Rudanella paleaurantiibacter sp. nov., Isolated from Activated Sludge

A Gram-stain-negative, orange-colored bacterium, designated HX-22-17 T , was isolated from activated sludge of an agricultural chemical plant in Maanshan, Anhui province, China (118° 52′ E 31° 68′ N). The strain was strictly aerobic, non-endospore forming, non-motile, and ellipse. Growth of the strain was observed at 16–42 °C (optimum between 25 and 30 °C) at pH 6.0–9.0 (optimum at pH 7.0) and with 0–6.0% (w/v) NaCl (optimum at 1.0%). 16S rRNA gene sequence analysis showed that the strain was most closely related to Rudanella lutea KACC 12603 T (99.5% similarity). The predominant cellular fatty acids were summed feature 3 (C 16:1 ω 7 c and/or C 16:1 ω 6 c ), iso-C 15:0 , and anteiso-C 15:0 . The major polar lipids included posphatidylethanolamine (PE), aminolipid (AL), and phospholipids (PL). The genomic DNA G+C content of the strain was 54.1 mol%. The ANI and dDDH values obtained between the genomes of HX-22-17 T and R. lutea KACC 12603 T were 89.3% and 39.3%, respectively. The phenotypic, chemotaxonomic, and genotypic data clearly showed that strain HX-22-17 T represents a novel species of the genus Rudanella , for which the name R. paleaurantiibacter sp. nov. is proposed. The type strain is HX-22-17 T (=KCTC 72656 T = CCTCC AB 2019347 T ).