Enrichment of Cysteine-Containing Peptide by On-Resin Capturing and Fixed Charge Tag Derivatization for Sensitive ESI-MS Detection

High complexity of cell and tissue proteomes limits the investigation of proteomic biomarkers. Therefore, the methods of enrichment of some chemical groups of peptides including thiopeptides are important tools that may facilitate the proteomic analysis by reducing sample complexity and increasing p...

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Veröffentlicht in:	Molecules (Basel, Switzerland) Switzerland), 2020-03, Vol.25 (6), p.1372, Article 1372
Hauptverfasser:	Bachor, Remigiusz, Gorzen, Oliwia, Rola, Anna, Mojsa, Karolina, Panek-Laszczynska, Karolina, Konieczny, Andrzej, Dabrowska, Krystyna, Witkiewicz, Wojciech, Szewczuk, Zbigniew
Format:	Artikel
Sprache:	eng
Schlagworte:	Amino acids Animals Biochemistry & Molecular Biology Chemistry Chemistry, Multidisciplinary Chromatography Conjugation Cysteine Cysteine - chemistry Efficiency Enrichment Experiments fixed charge tags Ionization Ions Life Sciences & Biomedicine Lysine Lysine - chemistry maleimide Mass spectrometry Mass spectroscopy Mice Peptides Peptides - chemistry Physical Sciences podocin Proteins Proteomes Resins Science & Technology Scientific imaging Tandem Mass Spectrometry thiopeptides Tryptic peptides
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