Digital imaging approaches for phenotyping whole plant nitrogen and phosphorus response in Brachypodium distachyon

This work evaluates the phenotypic response of the model grass (Brachypodium distachyon (L.) P. Beauv.) to nitrogen and phosphorus nutrition using a combination of imaging techniques and destructive harvest of shoots and roots. Reference line Bd21‐3 was grown in pots using 11 phosphorus and 11 nitro...

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Veröffentlicht in:Journal of integrative plant biology 2014-08, Vol.56 (8), p.781-796
Hauptverfasser: Poiré, Richard, Chochois, Vincent, Sirault, Xavier R. R, Vogel, John P, Watt, Michelle, Furbank, Robert T
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container_issue 8
container_start_page 781
container_title Journal of integrative plant biology
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creator Poiré, Richard
Chochois, Vincent
Sirault, Xavier R. R
Vogel, John P
Watt, Michelle
Furbank, Robert T
description This work evaluates the phenotypic response of the model grass (Brachypodium distachyon (L.) P. Beauv.) to nitrogen and phosphorus nutrition using a combination of imaging techniques and destructive harvest of shoots and roots. Reference line Bd21‐3 was grown in pots using 11 phosphorus and 11 nitrogen concentrations to establish a dose–response curve. Shoot biovolume and biomass, root length and biomass, and tissue phosphorus and nitrogen concentrations increased with nutrient concentration. Shoot biovolume, estimated by imaging, was highly correlated with dry weight (R² > 0.92) and both biovolume and growth rate responded strongly to nutrient availability. Higher nutrient supply increased nodal root length more than other root types. Photochemical efficiency was strongly reduced by low phosphorus concentrations as early as 1 week after germination, suggesting that this measurement may be suitable for high throughput screening of phosphorus response. In contrast, nitrogen concentration had little effect on photochemical efficiency. Changes in biovolume over time were used to compare growth rates of four accessions in response to nitrogen and phosphorus supply. We demonstrate that a time series image‐based approach coupled with mathematical modeling provides higher resolution of genotypic response to nutrient supply than traditional destructive techniques and shows promise for high throughput screening and determination of genomic regions associated with superior nutrient use efficiency.
doi_str_mv 10.1111/jipb.12198
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subjects Biomass
Brachypodium - growth & development
Brachypodium - metabolism
Brachypodium distachyon
germination
grasses
image analysis
Image Processing, Computer-Assisted
mathematical models
Models, Biological
nitrogen
Nitrogen - metabolism
nitrogen content
nutrient availability
nutrient content
nutrient use efficiency
Nutrients uptake
nutrition
phenomics
Phenotype
phosphorus
Phosphorus - metabolism
Photosynthesis
Plant Roots - growth & development
Plant Shoots - growth & development
root
roots
screening
shoot
shoots
time series analysis
title Digital imaging approaches for phenotyping whole plant nitrogen and phosphorus response in Brachypodium distachyon
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