Using TTchem-seq for profiling nascent transcription and measuring transcript elongation

The dynamics of transcription can be studied genome wide by high-throughput sequencing of nascent and newly synthesized RNA. 4-thiouridine (4SU) labeling in vivo enables the specific capture of such new transcripts, with 4SU residues being tagged by biotin linkers and captured using streptavidin bea...

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Veröffentlicht in:	Nature protocols 2020-02, Vol.15 (2), p.604-627
Hauptverfasser:	Gregersen, Lea H., Mitter, Richard, Svejstrup, Jesper Q.
Format:	Artikel
Sprache:	eng
Schlagworte:	631/1647/2017 631/337/572 Analytical Chemistry Beads Biochemical Research Methods Biochemistry & Molecular Biology Bioinformatics Biological Techniques Biomedical and Life Sciences Biotin Computational Biology/Bioinformatics Computer applications Data analysis DNA-directed RNA polymerase Elongation Fragmentation Gene expression Gene sequencing Genomes High resolution In vivo methods and tests Labeling Life Sciences Life Sciences & Biomedicine Mammalian cells Marking and tracking techniques Microarrays Molecular biology Next-generation sequencing Organic Chemistry Protocol RNA polymerase RNA polymerase II Science & Technology Streptavidin Transcription elongation Transcriptomes
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