Regulation of Myofilament Contractile Function in Human Donor and Failing Hearts

Heart failure (HF) often includes changes in myocardial contractile function. This study addressed the myofibrillar basis for contractile dysfunction in failing human myocardium. Regulation of contractile properties was measured in cardiac myocyte preparations isolated from frozen, left ventricular mid-wall biopsies of donor (n= 7) and failing human hearts (n= 8). Permeabilized cardiac myocyte preparations were attached between a force transducer and a position motor, and both the Ca(2+)dependence and sarcomere length (SL) dependence of force, rate of force, loaded shortening, and power output were measured at 15 +/- 1 degrees C. The myocyte preparation size was similar between groups (donor: length 148 +/- 10 mu m, width 21 +/- 2 mu m,n= 13; HF: length 131 +/- 9 mu m, width 23 +/- 1 mu m,n= 16). The maximal Ca2+-activated isometric force was also similar between groups (donor: 47 +/- 4 kN.m(-2); HF: 44 +/- 5 kN.m(-2)), which implicates that previously reported force declines in multi-cellular preparations reflect, at least in part, tissue remodeling. Maximal force development rates were also similar between groups (donor:k(tr)= 0.60 +/- 0.05 s(-1); HF: k(tr)= 0.55 +/- 0.04 s(-1)), and both groups exhibited similar Ca(2+)activation dependence ofk(tr)values. Human cardiac myocyte preparations exhibited a Ca(2+)activation dependence of loaded shortening and power output. The peak power output normalized to isometric force (PNPO) decreased by similar to 12% from maximal Ca(2+)to half-maximal Ca(2+)activations in both groups. Interestingly, the SL dependence of PNPO was diminished in failing myocyte preparations. During sub-maximal Ca(2+)activation, a reduction in SL from similar to 2.25 to similar to 1.95 mu m caused a similar to 26% decline in PNPO in donor myocytes but only an similar to 11% change in failing myocytes. These results suggest that altered length-dependent regulation of myofilament function impairs ventricular performance in failing human hearts.