Development and characterization of microsatellite markers inStryphnodendron adstringens(Leguminosae)

In this study, we report the development and characterization of 15 new microsatellite markers forStryphnodendron adstringens(Leguminosae) in order to support future analyses of genetic diversity in populations of this species. In screening with 48 individuals from three different populations ofS. adstringens, we tested the amplification of 20 microsatellite loci, of which five are not useful for population genetic studies due to the lack of polymorphisms or amplification failures. For the final set of 15 loci, the number of alleles ranged from 2 to 15, with a total of 116 alleles. The expected heterozygosity ranged from 0.1219 to 0.8965, with an average of 0.6694 per locus. The combined probability of genetic identity (PI = 8.12 x 10(-15)) and paternity exclusion (Q = 0.99999) estimations showed that the loci may be useful to discriminate between individuals ofS. adstringens.Initial cross-amplification tests were satisfactory in three species of the genusStryphnodendron:S. rotundifolium,S. coriaceumandS. polyphyllum. This new set of markers will be a useful tool for population genetic studies, contributing to the knowledge about the evolutionary history ofS. adstringensand, additionally, correlated species.