Oxidative stress response mechanism of Scenedesmus obliquus to ionic liquids with different number of methyl-substituents

[Display omitted] •ILs affect the antioxidant enzyme activity and antioxidant content of algal cells.•IBR analysis verified the different effects of methyl-substituents on IL toxicity.•ILs affect the type, quantity, and distribution of proteins in S. obliquus.•GO annotation showed that ILs affect the oxidative stress-related proteins.•Oxidative stress response is one of the main biomarker of IL stress. Ionic liquids (ILs) have become persistent contaminants in water because of their good solubility and low biodegradability. The oxidative stress responses of Scenedesmus obliquus to three imidazole ILs with different number of methyl-substituents, i.e., 1-decyl-imidazolium chloride ([C10IM]Cl), 1-decyl-3-methylimidazolium chloride ([C10MIM]Cl), and 1-decyl-2,3-dimethylimidazolium chloride ([C10DMIM]Cl), were studied. There was a positive correlation between ROS level and IL concentration. The activities of antioxidant enzymes, i.e., superoxide dismutase, catalase, peroxidase, ascorbate peroxidase, and glutathione peroxidase, and the content of antioxidants, i.e., ascorbic acid and glutathione, changed in IL treatment with a concentration-dependent effect. Proline accumulation increased with increasing IL concentration. Integrated biomarker response (IBR) index analysis, based on the eight oxidative stress response indicators, revealed that the toxicity order was: [C10IM]Cl < [C10DMIM]Cl < [C10MIM]Cl. Proteomic analysis showed that IL affect the type and distribution of proteins in S. obliquus. Chloroplast and photosystem II were affected as cellular component, and the proteins related to oxidative stress are annotated in GO categories. IBR index and proteomic analysis indicate that oxidative stress response is one of the main biomarkers of IL stress.