Zika structural genes determine the virulence of African and Asian lineages
The Asian lineage of Zika virus (ZIKV) is responsible for the recent epidemics in the Americas and severe disease, whereas the African lineage of ZIKV has not been reported to cause epidemics or severe disease. We constructed a cDNA infectious clone (IC) of an African ZIKV strain, which, together wi...
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| Veröffentlicht in: | Emerging microbes & infections 2020-01, Vol.9 (1), p.1023-1033 |
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| creator | Nunes, Bruno T. D. Fontes-Garfias, Camila R. Shan, Chao Muruato, Antonio E. Nunes, Jannyce G. C. Burbano, Rommel M. R. Vasconcelos, Pedro F. C. Shi, Pei-Yong Medeiros, Daniele B. A. |
| description | The Asian lineage of Zika virus (ZIKV) is responsible for the recent epidemics in the Americas and severe disease, whereas the African lineage of ZIKV has not been reported to cause epidemics or severe disease. We constructed a cDNA infectious clone (IC) of an African ZIKV strain, which, together with our previously developed Asian ZIKV strain IC, allowed us to engineer chimeric viruses by swapping the structural and non-structural genes between the two lineages. Recombinant parental and chimeric viruses were analyzed in A129 and newborn CD1 mouse models. In the A129 mice, the African strain developed higher viremia, organ viral loading, and mortality rate. In CD1 mice, the African strain exhibited a higher neurovirulence than the Asian strain. A chimeric virus containing the structural genes from the African strain is more virulent than the Asian strain, whereas a chimeric virus containing the non-structural genes from the African strain exhibited a virulence comparable to the Asian strain. These results suggest that (i) African strain is more virulent than Asian strain and (ii) viral structural genes primarily determine the virulence difference between the two lineages in mouse models. Other factors may contribute to the discrepancy between the mouse and epidemic results. |
| doi_str_mv | 10.1080/22221751.2020.1753583 |
| format | Article |
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D. ; Fontes-Garfias, Camila R. ; Shan, Chao ; Muruato, Antonio E. ; Nunes, Jannyce G. C. ; Burbano, Rommel M. R. ; Vasconcelos, Pedro F. C. ; Shi, Pei-Yong ; Medeiros, Daniele B. A.</creator><creatorcontrib>Nunes, Bruno T. D. ; Fontes-Garfias, Camila R. ; Shan, Chao ; Muruato, Antonio E. ; Nunes, Jannyce G. C. ; Burbano, Rommel M. R. ; Vasconcelos, Pedro F. C. ; Shi, Pei-Yong ; Medeiros, Daniele B. A.</creatorcontrib><description>The Asian lineage of Zika virus (ZIKV) is responsible for the recent epidemics in the Americas and severe disease, whereas the African lineage of ZIKV has not been reported to cause epidemics or severe disease. We constructed a cDNA infectious clone (IC) of an African ZIKV strain, which, together with our previously developed Asian ZIKV strain IC, allowed us to engineer chimeric viruses by swapping the structural and non-structural genes between the two lineages. Recombinant parental and chimeric viruses were analyzed in A129 and newborn CD1 mouse models. In the A129 mice, the African strain developed higher viremia, organ viral loading, and mortality rate. In CD1 mice, the African strain exhibited a higher neurovirulence than the Asian strain. A chimeric virus containing the structural genes from the African strain is more virulent than the Asian strain, whereas a chimeric virus containing the non-structural genes from the African strain exhibited a virulence comparable to the Asian strain. These results suggest that (i) African strain is more virulent than Asian strain and (ii) viral structural genes primarily determine the virulence difference between the two lineages in mouse models. Other factors may contribute to the discrepancy between the mouse and epidemic results.</description><identifier>ISSN: 2222-1751</identifier><identifier>EISSN: 2222-1751</identifier><identifier>DOI: 10.1080/22221751.2020.1753583</identifier><identifier>PMID: 32419649</identifier><language>eng</language><publisher>ABINGDON: Taylor & Francis</publisher><subject>Africa ; African ; Americas - epidemiology ; Animals ; Asia ; Asian ; Chlorocebus aethiops ; Disease Models, Animal ; Epidemics ; Genes, Viral ; Genetic Variation ; Humans ; Immunology ; Infectious Diseases ; Life Sciences & Biomedicine ; lineages ; Mice ; Microbiology ; Science & Technology ; Vero Cells ; Virulence ; Virulence - genetics ; Zika ; Zika virus ; Zika Virus - genetics ; Zika Virus - isolation & purification ; Zika Virus - pathogenicity ; Zika Virus Infection - pathology</subject><ispartof>Emerging microbes & infections, 2020-01, Vol.9 (1), p.1023-1033</ispartof><rights>2020 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group, on behalf of Shanghai Shangyixun Cultural Communication Co., Ltd 2020</rights><rights>2020 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group, on behalf of Shanghai Shangyixun Cultural Communication Co., Ltd. This work is licensed under the Creative Commons Attribution License http://creativecommons.org/licenses/by/4.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>2020 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group, on behalf of Shanghai Shangyixun Cultural Communication Co., Ltd 2020 The Author(s)</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>true</woscitedreferencessubscribed><woscitedreferencescount>11</woscitedreferencescount><woscitedreferencesoriginalsourcerecordid>wos000537275800001</woscitedreferencesoriginalsourcerecordid><citedby>FETCH-LOGICAL-c562t-da98bc47601d68345635d4e237dc79c3b3c5b696cb57f637a6f6ad4af33872f23</citedby><cites>FETCH-LOGICAL-c562t-da98bc47601d68345635d4e237dc79c3b3c5b696cb57f637a6f6ad4af33872f23</cites><orcidid>0000-0002-0793-9665 ; 0000-0002-1912-8413 ; 0000-0002-4872-234X ; 0000-0002-3953-3782</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC8284969/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC8284969/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,315,729,782,786,866,887,2106,2118,27511,27933,27934,28257,53800,53802,59152,59153</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/32419649$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Nunes, Bruno T. D.</creatorcontrib><creatorcontrib>Fontes-Garfias, Camila R.</creatorcontrib><creatorcontrib>Shan, Chao</creatorcontrib><creatorcontrib>Muruato, Antonio E.</creatorcontrib><creatorcontrib>Nunes, Jannyce G. C.</creatorcontrib><creatorcontrib>Burbano, Rommel M. R.</creatorcontrib><creatorcontrib>Vasconcelos, Pedro F. C.</creatorcontrib><creatorcontrib>Shi, Pei-Yong</creatorcontrib><creatorcontrib>Medeiros, Daniele B. A.</creatorcontrib><title>Zika structural genes determine the virulence of African and Asian lineages</title><title>Emerging microbes & infections</title><addtitle>EMERG MICROBES INFEC</addtitle><addtitle>Emerg Microbes Infect</addtitle><description>The Asian lineage of Zika virus (ZIKV) is responsible for the recent epidemics in the Americas and severe disease, whereas the African lineage of ZIKV has not been reported to cause epidemics or severe disease. 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Other factors may contribute to the discrepancy between the mouse and epidemic results.</description><subject>Africa</subject><subject>African</subject><subject>Americas - epidemiology</subject><subject>Animals</subject><subject>Asia</subject><subject>Asian</subject><subject>Chlorocebus aethiops</subject><subject>Disease Models, Animal</subject><subject>Epidemics</subject><subject>Genes, Viral</subject><subject>Genetic Variation</subject><subject>Humans</subject><subject>Immunology</subject><subject>Infectious Diseases</subject><subject>Life Sciences & Biomedicine</subject><subject>lineages</subject><subject>Mice</subject><subject>Microbiology</subject><subject>Science & Technology</subject><subject>Vero Cells</subject><subject>Virulence</subject><subject>Virulence - genetics</subject><subject>Zika</subject><subject>Zika virus</subject><subject>Zika Virus - genetics</subject><subject>Zika Virus - isolation & purification</subject><subject>Zika Virus - pathogenicity</subject><subject>Zika Virus Infection - pathology</subject><issn>2222-1751</issn><issn>2222-1751</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2020</creationdate><recordtype>article</recordtype><sourceid>0YH</sourceid><sourceid>AOWDO</sourceid><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>DOA</sourceid><recordid>eNqNkk1vEzEQhlcIRKvSnwBaiQtSleL1914QUcRHRSUucOFizfojddjYxfa26r_HS9Ko5YDwxdbMM69m5nXTvOzQeYckeovr6QTrzjHCNSQYYZI8aY7n-GJOPH3wPmpOc96gegTitKPPmyOCaddz2h83X374n9DmkiZdpgRju7bB5tbYYtPWB9uWK9ve-DSNNmjbRtcuXfIaQgvBtMvs62usHKxtftE8czBme7q_T5rvHz98W31eXH79dLFaXi4047gsDPRy0FRw1BkuCWWcMEMtJsJo0WsyEM0G3nM9MOE4EcAdB0PBESIFdpicNBc7XRNho66T30K6UxG8-hOIaa0gFa9Hq6QgepCUa8oxpQjkIAcOmmhjMdIUqta7ndb1NGyt0TaUuoVHoo8zwV-pdbxREkva874KvNkLpPhrsrmorc_ajiMEG6esMEWUiDrm3Pfrv9BNnFKoq1KYoTob76SsFNtROsWck3WHZjqkZvfVvftqdl_t3a91rx5Ocqi697oCZzvg1g7RZe1nRw9Y_R6MCCyYnH9KV2n5__TKFyg-hlWcQqml73elPriYtnAb02hUgbsxJpcgaJ8V-fcwvwEhbd4s</recordid><startdate>20200101</startdate><enddate>20200101</enddate><creator>Nunes, Bruno T. 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D.</au><au>Fontes-Garfias, Camila R.</au><au>Shan, Chao</au><au>Muruato, Antonio E.</au><au>Nunes, Jannyce G. C.</au><au>Burbano, Rommel M. R.</au><au>Vasconcelos, Pedro F. C.</au><au>Shi, Pei-Yong</au><au>Medeiros, Daniele B. A.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Zika structural genes determine the virulence of African and Asian lineages</atitle><jtitle>Emerging microbes & infections</jtitle><stitle>EMERG MICROBES INFEC</stitle><addtitle>Emerg Microbes Infect</addtitle><date>2020-01-01</date><risdate>2020</risdate><volume>9</volume><issue>1</issue><spage>1023</spage><epage>1033</epage><pages>1023-1033</pages><issn>2222-1751</issn><eissn>2222-1751</eissn><abstract>The Asian lineage of Zika virus (ZIKV) is responsible for the recent epidemics in the Americas and severe disease, whereas the African lineage of ZIKV has not been reported to cause epidemics or severe disease. We constructed a cDNA infectious clone (IC) of an African ZIKV strain, which, together with our previously developed Asian ZIKV strain IC, allowed us to engineer chimeric viruses by swapping the structural and non-structural genes between the two lineages. Recombinant parental and chimeric viruses were analyzed in A129 and newborn CD1 mouse models. In the A129 mice, the African strain developed higher viremia, organ viral loading, and mortality rate. In CD1 mice, the African strain exhibited a higher neurovirulence than the Asian strain. A chimeric virus containing the structural genes from the African strain is more virulent than the Asian strain, whereas a chimeric virus containing the non-structural genes from the African strain exhibited a virulence comparable to the Asian strain. These results suggest that (i) African strain is more virulent than Asian strain and (ii) viral structural genes primarily determine the virulence difference between the two lineages in mouse models. 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| subjects | Africa African Americas - epidemiology Animals Asia Asian Chlorocebus aethiops Disease Models, Animal Epidemics Genes, Viral Genetic Variation Humans Immunology Infectious Diseases Life Sciences & Biomedicine lineages Mice Microbiology Science & Technology Vero Cells Virulence Virulence - genetics Zika Zika virus Zika Virus - genetics Zika Virus - isolation & purification Zika Virus - pathogenicity Zika Virus Infection - pathology |
| title | Zika structural genes determine the virulence of African and Asian lineages |
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