Nicotiana benthamiana LRR‐RLP NbEIX2 mediates the perception of an EIX‐like protein from Verticillium dahliae
Verticillium wilt diseases caused by the soil‐borne fungus Verticillium dahliae result in devastating yield losses in many economically important crops annually. Here, we identified a novel ethylene‐inducing xylanase (EIX)‐like protein, VdEIX3, from V. dahliae, which exhibits immunity‐inducing activ...
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| Veröffentlicht in: | Journal of integrative plant biology 2021-05, Vol.63 (5), p.949-960 |
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| creator | Yin, Zhiyuan Wang, Nan Pi, Lei Li, Lei Duan, Weiwei Wang, Xiaodan Dou, Daolong |
| description | Verticillium wilt diseases caused by the soil‐borne fungus Verticillium dahliae result in devastating yield losses in many economically important crops annually. Here, we identified a novel ethylene‐inducing xylanase (EIX)‐like protein, VdEIX3, from V. dahliae, which exhibits immunity‐inducing activity in Nicotiana benthamiana. In vitro‐purified VdEIX3 can induce strong oxidative burst, activate the expression of defense‐related genes, and increase resistance against oomycete and fungal pathogens in N. benthamiana. VdEIX3 orthologs of other Verticillium pathogens also induce cell death in N. benthamiana, which form a new type of EIX protein family that is distinct from the known EIX proteins. A leucine‐rich repeat receptor‐like protein, NbEIX2, regulates the perception of VdEIX3 in N. benthamiana. Our results demonstrate that VdEIX3 is a novel EIX‐like protein that can be recognized by N. benthamiana NbEIX2, and also suggest that NbEIX2 is a promising receptor‐like protein that is potentially applicable to transgenic breeding for improving resistance to Verticillium wilt diseases.
Tomato plants detect Ethylene‐inducing xylanase (EIX) from the fungus Trichoderma viride and activate defense responses, but Nicotiana benthamiana plants do not detect EIX. Here, we identified an EIX‐like protein from the soil‐borne fungus Verticillium dahliae, VdEIX3, which is detected by the N. benthamiana receptor‐like protein NbEIX2. |
| doi_str_mv | 10.1111/jipb.13031 |
| format | Article |
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Tomato plants detect Ethylene‐inducing xylanase (EIX) from the fungus Trichoderma viride and activate defense responses, but Nicotiana benthamiana plants do not detect EIX. Here, we identified an EIX‐like protein from the soil‐borne fungus Verticillium dahliae, VdEIX3, which is detected by the N. benthamiana receptor‐like protein NbEIX2.</description><identifier>ISSN: 1672-9072</identifier><identifier>EISSN: 1744-7909</identifier><identifier>DOI: 10.1111/jipb.13031</identifier><identifier>PMID: 33205907</identifier><language>eng</language><publisher>China (Republic : 1949- ): Wiley Subscription Services, Inc</publisher><subject>Breeding ; Cell death ; ethylene‐inducing xylanase (EIX) ; Gene expression ; Leucine ; microbe‐associated molecular pattern (MAMP) ; Nicotiana benthamiana ; Pathogens ; Perception ; Proteins ; PRR ; Receptors ; Soil microorganisms ; Verticillium dahliae ; Verticillium wilt ; Xylanase</subject><ispartof>Journal of integrative plant biology, 2021-05, Vol.63 (5), p.949-960</ispartof><rights>2020 Institute of Botany, Chinese Academy of Sciences</rights><rights>2020 Institute of Botany, Chinese Academy of Sciences.</rights><rights>2021 Institute of Botany, Chinese Academy of Sciences</rights><rights>Copyright © Wanfang Data Co. Ltd. All Rights Reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c3871-91e682b9a137546dbb4e08b62b06e6e847f21ab5c4ea949a92970c1bc2c7eb173</citedby><cites>FETCH-LOGICAL-c3871-91e682b9a137546dbb4e08b62b06e6e847f21ab5c4ea949a92970c1bc2c7eb173</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Uhttp://www.wanfangdata.com.cn/images/PeriodicalImages/zwxb/zwxb.jpg</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1111%2Fjipb.13031$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1111%2Fjipb.13031$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,780,784,1417,27924,27925,45574,45575</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/33205907$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Yin, Zhiyuan</creatorcontrib><creatorcontrib>Wang, Nan</creatorcontrib><creatorcontrib>Pi, Lei</creatorcontrib><creatorcontrib>Li, Lei</creatorcontrib><creatorcontrib>Duan, Weiwei</creatorcontrib><creatorcontrib>Wang, Xiaodan</creatorcontrib><creatorcontrib>Dou, Daolong</creatorcontrib><title>Nicotiana benthamiana LRR‐RLP NbEIX2 mediates the perception of an EIX‐like protein from Verticillium dahliae</title><title>Journal of integrative plant biology</title><addtitle>J Integr Plant Biol</addtitle><description>Verticillium wilt diseases caused by the soil‐borne fungus Verticillium dahliae result in devastating yield losses in many economically important crops annually. Here, we identified a novel ethylene‐inducing xylanase (EIX)‐like protein, VdEIX3, from V. dahliae, which exhibits immunity‐inducing activity in Nicotiana benthamiana. In vitro‐purified VdEIX3 can induce strong oxidative burst, activate the expression of defense‐related genes, and increase resistance against oomycete and fungal pathogens in N. benthamiana. VdEIX3 orthologs of other Verticillium pathogens also induce cell death in N. benthamiana, which form a new type of EIX protein family that is distinct from the known EIX proteins. A leucine‐rich repeat receptor‐like protein, NbEIX2, regulates the perception of VdEIX3 in N. benthamiana. Our results demonstrate that VdEIX3 is a novel EIX‐like protein that can be recognized by N. benthamiana NbEIX2, and also suggest that NbEIX2 is a promising receptor‐like protein that is potentially applicable to transgenic breeding for improving resistance to Verticillium wilt diseases.
Tomato plants detect Ethylene‐inducing xylanase (EIX) from the fungus Trichoderma viride and activate defense responses, but Nicotiana benthamiana plants do not detect EIX. Here, we identified an EIX‐like protein from the soil‐borne fungus Verticillium dahliae, VdEIX3, which is detected by the N. benthamiana receptor‐like protein NbEIX2.</description><subject>Breeding</subject><subject>Cell death</subject><subject>ethylene‐inducing xylanase (EIX)</subject><subject>Gene expression</subject><subject>Leucine</subject><subject>microbe‐associated molecular pattern (MAMP)</subject><subject>Nicotiana benthamiana</subject><subject>Pathogens</subject><subject>Perception</subject><subject>Proteins</subject><subject>PRR</subject><subject>Receptors</subject><subject>Soil microorganisms</subject><subject>Verticillium dahliae</subject><subject>Verticillium wilt</subject><subject>Xylanase</subject><issn>1672-9072</issn><issn>1744-7909</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2021</creationdate><recordtype>article</recordtype><recordid>eNp9kd1qFDEUx4NY-mVvfAAJiCCFqfmYSSaXWmpdWWpZWvEuJNkzbtaZzDaZoa1XPoLP6JM021174YW5OQfOj1-S80foJSUnNJ93S7-yJ5QTTp-hfSrLspCKqOe5F5IViki2hw5SWhLCayLYLtrjnJEqD_bRzYV3_eBNMNhCGBame-yns9mfX79n00t8Yc8m3xjuYO7NAAkPC8AriA5Wg-8D7htsAs5Ixlv_I89iP4APuIl9h79CHLzzbevHDs_NovUGXqCdxrQJjrb1EF1_PLs6_VRMv5xPTt9PC8drSQtFQdTMKkO5rEoxt7YEUlvBLBEgoC5lw6ixlSvBqFIZxZQkjlrHnARLJT9EbzbeWxMaE77rZT_GkG_UP2_vLCOMkopQnrm3Gy6__GaENOjOJwdtawL0Y9KsFLQWrK7X6Ot_0Ccnq7JPZU5l6nhDudinFKHRq-g7E-81JXodmF4Hph8Dy_CrrXK0ecdP6N-EMkC33_At3P9HpT9PLj9spA9I_aBJ</recordid><startdate>202105</startdate><enddate>202105</enddate><creator>Yin, Zhiyuan</creator><creator>Wang, Nan</creator><creator>Pi, Lei</creator><creator>Li, Lei</creator><creator>Duan, Weiwei</creator><creator>Wang, Xiaodan</creator><creator>Dou, Daolong</creator><general>Wiley Subscription Services, Inc</general><general>College of Plant Protection, Nanjing Agricultural University, Nanjing 210095, China</general><general>College of Agriculture, Northeast Agricultural University, Harbin 150030, China%College of Plant Protection, Nanjing Agricultural University, Nanjing 210095, China%College of Plant Protection, China Agricultural University, Beijing 100193, China</general><general>College of Plant Protection, China Agricultural University, Beijing 100193, China%College of Plant Protection, China Agricultural University, Beijing 100193, China</general><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QO</scope><scope>7T7</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope><scope>2B.</scope><scope>4A8</scope><scope>92I</scope><scope>93N</scope><scope>PSX</scope><scope>TCJ</scope></search><sort><creationdate>202105</creationdate><title>Nicotiana benthamiana LRR‐RLP NbEIX2 mediates the perception of an EIX‐like protein from Verticillium dahliae</title><author>Yin, Zhiyuan ; Wang, Nan ; Pi, Lei ; Li, Lei ; Duan, Weiwei ; Wang, Xiaodan ; Dou, Daolong</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c3871-91e682b9a137546dbb4e08b62b06e6e847f21ab5c4ea949a92970c1bc2c7eb173</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2021</creationdate><topic>Breeding</topic><topic>Cell death</topic><topic>ethylene‐inducing xylanase (EIX)</topic><topic>Gene expression</topic><topic>Leucine</topic><topic>microbe‐associated molecular pattern (MAMP)</topic><topic>Nicotiana benthamiana</topic><topic>Pathogens</topic><topic>Perception</topic><topic>Proteins</topic><topic>PRR</topic><topic>Receptors</topic><topic>Soil microorganisms</topic><topic>Verticillium dahliae</topic><topic>Verticillium wilt</topic><topic>Xylanase</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Yin, Zhiyuan</creatorcontrib><creatorcontrib>Wang, Nan</creatorcontrib><creatorcontrib>Pi, Lei</creatorcontrib><creatorcontrib>Li, Lei</creatorcontrib><creatorcontrib>Duan, Weiwei</creatorcontrib><creatorcontrib>Wang, Xiaodan</creatorcontrib><creatorcontrib>Dou, Daolong</creatorcontrib><collection>PubMed</collection><collection>CrossRef</collection><collection>Biotechnology Research Abstracts</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><collection>Wanfang Data Journals - Hong Kong</collection><collection>WANFANG Data Centre</collection><collection>Wanfang Data Journals</collection><collection>万方数据期刊 - 香港版</collection><collection>China Online Journals (COJ)</collection><collection>China Online Journals (COJ)</collection><jtitle>Journal of integrative plant biology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Yin, Zhiyuan</au><au>Wang, Nan</au><au>Pi, Lei</au><au>Li, Lei</au><au>Duan, Weiwei</au><au>Wang, Xiaodan</au><au>Dou, Daolong</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Nicotiana benthamiana LRR‐RLP NbEIX2 mediates the perception of an EIX‐like protein from Verticillium dahliae</atitle><jtitle>Journal of integrative plant biology</jtitle><addtitle>J Integr Plant Biol</addtitle><date>2021-05</date><risdate>2021</risdate><volume>63</volume><issue>5</issue><spage>949</spage><epage>960</epage><pages>949-960</pages><issn>1672-9072</issn><eissn>1744-7909</eissn><abstract>Verticillium wilt diseases caused by the soil‐borne fungus Verticillium dahliae result in devastating yield losses in many economically important crops annually. Here, we identified a novel ethylene‐inducing xylanase (EIX)‐like protein, VdEIX3, from V. dahliae, which exhibits immunity‐inducing activity in Nicotiana benthamiana. In vitro‐purified VdEIX3 can induce strong oxidative burst, activate the expression of defense‐related genes, and increase resistance against oomycete and fungal pathogens in N. benthamiana. VdEIX3 orthologs of other Verticillium pathogens also induce cell death in N. benthamiana, which form a new type of EIX protein family that is distinct from the known EIX proteins. A leucine‐rich repeat receptor‐like protein, NbEIX2, regulates the perception of VdEIX3 in N. benthamiana. Our results demonstrate that VdEIX3 is a novel EIX‐like protein that can be recognized by N. benthamiana NbEIX2, and also suggest that NbEIX2 is a promising receptor‐like protein that is potentially applicable to transgenic breeding for improving resistance to Verticillium wilt diseases.
Tomato plants detect Ethylene‐inducing xylanase (EIX) from the fungus Trichoderma viride and activate defense responses, but Nicotiana benthamiana plants do not detect EIX. Here, we identified an EIX‐like protein from the soil‐borne fungus Verticillium dahliae, VdEIX3, which is detected by the N. benthamiana receptor‐like protein NbEIX2.</abstract><cop>China (Republic : 1949- )</cop><pub>Wiley Subscription Services, Inc</pub><pmid>33205907</pmid><doi>10.1111/jipb.13031</doi><tpages>12</tpages></addata></record> |
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| subjects | Breeding Cell death ethylene‐inducing xylanase (EIX) Gene expression Leucine microbe‐associated molecular pattern (MAMP) Nicotiana benthamiana Pathogens Perception Proteins PRR Receptors Soil microorganisms Verticillium dahliae Verticillium wilt Xylanase |
| title | Nicotiana benthamiana LRR‐RLP NbEIX2 mediates the perception of an EIX‐like protein from Verticillium dahliae |
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