Quo vadis FRET? Förster's method in the era of superresolution
Although the theoretical foundations of Förster resonance energy transfer (FRET) were laid in the 1940s as part of the quantum physical revolution of the 20th century, it was only in the 1970s that it made its way to biology as a result of the availability of suitable measuring and labeling technolo...
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Veröffentlicht in: | Methods and applications in fluorescence 2020-07, Vol.8 (3), p.32003-032003, Article 032003 |
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creator | Szabó, Ágnes Szendi-Szatmári, Tímea Szöll si, János Nagy, Peter |
description | Although the theoretical foundations of Förster resonance energy transfer (FRET) were laid in the 1940s as part of the quantum physical revolution of the 20th century, it was only in the 1970s that it made its way to biology as a result of the availability of suitable measuring and labeling technologies. Thanks to its ease of application, FRET became widely used for studying molecular associations on the nanometer scale. The development of superresolution techniques at the turn of the millennium promised an unprecedented insight into the structure and function of molecular complexes. Without downplaying the significance of superresolution microscopies this review expresses our view that FRET is still a legitimate tool in the armamentarium of biologists for studying molecular associations since it offers distinct advantages and overcomes certain limitations of superresolution approaches. |
doi_str_mv | 10.1088/2050-6120/ab9b72 |
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subjects | Chemistry Chemistry, Analytical Chemistry, Physical FRET Förster resonance energy transfer Physical Sciences Science & Technology superresolution microscopy |
title | Quo vadis FRET? Förster's method in the era of superresolution |
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