Low prevalence of antifungal resistant Candida africana, in the C. albicans complex causing vulvovaginal candidiasis
The Candida (C.) albicans complex includes C. albicans, C. dubliniensis, C. stellatoidea, and C. africana, with the last mentioned as an important emerging agent of vulvovaginal candidiasis (VVC). The aim of the study was to identify C. africana and C. dubliniensis and assess their drug susceptibili...
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| Veröffentlicht in: | Heliyon 2020-03, Vol.6 (3), p.e03619-e03619, Article e03619 |
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| creator | Farahyar, Shirin Izadi, Samira Razmjou, Elham Falahati, Mehraban Roudbary, Maryam Ashrafi-Khozani, Mahtab Ansari, Saham Fattahi, Azam Ghahri-Mobaser, Zahra Rahimi, Maryam |
| description | The Candida (C.) albicans complex includes C. albicans, C. dubliniensis, C. stellatoidea, and C. africana, with the last mentioned as an important emerging agent of vulvovaginal candidiasis (VVC). The aim of the study was to identify C. africana and C. dubliniensis and assess their drug susceptibility in vaginitis. One-hundred Candida isolates of the C. albicans complex from women diagnosed with vaginitis and from vaginal samples in the culture collection of a medical mycology laboratory were examined. Species of the C. albicans complex were identified with conventional and molecular methods using polymerase chain reaction (PCR) for amplification and sequencing of the internal transcribed spacer (ITS) region, PCR for partial amplification of hyphal wall protein 1 (HWP1) gene and duplex PCR. The effects of antifungal drugs were evaluated according to standard broth microdilution protocols.
Ninety-seven C. albicans (97%) and three C. africana (3%) isolates were identified. Results of susceptibility testing revealed one isolate of C. africana to be resistant to both clotrimazole and fluconazole, and one showed reduced susceptibility to itraconazole.
Identification of Candida species especially C. africana in vaginitis is crucial, there are varying levels of resistance to antifungal drugs.
Microbiology; Mycology; Microbial genomics; DNA sequencing; Antimicrobial; Women's health; Candida albicans complex; Candida dubliniensis; Candida africana; Drug susceptibility; Vulvovaginal candidiasis; HWP1. |
| doi_str_mv | 10.1016/j.heliyon.2020.e03619 |
| format | Article |
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Ninety-seven C. albicans (97%) and three C. africana (3%) isolates were identified. Results of susceptibility testing revealed one isolate of C. africana to be resistant to both clotrimazole and fluconazole, and one showed reduced susceptibility to itraconazole.
Identification of Candida species especially C. africana in vaginitis is crucial, there are varying levels of resistance to antifungal drugs.
Microbiology; Mycology; Microbial genomics; DNA sequencing; Antimicrobial; Women's health; Candida albicans complex; Candida dubliniensis; Candida africana; Drug susceptibility; Vulvovaginal candidiasis; HWP1.</description><identifier>ISSN: 2405-8440</identifier><identifier>EISSN: 2405-8440</identifier><identifier>DOI: 10.1016/j.heliyon.2020.e03619</identifier><identifier>PMID: 32215332</identifier><language>eng</language><publisher>England: Elsevier Ltd</publisher><subject>Antimicrobial ; Candida ; Candida africana ; Candida albicans complex ; Candida dubliniensis ; clotrimazole ; DNA sequencing ; Drug susceptibility ; fluconazole ; genes ; HWP1 ; hyphae ; internal transcribed spacers ; itraconazole ; Microbial genomics ; Microbiology ; minimum inhibitory concentration ; Mycology ; polymerase chain reaction ; species ; vaginitis ; Vulvovaginal candidiasis ; Women's health</subject><ispartof>Heliyon, 2020-03, Vol.6 (3), p.e03619-e03619, Article e03619</ispartof><rights>2020</rights><rights>2020 Published by Elsevier Ltd.</rights><rights>2020 Published by Elsevier Ltd. 2020</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c566t-92c2797ffe22efa6f32f3fa9123775fedb3286e29032fb01e07554ee9238335c3</citedby><cites>FETCH-LOGICAL-c566t-92c2797ffe22efa6f32f3fa9123775fedb3286e29032fb01e07554ee9238335c3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC7090347/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC7090347/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,724,777,781,861,882,2096,27905,27906,53772,53774</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/32215332$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Farahyar, Shirin</creatorcontrib><creatorcontrib>Izadi, Samira</creatorcontrib><creatorcontrib>Razmjou, Elham</creatorcontrib><creatorcontrib>Falahati, Mehraban</creatorcontrib><creatorcontrib>Roudbary, Maryam</creatorcontrib><creatorcontrib>Ashrafi-Khozani, Mahtab</creatorcontrib><creatorcontrib>Ansari, Saham</creatorcontrib><creatorcontrib>Fattahi, Azam</creatorcontrib><creatorcontrib>Ghahri-Mobaser, Zahra</creatorcontrib><creatorcontrib>Rahimi, Maryam</creatorcontrib><title>Low prevalence of antifungal resistant Candida africana, in the C. albicans complex causing vulvovaginal candidiasis</title><title>Heliyon</title><addtitle>Heliyon</addtitle><description>The Candida (C.) albicans complex includes C. albicans, C. dubliniensis, C. stellatoidea, and C. africana, with the last mentioned as an important emerging agent of vulvovaginal candidiasis (VVC). The aim of the study was to identify C. africana and C. dubliniensis and assess their drug susceptibility in vaginitis. One-hundred Candida isolates of the C. albicans complex from women diagnosed with vaginitis and from vaginal samples in the culture collection of a medical mycology laboratory were examined. Species of the C. albicans complex were identified with conventional and molecular methods using polymerase chain reaction (PCR) for amplification and sequencing of the internal transcribed spacer (ITS) region, PCR for partial amplification of hyphal wall protein 1 (HWP1) gene and duplex PCR. The effects of antifungal drugs were evaluated according to standard broth microdilution protocols.
Ninety-seven C. albicans (97%) and three C. africana (3%) isolates were identified. Results of susceptibility testing revealed one isolate of C. africana to be resistant to both clotrimazole and fluconazole, and one showed reduced susceptibility to itraconazole.
Identification of Candida species especially C. africana in vaginitis is crucial, there are varying levels of resistance to antifungal drugs.
Microbiology; Mycology; Microbial genomics; DNA sequencing; Antimicrobial; Women's health; Candida albicans complex; Candida dubliniensis; Candida africana; Drug susceptibility; Vulvovaginal candidiasis; HWP1.</description><subject>Antimicrobial</subject><subject>Candida</subject><subject>Candida africana</subject><subject>Candida albicans complex</subject><subject>Candida dubliniensis</subject><subject>clotrimazole</subject><subject>DNA sequencing</subject><subject>Drug susceptibility</subject><subject>fluconazole</subject><subject>genes</subject><subject>HWP1</subject><subject>hyphae</subject><subject>internal transcribed spacers</subject><subject>itraconazole</subject><subject>Microbial genomics</subject><subject>Microbiology</subject><subject>minimum inhibitory concentration</subject><subject>Mycology</subject><subject>polymerase chain reaction</subject><subject>species</subject><subject>vaginitis</subject><subject>Vulvovaginal candidiasis</subject><subject>Women's health</subject><issn>2405-8440</issn><issn>2405-8440</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2020</creationdate><recordtype>article</recordtype><sourceid>DOA</sourceid><recordid>eNqFks1uEzEQx1cIRKvSRwD5yIEEf-7HBYQiPipV4gJna9Y7Thw5drB3t_RteBaeDKcJVXvqydbMf34zY_-r6jWjS0ZZ_X673KB3tzEsOeV0iVTUrHtWnXNJ1aKVkj5_cD-rLnPeUkqZauuuES-rM8E5U0Lw82q6jjdkn3AGj8EgiZZAGJ2dwho8SZhdHkuArCAMbgACNjkDAd4RF8i4QbJa_v0Dvj8EMzFxt_f4mxiYsgtrMk9-jjOsXSgwc4dwUJCvqhcWfMbL03lR_fzy-cfq2-L6-9er1afrhVF1PS46bnjTNdYi52ihtoJbYaFjXDSNsjj0grc18o6WRE8Z0kYpidhx0QqhjLioro7cIcJW75PbQbrVEZy-C8S01pBGZzzqtgHGmGS0N0J2VAIo0yhKrTFDXyMrrA9H1n7qdzgYDGMC_wj6OBPcRq_jrBta5pNNAbw9AVL8NWEe9c5lg95DwDhlzRWXXJXe9dNS0UrOJKe0SNVRalLMOaG9n4hRfTCL3uqTWfTBLPpollL35uE691X_rVEEH48CLB80O0w6G3fwyOASmrG8oHuixT985NVn</recordid><startdate>20200301</startdate><enddate>20200301</enddate><creator>Farahyar, Shirin</creator><creator>Izadi, Samira</creator><creator>Razmjou, Elham</creator><creator>Falahati, Mehraban</creator><creator>Roudbary, Maryam</creator><creator>Ashrafi-Khozani, Mahtab</creator><creator>Ansari, Saham</creator><creator>Fattahi, Azam</creator><creator>Ghahri-Mobaser, Zahra</creator><creator>Rahimi, Maryam</creator><general>Elsevier Ltd</general><general>Elsevier</general><scope>6I.</scope><scope>AAFTH</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>7S9</scope><scope>L.6</scope><scope>5PM</scope><scope>DOA</scope></search><sort><creationdate>20200301</creationdate><title>Low prevalence of antifungal resistant Candida africana, in the C. albicans complex causing vulvovaginal candidiasis</title><author>Farahyar, Shirin ; Izadi, Samira ; Razmjou, Elham ; Falahati, Mehraban ; Roudbary, Maryam ; Ashrafi-Khozani, Mahtab ; Ansari, Saham ; Fattahi, Azam ; Ghahri-Mobaser, Zahra ; Rahimi, Maryam</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c566t-92c2797ffe22efa6f32f3fa9123775fedb3286e29032fb01e07554ee9238335c3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2020</creationdate><topic>Antimicrobial</topic><topic>Candida</topic><topic>Candida africana</topic><topic>Candida albicans complex</topic><topic>Candida dubliniensis</topic><topic>clotrimazole</topic><topic>DNA sequencing</topic><topic>Drug susceptibility</topic><topic>fluconazole</topic><topic>genes</topic><topic>HWP1</topic><topic>hyphae</topic><topic>internal transcribed spacers</topic><topic>itraconazole</topic><topic>Microbial genomics</topic><topic>Microbiology</topic><topic>minimum inhibitory concentration</topic><topic>Mycology</topic><topic>polymerase chain reaction</topic><topic>species</topic><topic>vaginitis</topic><topic>Vulvovaginal candidiasis</topic><topic>Women's health</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Farahyar, Shirin</creatorcontrib><creatorcontrib>Izadi, Samira</creatorcontrib><creatorcontrib>Razmjou, Elham</creatorcontrib><creatorcontrib>Falahati, Mehraban</creatorcontrib><creatorcontrib>Roudbary, Maryam</creatorcontrib><creatorcontrib>Ashrafi-Khozani, Mahtab</creatorcontrib><creatorcontrib>Ansari, Saham</creatorcontrib><creatorcontrib>Fattahi, Azam</creatorcontrib><creatorcontrib>Ghahri-Mobaser, Zahra</creatorcontrib><creatorcontrib>Rahimi, Maryam</creatorcontrib><collection>ScienceDirect Open Access Titles</collection><collection>Elsevier:ScienceDirect:Open Access</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>AGRICOLA</collection><collection>AGRICOLA - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><collection>DOAJ Directory of Open Access Journals</collection><jtitle>Heliyon</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Farahyar, Shirin</au><au>Izadi, Samira</au><au>Razmjou, Elham</au><au>Falahati, Mehraban</au><au>Roudbary, Maryam</au><au>Ashrafi-Khozani, Mahtab</au><au>Ansari, Saham</au><au>Fattahi, Azam</au><au>Ghahri-Mobaser, Zahra</au><au>Rahimi, Maryam</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Low prevalence of antifungal resistant Candida africana, in the C. albicans complex causing vulvovaginal candidiasis</atitle><jtitle>Heliyon</jtitle><addtitle>Heliyon</addtitle><date>2020-03-01</date><risdate>2020</risdate><volume>6</volume><issue>3</issue><spage>e03619</spage><epage>e03619</epage><pages>e03619-e03619</pages><artnum>e03619</artnum><issn>2405-8440</issn><eissn>2405-8440</eissn><abstract>The Candida (C.) albicans complex includes C. albicans, C. dubliniensis, C. stellatoidea, and C. africana, with the last mentioned as an important emerging agent of vulvovaginal candidiasis (VVC). The aim of the study was to identify C. africana and C. dubliniensis and assess their drug susceptibility in vaginitis. One-hundred Candida isolates of the C. albicans complex from women diagnosed with vaginitis and from vaginal samples in the culture collection of a medical mycology laboratory were examined. Species of the C. albicans complex were identified with conventional and molecular methods using polymerase chain reaction (PCR) for amplification and sequencing of the internal transcribed spacer (ITS) region, PCR for partial amplification of hyphal wall protein 1 (HWP1) gene and duplex PCR. The effects of antifungal drugs were evaluated according to standard broth microdilution protocols.
Ninety-seven C. albicans (97%) and three C. africana (3%) isolates were identified. Results of susceptibility testing revealed one isolate of C. africana to be resistant to both clotrimazole and fluconazole, and one showed reduced susceptibility to itraconazole.
Identification of Candida species especially C. africana in vaginitis is crucial, there are varying levels of resistance to antifungal drugs.
Microbiology; Mycology; Microbial genomics; DNA sequencing; Antimicrobial; Women's health; Candida albicans complex; Candida dubliniensis; Candida africana; Drug susceptibility; Vulvovaginal candidiasis; HWP1.</abstract><cop>England</cop><pub>Elsevier Ltd</pub><pmid>32215332</pmid><doi>10.1016/j.heliyon.2020.e03619</doi><oa>free_for_read</oa></addata></record> |
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| subjects | Antimicrobial Candida Candida africana Candida albicans complex Candida dubliniensis clotrimazole DNA sequencing Drug susceptibility fluconazole genes HWP1 hyphae internal transcribed spacers itraconazole Microbial genomics Microbiology minimum inhibitory concentration Mycology polymerase chain reaction species vaginitis Vulvovaginal candidiasis Women's health |
| title | Low prevalence of antifungal resistant Candida africana, in the C. albicans complex causing vulvovaginal candidiasis |
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