Parental Transfer of Microcystin-LR-Induced Innate Immune Dysfunction of Zebrafish: A Cross-Generational Study
Transgenerational effects of microcystin-LR (MC-LR) released by cyanobacterial blooms have become a hot topic. In the present study, adult zebrafish pairs were exposed to 0, 0.4, 2, and 10 μg/L MC-LR for 60 days and the embryos (F1 generation) were hatched without or with continued MC-LR exposures a...
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| Veröffentlicht in: | Environmental science & technology 2020-01, Vol.54 (2), p.1014-1023 |
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| creator | Lin, Wang Guo, Honghui Wang, Lingkai Zhang, Dandan Wu, Xueyang Li, Li Qiu, Yuming Yang, Liping Li, Dapeng Tang, Rong |
| description | Transgenerational effects of microcystin-LR (MC-LR) released by cyanobacterial blooms have become a hot topic. In the present study, adult zebrafish pairs were exposed to 0, 0.4, 2, and 10 μg/L MC-LR for 60 days and the embryos (F1 generation) were hatched without or with continued MC-LR exposures at the same concentrations until 5 days postfertilization (dpf). The results showed the existence of MC-LR both in F0 gonads and in F1 embryos and indicated that MC-LR could be transferred directly from the F0 adult fish to F1 offspring. The adverse effects on sex hormone levels, sexual development, and fecundity in F0 generation along with abnormal development in F1 offspring were observed. Furthermore, downregulation of antioxidant genes (cat, mn-sod, gpx1a) and upregulation of innate immune-related genes (tlr4a, myd88, tnfα, il1β) as well as increased proinflammation cytokine contents (TNF-α, IL-1β, IL-6) were noticed in F1 offspring without/with continued MC-LR exposures. In addition, significant differences between the two F1 embryo treatments demonstrated that continuous MC-LR exposure could result in a higher degree of inflammatory response compared to those without MC-LR exposure. Our findings revealed that MC-LR could exert cross-generational effects of immunotoxicity by inhibiting the antioxidant system and activating an inflammatory response. |
| doi_str_mv | 10.1021/acs.est.9b04953 |
| format | Article |
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In the present study, adult zebrafish pairs were exposed to 0, 0.4, 2, and 10 μg/L MC-LR for 60 days and the embryos (F1 generation) were hatched without or with continued MC-LR exposures at the same concentrations until 5 days postfertilization (dpf). The results showed the existence of MC-LR both in F0 gonads and in F1 embryos and indicated that MC-LR could be transferred directly from the F0 adult fish to F1 offspring. The adverse effects on sex hormone levels, sexual development, and fecundity in F0 generation along with abnormal development in F1 offspring were observed. Furthermore, downregulation of antioxidant genes (cat, mn-sod, gpx1a) and upregulation of innate immune-related genes (tlr4a, myd88, tnfα, il1β) as well as increased proinflammation cytokine contents (TNF-α, IL-1β, IL-6) were noticed in F1 offspring without/with continued MC-LR exposures. In addition, significant differences between the two F1 embryo treatments demonstrated that continuous MC-LR exposure could result in a higher degree of inflammatory response compared to those without MC-LR exposure. Our findings revealed that MC-LR could exert cross-generational effects of immunotoxicity by inhibiting the antioxidant system and activating an inflammatory response.</description><identifier>ISSN: 0013-936X</identifier><identifier>EISSN: 1520-5851</identifier><identifier>DOI: 10.1021/acs.est.9b04953</identifier><identifier>PMID: 31859493</identifier><language>eng</language><publisher>WASHINGTON: American Chemical Society</publisher><subject>Animals ; Antioxidants ; Cytokines ; Embryos ; Engineering ; Engineering, Environmental ; Environmental Sciences ; Environmental Sciences & Ecology ; Exposure ; Fecundity ; Genes ; Genetic crosses ; Gonads ; IL-1β ; Immunity, Innate ; Immunotoxicity ; Inflammation ; Inflammatory response ; Interleukin 1 ; Interleukin 6 ; Life Sciences & Biomedicine ; Marine Toxins ; Microcystin-LR ; Microcystins ; MyD88 protein ; Offspring ; Oxidoreductases ; Science & Technology ; Sex hormones ; Technology ; Tumor necrosis factor-α ; Water Pollutants, Chemical ; Zebrafish</subject><ispartof>Environmental science & technology, 2020-01, Vol.54 (2), p.1014-1023</ispartof><rights>Copyright American Chemical Society Jan 21, 2020</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>true</woscitedreferencessubscribed><woscitedreferencescount>38</woscitedreferencescount><woscitedreferencesoriginalsourcerecordid>wos000509419700039</woscitedreferencesoriginalsourcerecordid><citedby>FETCH-LOGICAL-a361t-a7e470aa2f2881e9a41c77b2f94a0ac03f1f17f5c7051b7d24be85bd3b891863</citedby><cites>FETCH-LOGICAL-a361t-a7e470aa2f2881e9a41c77b2f94a0ac03f1f17f5c7051b7d24be85bd3b891863</cites><orcidid>0000-0002-2243-4614 ; 0000-0002-8232-3094 ; 0000-0001-5631-1463</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://pubs.acs.org/doi/pdf/10.1021/acs.est.9b04953$$EPDF$$P50$$Gacs$$H</linktopdf><linktohtml>$$Uhttps://pubs.acs.org/doi/10.1021/acs.est.9b04953$$EHTML$$P50$$Gacs$$H</linktohtml><link.rule.ids>315,781,785,2766,27080,27928,27929,56742,56792</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/31859493$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Lin, Wang</creatorcontrib><creatorcontrib>Guo, Honghui</creatorcontrib><creatorcontrib>Wang, Lingkai</creatorcontrib><creatorcontrib>Zhang, Dandan</creatorcontrib><creatorcontrib>Wu, Xueyang</creatorcontrib><creatorcontrib>Li, Li</creatorcontrib><creatorcontrib>Qiu, Yuming</creatorcontrib><creatorcontrib>Yang, Liping</creatorcontrib><creatorcontrib>Li, Dapeng</creatorcontrib><creatorcontrib>Tang, Rong</creatorcontrib><title>Parental Transfer of Microcystin-LR-Induced Innate Immune Dysfunction of Zebrafish: A Cross-Generational Study</title><title>Environmental science & technology</title><addtitle>ENVIRON SCI TECHNOL</addtitle><addtitle>Environ. Sci. Technol</addtitle><description>Transgenerational effects of microcystin-LR (MC-LR) released by cyanobacterial blooms have become a hot topic. In the present study, adult zebrafish pairs were exposed to 0, 0.4, 2, and 10 μg/L MC-LR for 60 days and the embryos (F1 generation) were hatched without or with continued MC-LR exposures at the same concentrations until 5 days postfertilization (dpf). The results showed the existence of MC-LR both in F0 gonads and in F1 embryos and indicated that MC-LR could be transferred directly from the F0 adult fish to F1 offspring. The adverse effects on sex hormone levels, sexual development, and fecundity in F0 generation along with abnormal development in F1 offspring were observed. Furthermore, downregulation of antioxidant genes (cat, mn-sod, gpx1a) and upregulation of innate immune-related genes (tlr4a, myd88, tnfα, il1β) as well as increased proinflammation cytokine contents (TNF-α, IL-1β, IL-6) were noticed in F1 offspring without/with continued MC-LR exposures. In addition, significant differences between the two F1 embryo treatments demonstrated that continuous MC-LR exposure could result in a higher degree of inflammatory response compared to those without MC-LR exposure. Our findings revealed that MC-LR could exert cross-generational effects of immunotoxicity by inhibiting the antioxidant system and activating an inflammatory response.</description><subject>Animals</subject><subject>Antioxidants</subject><subject>Cytokines</subject><subject>Embryos</subject><subject>Engineering</subject><subject>Engineering, Environmental</subject><subject>Environmental Sciences</subject><subject>Environmental Sciences & Ecology</subject><subject>Exposure</subject><subject>Fecundity</subject><subject>Genes</subject><subject>Genetic crosses</subject><subject>Gonads</subject><subject>IL-1β</subject><subject>Immunity, Innate</subject><subject>Immunotoxicity</subject><subject>Inflammation</subject><subject>Inflammatory response</subject><subject>Interleukin 1</subject><subject>Interleukin 6</subject><subject>Life Sciences & Biomedicine</subject><subject>Marine Toxins</subject><subject>Microcystin-LR</subject><subject>Microcystins</subject><subject>MyD88 protein</subject><subject>Offspring</subject><subject>Oxidoreductases</subject><subject>Science & Technology</subject><subject>Sex hormones</subject><subject>Technology</subject><subject>Tumor necrosis factor-α</subject><subject>Water Pollutants, Chemical</subject><subject>Zebrafish</subject><issn>0013-936X</issn><issn>1520-5851</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2020</creationdate><recordtype>article</recordtype><sourceid>AOWDO</sourceid><sourceid>EIF</sourceid><recordid>eNqNkU1vEzEURS0EoqGwZodGYokmff6KbXbVACVSUCvIArEZ2Z5nMVXjKfaMUP49HhLSVSVW9uLc5_uOCXlNYUmB0Qvr8xLzuDQOhJH8CVlQyaCWWtKnZAFAeW346vsZeZHzLQAwDvo5OeNUSyMMX5B4YxPG0d5V22RjDpiqIVRfep8Gv89jH-vN13odu8ljV61jtCNW691uilh92OcwRT_2Q5wzP9AlG_r88311WTVpyLm-wojJzkCZ_22cuv1L8izYu4yvjuc52X76uG0-15vrq3VzuaktX9GxtgqFAmtZYFpTNFZQr5RjwQgL1gMPNFAVpFcgqVMdEw61dB132lC94ufk7WHsfRp-TUVQeztMqbTILeNCsBWAUYW6OFB-bpswtPep39m0bym0s9626G3n9FFvSbw5zp3cDrsT_89nAd4dgN_ohpB9j9HjCSsfIMEIalS5cVNo_f90049_VTbDFMeHh-aKp-Ue6_0H8u2nAQ</recordid><startdate>20200121</startdate><enddate>20200121</enddate><creator>Lin, Wang</creator><creator>Guo, Honghui</creator><creator>Wang, Lingkai</creator><creator>Zhang, Dandan</creator><creator>Wu, Xueyang</creator><creator>Li, Li</creator><creator>Qiu, Yuming</creator><creator>Yang, Liping</creator><creator>Li, Dapeng</creator><creator>Tang, Rong</creator><general>American Chemical Society</general><general>Amer Chemical Soc</general><scope>AOWDO</scope><scope>BLEPL</scope><scope>DTL</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QO</scope><scope>7ST</scope><scope>7T7</scope><scope>7U7</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>P64</scope><scope>SOI</scope><orcidid>https://orcid.org/0000-0002-2243-4614</orcidid><orcidid>https://orcid.org/0000-0002-8232-3094</orcidid><orcidid>https://orcid.org/0000-0001-5631-1463</orcidid></search><sort><creationdate>20200121</creationdate><title>Parental Transfer of Microcystin-LR-Induced Innate Immune Dysfunction of Zebrafish: A Cross-Generational Study</title><author>Lin, Wang ; Guo, Honghui ; Wang, Lingkai ; Zhang, Dandan ; Wu, Xueyang ; Li, Li ; Qiu, Yuming ; Yang, Liping ; Li, Dapeng ; Tang, Rong</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-a361t-a7e470aa2f2881e9a41c77b2f94a0ac03f1f17f5c7051b7d24be85bd3b891863</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2020</creationdate><topic>Animals</topic><topic>Antioxidants</topic><topic>Cytokines</topic><topic>Embryos</topic><topic>Engineering</topic><topic>Engineering, Environmental</topic><topic>Environmental Sciences</topic><topic>Environmental Sciences & Ecology</topic><topic>Exposure</topic><topic>Fecundity</topic><topic>Genes</topic><topic>Genetic crosses</topic><topic>Gonads</topic><topic>IL-1β</topic><topic>Immunity, Innate</topic><topic>Immunotoxicity</topic><topic>Inflammation</topic><topic>Inflammatory response</topic><topic>Interleukin 1</topic><topic>Interleukin 6</topic><topic>Life Sciences & Biomedicine</topic><topic>Marine Toxins</topic><topic>Microcystin-LR</topic><topic>Microcystins</topic><topic>MyD88 protein</topic><topic>Offspring</topic><topic>Oxidoreductases</topic><topic>Science & Technology</topic><topic>Sex hormones</topic><topic>Technology</topic><topic>Tumor necrosis factor-α</topic><topic>Water Pollutants, Chemical</topic><topic>Zebrafish</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Lin, Wang</creatorcontrib><creatorcontrib>Guo, Honghui</creatorcontrib><creatorcontrib>Wang, Lingkai</creatorcontrib><creatorcontrib>Zhang, Dandan</creatorcontrib><creatorcontrib>Wu, Xueyang</creatorcontrib><creatorcontrib>Li, Li</creatorcontrib><creatorcontrib>Qiu, Yuming</creatorcontrib><creatorcontrib>Yang, Liping</creatorcontrib><creatorcontrib>Li, Dapeng</creatorcontrib><creatorcontrib>Tang, Rong</creatorcontrib><collection>Web of Science - Science Citation Index Expanded - 2020</collection><collection>Web of Science Core Collection</collection><collection>Science Citation Index Expanded</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Biotechnology Research Abstracts</collection><collection>Environment Abstracts</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Toxicology Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Environment Abstracts</collection><jtitle>Environmental science & technology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Lin, Wang</au><au>Guo, Honghui</au><au>Wang, Lingkai</au><au>Zhang, Dandan</au><au>Wu, Xueyang</au><au>Li, Li</au><au>Qiu, Yuming</au><au>Yang, Liping</au><au>Li, Dapeng</au><au>Tang, Rong</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Parental Transfer of Microcystin-LR-Induced Innate Immune Dysfunction of Zebrafish: A Cross-Generational Study</atitle><jtitle>Environmental science & technology</jtitle><stitle>ENVIRON SCI TECHNOL</stitle><addtitle>Environ. Sci. Technol</addtitle><date>2020-01-21</date><risdate>2020</risdate><volume>54</volume><issue>2</issue><spage>1014</spage><epage>1023</epage><pages>1014-1023</pages><issn>0013-936X</issn><eissn>1520-5851</eissn><abstract>Transgenerational effects of microcystin-LR (MC-LR) released by cyanobacterial blooms have become a hot topic. In the present study, adult zebrafish pairs were exposed to 0, 0.4, 2, and 10 μg/L MC-LR for 60 days and the embryos (F1 generation) were hatched without or with continued MC-LR exposures at the same concentrations until 5 days postfertilization (dpf). The results showed the existence of MC-LR both in F0 gonads and in F1 embryos and indicated that MC-LR could be transferred directly from the F0 adult fish to F1 offspring. The adverse effects on sex hormone levels, sexual development, and fecundity in F0 generation along with abnormal development in F1 offspring were observed. Furthermore, downregulation of antioxidant genes (cat, mn-sod, gpx1a) and upregulation of innate immune-related genes (tlr4a, myd88, tnfα, il1β) as well as increased proinflammation cytokine contents (TNF-α, IL-1β, IL-6) were noticed in F1 offspring without/with continued MC-LR exposures. In addition, significant differences between the two F1 embryo treatments demonstrated that continuous MC-LR exposure could result in a higher degree of inflammatory response compared to those without MC-LR exposure. Our findings revealed that MC-LR could exert cross-generational effects of immunotoxicity by inhibiting the antioxidant system and activating an inflammatory response.</abstract><cop>WASHINGTON</cop><pub>American Chemical Society</pub><pmid>31859493</pmid><doi>10.1021/acs.est.9b04953</doi><tpages>10</tpages><orcidid>https://orcid.org/0000-0002-2243-4614</orcidid><orcidid>https://orcid.org/0000-0002-8232-3094</orcidid><orcidid>https://orcid.org/0000-0001-5631-1463</orcidid></addata></record> |
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| subjects | Animals Antioxidants Cytokines Embryos Engineering Engineering, Environmental Environmental Sciences Environmental Sciences & Ecology Exposure Fecundity Genes Genetic crosses Gonads IL-1β Immunity, Innate Immunotoxicity Inflammation Inflammatory response Interleukin 1 Interleukin 6 Life Sciences & Biomedicine Marine Toxins Microcystin-LR Microcystins MyD88 protein Offspring Oxidoreductases Science & Technology Sex hormones Technology Tumor necrosis factor-α Water Pollutants, Chemical Zebrafish |
| title | Parental Transfer of Microcystin-LR-Induced Innate Immune Dysfunction of Zebrafish: A Cross-Generational Study |
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