Expression and functional analysis of P450 gene induced tolerance/resistance to lambda-cyhalothrin in quercetin fed larvae of beet armyworm Spodoptera exigua (Hübner)
Beet armyworm, Spodoptera exigua (Hübner) is an agronomical important and most devastating polyphagous pest that damages a variety of crops around the globe including China. Quercetin is one of the abundant dietary flavonoids and the important defense allelochemicals in plants. Therefore, the change...
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| Veröffentlicht in: | Saudi journal of biological sciences 2020-01, Vol.27 (1), p.77-87 |
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| creator | Hafeez, Muhammad Qasim, Muhammad Ali, Sajjad Yousaf, Hafiz Kamran Waqas, Muhammad Ali, Ehsan Ahmad, Muhammad Afaq Jan, Saad Bashir, Muhammad Amjad Noman, Ali Wang, Mo Gharmh, Hamed A. Khan, Khalid Ali |
| description | Beet armyworm, Spodoptera exigua (Hübner) is an agronomical important and most devastating polyphagous pest that damages a variety of crops around the globe including China. Quercetin is one of the abundant dietary flavonoids and the important defense allelochemicals in plants. Therefore, the changes in insect detoxification enzymes activities in response to plants allelochemicals may result increased the sensitivity to insecticides. In this study, we examined the induced effect of quercetin on larval tolerance to lambda-cyhalothrin in S. exigua. Application of cytochrome P450 inhibitor piperonyl butoxide (PBO) significantly synergized the lambda-cyhalothrin toxicity in quercetin-fed S. exigua larvae. Moreover, larval weight significantly reduced in quercetin, lambda-cyhalothrin, and quercetin + lambda-cyhalothrin treatment. Furthermore, our results showed that the P450 detoxification enzyme effectively increased in all treatments as compared to the control. Quantitative Real-time PCR analysis revealed that expression level of CYP6AE10 significantly upregulated in larvae treated with quercetin, lambda-cyhalothrin and quercetin + lambda-cyhalothrin in the midgut and fat body respectively. In addition, RNAi mediated knockdown of CYP6AE10 in S. exigua larvae significantly decreased the transcription level of target cytochrome P450 gene followed by the exposure with quercetin, lambda-cyhalothrin, and quercetin + lambda-cyhalothrin. Similarly, the knockdown of CYP6AE10 by the injection of dsRNA led to increased mortality after the treatment with respective chemicals. Overall, these data showed that P450s might possibly play an important role in the metabolic adaptation of S. exigua larvae to its host plant defense allelochemicals as well as insecticides. In conclusion, S. exigua can take benefit from its host plant’s secondary metabolites to elaborate its defense against synthetic insecticides. |
| doi_str_mv | 10.1016/j.sjbs.2019.05.005 |
| format | Article |
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Quercetin is one of the abundant dietary flavonoids and the important defense allelochemicals in plants. Therefore, the changes in insect detoxification enzymes activities in response to plants allelochemicals may result increased the sensitivity to insecticides. In this study, we examined the induced effect of quercetin on larval tolerance to lambda-cyhalothrin in S. exigua. Application of cytochrome P450 inhibitor piperonyl butoxide (PBO) significantly synergized the lambda-cyhalothrin toxicity in quercetin-fed S. exigua larvae. Moreover, larval weight significantly reduced in quercetin, lambda-cyhalothrin, and quercetin + lambda-cyhalothrin treatment. Furthermore, our results showed that the P450 detoxification enzyme effectively increased in all treatments as compared to the control. Quantitative Real-time PCR analysis revealed that expression level of CYP6AE10 significantly upregulated in larvae treated with quercetin, lambda-cyhalothrin and quercetin + lambda-cyhalothrin in the midgut and fat body respectively. In addition, RNAi mediated knockdown of CYP6AE10 in S. exigua larvae significantly decreased the transcription level of target cytochrome P450 gene followed by the exposure with quercetin, lambda-cyhalothrin, and quercetin + lambda-cyhalothrin. Similarly, the knockdown of CYP6AE10 by the injection of dsRNA led to increased mortality after the treatment with respective chemicals. Overall, these data showed that P450s might possibly play an important role in the metabolic adaptation of S. exigua larvae to its host plant defense allelochemicals as well as insecticides. In conclusion, S. exigua can take benefit from its host plant’s secondary metabolites to elaborate its defense against synthetic insecticides.</description><identifier>ISSN: 1319-562X</identifier><identifier>EISSN: 2213-7106</identifier><identifier>DOI: 10.1016/j.sjbs.2019.05.005</identifier><identifier>PMID: 31889821</identifier><language>eng</language><publisher>Riyadh, Saudi Arabia: Elsevier B.V</publisher><subject>Detoxification enzyme ; Lambda-cyhalothrin tolerance ; Midgut P450 genes ; Quercetin ; RNA interference ; The Beet armyworm ; الآثار الفسيولوجية ; التعبير الجيني ; التوافقية الحيوية ; الدودة الخضراء ; اليرقات ; مبيدات الآفات</subject><ispartof>Saudi journal of biological sciences, 2020-01, Vol.27 (1), p.77-87</ispartof><rights>2019</rights><rights>2019 Production and hosting by Elsevier B.V. on behalf of King Saud University.</rights><rights>2019 Production and hosting by Elsevier B.V. on behalf of King Saud University. 2019</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c477t-1643b10c9d8e46b2173feb60317a5957b862bfbbc0bf5fe37020ab1ea9c718a63</citedby><cites>FETCH-LOGICAL-c477t-1643b10c9d8e46b2173feb60317a5957b862bfbbc0bf5fe37020ab1ea9c718a63</cites><orcidid>0000-0002-4734-6338 ; 0000-0002-8063-9140</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC6933212/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.sjbs.2019.05.005$$EHTML$$P50$$Gelsevier$$Hfree_for_read</linktohtml><link.rule.ids>230,314,727,780,784,885,3550,27924,27925,45995,53791,53793</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/31889821$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Hafeez, Muhammad</creatorcontrib><creatorcontrib>Qasim, Muhammad</creatorcontrib><creatorcontrib>Ali, Sajjad</creatorcontrib><creatorcontrib>Yousaf, Hafiz Kamran</creatorcontrib><creatorcontrib>Waqas, Muhammad</creatorcontrib><creatorcontrib>Ali, Ehsan</creatorcontrib><creatorcontrib>Ahmad, Muhammad Afaq</creatorcontrib><creatorcontrib>Jan, Saad</creatorcontrib><creatorcontrib>Bashir, Muhammad Amjad</creatorcontrib><creatorcontrib>Noman, Ali</creatorcontrib><creatorcontrib>Wang, Mo</creatorcontrib><creatorcontrib>Gharmh, Hamed A.</creatorcontrib><creatorcontrib>Khan, Khalid Ali</creatorcontrib><title>Expression and functional analysis of P450 gene induced tolerance/resistance to lambda-cyhalothrin in quercetin fed larvae of beet armyworm Spodoptera exigua (Hübner)</title><title>Saudi journal of biological sciences</title><addtitle>Saudi J Biol Sci</addtitle><description>Beet armyworm, Spodoptera exigua (Hübner) is an agronomical important and most devastating polyphagous pest that damages a variety of crops around the globe including China. Quercetin is one of the abundant dietary flavonoids and the important defense allelochemicals in plants. Therefore, the changes in insect detoxification enzymes activities in response to plants allelochemicals may result increased the sensitivity to insecticides. In this study, we examined the induced effect of quercetin on larval tolerance to lambda-cyhalothrin in S. exigua. Application of cytochrome P450 inhibitor piperonyl butoxide (PBO) significantly synergized the lambda-cyhalothrin toxicity in quercetin-fed S. exigua larvae. Moreover, larval weight significantly reduced in quercetin, lambda-cyhalothrin, and quercetin + lambda-cyhalothrin treatment. Furthermore, our results showed that the P450 detoxification enzyme effectively increased in all treatments as compared to the control. Quantitative Real-time PCR analysis revealed that expression level of CYP6AE10 significantly upregulated in larvae treated with quercetin, lambda-cyhalothrin and quercetin + lambda-cyhalothrin in the midgut and fat body respectively. In addition, RNAi mediated knockdown of CYP6AE10 in S. exigua larvae significantly decreased the transcription level of target cytochrome P450 gene followed by the exposure with quercetin, lambda-cyhalothrin, and quercetin + lambda-cyhalothrin. Similarly, the knockdown of CYP6AE10 by the injection of dsRNA led to increased mortality after the treatment with respective chemicals. Overall, these data showed that P450s might possibly play an important role in the metabolic adaptation of S. exigua larvae to its host plant defense allelochemicals as well as insecticides. In conclusion, S. exigua can take benefit from its host plant’s secondary metabolites to elaborate its defense against synthetic insecticides.</description><subject>Detoxification enzyme</subject><subject>Lambda-cyhalothrin tolerance</subject><subject>Midgut P450 genes</subject><subject>Quercetin</subject><subject>RNA interference</subject><subject>The Beet armyworm</subject><subject>الآثار الفسيولوجية</subject><subject>التعبير الجيني</subject><subject>التوافقية الحيوية</subject><subject>الدودة الخضراء</subject><subject>اليرقات</subject><subject>مبيدات الآفات</subject><issn>1319-562X</issn><issn>2213-7106</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2020</creationdate><recordtype>article</recordtype><recordid>eNp9Ud2K1DAULqK44-oLCEou14t289OmLciCLKsrLCio4F1I0tOZDG1Sk3TceSJfwjtfzJQZB73xKuec7-ck-bLsOcEFwYRfbouwVaGgmLQFrgqMqwfZilLC8ppg_jBbEUbavOL061n2JIQtxrxhDXmcnTHSNG1DySr7cXM_eQjBOIuk7VA_Wx1TI4fUymEfTECuRx_LCqM1WEDGdrOGDkU3gJdWw2WSmxCXMg3RIEfVyVzvN3JwceONTRL0bQavIaaqT9pB-p2ExVcBRCT9uP_u_Ig-Ta5zU0y-CO7Nepbo4vbXT2XBv3qaPerlEODZ8TzPvry9-Xx9m999ePf--s1drsu6jjnhJVME67ZroOSKkpr1oDhmpJZVW9Wq4VT1Smms-qoHVmOKpSIgW12TRnJ2nl0dfKdZjdBpsNHLQUzejNLvhZNG_ItYsxFrtxO8ZYwSmgwujgbepVeHKEYTNAyDtODmIChjhDPalGWi0gNVexeCh_60hmCxJCy2YklYLAkLXImUcBK9_PuCJ8mfSBPhxYEAaQ69PDFaikvOEv76iKdv3BnwImgDKb3OeNBRdM78b_9vTf3HCQ</recordid><startdate>20200101</startdate><enddate>20200101</enddate><creator>Hafeez, Muhammad</creator><creator>Qasim, Muhammad</creator><creator>Ali, Sajjad</creator><creator>Yousaf, Hafiz Kamran</creator><creator>Waqas, Muhammad</creator><creator>Ali, Ehsan</creator><creator>Ahmad, Muhammad Afaq</creator><creator>Jan, Saad</creator><creator>Bashir, Muhammad Amjad</creator><creator>Noman, Ali</creator><creator>Wang, Mo</creator><creator>Gharmh, Hamed A.</creator><creator>Khan, Khalid Ali</creator><general>Elsevier B.V</general><general>Saudi Biological Society</general><general>Elsevier</general><scope>6I.</scope><scope>AAFTH</scope><scope>ADJCN</scope><scope>AHFXO</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>5PM</scope><orcidid>https://orcid.org/0000-0002-4734-6338</orcidid><orcidid>https://orcid.org/0000-0002-8063-9140</orcidid></search><sort><creationdate>20200101</creationdate><title>Expression and functional analysis of P450 gene induced tolerance/resistance to lambda-cyhalothrin in quercetin fed larvae of beet armyworm Spodoptera exigua (Hübner)</title><author>Hafeez, Muhammad ; Qasim, Muhammad ; Ali, Sajjad ; Yousaf, Hafiz Kamran ; Waqas, Muhammad ; Ali, Ehsan ; Ahmad, Muhammad Afaq ; Jan, Saad ; Bashir, Muhammad Amjad ; Noman, Ali ; Wang, Mo ; Gharmh, Hamed A. ; Khan, Khalid Ali</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c477t-1643b10c9d8e46b2173feb60317a5957b862bfbbc0bf5fe37020ab1ea9c718a63</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2020</creationdate><topic>Detoxification enzyme</topic><topic>Lambda-cyhalothrin tolerance</topic><topic>Midgut P450 genes</topic><topic>Quercetin</topic><topic>RNA interference</topic><topic>The Beet armyworm</topic><topic>الآثار الفسيولوجية</topic><topic>التعبير الجيني</topic><topic>التوافقية الحيوية</topic><topic>الدودة الخضراء</topic><topic>اليرقات</topic><topic>مبيدات الآفات</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Hafeez, Muhammad</creatorcontrib><creatorcontrib>Qasim, Muhammad</creatorcontrib><creatorcontrib>Ali, Sajjad</creatorcontrib><creatorcontrib>Yousaf, Hafiz Kamran</creatorcontrib><creatorcontrib>Waqas, Muhammad</creatorcontrib><creatorcontrib>Ali, Ehsan</creatorcontrib><creatorcontrib>Ahmad, Muhammad Afaq</creatorcontrib><creatorcontrib>Jan, Saad</creatorcontrib><creatorcontrib>Bashir, Muhammad Amjad</creatorcontrib><creatorcontrib>Noman, Ali</creatorcontrib><creatorcontrib>Wang, Mo</creatorcontrib><creatorcontrib>Gharmh, Hamed A.</creatorcontrib><creatorcontrib>Khan, Khalid Ali</creatorcontrib><collection>ScienceDirect Open Access Titles</collection><collection>Elsevier:ScienceDirect:Open Access</collection><collection>الدوريات العلمية والإحصائية - e-Marefa Academic and Statistical Periodicals</collection><collection>معرفة - المحتوى العربي الأكاديمي المتكامل - e-Marefa Academic Complete</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Saudi journal of biological sciences</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Hafeez, Muhammad</au><au>Qasim, Muhammad</au><au>Ali, Sajjad</au><au>Yousaf, Hafiz Kamran</au><au>Waqas, Muhammad</au><au>Ali, Ehsan</au><au>Ahmad, Muhammad Afaq</au><au>Jan, Saad</au><au>Bashir, Muhammad Amjad</au><au>Noman, Ali</au><au>Wang, Mo</au><au>Gharmh, Hamed A.</au><au>Khan, Khalid Ali</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Expression and functional analysis of P450 gene induced tolerance/resistance to lambda-cyhalothrin in quercetin fed larvae of beet armyworm Spodoptera exigua (Hübner)</atitle><jtitle>Saudi journal of biological sciences</jtitle><addtitle>Saudi J Biol Sci</addtitle><date>2020-01-01</date><risdate>2020</risdate><volume>27</volume><issue>1</issue><spage>77</spage><epage>87</epage><pages>77-87</pages><issn>1319-562X</issn><eissn>2213-7106</eissn><abstract>Beet armyworm, Spodoptera exigua (Hübner) is an agronomical important and most devastating polyphagous pest that damages a variety of crops around the globe including China. Quercetin is one of the abundant dietary flavonoids and the important defense allelochemicals in plants. Therefore, the changes in insect detoxification enzymes activities in response to plants allelochemicals may result increased the sensitivity to insecticides. In this study, we examined the induced effect of quercetin on larval tolerance to lambda-cyhalothrin in S. exigua. Application of cytochrome P450 inhibitor piperonyl butoxide (PBO) significantly synergized the lambda-cyhalothrin toxicity in quercetin-fed S. exigua larvae. Moreover, larval weight significantly reduced in quercetin, lambda-cyhalothrin, and quercetin + lambda-cyhalothrin treatment. Furthermore, our results showed that the P450 detoxification enzyme effectively increased in all treatments as compared to the control. Quantitative Real-time PCR analysis revealed that expression level of CYP6AE10 significantly upregulated in larvae treated with quercetin, lambda-cyhalothrin and quercetin + lambda-cyhalothrin in the midgut and fat body respectively. In addition, RNAi mediated knockdown of CYP6AE10 in S. exigua larvae significantly decreased the transcription level of target cytochrome P450 gene followed by the exposure with quercetin, lambda-cyhalothrin, and quercetin + lambda-cyhalothrin. Similarly, the knockdown of CYP6AE10 by the injection of dsRNA led to increased mortality after the treatment with respective chemicals. Overall, these data showed that P450s might possibly play an important role in the metabolic adaptation of S. exigua larvae to its host plant defense allelochemicals as well as insecticides. In conclusion, S. exigua can take benefit from its host plant’s secondary metabolites to elaborate its defense against synthetic insecticides.</abstract><cop>Riyadh, Saudi Arabia</cop><pub>Elsevier B.V</pub><pmid>31889821</pmid><doi>10.1016/j.sjbs.2019.05.005</doi><tpages>11</tpages><orcidid>https://orcid.org/0000-0002-4734-6338</orcidid><orcidid>https://orcid.org/0000-0002-8063-9140</orcidid><oa>free_for_read</oa></addata></record> |
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| ispartof | Saudi journal of biological sciences, 2020-01, Vol.27 (1), p.77-87 |
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| source | ScienceDirect Journals (5 years ago - present); EZB-FREE-00999 freely available EZB journals; PubMed Central |
| subjects | Detoxification enzyme Lambda-cyhalothrin tolerance Midgut P450 genes Quercetin RNA interference The Beet armyworm الآثار الفسيولوجية التعبير الجيني التوافقية الحيوية الدودة الخضراء اليرقات مبيدات الآفات |
| title | Expression and functional analysis of P450 gene induced tolerance/resistance to lambda-cyhalothrin in quercetin fed larvae of beet armyworm Spodoptera exigua (Hübner) |
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