Quo vadis FRET? Förster's method in the era of superresolution

Although the theoretical foundations of Förster resonance energy transfer (FRET) were laid in the 1940s as part of the quantum physical revolution of the 20th century, it was only in the 1970s that it made its way to biology as a result of the availability of suitable measuring and labeling technolo...

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Veröffentlicht in:Methods and applications in fluorescence 2020-07, Vol.8 (3), p.32003-032003, Article 032003
Hauptverfasser: Szabó, Ágnes, Szendi-Szatmári, Tímea, Szöll si, János, Nagy, Peter
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container_title Methods and applications in fluorescence
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creator Szabó, Ágnes
Szendi-Szatmári, Tímea
Szöll si, János
Nagy, Peter
description Although the theoretical foundations of Förster resonance energy transfer (FRET) were laid in the 1940s as part of the quantum physical revolution of the 20th century, it was only in the 1970s that it made its way to biology as a result of the availability of suitable measuring and labeling technologies. Thanks to its ease of application, FRET became widely used for studying molecular associations on the nanometer scale. The development of superresolution techniques at the turn of the millennium promised an unprecedented insight into the structure and function of molecular complexes. Without downplaying the significance of superresolution microscopies this review expresses our view that FRET is still a legitimate tool in the armamentarium of biologists for studying molecular associations since it offers distinct advantages and overcomes certain limitations of superresolution approaches.
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source IOP Publishing Journals; Web of Science - Science Citation Index Expanded - 2020<img src="https://exlibris-pub.s3.amazonaws.com/fromwos-v2.jpg" />; Institute of Physics (IOP) Journals - HEAL-Link
subjects Chemistry
Chemistry, Analytical
Chemistry, Physical
FRET
Förster resonance energy transfer
Physical Sciences
Science & Technology
superresolution microscopy
title Quo vadis FRET? Förster's method in the era of superresolution
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