miR-4634 augments the anti-tumor effects of RAD001 and associates well with clinical prognosis of non-small cell lung cancer

MicroRNA (miRNA) is involved in the physiological and pathological processes of various malignancies. In this study, miRNA microarray analysis showed that miR-4634 levels in A549 cells increased significantly after everolimus (RAD001) treatment. Decreased expression of miR-4634 was also found in non...

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Veröffentlicht in:	Scientific reports 2020-08, Vol.10 (1), p.13079, Article 13079
Hauptverfasser:	Liu, Sile, Zang, Hongjing, Zheng, Hongmei, Wang, Weiyuan, Wen, Qiuyuan, Zhan, Yuting, Yang, Yang, Ning, Yue, Wang, Haihua, Fan, Songqing
Format:	Artikel
Sprache:	eng
Schlagworte:	631/154 631/67 A549 Cells Adenocarcinoma Antineoplastic Agents - pharmacology Antineoplastic Agents - therapeutic use Antitumor activity Carcinogenesis - drug effects Carcinogenesis - genetics Carcinoma, Non-Small-Cell Lung - diagnosis Carcinoma, Non-Small-Cell Lung - drug therapy Carcinoma, Non-Small-Cell Lung - genetics Carcinoma, Non-Small-Cell Lung - pathology Cell migration Cell Movement - drug effects Cell Movement - genetics Cell proliferation Everolimus - pharmacology Everolimus - therapeutic use Female Gene Expression Regulation, Neoplastic - drug effects Humanities and Social Sciences Humans Hybridization Lung cancer Lung carcinoma Lung Neoplasms - diagnosis Lung Neoplasms - drug therapy Lung Neoplasms - genetics Lung Neoplasms - pathology Lymph nodes Male Metastases MicroRNAs MicroRNAs - genetics Middle Aged miRNA multidisciplinary Multivariate analysis Non-small cell lung carcinoma Patients Prognosis Science Science (multidisciplinary) Small cell lung carcinoma Squamous cell carcinoma TOR protein Tumor cell lines Tumor suppressor genes Tumors
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Zusammenfassung:	MicroRNA (miRNA) is involved in the physiological and pathological processes of various malignancies. In this study, miRNA microarray analysis showed that miR-4634 levels in A549 cells increased significantly after everolimus (RAD001) treatment. Decreased expression of miR-4634 was also found in non-small-cell lung carcinoma (NSCLC) cell lines and patients’ tumors by qPCR. Additionally, a combination of miR-4634 and RAD001 exerted synergistic antitumor efficacy by inhibiting cell proliferation, migration, and colony formation. High expression of miR-4634 was significantly more common in non-cancerous lung tissue than adenocarcinoma or squamous cell carcinoma tissue (72.8%, 45.7%, and 50.9%, respectively; P
ISSN:	2045-2322 2045-2322
DOI:	10.1038/s41598-020-70157-0