RING‐box proteins regulate leaf senescence and stomatal closure via repression of ABA transporter gene ABCG40
ABSTRACT Plant hormone abscisic acid (ABA) plays an indispensable role in the control of leaf senescence, during which ABA signaling depends on its biosynthesis. Nevertheless, the role of ABA transport in leaf senescence remains unknown. Here, we identified two novel RING‐box protein‐encoding genes...
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| Veröffentlicht in: | Journal of integrative plant biology 2022-05, Vol.64 (5), p.979-994 |
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| container_title | Journal of integrative plant biology |
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| creator | Wei, Yun Qi Yuan, Jun Jie Xiao, Chen Chen Li, Gui Xin Yan, Jing Ying Zheng, Shao Jian Ding, Zhong Jie |
| description | ABSTRACT
Plant hormone abscisic acid (ABA) plays an indispensable role in the control of leaf senescence, during which ABA signaling depends on its biosynthesis. Nevertheless, the role of ABA transport in leaf senescence remains unknown. Here, we identified two novel RING‐box protein‐encoding genes UBIQUITIN LIGASE of SENESCENCE 1 and 2 (ULS1 and ULS2) involved in leaf senescence. Lack of ULS1 and ULS2 accelerates leaf senescence, which is specifically promoted by ABA treatment. Furthermore, the expression of senescence‐related genes is significantly affected in mature leaves of uls1/uls2 double mutant (versus wild type (WT)) in an ABA‐dependent manner, and the ABA content is substantially increased. ULS1 and ULS2 are mainly expressed in the guard cells and aging leaves, and the expression is induced by ABA. Further RNA‐seq and quantitative proteomics of ubiquitination reveal that ABA transporter ABCG40 is highly expressed in uls1/uls2 mutant versus WT, though it is not the direct target of ULS1/2. Finally, we show that the acceleration of leaf senescence, the increase of leaf ABA content, and the promotion of stomatal closure in uls1/usl2 mutant are suppressed by abcg40 loss‐of‐function mutation. These results indicate that ULS1 and ULS2 function in feedback inhibition of ABCG40‐dependent ABA transport during ABA‐induced leaf senescence and stomatal closure.
The RING‐box proteins UBIQUITIN LIGASE of SENESCENCE 1 and 2 function in feedback inhibition of ABCG40‐dependent abscisic acid (ABA) transport during ABA‐induced leaf senescence and stomatal closure. |
| doi_str_mv | 10.1111/jipb.13247 |
| format | Article |
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Plant hormone abscisic acid (ABA) plays an indispensable role in the control of leaf senescence, during which ABA signaling depends on its biosynthesis. Nevertheless, the role of ABA transport in leaf senescence remains unknown. Here, we identified two novel RING‐box protein‐encoding genes UBIQUITIN LIGASE of SENESCENCE 1 and 2 (ULS1 and ULS2) involved in leaf senescence. Lack of ULS1 and ULS2 accelerates leaf senescence, which is specifically promoted by ABA treatment. Furthermore, the expression of senescence‐related genes is significantly affected in mature leaves of uls1/uls2 double mutant (versus wild type (WT)) in an ABA‐dependent manner, and the ABA content is substantially increased. ULS1 and ULS2 are mainly expressed in the guard cells and aging leaves, and the expression is induced by ABA. Further RNA‐seq and quantitative proteomics of ubiquitination reveal that ABA transporter ABCG40 is highly expressed in uls1/uls2 mutant versus WT, though it is not the direct target of ULS1/2. Finally, we show that the acceleration of leaf senescence, the increase of leaf ABA content, and the promotion of stomatal closure in uls1/usl2 mutant are suppressed by abcg40 loss‐of‐function mutation. These results indicate that ULS1 and ULS2 function in feedback inhibition of ABCG40‐dependent ABA transport during ABA‐induced leaf senescence and stomatal closure.
The RING‐box proteins UBIQUITIN LIGASE of SENESCENCE 1 and 2 function in feedback inhibition of ABCG40‐dependent abscisic acid (ABA) transport during ABA‐induced leaf senescence and stomatal closure.</description><identifier>ISSN: 1672-9072</identifier><identifier>EISSN: 1744-7909</identifier><identifier>DOI: 10.1111/jipb.13247</identifier><identifier>PMID: 35274464</identifier><language>eng</language><publisher>China (Republic : 1949- ): Wiley Subscription Services, Inc</publisher><subject>ABA ; Abscisic acid ; Abscisic Acid - metabolism ; Abscisic Acid - pharmacology ; Aging ; Arabidopsis ; Arabidopsis - metabolism ; Arabidopsis Proteins - metabolism ; Biosynthesis ; E3 ligase ; Feedback inhibition ; Gene expression ; Genes ; Guard cells ; leaf ; Leaves ; Membrane Transport Proteins - metabolism ; Mutants ; Mutation ; Mutation - genetics ; Plant hormones ; Plant Leaves - metabolism ; Plant Senescence ; Plant Stomata - physiology ; Proteins ; Proteomics ; Senescence ; Stomata ; transport ; Ubiquitin ; Ubiquitin-protein ligase ; Ubiquitination</subject><ispartof>Journal of integrative plant biology, 2022-05, Vol.64 (5), p.979-994</ispartof><rights>2022 Institute of Botany, Chinese Academy of Sciences</rights><rights>2022 Institute of Botany, Chinese Academy of Sciences.</rights><rights>Copyright © Wanfang Data Co. Ltd. All Rights Reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c3877-a0d371e6e3d1c7b29e49f7d89a862c830c9315da4c982738301e0f82808dcde3</citedby><cites>FETCH-LOGICAL-c3877-a0d371e6e3d1c7b29e49f7d89a862c830c9315da4c982738301e0f82808dcde3</cites><orcidid>0000-0002-3336-8165 ; 0000-0003-1518-5143</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Uhttp://www.wanfangdata.com.cn/images/PeriodicalImages/zwxb/zwxb.jpg</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1111%2Fjipb.13247$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1111%2Fjipb.13247$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,776,780,1411,27901,27902,45550,45551</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/35274464$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Wei, Yun Qi</creatorcontrib><creatorcontrib>Yuan, Jun Jie</creatorcontrib><creatorcontrib>Xiao, Chen Chen</creatorcontrib><creatorcontrib>Li, Gui Xin</creatorcontrib><creatorcontrib>Yan, Jing Ying</creatorcontrib><creatorcontrib>Zheng, Shao Jian</creatorcontrib><creatorcontrib>Ding, Zhong Jie</creatorcontrib><title>RING‐box proteins regulate leaf senescence and stomatal closure via repression of ABA transporter gene ABCG40</title><title>Journal of integrative plant biology</title><addtitle>J Integr Plant Biol</addtitle><description>ABSTRACT
Plant hormone abscisic acid (ABA) plays an indispensable role in the control of leaf senescence, during which ABA signaling depends on its biosynthesis. Nevertheless, the role of ABA transport in leaf senescence remains unknown. Here, we identified two novel RING‐box protein‐encoding genes UBIQUITIN LIGASE of SENESCENCE 1 and 2 (ULS1 and ULS2) involved in leaf senescence. Lack of ULS1 and ULS2 accelerates leaf senescence, which is specifically promoted by ABA treatment. Furthermore, the expression of senescence‐related genes is significantly affected in mature leaves of uls1/uls2 double mutant (versus wild type (WT)) in an ABA‐dependent manner, and the ABA content is substantially increased. ULS1 and ULS2 are mainly expressed in the guard cells and aging leaves, and the expression is induced by ABA. Further RNA‐seq and quantitative proteomics of ubiquitination reveal that ABA transporter ABCG40 is highly expressed in uls1/uls2 mutant versus WT, though it is not the direct target of ULS1/2. Finally, we show that the acceleration of leaf senescence, the increase of leaf ABA content, and the promotion of stomatal closure in uls1/usl2 mutant are suppressed by abcg40 loss‐of‐function mutation. These results indicate that ULS1 and ULS2 function in feedback inhibition of ABCG40‐dependent ABA transport during ABA‐induced leaf senescence and stomatal closure.
The RING‐box proteins UBIQUITIN LIGASE of SENESCENCE 1 and 2 function in feedback inhibition of ABCG40‐dependent abscisic acid (ABA) transport during ABA‐induced leaf senescence and stomatal closure.</description><subject>ABA</subject><subject>Abscisic acid</subject><subject>Abscisic Acid - metabolism</subject><subject>Abscisic Acid - pharmacology</subject><subject>Aging</subject><subject>Arabidopsis</subject><subject>Arabidopsis - metabolism</subject><subject>Arabidopsis Proteins - metabolism</subject><subject>Biosynthesis</subject><subject>E3 ligase</subject><subject>Feedback inhibition</subject><subject>Gene expression</subject><subject>Genes</subject><subject>Guard cells</subject><subject>leaf</subject><subject>Leaves</subject><subject>Membrane Transport Proteins - metabolism</subject><subject>Mutants</subject><subject>Mutation</subject><subject>Mutation - genetics</subject><subject>Plant hormones</subject><subject>Plant Leaves - metabolism</subject><subject>Plant Senescence</subject><subject>Plant Stomata - physiology</subject><subject>Proteins</subject><subject>Proteomics</subject><subject>Senescence</subject><subject>Stomata</subject><subject>transport</subject><subject>Ubiquitin</subject><subject>Ubiquitin-protein ligase</subject><subject>Ubiquitination</subject><issn>1672-9072</issn><issn>1744-7909</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2022</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kcuKFDEUhoMozkU3PoAERBChxtyqklr2NNq2DCoy-5BKnWqqqU7KpGouruYRfEafxDN2OwsXZpNw-PKdnPyEvODsjON6t-3H5oxLofQjcsy1UoWuWf0Yz5UWRc20OCInOW8Zk4ZV4ik5kqVArFLHJH5bf179uvvZxBs6pjhBHzJNsJkHNwEdwHU0Q4DsIXigLrQ0T3HnJjdQP8Q8J6BXvcMbY4Kc-xho7OjifEGn5EIeY5og0Q0asLhcKfaMPOnckOH5YT8llx_eXy4_FhdfVuvl4qLw0mhdONZKzaEC2XKvG1GDqjvdmtqZSngjma8lL1unfG2ElljgwDojDDOtb0Gektd77bULnQsbu41zCtjQ_ri-aQQTgpX4Hci92XM4-_cZ8mR3Pc46DC5AnLMVFT6Hq9IIRF_9gz44RVUpXSrJNFJv95RPMecEnR1Tv3Pp1nJm79Oy92nZP2kh_PKgnJsdtA_o33gQ4Icx-gFu_6Oyn9Zfz_fS34LRnok</recordid><startdate>202205</startdate><enddate>202205</enddate><creator>Wei, Yun Qi</creator><creator>Yuan, Jun Jie</creator><creator>Xiao, Chen Chen</creator><creator>Li, Gui Xin</creator><creator>Yan, Jing Ying</creator><creator>Zheng, Shao Jian</creator><creator>Ding, Zhong Jie</creator><general>Wiley Subscription Services, Inc</general><general>State Key Laboratory of Plant Physiology and Biochemistry,College of Life Sciences,Zhejiang University,Hangzhou 310058,China</general><general>Guangdong Laboratory for Lingnan Modern Agriculture,College of Natural Resources and Environment,South China Agricultural University,Guangzhou 510642,China%State Key Laboratory of Plant Physiology and Biochemistry,College of Life Sciences,Zhejiang University,Hangzhou 310058,China%College of Agronomy and Biotechnology,Zhejiang University,Hangzhou 310058,China%Agricultural Experimental Station,Zhejiang University,Hangzhou 310058,China</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QO</scope><scope>7T7</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope><scope>2B.</scope><scope>4A8</scope><scope>92I</scope><scope>93N</scope><scope>PSX</scope><scope>TCJ</scope><orcidid>https://orcid.org/0000-0002-3336-8165</orcidid><orcidid>https://orcid.org/0000-0003-1518-5143</orcidid></search><sort><creationdate>202205</creationdate><title>RING‐box proteins regulate leaf senescence and stomatal closure via repression of ABA transporter gene ABCG40</title><author>Wei, Yun Qi ; Yuan, Jun Jie ; Xiao, Chen Chen ; Li, Gui Xin ; Yan, Jing Ying ; Zheng, Shao Jian ; Ding, Zhong Jie</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c3877-a0d371e6e3d1c7b29e49f7d89a862c830c9315da4c982738301e0f82808dcde3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2022</creationdate><topic>ABA</topic><topic>Abscisic acid</topic><topic>Abscisic Acid - metabolism</topic><topic>Abscisic Acid - pharmacology</topic><topic>Aging</topic><topic>Arabidopsis</topic><topic>Arabidopsis - metabolism</topic><topic>Arabidopsis Proteins - metabolism</topic><topic>Biosynthesis</topic><topic>E3 ligase</topic><topic>Feedback inhibition</topic><topic>Gene expression</topic><topic>Genes</topic><topic>Guard cells</topic><topic>leaf</topic><topic>Leaves</topic><topic>Membrane Transport Proteins - metabolism</topic><topic>Mutants</topic><topic>Mutation</topic><topic>Mutation - genetics</topic><topic>Plant hormones</topic><topic>Plant Leaves - metabolism</topic><topic>Plant Senescence</topic><topic>Plant Stomata - physiology</topic><topic>Proteins</topic><topic>Proteomics</topic><topic>Senescence</topic><topic>Stomata</topic><topic>transport</topic><topic>Ubiquitin</topic><topic>Ubiquitin-protein ligase</topic><topic>Ubiquitination</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Wei, Yun Qi</creatorcontrib><creatorcontrib>Yuan, Jun Jie</creatorcontrib><creatorcontrib>Xiao, Chen Chen</creatorcontrib><creatorcontrib>Li, Gui Xin</creatorcontrib><creatorcontrib>Yan, Jing Ying</creatorcontrib><creatorcontrib>Zheng, Shao Jian</creatorcontrib><creatorcontrib>Ding, Zhong Jie</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Biotechnology Research Abstracts</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><collection>Wanfang Data Journals - Hong Kong</collection><collection>WANFANG Data Centre</collection><collection>Wanfang Data Journals</collection><collection>万方数据期刊 - 香港版</collection><collection>China Online Journals (COJ)</collection><collection>China Online Journals (COJ)</collection><jtitle>Journal of integrative plant biology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Wei, Yun Qi</au><au>Yuan, Jun Jie</au><au>Xiao, Chen Chen</au><au>Li, Gui Xin</au><au>Yan, Jing Ying</au><au>Zheng, Shao Jian</au><au>Ding, Zhong Jie</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>RING‐box proteins regulate leaf senescence and stomatal closure via repression of ABA transporter gene ABCG40</atitle><jtitle>Journal of integrative plant biology</jtitle><addtitle>J Integr Plant Biol</addtitle><date>2022-05</date><risdate>2022</risdate><volume>64</volume><issue>5</issue><spage>979</spage><epage>994</epage><pages>979-994</pages><issn>1672-9072</issn><eissn>1744-7909</eissn><abstract>ABSTRACT
Plant hormone abscisic acid (ABA) plays an indispensable role in the control of leaf senescence, during which ABA signaling depends on its biosynthesis. Nevertheless, the role of ABA transport in leaf senescence remains unknown. Here, we identified two novel RING‐box protein‐encoding genes UBIQUITIN LIGASE of SENESCENCE 1 and 2 (ULS1 and ULS2) involved in leaf senescence. Lack of ULS1 and ULS2 accelerates leaf senescence, which is specifically promoted by ABA treatment. Furthermore, the expression of senescence‐related genes is significantly affected in mature leaves of uls1/uls2 double mutant (versus wild type (WT)) in an ABA‐dependent manner, and the ABA content is substantially increased. ULS1 and ULS2 are mainly expressed in the guard cells and aging leaves, and the expression is induced by ABA. Further RNA‐seq and quantitative proteomics of ubiquitination reveal that ABA transporter ABCG40 is highly expressed in uls1/uls2 mutant versus WT, though it is not the direct target of ULS1/2. Finally, we show that the acceleration of leaf senescence, the increase of leaf ABA content, and the promotion of stomatal closure in uls1/usl2 mutant are suppressed by abcg40 loss‐of‐function mutation. These results indicate that ULS1 and ULS2 function in feedback inhibition of ABCG40‐dependent ABA transport during ABA‐induced leaf senescence and stomatal closure.
The RING‐box proteins UBIQUITIN LIGASE of SENESCENCE 1 and 2 function in feedback inhibition of ABCG40‐dependent abscisic acid (ABA) transport during ABA‐induced leaf senescence and stomatal closure.</abstract><cop>China (Republic : 1949- )</cop><pub>Wiley Subscription Services, Inc</pub><pmid>35274464</pmid><doi>10.1111/jipb.13247</doi><tpages>16</tpages><orcidid>https://orcid.org/0000-0002-3336-8165</orcidid><orcidid>https://orcid.org/0000-0003-1518-5143</orcidid></addata></record> |
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| subjects | ABA Abscisic acid Abscisic Acid - metabolism Abscisic Acid - pharmacology Aging Arabidopsis Arabidopsis - metabolism Arabidopsis Proteins - metabolism Biosynthesis E3 ligase Feedback inhibition Gene expression Genes Guard cells leaf Leaves Membrane Transport Proteins - metabolism Mutants Mutation Mutation - genetics Plant hormones Plant Leaves - metabolism Plant Senescence Plant Stomata - physiology Proteins Proteomics Senescence Stomata transport Ubiquitin Ubiquitin-protein ligase Ubiquitination |
| title | RING‐box proteins regulate leaf senescence and stomatal closure via repression of ABA transporter gene ABCG40 |
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