some peptides showed strong binding potential to insulin receptor and ACE. Thus, these peptides might be used to manage diabetes complication as well as COVID-19 disease due to their interaction with ACE. However, those peptides needs to be further studied as a potential new drug.</abstract><cop>HOBOKEN</cop><pub>Wiley</pub><pmid>33015836</pmid><doi>10.1111/jfbc.13494</doi><tpages>9</tpages><orcidid>https://orcid.org/0000-0001-5609-4913</orcidid><orcidid>https://orcid.org/0000-0001-6875-7411</orcidid><orcidid>https://orcid.org/0000-0002-2384-3700</orcidid><oa>free_for_read</oa></addata></record>
fulltext fulltext
identifier ISSN: 0145-8884
ispartof Journal of food biochemistry, 2020-12, Vol.44 (12), Article 13494
issn 0145-8884
1745-4514
language eng
recordid cdi_webofscience_primary_000574816400001CitationCount
source EBSCOhost Business Source Complete; Access via Wiley Online Library; Web of Science - Science Citation Index Expanded - 2020<img src="https://exlibris-pub.s3.amazonaws.com/fromwos-v2.jpg" />
subjects Biochemistry & Molecular Biology
Food Science & Technology
Life Sciences & Biomedicine
Science & Technology
title Identification, protein antiglycation, antioxidant, antiproliferative, and molecular docking of novel bioactive peptides produced from hydrolysis ofLens culinaris
url https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-05T07%3A49%3A23IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-webofscience&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Identification,%20protein%20antiglycation,%20antioxidant,%20antiproliferative,%20and%20molecular%20docking%20of%20novel%20bioactive%20peptides%20produced%20from%20hydrolysis%20ofLens%20culinaris&rft.jtitle=Journal%20of%20food%20biochemistry&rft.au=Kuerban,%20Abudukadeer&rft.date=2020-12-01&rft.volume=44&rft.issue=12&rft.artnum=13494&rft.issn=0145-8884&rft.eissn=1745-4514&rft_id=info:doi/10.1111/jfbc.13494&rft_dat=%3Cwebofscience%3E000574816400001%3C/webofscience%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_id=info:pmid/33015836&rfr_iscdi=true