HP30-2, a mitochondrial PRAT protein for import of signal sequence-less precursor proteins in Arabidopsis thaliana
Chloroplasts and mitochondria contain a family of putative preprotein and amino acid transporters designated PRAT. Here, we analyzed the role of two previously characterized PRAT protein family members, encoded by At3g49560 （HP30） and At5g24650 （HP30-2）, in planta using a combination of genetic, cel...
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| Veröffentlicht in: | Journal of integrative plant biology 2017-08, Vol.59 (8), p.535-551 |
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| creator | Rossig, Claudia Gray, John Valdes, Oscar Rustgi, Sachin von Wettstein, Diter Reinbothe, Christiane Reinbothe, Steffen |
| description | Chloroplasts and mitochondria contain a family of putative preprotein and amino acid transporters designated PRAT. Here, we analyzed the role of two previously characterized PRAT protein family members, encoded by At3g49560 （HP30） and At5g24650 （HP30-2）, in planta using a combination of genetic, cell biological and biochemical approaches. Expression studies and green fluorescent protein tagging identified HP30-2 both in chloroplasts and mitochondria, whereas HP30 was located exclusively in chloroplasts. Biochemical evidence was obtained for an association of mitochondrial HP30-2 with two distinct protein complexes, one containing the inner membrane translocase TIM22 and the other containing an alternative NAD（P）H dehydrogenase subunit （NDCI） implicated in a respiratory complex 1-1ike electron trans- port chain. Through its association with TIM22, HP30-2 is involved in the uptake of carrier proteins and other, hydrophobic membrane proteins lacking cleavable N H2-terminal presequences, whereas HP30-2＇s interaction with NDC1 may permit controlling mitochondrial biogenesis and activity. |
| doi_str_mv | 10.1111/jipb.12555 |
| format | Article |
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Here, we analyzed the role of two previously characterized PRAT protein family members, encoded by At3g49560 （HP30） and At5g24650 （HP30-2）, in planta using a combination of genetic, cell biological and biochemical approaches. Expression studies and green fluorescent protein tagging identified HP30-2 both in chloroplasts and mitochondria, whereas HP30 was located exclusively in chloroplasts. Biochemical evidence was obtained for an association of mitochondrial HP30-2 with two distinct protein complexes, one containing the inner membrane translocase TIM22 and the other containing an alternative NAD（P）H dehydrogenase subunit （NDCI） implicated in a respiratory complex 1-1ike electron trans- port chain. Through its association with TIM22, HP30-2 is involved in the uptake of carrier proteins and other, hydrophobic membrane proteins lacking cleavable N H2-terminal presequences, whereas HP30-2＇s interaction with NDC1 may permit controlling mitochondrial biogenesis and activity.</description><identifier>ISSN: 1672-9072</identifier><identifier>EISSN: 1744-7909</identifier><identifier>DOI: 10.1111/jipb.12555</identifier><identifier>PMID: 28544763</identifier><language>eng</language><publisher>China (Republic : 1949- ): Wiley Subscription Services, Inc</publisher><subject>Amino Acid Transport Systems - metabolism ; Amino acids ; Arabidopsis - metabolism ; Arabidopsis Proteins - metabolism ; Chloroplasts ; Chloroplasts - metabolism ; Electron transport ; Electron transport chain ; Fluorescence ; Gene Silencing ; Green fluorescent protein ; Green Fluorescent Proteins - metabolism ; Hydrophobicity ; Membrane proteins ; Mitochondria ; Mitochondria - metabolism ; Mitochondrial Proteins - metabolism ; Multigene Family ; NAD ; NADPH dehydrogenase ; Precursors ; Protein Binding ; Protein Sorting Signals ; Protein transport ; Proteins ; Seedlings - metabolism ; Translocase ; 信号序列 ; 前体蛋白 ; 口部 ; 拟南芥 ; 线粒体蛋白 ; 细胞生物学 ; 绿色荧光蛋白标记 ; 载体蛋白</subject><ispartof>Journal of integrative plant biology, 2017-08, Vol.59 (8), p.535-551</ispartof><rights>2017 Institute of Botany, Chinese Academy of Sciences</rights><rights>2017 Institute of Botany, Chinese Academy of Sciences.</rights><rights>Copyright © Wanfang Data Co. 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Here, we analyzed the role of two previously characterized PRAT protein family members, encoded by At3g49560 （HP30） and At5g24650 （HP30-2）, in planta using a combination of genetic, cell biological and biochemical approaches. Expression studies and green fluorescent protein tagging identified HP30-2 both in chloroplasts and mitochondria, whereas HP30 was located exclusively in chloroplasts. Biochemical evidence was obtained for an association of mitochondrial HP30-2 with two distinct protein complexes, one containing the inner membrane translocase TIM22 and the other containing an alternative NAD（P）H dehydrogenase subunit （NDCI） implicated in a respiratory complex 1-1ike electron trans- port chain. Through its association with TIM22, HP30-2 is involved in the uptake of carrier proteins and other, hydrophobic membrane proteins lacking cleavable N H2-terminal presequences, whereas HP30-2＇s interaction with NDC1 may permit controlling mitochondrial biogenesis and activity.</description><subject>Amino Acid Transport Systems - metabolism</subject><subject>Amino acids</subject><subject>Arabidopsis - metabolism</subject><subject>Arabidopsis Proteins - metabolism</subject><subject>Chloroplasts</subject><subject>Chloroplasts - metabolism</subject><subject>Electron transport</subject><subject>Electron transport chain</subject><subject>Fluorescence</subject><subject>Gene Silencing</subject><subject>Green fluorescent protein</subject><subject>Green Fluorescent Proteins - metabolism</subject><subject>Hydrophobicity</subject><subject>Membrane proteins</subject><subject>Mitochondria</subject><subject>Mitochondria - metabolism</subject><subject>Mitochondrial Proteins - metabolism</subject><subject>Multigene Family</subject><subject>NAD</subject><subject>NADPH dehydrogenase</subject><subject>Precursors</subject><subject>Protein Binding</subject><subject>Protein Sorting Signals</subject><subject>Protein transport</subject><subject>Proteins</subject><subject>Seedlings - metabolism</subject><subject>Translocase</subject><subject>信号序列</subject><subject>前体蛋白</subject><subject>口部</subject><subject>拟南芥</subject><subject>线粒体蛋白</subject><subject>细胞生物学</subject><subject>绿色荧光蛋白标记</subject><subject>载体蛋白</subject><issn>1672-9072</issn><issn>1744-7909</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2017</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kktv1DAUhS1ERUthww9AFggJoab4FT-WQwW0VSVGqKwtJ3GmHiVxaicq5ddzy2S6YNG7sa_03aN7fIzQG0pOKdTnbRirU8rKsnyGjqgSolCGmOdwl4oVhih2iF7mvCWEayLZC3TIdCmEkvwIpfM1JwU7wQ73YYr1TRyaFFyH1z9X13hMcfJhwG1MOPRjTBOOLc5hMwCR_e3sh9oXnc8ZUF_PKQO4DGUMg6vkqtDEMYeMpxvXBTe4V-igdV32r5fzGP369vX67Ly4-vH94mx1VdSCirJgymkjmWey5JqCFV9Bz2vnDHO-Fa5hVFdem0a2hAomJC2b1jelphXXleDH6MNO984NrRs2dhvnBItn--fud8UIVUTDkwD3ccfB4uAoT7YPufZd5wYf52ypIZxKATCg7_9DHzWpgQCIFkoB9WlH1SnmnHxrxxR6l-4tJfYhMfuQmP2XGMBvF8m56n3ziO4jAoAuNkLn75-QspcX6y970XfLBpDn5jaA-f0cfAkjJBT_Cx6oqoE</recordid><startdate>201708</startdate><enddate>201708</enddate><creator>Rossig, Claudia</creator><creator>Gray, John</creator><creator>Valdes, Oscar</creator><creator>Rustgi, Sachin</creator><creator>von Wettstein, Diter</creator><creator>Reinbothe, Christiane</creator><creator>Reinbothe, Steffen</creator><general>Wiley Subscription Services, Inc</general><general>Department of Crop and Soil Sciences, Washington State University, Pullman WA 99164-6420, USA%Department of Crop and Soil Sciences, Washington State University, Pullman WA 99164-6420, USA</general><general>Laboratory of Plant Molecular Genetics and Laboratory of Environmental and Systems Biology, Grenoble-Alpes-University, Grenoble, France%Department of Biological Sciences, University of Toledo, 2801 West Bancroft Street, Toledo, OH 43606, USA%Department of Plant and Environmental Sciences, Pee Dee Research and Education Center, Clemson University, Florence, SC 29506, USA</general><scope>2RA</scope><scope>92L</scope><scope>CQIGP</scope><scope>W94</scope><scope>WU4</scope><scope>~WA</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QO</scope><scope>7T7</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope><scope>2B.</scope><scope>4A8</scope><scope>92I</scope><scope>93N</scope><scope>PSX</scope><scope>TCJ</scope></search><sort><creationdate>201708</creationdate><title>HP30-2, a mitochondrial PRAT protein for import of signal sequence-less precursor proteins in Arabidopsis thaliana</title><author>Rossig, Claudia ; 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Here, we analyzed the role of two previously characterized PRAT protein family members, encoded by At3g49560 （HP30） and At5g24650 （HP30-2）, in planta using a combination of genetic, cell biological and biochemical approaches. Expression studies and green fluorescent protein tagging identified HP30-2 both in chloroplasts and mitochondria, whereas HP30 was located exclusively in chloroplasts. Biochemical evidence was obtained for an association of mitochondrial HP30-2 with two distinct protein complexes, one containing the inner membrane translocase TIM22 and the other containing an alternative NAD（P）H dehydrogenase subunit （NDCI） implicated in a respiratory complex 1-1ike electron trans- port chain. Through its association with TIM22, HP30-2 is involved in the uptake of carrier proteins and other, hydrophobic membrane proteins lacking cleavable N H2-terminal presequences, whereas HP30-2＇s interaction with NDC1 may permit controlling mitochondrial biogenesis and activity.</abstract><cop>China (Republic : 1949- )</cop><pub>Wiley Subscription Services, Inc</pub><pmid>28544763</pmid><doi>10.1111/jipb.12555</doi><tpages>17</tpages></addata></record> |
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| ispartof | Journal of integrative plant biology, 2017-08, Vol.59 (8), p.535-551 |
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| source | MEDLINE; Wiley Online Library Journals Frontfile Complete; Alma/SFX Local Collection |
| subjects | Amino Acid Transport Systems - metabolism Amino acids Arabidopsis - metabolism Arabidopsis Proteins - metabolism Chloroplasts Chloroplasts - metabolism Electron transport Electron transport chain Fluorescence Gene Silencing Green fluorescent protein Green Fluorescent Proteins - metabolism Hydrophobicity Membrane proteins Mitochondria Mitochondria - metabolism Mitochondrial Proteins - metabolism Multigene Family NAD NADPH dehydrogenase Precursors Protein Binding Protein Sorting Signals Protein transport Proteins Seedlings - metabolism Translocase 信号序列 前体蛋白 口部 拟南芥 线粒体蛋白 细胞生物学 绿色荧光蛋白标记 载体蛋白 |
| title | HP30-2, a mitochondrial PRAT protein for import of signal sequence-less precursor proteins in Arabidopsis thaliana |
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