Single-Cell Analysis for Glycogen Localization and Metabolism in Cultured Astrocytes
Cerebral glycogen is principally localized in astrocytes rather than in neurons. Glycogen metabolism has been implicated in higher brain functions, including learning and memory, yet the distribution patterns of glycogen in different types of astrocytes have not been fully described. Here, we applie...
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| Veröffentlicht in: | Cellular and molecular neurobiology 2020-07, Vol.40 (5), p.801-812 |
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| container_title | Cellular and molecular neurobiology |
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| creator | Zhu, Yuanyuan Fan, Ze Wang, Rui Xie, Rougang Guo, Haiyun Zhang, Ming Guo, Baolin Sun, Tangna Zhang, Haifeng Zhuo, Lixia Li, Yan Wu, Shengxi |
| description | Cerebral glycogen is principally localized in astrocytes rather than in neurons. Glycogen metabolism has been implicated in higher brain functions, including learning and memory, yet the distribution patterns of glycogen in different types of astrocytes have not been fully described. Here, we applied a method based on the incorporation of 2-NBDG, a
d
-glucose fluorescent derivative that can trace glycogen, to investigate glycogen’s distribution in the brain. We identified two types of astrocytes, namely, 2-NBDG
I
(glycogen-deficient) and 2-NBDG
II
(glycogen-rich) cells. Whole-cell patch-clamp and fluorescence-activated cell sorting (FACS) were used to separate 2-NBDG
II
astrocytes from 2-NBDG
I
astrocytes. The expression levels of glycogen metabolic enzymes were analyzed in 2-NBDG
I
and 2-NBDG
II
astrocytes. We found unique glycogen metabolic patterns between 2-NBDG
I
and 2-NBDG
II
astrocytes. We also observed that 2-NBDG
II
astrocytes were mainly identified as fibrous astrocytes but not protoplasmic astrocytes. Our data reveal cell type-dependent glycogen distribution and metabolism patterns, suggesting diverse functions of these different astrocytes. |
| doi_str_mv | 10.1007/s10571-019-00775-4 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_webof</sourceid><recordid>TN_cdi_webofscience_primary_000503714600001</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>2407863128</sourcerecordid><originalsourceid>FETCH-LOGICAL-c474t-ce8e059eac8096ccbad1fed251e7c3ee7cfa82b2c713112acc1c956aeebfa9bf3</originalsourceid><addsrcrecordid>eNqNkU9v1DAQxS0EokvhC3BAkTgiw9hO4uSCtIqgIC3iQDlbjjNZXHntYjug5dPjNmWBC-LiP_Jv3ozfI-Qpg5cMQL5KDBrJKLCelqtsaH2PbFgjBW07AffJBrjktBY1nJFHKV0BQA_QPCRngnWt4JxtyOUn6_cO6YDOVVuv3THZVM0hVhfuaMIefbULRjv7Q2cbfKX9VH3ArMfgbDpU1lfD4vIScaq2KcdgjhnTY_Jg1i7hk7v9nHx---ZyeEd3Hy_eD9sdNbWsMzXYITQ9atNB3xoz6onNOPGGoTQCyzLrjo_cSCYY49oYZvqm1YjjrPtxFufk9ap7vYwHnAz6HLVT19EedDyqoK36-8XbL2ofvinJW8a7ugg8vxOI4euCKaursMTiQlK8BllMKlih-EqZGFKKOJ86MFA3Sag1CVWSULdJqBvpZ3_Odir5ZX0BuhX4jmOYk7HoDZ6wklUDQrK6LSdgg823_g9h8bmUvvj_0kKLlU6F8HuMvz_5j_l_AnMAt6U</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2407863128</pqid></control><display><type>article</type><title>Single-Cell Analysis for Glycogen Localization and Metabolism in Cultured Astrocytes</title><source>MEDLINE</source><source>SpringerNature Journals</source><source>Web of Science - Science Citation Index Expanded - 2020<img src="https://exlibris-pub.s3.amazonaws.com/fromwos-v2.jpg" /></source><creator>Zhu, Yuanyuan ; Fan, Ze ; Wang, Rui ; Xie, Rougang ; Guo, Haiyun ; Zhang, Ming ; Guo, Baolin ; Sun, Tangna ; Zhang, Haifeng ; Zhuo, Lixia ; Li, Yan ; Wu, Shengxi</creator><creatorcontrib>Zhu, Yuanyuan ; Fan, Ze ; Wang, Rui ; Xie, Rougang ; Guo, Haiyun ; Zhang, Ming ; Guo, Baolin ; Sun, Tangna ; Zhang, Haifeng ; Zhuo, Lixia ; Li, Yan ; Wu, Shengxi</creatorcontrib><description>Cerebral glycogen is principally localized in astrocytes rather than in neurons. Glycogen metabolism has been implicated in higher brain functions, including learning and memory, yet the distribution patterns of glycogen in different types of astrocytes have not been fully described. Here, we applied a method based on the incorporation of 2-NBDG, a
d
-glucose fluorescent derivative that can trace glycogen, to investigate glycogen’s distribution in the brain. We identified two types of astrocytes, namely, 2-NBDG
I
(glycogen-deficient) and 2-NBDG
II
(glycogen-rich) cells. Whole-cell patch-clamp and fluorescence-activated cell sorting (FACS) were used to separate 2-NBDG
II
astrocytes from 2-NBDG
I
astrocytes. The expression levels of glycogen metabolic enzymes were analyzed in 2-NBDG
I
and 2-NBDG
II
astrocytes. We found unique glycogen metabolic patterns between 2-NBDG
I
and 2-NBDG
II
astrocytes. We also observed that 2-NBDG
II
astrocytes were mainly identified as fibrous astrocytes but not protoplasmic astrocytes. Our data reveal cell type-dependent glycogen distribution and metabolism patterns, suggesting diverse functions of these different astrocytes.</description><identifier>ISSN: 0272-4340</identifier><identifier>EISSN: 1573-6830</identifier><identifier>DOI: 10.1007/s10571-019-00775-4</identifier><identifier>PMID: 31863221</identifier><language>eng</language><publisher>New York: Springer US</publisher><subject>4-Chloro-7-nitrobenzofurazan - analogs & derivatives ; 4-Chloro-7-nitrobenzofurazan - chemistry ; Animals ; Astrocytes ; Astrocytes - chemistry ; Astrocytes - metabolism ; Biomedical and Life Sciences ; Biomedicine ; Cell Biology ; Cells, Cultured ; Cerebral Cortex - metabolism ; Deoxyglucose - analogs & derivatives ; Deoxyglucose - chemistry ; Flow cytometry ; Glucose ; Glycogen ; Glycogen - analysis ; Glycogen - deficiency ; Glycogen - metabolism ; Life Sciences & Biomedicine ; Localization ; Metabolism ; Mice ; Mice, Inbred C57BL ; Neurobiology ; Neurons - metabolism ; Neurosciences ; Neurosciences & Neurology ; Original Research ; Science & Technology ; Single-Cell Analysis - methods</subject><ispartof>Cellular and molecular neurobiology, 2020-07, Vol.40 (5), p.801-812</ispartof><rights>The Author(s) 2019</rights><rights>The Author(s) 2019. This work is published under https://creativecommons.org/licenses/by/4.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>true</woscitedreferencessubscribed><woscitedreferencescount>11</woscitedreferencescount><woscitedreferencesoriginalsourcerecordid>wos000503714600001</woscitedreferencesoriginalsourcerecordid><citedby>FETCH-LOGICAL-c474t-ce8e059eac8096ccbad1fed251e7c3ee7cfa82b2c713112acc1c956aeebfa9bf3</citedby><cites>FETCH-LOGICAL-c474t-ce8e059eac8096ccbad1fed251e7c3ee7cfa82b2c713112acc1c956aeebfa9bf3</cites><orcidid>0000-0002-3210-9567 ; 0000-0002-7465-7705 ; 0000-0001-7644-1492</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s10571-019-00775-4$$EPDF$$P50$$Gspringer$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s10571-019-00775-4$$EHTML$$P50$$Gspringer$$Hfree_for_read</linktohtml><link.rule.ids>230,315,782,786,887,27931,27932,28255,41495,42564,51326</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/31863221$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Zhu, Yuanyuan</creatorcontrib><creatorcontrib>Fan, Ze</creatorcontrib><creatorcontrib>Wang, Rui</creatorcontrib><creatorcontrib>Xie, Rougang</creatorcontrib><creatorcontrib>Guo, Haiyun</creatorcontrib><creatorcontrib>Zhang, Ming</creatorcontrib><creatorcontrib>Guo, Baolin</creatorcontrib><creatorcontrib>Sun, Tangna</creatorcontrib><creatorcontrib>Zhang, Haifeng</creatorcontrib><creatorcontrib>Zhuo, Lixia</creatorcontrib><creatorcontrib>Li, Yan</creatorcontrib><creatorcontrib>Wu, Shengxi</creatorcontrib><title>Single-Cell Analysis for Glycogen Localization and Metabolism in Cultured Astrocytes</title><title>Cellular and molecular neurobiology</title><addtitle>Cell Mol Neurobiol</addtitle><addtitle>CELL MOL NEUROBIOL</addtitle><addtitle>Cell Mol Neurobiol</addtitle><description>Cerebral glycogen is principally localized in astrocytes rather than in neurons. Glycogen metabolism has been implicated in higher brain functions, including learning and memory, yet the distribution patterns of glycogen in different types of astrocytes have not been fully described. Here, we applied a method based on the incorporation of 2-NBDG, a
d
-glucose fluorescent derivative that can trace glycogen, to investigate glycogen’s distribution in the brain. We identified two types of astrocytes, namely, 2-NBDG
I
(glycogen-deficient) and 2-NBDG
II
(glycogen-rich) cells. Whole-cell patch-clamp and fluorescence-activated cell sorting (FACS) were used to separate 2-NBDG
II
astrocytes from 2-NBDG
I
astrocytes. The expression levels of glycogen metabolic enzymes were analyzed in 2-NBDG
I
and 2-NBDG
II
astrocytes. We found unique glycogen metabolic patterns between 2-NBDG
I
and 2-NBDG
II
astrocytes. We also observed that 2-NBDG
II
astrocytes were mainly identified as fibrous astrocytes but not protoplasmic astrocytes. Our data reveal cell type-dependent glycogen distribution and metabolism patterns, suggesting diverse functions of these different astrocytes.</description><subject>4-Chloro-7-nitrobenzofurazan - analogs & derivatives</subject><subject>4-Chloro-7-nitrobenzofurazan - chemistry</subject><subject>Animals</subject><subject>Astrocytes</subject><subject>Astrocytes - chemistry</subject><subject>Astrocytes - metabolism</subject><subject>Biomedical and Life Sciences</subject><subject>Biomedicine</subject><subject>Cell Biology</subject><subject>Cells, Cultured</subject><subject>Cerebral Cortex - metabolism</subject><subject>Deoxyglucose - analogs & derivatives</subject><subject>Deoxyglucose - chemistry</subject><subject>Flow cytometry</subject><subject>Glucose</subject><subject>Glycogen</subject><subject>Glycogen - analysis</subject><subject>Glycogen - deficiency</subject><subject>Glycogen - metabolism</subject><subject>Life Sciences & Biomedicine</subject><subject>Localization</subject><subject>Metabolism</subject><subject>Mice</subject><subject>Mice, Inbred C57BL</subject><subject>Neurobiology</subject><subject>Neurons - metabolism</subject><subject>Neurosciences</subject><subject>Neurosciences & Neurology</subject><subject>Original Research</subject><subject>Science & Technology</subject><subject>Single-Cell Analysis - methods</subject><issn>0272-4340</issn><issn>1573-6830</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2020</creationdate><recordtype>article</recordtype><sourceid>C6C</sourceid><sourceid>AOWDO</sourceid><sourceid>EIF</sourceid><recordid>eNqNkU9v1DAQxS0EokvhC3BAkTgiw9hO4uSCtIqgIC3iQDlbjjNZXHntYjug5dPjNmWBC-LiP_Jv3ozfI-Qpg5cMQL5KDBrJKLCelqtsaH2PbFgjBW07AffJBrjktBY1nJFHKV0BQA_QPCRngnWt4JxtyOUn6_cO6YDOVVuv3THZVM0hVhfuaMIefbULRjv7Q2cbfKX9VH3ArMfgbDpU1lfD4vIScaq2KcdgjhnTY_Jg1i7hk7v9nHx---ZyeEd3Hy_eD9sdNbWsMzXYITQ9atNB3xoz6onNOPGGoTQCyzLrjo_cSCYY49oYZvqm1YjjrPtxFufk9ap7vYwHnAz6HLVT19EedDyqoK36-8XbL2ofvinJW8a7ugg8vxOI4euCKaursMTiQlK8BllMKlih-EqZGFKKOJ86MFA3Sag1CVWSULdJqBvpZ3_Odir5ZX0BuhX4jmOYk7HoDZ6wklUDQrK6LSdgg823_g9h8bmUvvj_0kKLlU6F8HuMvz_5j_l_AnMAt6U</recordid><startdate>20200701</startdate><enddate>20200701</enddate><creator>Zhu, Yuanyuan</creator><creator>Fan, Ze</creator><creator>Wang, Rui</creator><creator>Xie, Rougang</creator><creator>Guo, Haiyun</creator><creator>Zhang, Ming</creator><creator>Guo, Baolin</creator><creator>Sun, Tangna</creator><creator>Zhang, Haifeng</creator><creator>Zhuo, Lixia</creator><creator>Li, Yan</creator><creator>Wu, Shengxi</creator><general>Springer US</general><general>Springer Nature</general><general>Springer Nature B.V</general><scope>C6C</scope><scope>AOWDO</scope><scope>BLEPL</scope><scope>DTL</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>5PM</scope><orcidid>https://orcid.org/0000-0002-3210-9567</orcidid><orcidid>https://orcid.org/0000-0002-7465-7705</orcidid><orcidid>https://orcid.org/0000-0001-7644-1492</orcidid></search><sort><creationdate>20200701</creationdate><title>Single-Cell Analysis for Glycogen Localization and Metabolism in Cultured Astrocytes</title><author>Zhu, Yuanyuan ; Fan, Ze ; Wang, Rui ; Xie, Rougang ; Guo, Haiyun ; Zhang, Ming ; Guo, Baolin ; Sun, Tangna ; Zhang, Haifeng ; Zhuo, Lixia ; Li, Yan ; Wu, Shengxi</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c474t-ce8e059eac8096ccbad1fed251e7c3ee7cfa82b2c713112acc1c956aeebfa9bf3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2020</creationdate><topic>4-Chloro-7-nitrobenzofurazan - analogs & derivatives</topic><topic>4-Chloro-7-nitrobenzofurazan - chemistry</topic><topic>Animals</topic><topic>Astrocytes</topic><topic>Astrocytes - chemistry</topic><topic>Astrocytes - metabolism</topic><topic>Biomedical and Life Sciences</topic><topic>Biomedicine</topic><topic>Cell Biology</topic><topic>Cells, Cultured</topic><topic>Cerebral Cortex - metabolism</topic><topic>Deoxyglucose - analogs & derivatives</topic><topic>Deoxyglucose - chemistry</topic><topic>Flow cytometry</topic><topic>Glucose</topic><topic>Glycogen</topic><topic>Glycogen - analysis</topic><topic>Glycogen - deficiency</topic><topic>Glycogen - metabolism</topic><topic>Life Sciences & Biomedicine</topic><topic>Localization</topic><topic>Metabolism</topic><topic>Mice</topic><topic>Mice, Inbred C57BL</topic><topic>Neurobiology</topic><topic>Neurons - metabolism</topic><topic>Neurosciences</topic><topic>Neurosciences & Neurology</topic><topic>Original Research</topic><topic>Science & Technology</topic><topic>Single-Cell Analysis - methods</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Zhu, Yuanyuan</creatorcontrib><creatorcontrib>Fan, Ze</creatorcontrib><creatorcontrib>Wang, Rui</creatorcontrib><creatorcontrib>Xie, Rougang</creatorcontrib><creatorcontrib>Guo, Haiyun</creatorcontrib><creatorcontrib>Zhang, Ming</creatorcontrib><creatorcontrib>Guo, Baolin</creatorcontrib><creatorcontrib>Sun, Tangna</creatorcontrib><creatorcontrib>Zhang, Haifeng</creatorcontrib><creatorcontrib>Zhuo, Lixia</creatorcontrib><creatorcontrib>Li, Yan</creatorcontrib><creatorcontrib>Wu, Shengxi</creatorcontrib><collection>Springer Nature OA/Free Journals</collection><collection>Web of Science - Science Citation Index Expanded - 2020</collection><collection>Web of Science Core Collection</collection><collection>Science Citation Index Expanded</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Cellular and molecular neurobiology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Zhu, Yuanyuan</au><au>Fan, Ze</au><au>Wang, Rui</au><au>Xie, Rougang</au><au>Guo, Haiyun</au><au>Zhang, Ming</au><au>Guo, Baolin</au><au>Sun, Tangna</au><au>Zhang, Haifeng</au><au>Zhuo, Lixia</au><au>Li, Yan</au><au>Wu, Shengxi</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Single-Cell Analysis for Glycogen Localization and Metabolism in Cultured Astrocytes</atitle><jtitle>Cellular and molecular neurobiology</jtitle><stitle>Cell Mol Neurobiol</stitle><stitle>CELL MOL NEUROBIOL</stitle><addtitle>Cell Mol Neurobiol</addtitle><date>2020-07-01</date><risdate>2020</risdate><volume>40</volume><issue>5</issue><spage>801</spage><epage>812</epage><pages>801-812</pages><issn>0272-4340</issn><eissn>1573-6830</eissn><abstract>Cerebral glycogen is principally localized in astrocytes rather than in neurons. Glycogen metabolism has been implicated in higher brain functions, including learning and memory, yet the distribution patterns of glycogen in different types of astrocytes have not been fully described. Here, we applied a method based on the incorporation of 2-NBDG, a
d
-glucose fluorescent derivative that can trace glycogen, to investigate glycogen’s distribution in the brain. We identified two types of astrocytes, namely, 2-NBDG
I
(glycogen-deficient) and 2-NBDG
II
(glycogen-rich) cells. Whole-cell patch-clamp and fluorescence-activated cell sorting (FACS) were used to separate 2-NBDG
II
astrocytes from 2-NBDG
I
astrocytes. The expression levels of glycogen metabolic enzymes were analyzed in 2-NBDG
I
and 2-NBDG
II
astrocytes. We found unique glycogen metabolic patterns between 2-NBDG
I
and 2-NBDG
II
astrocytes. We also observed that 2-NBDG
II
astrocytes were mainly identified as fibrous astrocytes but not protoplasmic astrocytes. Our data reveal cell type-dependent glycogen distribution and metabolism patterns, suggesting diverse functions of these different astrocytes.</abstract><cop>New York</cop><pub>Springer US</pub><pmid>31863221</pmid><doi>10.1007/s10571-019-00775-4</doi><tpages>12</tpages><orcidid>https://orcid.org/0000-0002-3210-9567</orcidid><orcidid>https://orcid.org/0000-0002-7465-7705</orcidid><orcidid>https://orcid.org/0000-0001-7644-1492</orcidid><oa>free_for_read</oa></addata></record> |
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| subjects | 4-Chloro-7-nitrobenzofurazan - analogs & derivatives 4-Chloro-7-nitrobenzofurazan - chemistry Animals Astrocytes Astrocytes - chemistry Astrocytes - metabolism Biomedical and Life Sciences Biomedicine Cell Biology Cells, Cultured Cerebral Cortex - metabolism Deoxyglucose - analogs & derivatives Deoxyglucose - chemistry Flow cytometry Glucose Glycogen Glycogen - analysis Glycogen - deficiency Glycogen - metabolism Life Sciences & Biomedicine Localization Metabolism Mice Mice, Inbred C57BL Neurobiology Neurons - metabolism Neurosciences Neurosciences & Neurology Original Research Science & Technology Single-Cell Analysis - methods |
| title | Single-Cell Analysis for Glycogen Localization and Metabolism in Cultured Astrocytes |
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